CN206566772U - Membrane separation device for polypeptide fractionation - Google Patents

Membrane separation device for polypeptide fractionation Download PDF

Info

Publication number
CN206566772U
CN206566772U CN201720032151.4U CN201720032151U CN206566772U CN 206566772 U CN206566772 U CN 206566772U CN 201720032151 U CN201720032151 U CN 201720032151U CN 206566772 U CN206566772 U CN 206566772U
Authority
CN
China
Prior art keywords
tubular
membrane
classified
separation
hollow fiber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720032151.4U
Other languages
Chinese (zh)
Inventor
李志华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lekang Zhentai Tianjin Biotechnology Co ltd
Original Assignee
Lekang Zhentai Tianjin Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lekang Zhentai Tianjin Biotechnology Co ltd filed Critical Lekang Zhentai Tianjin Biotechnology Co ltd
Priority to CN201720032151.4U priority Critical patent/CN206566772U/en
Application granted granted Critical
Publication of CN206566772U publication Critical patent/CN206566772U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The utility model provides a membrane separation device for polypeptide fractionation, including storage case and stage filtration membrane system, the storage case lower extreme is equipped with the discharge gate, stage filtration membrane system includes tubular ultrafiltration membrane subassembly, external pressure type hollow fiber ultrafiltration membrane subassembly and tubular nanofiltration membrane subassembly, tubular ultrafiltration membrane subassembly, external pressure type hollow fiber ultrafiltration membrane subassembly and tubular nanofiltration membrane subassembly series connection, tubular ultrafiltration membrane subassembly comprises a plurality of tubular ultrafiltration chimney filters, tubular ultrafiltration chimney filters's molecular weight cutoff is 3000 dalton, external pressure type hollow fiber ultrafiltration membrane subassembly comprises a plurality of hollow fiber chimney filters, hollow fiber chimney filters ' molecular weight cutoff is 1000 dalton, tubular nanofiltration membrane subassembly comprises a plurality of tubular nanofiltration chimney filters, tubular nanofiltration chimney filters's molecular weight cutoff is 500 dalton, the utility model discloses a membrane subassembly of different molecular weight cutoffs carries out stage separation to mixed polypeptide liquid, has the advantages of large membrane flux, high separation efficiency, good separation effect and the like.

