CN206566772U - A kind of membrane separation device that separation is classified for polypeptide - Google Patents
A kind of membrane separation device that separation is classified for polypeptide Download PDFInfo
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- CN206566772U CN206566772U CN201720032151.4U CN201720032151U CN206566772U CN 206566772 U CN206566772 U CN 206566772U CN 201720032151 U CN201720032151 U CN 201720032151U CN 206566772 U CN206566772 U CN 206566772U
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Abstract
The utility model provides a kind of membrane separation device that separation is classified for polypeptide, including material storing box and classified filtering membranous system, material storing box lower end is provided with discharging opening, classified filtering membranous system includes tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component, tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component are connected in series, tubular ultrafiltration membrane module is made up of several tubular type ultra filtration chimney filters, the molecular cut off of tubular type ultra filtration chimney filter is 3000 dalton, external compression type hollow fiber ultrafiltration membrane component is made up of several hollow fibre filtering pipes, the molecular cut off of hollow fibre filtering pipe is 1000 dalton, tubular nanofiltration membrane component is made up of several tubular type nanofiltration screen pipes, the molecular cut off of tubular type nanofiltration screen pipe is 500 dalton, the utility model carries out classification separation using the membrane module of PSPP to mixed polypeptide liquid, it is big with membrane flux, separative efficiency is high, the advantages of good separating effect.
Description
Technical field
The utility model belongs to technical field of membrane separation, is filled more particularly, to a kind of UF membrane for being classified separation for polypeptide
Put.
Background technology
The peptide fragment containing different molecular weight in polypeptide, enzymolysis liquid can be made in protein after proteases for decomposing, through research
It was found that, below 3000Da polypeptide has a bioactivity, and the bioactivity that has of polypeptide also each not phase of different molecular weight
Together, therefore, it is very necessary that the polypeptide for different molecular weight, which carries out efficiently separating purification, is normally applied in the prior art
Membrane separating method is separated to the polypeptide of different molecular weight.
UF membrane is to pass through film as separating medium using selectivity, in the presence of the certain motive force in film both sides, is made in raw material
Certain compositional selecting pass through film so that mixture is separated, the purposes such as purification, concentration are reached, due to UF membrane skill
Art energy consumption is low, good separating effect the advantages of be widely used, therefore UF membrane is applied to food processing field by researcher, right
Protein enzyme solution is isolated and purified, but current film filter is mostly single-stage, it is often necessary to through a kind of membrane filtration
Afterwards, the filtering for carrying out next step after filter membrane again is changed, it is necessary to can just obtain the component of required molecular weight ranges by multiple times of filtration,
Cumbersome, efficiency is low, the purpose of series classification separation can not be played to polypeptide.
The content of the invention
Problem to be solved in the utility model be to provide a kind of simple to operate, separative efficiency it is high, being capable of series classification separation
Be used for polypeptide be classified separation membrane separation device.
In order to solve the above technical problems, the technical solution adopted in the utility model is：It is a kind of to be classified separation for polypeptide
Membrane separation device, including material storing box and classified filtering membranous system, the material storing box upper end is provided with charging aperture, the material storing box
End is provided with discharging opening, and the classified filtering membranous system is connected with the discharging opening, and the classified filtering membranous system is from left to right
Include tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component, the tubular type ultrafiltration successively
Membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane component are connected in series, the tubular ultrafiltration membrane module by
Several tubular type ultra filtration chimney filters are constituted, and the molecular cut off of the tubular type ultra filtration chimney filter is 3000 dalton, the external pressure
Formula polysulfone hollow fiber ultrafiltration membrane system is made up of several hollow fibre filtering pipes, the molecular cut off of the hollow fibre filtering pipe
For 1000 dalton, the tubular nanofiltration membrane component is made up of several tubular type nanofiltration screen pipes, the tubular type nanofiltration screen pipe
Molecular cut off be 500 dalton.
