CN206594051U - A kind of system for detecting choline glycerophosphatide - Google Patents
A kind of system for detecting choline glycerophosphatide Download PDFInfo
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- CN206594051U CN206594051U CN201720234760.8U CN201720234760U CN206594051U CN 206594051 U CN206594051 U CN 206594051U CN 201720234760 U CN201720234760 U CN 201720234760U CN 206594051 U CN206594051 U CN 206594051U
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- open end
- porous carrier
- pretreatment unit
- choline glycerophosphatide
- phosphorus
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Abstract
The utility model is related to a kind of system for detecting choline glycerophosphatide, and the system includes thermostat, pretreatment unit, optical detection apparatus, and data processing equipment, it is characterised in that the pretreatment unit includes the first open end (11,21)；Second open end (12,22))；Outer wall (13,23)；Inwall (14,24)；The hollow channel (17,27) limited by the first open end, the second open end and inwall；The first porous carrier (15,25) for the immobilization glycerophosphocholine phosphodiesterase being filled within the hollow channel and the second porous carrier (16,26) of Immobilized Alkaline Phosphatase；And removably seal the seal (18,28) of at least one of the first open end and the second open end.The beneficial effects of the utility model are to repeat using catabolic enzyme, detection time is short and pretreatment unit is readily disassembled assembling and clean and maintenance.
Description
Technical field
The utility model belongs to biochemistry detection apparatus field.Specifically, the utility model is related to a kind of detection glycerine phosphorus
The system of sour choline, the system includes the porous load containing immobilization glycerophosphocholine phosphodiesterase and alkaline phosphatase
The pretreatment unit of body.
Background technology
Choline glycerophosphatide is the soluble small molecular existed in human body, may there is certain associate with many diseases.
Therefore, research and detection to choline glycerophosphatide is of increasing concern.The chemical structural formula of choline glycerophosphatide is as follows：
1 glycerophosphocholine molecules, which is can be seen that, from structural formula above comprises only 1 phosphorus (P) atom.In other words
Say, the molar concentration of phosphorus atoms is equal to the molar concentration of choline glycerophosphatide.So when detecting choline glycerophosphatide, can pass through
The molar concentration of phosphorus atoms in choline glycerophosphatide catabolite is determined to detect the molar concentration of choline glycerophosphatide.
Chinese patent application CN105784980 A disclose a kind of method for detecting choline glycerophosphatide, methods described
Technology path is that choline glycerophosphatide is degraded into glycerol 3-phosphate and choline by glycerophosphocholine phosphodiesterase；Pass through
Glycerol 3-phosphate is degraded into glycerine and Phos by alkaline phosphatase；The dense of Phos is determined by conventional molybdenum blue method
Degree；And the concentration of choline glycerophosphatide is obtained according to the concentration of Phos.This method, which is disadvantageous in that, makes two kinds points
Solve enzyme directly to react with testing sample, reaction is difficult to reclaim and reuse catabolic enzyme after terminating.
In zymetology field, enzyme can be reused by porous particle or perforated membrane immobilised enzymes or the stability of enzyme is improved
(for example, with reference to Tan Tianwei etc., " with lipase Immobilized on Diatomite and enzymatic clarification monoglyceride ",《Chinese grain and oil journal》,
2000,15(2):29-31；It is just shining etc., " research of porous ceramic film fixed papain ",《Agricultural University Of South China's journal》,
2006,27(4):36-39；And Sui Ying, " progress of inorganic Carrier Materials of Immobilized Enzyme ",《Shandong chemical industry》,2013,42
(7):67-68 etc.).But the enzyme immobilization technology in existing research is generally used for single enzyme reaction system.
Therefore, this area remains a need for a kind of repeatable utilization catabolic enzyme and the short detection phosphoglycerol of detection time
The system of choline.
Utility model content
The purpose of this utility model, which is that offer is a kind of, includes the pre- place of the porous carrier containing two kinds of catabolic enzymes of immobilization
The system for managing the detection choline glycerophosphatide of device, so that the problem of solving to propose in background technology.
To achieve these goals, the utility model provides following technical proposals：
A kind of system for detecting choline glycerophosphatide, the system may include thermostat, pretreatment unit, optical detection
Device, and data processing equipment, the pretreatment unit are configured to the glycerine in the testing sample containing choline glycerophosphatide
Phosphocholine is degraded into the catabolite containing Phos, and the optical detection apparatus is configured to determine described by molybdenum blue method
Absorbance of the catabolite containing Phos under 660 nano wave lengths, the data processing equipment is configured to according to optical detection
Absorbance of the catabolite containing Phos under 660 nano wave lengths that device is determined determines the degraded production containing Phos
In thing in the molar concentration of Phos and the testing sample containing choline glycerophosphatide choline glycerophosphatide molar concentration,
Characterized in that, the pretreatment unit includes the first open end；Second open end；Outer wall；Inwall；By the first openend
The hollow channel that portion, the second open end and inwall are limited；It is filled in the immobilization phosphoglycerol courage within the hollow channel
First porous carrier of alkali phosphodiesterase and the second porous carrier of Immobilized Alkaline Phosphatase；And removably seal
The seal of at least one of one open end and the second open end.