Description

A kind of membrane separation device that separation is classified for polypeptide
Technical field
The utility model belongs to technical field of membrane separation, is filled more particularly, to a kind of UF membrane for being classified separation for polypeptide Put.
Background technology
The peptide fragment containing different molecular weight in polypeptide, enzymolysis liquid can be made in protein after proteases for decomposing, through research It was found that, below 3000Da polypeptide has a bioactivity, and the bioactivity that has of polypeptide also each not phase of different molecular weight Together, therefore, it is very necessary that the polypeptide for different molecular weight, which carries out efficiently separating purification, is normally applied in the prior art Membrane separating method is separated to the polypeptide of different molecular weight.
UF membrane is to pass through film as separating medium using selectivity, in the presence of the certain motive force in film both sides, is made in raw material Certain compositional selecting pass through film so that mixture is separated, the purposes such as purification, concentration are reached, due to UF membrane skill Art energy consumption is low, good separating effect the advantages of be widely used, therefore UF membrane is applied to food processing field by researcher, right Protein enzyme solution is isolated and purified, but current film filter is mostly single-stage, it is often necessary to through a kind of membrane filtration Afterwards, the filtering for carrying out next step after filter membrane again is changed, it is necessary to can just obtain the component of required molecular weight ranges by multiple times of filtration, Cumbersome, efficiency is low, the purpose of series classification separation can not be played to polypeptide.
The content of the invention
Problem to be solved in the utility model be to provide a kind of simple to operate, separative efficiency it is high, being capable of series classification separation Be used for polypeptide be classified separation membrane separation device.
In order to solve the above technical problems, the technical solution adopted in the utility model is:It is a kind of to be classified separation for polypeptide Membrane separation device, including material storing box and classified filtering membranous system, the material storing box upper end is provided with charging aperture, the material storing box End is provided with discharging opening, and the classified filtering membranous system is connected with the discharging opening, and the classified filtering membranous system is from left to right Include tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component, the tubular type ultrafiltration successively Membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component are connected in series, the tubular ultrafiltration membrane module by Several tubular type ultra filtration chimney filters are constituted, and the molecular cut off of the tubular type ultra filtration chimney filter is 3000 dalton, the external pressure Formula polysulfone hollow fiber ultrafiltration membrane system is made up of several hollow fibre filtering pipes, the molecular cut off of the hollow fibre filtering pipe For 1000 dalton, the tubular nanofiltration membrane component is made up of several tubular type nanofiltration screen pipes, the tubular type nanofiltration screen pipe Molecular cut off be 500 dalton.
Further, the tubular ultrafiltration membrane module upper end is provided with the first inlet, the tubular ultrafiltration membrane module End be provided with the first trapped fluid outlet, tubular ultrafiltration membrane module side lower end be provided with the first permeate outlet, it is described go out Material mouth is connected with first inlet by the first conduit, and first switch valve, first pressure are provided with first conduit Table and the first membrane pump.
Further, the first trapped fluid exit is provided with the first bleeder valve.
Further, the side upper end of the external compression type hollow fiber ultrafiltration membrane component is provided with the second inlet, described The side lower end of external compression type hollow fiber ultrafiltration membrane component is provided with the outlet of the second trapped fluid, the hollow fiber ultrafiltration membrane group Part lower end is provided with the outlet of the second permeate, and the first permeate outlet is connected with second inlet by the second conduit Connect, second switch valve, second pressure table and the second membrane pump are provided with second conduit.
Further, the second trapped fluid exit is provided with the second bleeder valve.
Further, the upper end of the tubular nanofiltration membrane component is provided with the 3rd inlet, the tubular nanofiltration membrane component Lower end be provided with the outlet of the 3rd trapped fluid, the side lower end of the tubular nanofiltration membrane component is provided with the outlet of the 3rd permeate, The second permeate outlet is connected with the 3rd inlet by the 3rd conduit, and being provided with the 3rd on the 3rd conduit opens Close valve, the 3rd pressure gauge and the 3rd membrane pump.
Further, the 3rd trapped fluid exit is provided with the 3rd bleeder valve.
Further, the 3rd permeate outlet is connected by the 4th conduit with the material storing box.
Further, it is provided with the storing chamber interior wall on liquid level sensor, the storing box outer wall and is provided with alarm Device, the liquid level sensor is electrically connected with the warning device.
Compared with prior art, the utility model, which is had the advantage that with beneficial effect, is:
1st, the utility model uses tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane group The mode that part is serially connected, according to the difference of membrane module molecular cut off, is separated, together step by step to the polypeptide of different molecular weight Shi Yingyong tubular membranes and external pressing type hollow fibrous membrane are as main membrane module form, with film surface area is big, flux is big, operation The advantages of pressure difference is relatively low, and integral device is simple in construction, good separating effect;
The 2nd, liquid level sensor is set in material storing box, while liquid level sensor is electrically connected with volume warning device on storing box outer wall Connect, when liquid level does not reach the level value of liquid level sensor setting in material storing box, control warning device alarm can be reminded in time Level condition in material storing box, it is safe.
Brief description of the drawings
Fig. 1 is a kind of structural representation for the membrane separation device that separation is classified for polypeptide of the utility model.
In figure:1- material storing box;2- charging apertures;3- discharging openings;4- tubular ultrafiltration membrane modules;5- external-compression type Hollow Fiber Ultrafiltrations Membrane module;6- tubular nanofiltration membrane components;7- tubular type ultra filtration chimney filters;8- hollow fibre filtering pipes;9- tubular type nanofiltration screen pipes; The inlets of 10- first;The trapped fluids of 11- first are exported;The permeate of 12- first is exported;13- first switch valves;14- first pressures Table;The membrane pumps of 15- first;The bleeder valves of 16- first;The conduits of 17- first;The inlets of 18- second;The trapped fluids of 19- second are exported; The permeate of 20- second is exported;The conduits of 21- second;22- second switch valves;23- second pressure tables;The membrane pumps of 24- second;25- Two bleeder valves;The inlets of 26- the 3rd;The trapped fluids of 27- the 3rd are exported;The permeate of 28- the 3rd is exported;The conduits of 29- the 3rd;30- Three switch valves;The pressure gauges of 31- the 3rd;The membrane pumps of 32- the 3rd;The bleeder valves of 33- the 3rd;The conduits of 34- the 4th;35- liquid level sensors; 36- warning devices.