Further, the tubular ultrafiltration membrane module upper end is provided with the first inlet, the tubular ultrafiltration membrane module
End be provided with the first trapped fluid outlet, tubular ultrafiltration membrane module side lower end be provided with the first permeate outlet, it is described go out
Material mouth is connected with first inlet by the first conduit, and first switch valve, first pressure are provided with first conduit
Table and the first membrane pump.
Further, the first trapped fluid exit is provided with the first bleeder valve.
Further, the side upper end of the external compression type hollow fiber ultrafiltration membrane component is provided with the second inlet, described
The side lower end of external compression type hollow fiber ultrafiltration membrane component is provided with the outlet of the second trapped fluid, the hollow fiber ultrafiltration membrane group
Part lower end is provided with the outlet of the second permeate, and the first permeate outlet is connected with second inlet by the second conduit
Connect, second switch valve, second pressure table and the second membrane pump are provided with second conduit.
Further, the second trapped fluid exit is provided with the second bleeder valve.
Further, the upper end of the tubular nanofiltration membrane component is provided with the 3rd inlet, the tubular nanofiltration membrane component
Lower end be provided with the outlet of the 3rd trapped fluid, the side lower end of the tubular nanofiltration membrane component is provided with the outlet of the 3rd permeate,
The second permeate outlet is connected with the 3rd inlet by the 3rd conduit, and being provided with the 3rd on the 3rd conduit opens
Close valve, the 3rd pressure gauge and the 3rd membrane pump.
Further, the 3rd trapped fluid exit is provided with the 3rd bleeder valve.
Further, the 3rd permeate outlet is connected by the 4th conduit with the material storing box.
Further, it is provided with the storing chamber interior wall on liquid level sensor, the storing box outer wall and is provided with alarm
Device, the liquid level sensor is electrically connected with the warning device.
Compared with prior art, the utility model, which is had the advantage that with beneficial effect, is：
1st, the utility model uses tubular ultrafiltration membrane module, external compression type hollow fiber ultrafiltration membrane component and tubular nanofiltration membrane group
The mode that part is serially connected, according to the difference of membrane module molecular cut off, is separated, together step by step to the polypeptide of different molecular weight
Shi Yingyong tubular membranes and external pressing type hollow fibrous membrane are as main membrane module form, with film surface area is big, flux is big, operation
The advantages of pressure difference is relatively low, and integral device is simple in construction, good separating effect；
The 2nd, liquid level sensor is set in material storing box, while liquid level sensor is electrically connected with volume warning device on storing box outer wall
Connect, when liquid level does not reach the level value of liquid level sensor setting in material storing box, control warning device alarm can be reminded in time
Level condition in material storing box, it is safe.
Brief description of the drawings
Fig. 1 is a kind of structural representation for the membrane separation device that separation is classified for polypeptide of the utility model.
In figure：1- material storing box；2- charging apertures；3- discharging openings；4- tubular ultrafiltration membrane modules；5- external-compression type Hollow Fiber Ultrafiltrations
Membrane module；6- tubular nanofiltration membrane components；7- tubular type ultra filtration chimney filters；8- hollow fibre filtering pipes；9- tubular type nanofiltration screen pipes；
The inlets of 10- first；The trapped fluids of 11- first are exported；The permeate of 12- first is exported；13- first switch valves；14- first pressures
Table；The membrane pumps of 15- first；The bleeder valves of 16- first；The conduits of 17- first；The inlets of 18- second；The trapped fluids of 19- second are exported；
The permeate of 20- second is exported；The conduits of 21- second；22- second switch valves；23- second pressure tables；The membrane pumps of 24- second；25-
Two bleeder valves；The inlets of 26- the 3rd；The trapped fluids of 27- the 3rd are exported；The permeate of 28- the 3rd is exported；The conduits of 29- the 3rd；30-
Three switch valves；The pressure gauges of 31- the 3rd；The membrane pumps of 32- the 3rd；The bleeder valves of 33- the 3rd；The conduits of 34- the 4th；35- liquid level sensors；
36- warning devices.