In one embodiment, the thermostat is that water-bath, oil bath or the electricity around pretreatment unit outer wall add
Hot device.
In one embodiment, the optical detection apparatus may include ultraviolet-uisible spectrophotometer.
In one embodiment, the data processing equipment may include computer, and the computer, which can be stored, uses phosphorus molybdenum
Blue laws determines the working curve of phosphorus molar concentration according to the absorbance under 660 nano wave lengths when carrying out quantitative to phosphorus.
In one embodiment, first porous carrier and the second porous carrier independently selected from activated carbon granule,
Alumina particle, diatomite particle, porous ceramic particles or cellular glass particle.
In one embodiment, first porous carrier and the second porous carrier may include porous ceramic film.
In one embodiment, the porous carrier fills up the hollow channel.
In one embodiment, the cross section of the hollow channel has circular, rectangle or elliptical shape.
In one embodiment, the seal may include glass stopper.
Compared with prior art, the beneficial effects of the utility model be it is repeatable using catabolic enzyme, detection time it is short and
Pretreatment unit is readily disassembled assembling and clean and maintenance.
Brief description of the drawings
Fig. 1 is a kind of cross section structure schematic diagram of the detachable pretreatment unit of embodiment of the present utility model.
Fig. 2 is the cross section structure schematic diagram of the detachable pretreatment unit of another embodiment of the present utility model.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clear and complete description.It will be recognized by one of ordinary skill in the art that the size and practical structures of accompanying drawing are disproportionate, in order to more
Feature and advantage of the present utility model are clearly illustrated, may exaggerate or reduce the size of some structures.
Embodiment 1
In this embodiment there is provided a kind of system for detecting choline glycerophosphatide, the system may include water-bath, and glass is located in advance
Reason pipe, ultraviolet specrophotometer, and computer.Water-bath is used to provide suitable glycerine phosphocholine phosphodiesterase and alkaline phosphorus
The temperature of sour enzyme reaction, such as 37 DEG C.Glass pretreated tube is used for the glycerine phosphorus in the testing sample containing choline glycerophosphatide
Sour choline is degraded into the catabolite containing Phos.Ultraviolet specrophotometer is used to determine containing Phos by molybdenum blue method
Absorbance of the catabolite under 660 nano wave lengths.Computer stored is received when carrying out quantitative to phosphorus with molybdenum blue method according to 660
Absorbance under metric wave length determines the working curve of phosphorus molar concentration, and for according to optical detection apparatus determine containing inorganic
Absorbance of the catabolite of phosphorus under 660 nano wave lengths determines the mole dense of Phos in the catabolite containing Phos
Degree.Because 1 glycerophosphocholine molecules comprises only 1 phosphorus atoms, mole of the Phos determined by working curve
Concentration is the molar concentration of choline glycerophosphatide in the testing sample containing choline glycerophosphatide.One example of working curve can
The method according to disclosed in Chinese patent application CN105784980 A is obtained, and can be represented by following formula (1)：
Choline glycerophosphatide molar concentration=phosphorus molar concentration=0.0756 × phosphorus absorbance (1) under 660 nano wave lengths
Fig. 1 is the cross section structure schematic diagram of glass pretreated tube 10 of the present utility model.With reference to Fig. 1, glass pretreated tube
10 include the first open end 11；Second open end 12；Outer wall 13；Inwall 14；By the first open end 11, the second openend
The hollow channel 17 that portion 12 and inwall 14 are limited；It is filled in the immobilization choline glycerophosphatide di(2-ethylhexyl)phosphate within the hollow channel
Esterase (No. CAS:Diatomite particle 15 9001-86-9) and immobilization calf intestinal alkaline phosphatase (NEW ENGLAND,Company, article No.：M0290L cellular glass particle 16)；And removably seal the glass of the first open end 11
Glass plug 18.The cross section of the glass pretreated tube 10 is circle.It is noted that in order to clearly illustrate detachable pretreatment unit
10 structure, only shows that glass stopper 18 not yet seals the embodiment of the first open end 11.Those skilled in the art can hold
Change places and obtain the embodiment that glass stopper 18 seals the first open end 11 and/or the second open end 12.
Embodiment 2
In this embodiment there is provided the system of another detection choline glycerophosphatide, the system may include electric heater, glass
Glass pretreated tube, ultraviolet specrophotometer, and computer.Wherein ultraviolet specrophotometer and computer and embodiment 1
It is identical.