Embodiment
Embodiment of the present utility model is elaborated below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of membrane separation device that separation is classified for polypeptide, including material storing box 1 and classified filtering membrane system System, the upper end of material storing box 1 is provided with charging aperture 2, and the lower end of material storing box 1 is provided with discharging opening 3, classified filtering membranous system and discharging opening 3 Connection, classified filtering membranous system includes tubular ultrafiltration membrane module 4, external compression type hollow fiber ultrafiltration membrane component 5 successively from left to right With tubular nanofiltration membrane component 6, tubular ultrafiltration membrane module 4, external compression type hollow fiber ultrafiltration membrane component 5 and tubular nanofiltration membrane component 6 It is connected in series, the upper end of tubular ultrafiltration membrane module 4 is provided with the first inlet 10, the lower end of tubular ultrafiltration membrane module 4 is provided with first Trapped fluid outlet 11, the side lower end of tubular ultrafiltration membrane module 4 is provided with the first permeate outlet 12, the feed liquor of discharging opening 3 and first Mouth 10 is connected by the first conduit 17, and first switch valve 13, the barrier film of first pressure table 14 and first are provided with the first conduit 17 Pump 15, the side upper end of external compression type hollow fiber ultrafiltration membrane component 5 is provided with the second inlet 18, external-compression type Hollow Fiber Ultrafiltration The side lower end of membrane module 5 is provided with the second trapped fluid outlet 19, and the lower end of polysulfone hollow fiber ultrafiltration membrane system 5 is provided with the second transmission Liquid outlet 20, the first permeate outlet 12 is connected with the second inlet 18 by the second conduit 21, is provided with the second conduit 21 Second switch valve 22, the membrane pump 24 of second pressure table 23 and second, the upper end of tubular nanofiltration membrane component 6 is provided with the 3rd inlet 26, the lower end of tubular nanofiltration membrane component 6 is provided with the 3rd trapped fluid outlet 27, and the side lower end of tubular nanofiltration membrane component 6 is set There is the 3rd permeate to export 28, the second permeate outlet 20 is connected with the 3rd inlet 26 by the 3rd conduit 29, the 3rd conduit The 3rd switch valve 30, the 3rd pressure gauge 31 and the 3rd membrane pump 32 are provided with 29, the 3rd permeate outlet 28 is led by the 4th Pipe 34 is connected with material storing box 1, circulation loop is formed between material storing box 1 and classified filtering membranous system, polypeptide mixed liquor is through undue During level filtration membrane systems, what is flowed out in trapped fluid exit is the polypeptide solution of required molecular weight.
Wherein, tubular ultrafiltration membrane module 4 is made up of several tubular type ultra filtration chimney filters 7, and tubular type ultra filtration chimney filter 7 can be The hydrophilic film of the materials such as makrolon, Kynoar or polyamide, the molecular cut off of tubular type ultra filtration chimney filter 7 is 3000 Dalton, external compression type hollow fiber ultrafiltration membrane component 5 is made up of several hollow fibre filtering pipes 8, and hollow fibre filtering pipe 8 can Think the hydrophilic film of the materials such as makrolon, Kynoar, polyamide or polyacrylonitrile, section of hollow fibre filtering pipe 8 It is 1000 dalton to stay molecular weight, and tubular nanofiltration membrane component 6 is made up of several tubular type nanofiltration screen pipes 9, tubular type nanofiltration filtering Pipe 9 can be aromatic polyamides composite membrane material or zirconium dioxide-PEI organic-inorganic nanofiltration membrane material, tubular type nanofiltration The molecular cut off of screen pipe 9 is 500 dalton, can prevent to form moisture film on film surface using hydrophilic film material, it is ensured that The stability of membrane flux, improves filter efficiency, and mixtures of polypeptides is effectively divided difference by the membrane module of PSPP The peptide separation of son amount comes out, good separating effect.
The course of work of the present utility model is:Polypeptide mixed liquor is placed into material storing box 1 first, first switch valve is opened 13, start the first membrane pump 15, polypeptide mixed liquor is pumped into tubular ultrafiltration membrane module 4, control first switch valve 13, control The filter pressure of tubular membrane component 4 is between 0.2MPa-0.7MPa, because the molecular cut off of tubular ultrafiltration membrane module 4 is 3000 dalton, can will be greater than the polypeptide retention of 3000 Dalton molecular weights, be collected at the first trapped fluid outlet 11, The polypeptide that molecular weight is less than 3000 dalton enters external-compression type by tubular ultrafiltration membrane module 4 by the first permeate outlet 12 In hollow fiber film assembly 5, by controlling the unlatching size of second switch valve 22 to control the mistake of external pressure type hollow fiber membrane component 5 Filtering pressure power is between 0.5MPa-0.8MPa, and the molecular cut off of external pressure type hollow fiber membrane component 5 is 1000 dalton, therefore Peptide molecule more than 1000 dalton is trapped, and is collected at the second trapped fluid outlet 19, permeate is saturating by second Cross liquid outlet 19 to enter in tubular nanofiltration membrane component 6, control the 3rd switch valve 30, tubular type nanofiltration membrane component 6 is controlled with this Filter pressure is between 0.1MPa-0.3MPa, and the molecular cut off of tubular nanofiltration membrane component 6 is 500 dalton, therefore is more than The peptide molecule of 500 dalton is trapped, and is collected at the 3rd trapped fluid outlet 27, permeate passes through the 3rd permeate 27 Outlet is come back in material storing box 1, and the liquid level sensor 35 carried out again in circulation classification separation process, material storing box 1 is detected When liquid level in material storing box 1 is less than setting value, control warning device 36 is alarmed, it is ensured that the security of the equipment course of work.
One embodiment of the present utility model is described in detail above, but the content is only of the present utility model Preferred embodiment, it is impossible to be considered as being used to limit practical range of the present utility model.It is all to be made according to present utility model application scope Equivalent change with improve etc., all should still belong within patent covering scope of the present utility model.