Embodiment
Embodiment of the present utility model is elaborated below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of membrane separation device that separation is classified for polypeptide, including material storing box 1 and classified filtering membrane system
System, the upper end of material storing box 1 is provided with charging aperture 2, and the lower end of material storing box 1 is provided with discharging opening 3, classified filtering membranous system and discharging opening 3
Connection, classified filtering membranous system includes tubular ultrafiltration membrane module 4, external compression type hollow fiber ultrafiltration membrane component 5 successively from left to right
With tubular nanofiltration membrane component 6, tubular ultrafiltration membrane module 4, external compression type hollow fiber ultrafiltration membrane component 5 and tubular nanofiltration membrane component 6
It is connected in series, the upper end of tubular ultrafiltration membrane module 4 is provided with the first inlet 10, the lower end of tubular ultrafiltration membrane module 4 is provided with first
Trapped fluid outlet 11, the side lower end of tubular ultrafiltration membrane module 4 is provided with the first permeate outlet 12, the feed liquor of discharging opening 3 and first
Mouth 10 is connected by the first conduit 17, and first switch valve 13, the barrier film of first pressure table 14 and first are provided with the first conduit 17
Pump 15, the side upper end of external compression type hollow fiber ultrafiltration membrane component 5 is provided with the second inlet 18, external-compression type Hollow Fiber Ultrafiltration
The side lower end of membrane module 5 is provided with the second trapped fluid outlet 19, and the lower end of polysulfone hollow fiber ultrafiltration membrane system 5 is provided with the second transmission
Liquid outlet 20, the first permeate outlet 12 is connected with the second inlet 18 by the second conduit 21, is provided with the second conduit 21
Second switch valve 22, the membrane pump 24 of second pressure table 23 and second, the upper end of tubular nanofiltration membrane component 6 is provided with the 3rd inlet
26, the lower end of tubular nanofiltration membrane component 6 is provided with the 3rd trapped fluid outlet 27, and the side lower end of tubular nanofiltration membrane component 6 is set
There is the 3rd permeate to export 28, the second permeate outlet 20 is connected with the 3rd inlet 26 by the 3rd conduit 29, the 3rd conduit
The 3rd switch valve 30, the 3rd pressure gauge 31 and the 3rd membrane pump 32 are provided with 29, the 3rd permeate outlet 28 is led by the 4th
Pipe 34 is connected with material storing box 1, circulation loop is formed between material storing box 1 and classified filtering membranous system, polypeptide mixed liquor is through undue
During level filtration membrane systems, what is flowed out in trapped fluid exit is the polypeptide solution of required molecular weight.
Wherein, tubular ultrafiltration membrane module 4 is made up of several tubular type ultra filtration chimney filters 7, and tubular type ultra filtration chimney filter 7 can be
The hydrophilic film of the materials such as makrolon, Kynoar or polyamide, the molecular cut off of tubular type ultra filtration chimney filter 7 is 3000
Dalton, external compression type hollow fiber ultrafiltration membrane component 5 is made up of several hollow fibre filtering pipes 8, and hollow fibre filtering pipe 8 can
Think the hydrophilic film of the materials such as makrolon, Kynoar, polyamide or polyacrylonitrile, section of hollow fibre filtering pipe 8
It is 1000 dalton to stay molecular weight, and tubular nanofiltration membrane component 6 is made up of several tubular type nanofiltration screen pipes 9, tubular type nanofiltration filtering
Pipe 9 can be aromatic polyamides composite membrane material or zirconium dioxide-PEI organic-inorganic nanofiltration membrane material, tubular type nanofiltration
The molecular cut off of screen pipe 9 is 500 dalton, can prevent to form moisture film on film surface using hydrophilic film material, it is ensured that
The stability of membrane flux, improves filter efficiency, and mixtures of polypeptides is effectively divided difference by the membrane module of PSPP
The peptide separation of son amount comes out, good separating effect.