Fig. 2 is the cross section structure schematic diagram of glass pretreated tube 20 of the present utility model.With reference to Fig. 2, glass pretreated tube
20 include the first open end 21；Second open end 22；Outer wall 23；Inwall 24；By the first open end 21, the second openend
The hollow channel 27 that portion 22 and inwall 24 are limited；It is filled in the immobilization choline glycerophosphatide di(2-ethylhexyl)phosphate within the hollow channel
Esterase (No. CAS:Porous ceramic film 25 9001-86-9) and immobilization calf intestinal alkaline phosphatase (NEW ENGLAND,Company, article No.：M0290L porous ceramic film 26)；And removably seal the glass of the first open end 21
Plug 28.The cross section of the glass pretreated tube 20 is circle.It is noted that in order to clearly illustrate detachable pretreatment unit 20
Structure, only display glass stopper 28 not yet seal the embodiment of the first open end 21.Those skilled in the art can be easy
Ground obtains the embodiment that glass stopper 28 seals the first open end 21 and/or the second open end 22.
In addition, in the present embodiment, thermostat is the electric heater 29 around the outer wall 24 of glass pretreated tube 20.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and
In the case of without departing substantially from spirit or essential attributes of the present utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, embodiment should be regarded as exemplary, rather than it is restricted, protection domain of the present utility model is by appended power
Sharp claim is limited.So, all changes fallen in the implication and scope of the equivalency of claim are included in this reality
Within new protection domain.And any reference in claim should not be considered as limitation involved by right will
Ask.
Furthermore, it is to be understood that although the present specification is described in terms of embodiments, not each embodiment is only included
One independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should be by
Specification is as an entirety, and technical characteristic or technical scheme in each embodiment can be combined, and form this area skill
Art personnel may be appreciated other embodiment.
Claims (8)
1. a kind of system for detecting choline glycerophosphatide, the system includes thermostat, pretreatment unit, optical detection dress
Put, and data processing equipment, the pretreatment unit is configured to the glycerine phosphorus in the testing sample containing choline glycerophosphatide
Sour choline is degraded into the catabolite containing Phos, and the optical detection apparatus is configured to determine degraded production by molybdenum blue method
Absorbance of the thing under 660 nano wave lengths, the data processing equipment be configured to according under 660 nano wave lengths absorbance come
The molar concentration of choline glycerophosphatide in the molar concentration and testing sample of Phos in catabolite is determined, its feature exists
In the pretreatment unit includes the first open end (11,21)；Second open end (12,22))；Outer wall (13,23)；It is interior
Wall (14,24)；The hollow channel (17,27) limited by the first open end, the second open end and inwall；Be filled in it is described in
The first porous carrier (15,25) and immobilization alkaline phosphatase of immobilization glycerophosphocholine phosphodiesterase within empty passage
The second porous carrier (16,26) of enzyme；And removably seal at least one of the first open end and the second open end
Seal (18,28).
2. the system as claimed in claim 1, it is characterised in that the thermostat includes water-bath, oil bath or around pre- place
Manage the electric heater (29) of device outer wall (13,23).
3. the system as claimed in claim 1, it is characterised in that the optical detection apparatus includes UV, visible light spectrophotometric
Meter.
4. the system as claimed in claim 1, it is characterised in that the data processing equipment includes computer, the computer
Store the work song for determining phosphorus molar concentration when carrying out quantitative to phosphorus with molybdenum blue method according to the absorbance under 660 nano wave lengths
Line.
5. the system as claimed in claim 1, it is characterised in that first porous carrier and the second porous carrier are independently selected
From activated carbon granule, alumina particle, diatomite particle, porous ceramic particles or cellular glass particle.
6. the system as claimed in claim 1, it is characterised in that first porous carrier and the second porous carrier include porous
Ceramic membrane.
7. the system as claimed in claim 1, it is characterised in that the cross section of the hollow channel has circular, rectangle or ellipse
It is round-shaped.
8. the system as claimed in claim 1, it is characterised in that the seal includes glass stopper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720234760.8U CN206594051U (en) | 2017-03-10 | 2017-03-10 | A kind of system for detecting choline glycerophosphatide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720234760.8U CN206594051U (en) | 2017-03-10 | 2017-03-10 | A kind of system for detecting choline glycerophosphatide |
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| Publication Number | Publication Date |
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| CN206594051U true CN206594051U (en) | 2017-10-27 |
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| CN201720234760.8U Active CN206594051U (en) | 2017-03-10 | 2017-03-10 | A kind of system for detecting choline glycerophosphatide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107631988A (en) * | 2017-09-26 | 2018-01-26 | 深圳市瑞赛生物技术有限公司 | A kind of method and its kit of quick detection choline/acetylcholine |
-
2017
- 2017-03-10 CN CN201720234760.8U patent/CN206594051U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107631988A (en) * | 2017-09-26 | 2018-01-26 | 深圳市瑞赛生物技术有限公司 | A kind of method and its kit of quick detection choline/acetylcholine |
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