Claims (9)

1. a kind of membrane separation device that separation is classified for polypeptide, including material storing box (1) and classified filtering membranous system, the storing Case (1) upper end is provided with charging aperture (2), and material storing box (1) lower end is provided with discharging opening (3), the classified filtering membranous system It is connected with the discharging opening (3), it is characterised in that:The classified filtering membranous system includes tubular ultra-filtration membrane group successively from left to right Part (4), external compression type hollow fiber ultrafiltration membrane component (5) and tubular nanofiltration membrane component (6), it is the tubular ultrafiltration membrane module (4), outer Pressure type hollow fiber hyperfiltration membrane assembly (5) and tubular nanofiltration membrane component (6) are connected in series, the tubular ultrafiltration membrane module (4) by Several tubular type ultra filtration chimney filters (7) are constituted, and the molecular cut off of the tubular type ultra filtration chimney filter (7) is 3000 dalton, institute State external compression type hollow fiber ultrafiltration membrane component (5) to be made up of several hollow fibre filtering pipes (8), the hollow fibre filtering pipe (8) molecular cut off is 1000 dalton, and the tubular nanofiltration membrane component (6) is by several tubular type nanofiltration screen pipe (9) groups Into the molecular cut off of the tubular type nanofiltration screen pipe (9) is 500 dalton.
2. a kind of membrane separation device that separation is classified for polypeptide according to claim 1, it is characterised in that:The tubular type Hyperfiltration membrane assembly (4) upper end is provided with the first inlet (10), and tubular ultrafiltration membrane module (4) lower end is provided with the first retention Liquid exports (11), and tubular ultrafiltration membrane module (4) the side lower end is provided with the first permeate outlet (12), the discharging opening (3) it is connected with first inlet (10) by the first conduit (17), first switch is provided with first conduit (17) Valve (13), first pressure table (14) and the first membrane pump (15).
3. a kind of membrane separation device that separation is classified for polypeptide according to claim 2, it is characterised in that:Described first Trapped fluid outlet (11) place is provided with the first bleeder valve (16).
4. a kind of membrane separation device that separation is classified for polypeptide according to claim 2, it is characterised in that:The external pressure The side upper end of formula polysulfone hollow fiber ultrafiltration membrane system (5) is provided with the second inlet (18), the external-compression type Hollow Fiber Ultrafiltration The side lower end of membrane module (5) is provided with the second trapped fluid outlet (19), and polysulfone hollow fiber ultrafiltration membrane system (5) lower end is set There is the second permeate to export (20), the first permeate outlet (12) passes through the second conduit with second inlet (18) (21) connect, second switch valve (22), second pressure table (23) and the second membrane pump are provided with second conduit (21) (24)。
5. a kind of membrane separation device that separation is classified for polypeptide according to claim 4, it is characterised in that:Described second Trapped fluid outlet (19) place is provided with the second bleeder valve (25).
6. a kind of membrane separation device that separation is classified for polypeptide according to claim 4, it is characterised in that:The tubular type The upper end of nanofiltration membrane component (6) is provided with the 3rd inlet (26), and the lower end of the tubular nanofiltration membrane component (6) is provided with the 3rd Trapped fluid exports (27), and the side lower end of the tubular nanofiltration membrane component (6) is provided with the 3rd permeate outlet (28), and described the Two permeate outlet (20) is connected with the 3rd inlet (26) by the 3rd conduit (29), is set on the 3rd conduit (29) It is equipped with the 3rd switch valve (30), the 3rd pressure gauge (31) and the 3rd membrane pump (32).
7. a kind of membrane separation device that separation is classified for polypeptide according to claim 6, it is characterised in that:Described 3rd Trapped fluid outlet (27) place is provided with the 3rd bleeder valve (33).
8. a kind of membrane separation device that separation is classified for polypeptide according to claim 6, it is characterised in that:Described 3rd Permeate outlet (28) is connected by the 4th conduit (34) with the material storing box (1).
9. a kind of membrane separation device that separation is classified for polypeptide according to claim 1, it is characterised in that:The storing It is provided with case (1) inwall on liquid level sensor (35), material storing box (1) outer wall and is provided with warning device (36), the liquid Level sensor (35) is electrically connected with the warning device (36).
CN201720032151.4U 2017-01-11 2017-01-11 Membrane separation device for polypeptide fractionation Expired - Fee Related CN206566772U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720032151.4U CN206566772U (en) 2017-01-11 2017-01-11 Membrane separation device for polypeptide fractionation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720032151.4U CN206566772U (en) 2017-01-11 2017-01-11 Membrane separation device for polypeptide fractionation