The course of work of the present utility model is：Polypeptide mixed liquor is placed into material storing box 1 first, first switch valve is opened
13, start the first membrane pump 15, polypeptide mixed liquor is pumped into tubular ultrafiltration membrane module 4, control first switch valve 13, control
The filter pressure of tubular membrane component 4 is between 0.2MPa-0.7MPa, because the molecular cut off of tubular ultrafiltration membrane module 4 is
3000 dalton, can will be greater than the polypeptide retention of 3000 Dalton molecular weights, be collected at the first trapped fluid outlet 11,
The polypeptide that molecular weight is less than 3000 dalton enters external-compression type by tubular ultrafiltration membrane module 4 by the first permeate outlet 12
In hollow fiber film assembly 5, by controlling the unlatching size of second switch valve 22 to control the mistake of external pressure type hollow fiber membrane component 5
Filtering pressure power is between 0.5MPa-0.8MPa, and the molecular cut off of external pressure type hollow fiber membrane component 5 is 1000 dalton, therefore
Peptide molecule more than 1000 dalton is trapped, and is collected at the second trapped fluid outlet 19, permeate is saturating by second
Cross liquid outlet 19 to enter in tubular nanofiltration membrane component 6, control the 3rd switch valve 30, tubular type nanofiltration membrane component 6 is controlled with this
Filter pressure is between 0.1MPa-0.3MPa, and the molecular cut off of tubular nanofiltration membrane component 6 is 500 dalton, therefore is more than
The peptide molecule of 500 dalton is trapped, and is collected at the 3rd trapped fluid outlet 27, permeate passes through the 3rd permeate 27
Outlet is come back in material storing box 1, and the liquid level sensor 35 carried out again in circulation classification separation process, material storing box 1 is detected
When liquid level in material storing box 1 is less than setting value, control warning device 36 is alarmed, it is ensured that the security of the equipment course of work.
One embodiment of the present utility model is described in detail above, but the content is only of the present utility model
Preferred embodiment, it is impossible to be considered as being used to limit practical range of the present utility model.It is all to be made according to present utility model application scope
Equivalent change with improve etc., all should still belong within patent covering scope of the present utility model.
Claims (9)
1. a kind of membrane separation device that separation is classified for polypeptide, including material storing box (1) and classified filtering membranous system, the storing
Case (1) upper end is provided with charging aperture (2), and material storing box (1) lower end is provided with discharging opening (3), the classified filtering membranous system
It is connected with the discharging opening (3), it is characterised in that：The classified filtering membranous system includes tubular ultra-filtration membrane group successively from left to right
Part (4), external compression type hollow fiber ultrafiltration membrane component (5) and tubular nanofiltration membrane component (6), it is the tubular ultrafiltration membrane module (4), outer
Pressure type hollow fiber hyperfiltration membrane assembly (5) and tubular nanofiltration membrane component (6) are connected in series, the tubular ultrafiltration membrane module (4) by
Several tubular type ultra filtration chimney filters (7) are constituted, and the molecular cut off of the tubular type ultra filtration chimney filter (7) is 3000 dalton, institute
State external compression type hollow fiber ultrafiltration membrane component (5) to be made up of several hollow fibre filtering pipes (8), the hollow fibre filtering pipe
(8) molecular cut off is 1000 dalton, and the tubular nanofiltration membrane component (6) is by several tubular type nanofiltration screen pipe (9) groups
Into the molecular cut off of the tubular type nanofiltration screen pipe (9) is 500 dalton.
2. a kind of membrane separation device that separation is classified for polypeptide according to claim 1, it is characterised in that：The tubular type
Hyperfiltration membrane assembly (4) upper end is provided with the first inlet (10), and tubular ultrafiltration membrane module (4) lower end is provided with the first retention
Liquid exports (11), and tubular ultrafiltration membrane module (4) the side lower end is provided with the first permeate outlet (12), the discharging opening
(3) it is connected with first inlet (10) by the first conduit (17), first switch is provided with first conduit (17)
Valve (13), first pressure table (14) and the first membrane pump (15).
3. a kind of membrane separation device that separation is classified for polypeptide according to claim 2, it is characterised in that：Described first
Trapped fluid outlet (11) place is provided with the first bleeder valve (16).