Publications (1)

Publication Number Publication Date
CN206566772U true CN206566772U (en) 2017-10-20

Family

ID=60061454

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720032151.4U Expired - Fee Related CN206566772U (en) 2017-01-11 2017-01-11 Membrane separation device for polypeptide fractionation

Country Status (1)

Country Link
CN (1) CN206566772U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109758912A (en) * 2019-03-06 2019-05-17 康哲(湖南)制药有限公司 A kind of dedicated ultrafiltration apparatus of protein hydrolysate concentrate
CN113583861A (en) * 2021-06-24 2021-11-02 安徽铭铎生物工程有限公司 System and method for filtering biomacromolecules by using membrane coupling
CN114733353A (en) * 2022-04-14 2022-07-12 山东产研久膜科技开发有限公司 Particle size grading device and particle size grading process for superfine powder suspension

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109758912A (en) * 2019-03-06 2019-05-17 康哲(湖南)制药有限公司 A kind of dedicated ultrafiltration apparatus of protein hydrolysate concentrate
CN113583861A (en) * 2021-06-24 2021-11-02 安徽铭铎生物工程有限公司 System and method for filtering biomacromolecules by using membrane coupling
CN114733353A (en) * 2022-04-14 2022-07-12 山东产研久膜科技开发有限公司 Particle size grading device and particle size grading process for superfine powder suspension

Similar Documents

Publication Publication Date Title
US6533937B1 (en) Methods of running and washing spiral wound membrane modules
CN206566772U (en) Membrane separation device for polypeptide fractionation
EP2092974B1 (en) A method and apparatus for membrane separation applying concentration polarization
US4988445A (en) Spiral wound filtration system and method of utilizing same
EP0479492A1 (en) Membrane separation system and methods of operating and cleaning such a system
WO2002026363A3 (en) Membrane filter unit and method for filtration
CN1095964A (en) The system and method that is used for the membrane tank (casstte memberanc) of separation under the low-pressure
CN110064305A (en) A kind of device and application method improving film cycles of concentration and dope concentration
CN111266012A (en) Continuous membrane integration device and process for medicine separation and concentration
AU2010264186A1 (en) Method and plant for purifying a carbohydrate rich liquid
CN206955784U (en) Water cleaning systems
MX2007007673A (en) The separation of solid particles from the liquid in which they are dispersed.
CN108097049B (en) Integrated membrane separation and filtration method and device
KR20240038924A (en) Method for separation and purification of micro-useful substances and apparatus for separation and purification
JP3036041B2 (en) Membrane separation device
CA2347375A1 (en) Cross-flow filtration method and installation for carrying out said method
CN104785109B (en) Film group device with scrubbing mechanism and the water purifier using the film group device
CN100490951C (en) Method and apparatus for efficiently extracting or separating nano-grade matter in solution
CN103691322B (en) A kind of automatic ultrafiltration system and the hyperfiltration process of this system under constant transmembrane pressure
CN207941399U (en) A kind of Integrated Films separating and filtering device
CN212396392U (en) Optimized combination device of membrane element
CN212701358U (en) Reverse osmosis separation experiment machine
CN109264891A (en) A kind of water treatment facilities and its processing method of reverse osmosis membrane separation
CN212700871U (en) Explosion-proof multifunctional organic membrane equipment
CN218249533U (en) Multifunctional ceramic membrane testing device

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171020

Termination date: 20210111