4. a kind of membrane separation device that separation is classified for polypeptide according to claim 2, it is characterised in that：The external pressure
The side upper end of formula polysulfone hollow fiber ultrafiltration membrane system (5) is provided with the second inlet (18), the external-compression type Hollow Fiber Ultrafiltration
The side lower end of membrane module (5) is provided with the second trapped fluid outlet (19), and polysulfone hollow fiber ultrafiltration membrane system (5) lower end is set
There is the second permeate to export (20), the first permeate outlet (12) passes through the second conduit with second inlet (18)
(21) connect, second switch valve (22), second pressure table (23) and the second membrane pump are provided with second conduit (21)
(24)。
5. a kind of membrane separation device that separation is classified for polypeptide according to claim 4, it is characterised in that：Described second
Trapped fluid outlet (19) place is provided with the second bleeder valve (25).
6. a kind of membrane separation device that separation is classified for polypeptide according to claim 4, it is characterised in that：The tubular type
The upper end of nanofiltration membrane component (6) is provided with the 3rd inlet (26), and the lower end of the tubular nanofiltration membrane component (6) is provided with the 3rd
Trapped fluid exports (27), and the side lower end of the tubular nanofiltration membrane component (6) is provided with the 3rd permeate outlet (28), and described the
Two permeate outlet (20) is connected with the 3rd inlet (26) by the 3rd conduit (29), is set on the 3rd conduit (29)
It is equipped with the 3rd switch valve (30), the 3rd pressure gauge (31) and the 3rd membrane pump (32).
7. a kind of membrane separation device that separation is classified for polypeptide according to claim 6, it is characterised in that：Described 3rd
Trapped fluid outlet (27) place is provided with the 3rd bleeder valve (33).
8. a kind of membrane separation device that separation is classified for polypeptide according to claim 6, it is characterised in that：Described 3rd
Permeate outlet (28) is connected by the 4th conduit (34) with the material storing box (1).
9. a kind of membrane separation device that separation is classified for polypeptide according to claim 1, it is characterised in that：The storing
It is provided with case (1) inwall on liquid level sensor (35), material storing box (1) outer wall and is provided with warning device (36), the liquid
Level sensor (35) is electrically connected with the warning device (36).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720032151.4U CN206566772U (en) | 2017-01-11 | 2017-01-11 | A kind of membrane separation device that separation is classified for polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720032151.4U CN206566772U (en) | 2017-01-11 | 2017-01-11 | A kind of membrane separation device that separation is classified for polypeptide |
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| Publication Number | Publication Date |
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| CN206566772U true CN206566772U (en) | 2017-10-20 |
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| CN201720032151.4U Expired - Fee Related CN206566772U (en) | 2017-01-11 | 2017-01-11 | A kind of membrane separation device that separation is classified for polypeptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758912A (en) * | 2019-03-06 | 2019-05-17 | 康哲(湖南)制药有限公司 | A kind of dedicated ultrafiltration apparatus of protein hydrolysate concentrate |
| CN113583861A (en) * | 2021-06-24 | 2021-11-02 | 安徽铭铎生物工程有限公司 | System and method for filtering biomacromolecules by using membrane coupling |
| CN114733353A (en) * | 2022-04-14 | 2022-07-12 | 山东产研久膜科技开发有限公司 | Particle size grading device and particle size grading process for superfine powder suspension |
-
2017
- 2017-01-11 CN CN201720032151.4U patent/CN206566772U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758912A (en) * | 2019-03-06 | 2019-05-17 | 康哲(湖南)制药有限公司 | A kind of dedicated ultrafiltration apparatus of protein hydrolysate concentrate |
| CN113583861A (en) * | 2021-06-24 | 2021-11-02 | 安徽铭铎生物工程有限公司 | System and method for filtering biomacromolecules by using membrane coupling |
| CN114733353A (en) * | 2022-04-14 | 2022-07-12 | 山东产研久膜科技开发有限公司 | Particle size grading device and particle size grading process for superfine powder suspension |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171020 Termination date: 20210111 |
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| CF01 | Termination of patent right due to non-payment of annual fee |