Utility model content
The purpose of this utility model is that providing organophosphorus pesticide residual fast examines case, makes each several part gap in inner surface and case
Preset foam-rubber cushion and used for filler wall in damping and anti-extrusion.The utility model compact, is easy to carry, and internal structure design is complete
Face rationally, power supply can be departed from short term and laboratory is relied on, the quick detection for scene.
In order to reach above-mentioned purpose, the utility model employs following technical scheme, organophosphorus pesticide quick testing kit, bag
Casing and upper lid are included, the box house is provided with extensible biological ice box rest area, the box-packed suction nozzle of 200ul pipettors and placed
Area, the box-packed suction nozzle rest area of 5ml pipettors, portable rechargeable-type instrument for detecting pesticide residue through rest area, 500ml buffering agents bottle are put
Putting area, wide-mouth waste liquid bottle rest area, wash bottle rest area, box-packed cuvette rest area, 5ml adjustable pipettes rest area, 200ul can
Adjust pipettor rest area and auxiliary rest area.
Extensible biological ice box is placed with the extensible biological ice box rest area, the 200ul pipettors are box-packed to be inhaled
Head rest area is placed with the box-packed suction nozzle of 200ul pipettors, and the box-packed suction nozzle rest area of 5ml pipettors is placed with 5ml pipettors
Box-packed suction nozzle, the portable rechargeable-type instrument for detecting pesticide residue through rest area is placed with portable rechargeable-type instrument for detecting pesticide residue through, institute
State 500ml buffering agents bottle rest area and be placed with 500ml buffering agents bottle, the wide-mouth waste liquid bottle rest area is placed with extensively
Mouth waste liquid bottle, the wash bottle rest area is placed with wash bottle, and the box-packed cuvette rest area is placed with box-packed cuvette, described
5ml adjustable pipettes rest area is placed with 5ml adjustable pipettes, and the 200ul adjustable pipettes rest area is placed with 200ul can
Adjust pipettor.
Agent for storage of coldness interlayer built in the extensible biological ice box, 4 DEG C or so of storage temperature that Keep cool reserves 6 examinations
Agent hole position.
The auxiliary rest area, in extensible biological ice box rest area, the box-packed suction nozzle rest area of 200ul pipettors, box-packed
Cuvette rest area, 5ml adjustable pipettes rest area, space segment is used as auxiliary above 200ul adjustable pipettes rest area
Rest area.
The auxiliary rest area be used for place buffer solution pulvis, micro balance, specimen cup, reaction tube, small suction pipe, filter paper,
Mouth mask, gloves, scissors, tweezers, timer, Marker, minute book.
The outside on the casing and upper lid long side below sets 2 hinge conjoined devices, long before the casing and upper lid
2 shutoff devices are set on the outside of side, and upper cap upper surface centre sets one piece of lift-pull handle.
The box inner structure is pre-designed sponge buffering mold shape, i.e., each rest area is formed and each by mould punching
Has sponge cushion in rest area.
Compared to prior art, the utility model has the advantages that:
In the utility model case inner surface and case each several part gap according to internal structure preset foam-rubber cushion and it is used for filler wall in
Damping and anti-extrusion.Internal structure design rationally, various required objects can be placed to greatest extent.In casing and the long side of upper lid
Outside in the middle of both sides set 2 hinge conjoined devices facilitate casing folding, to set laterally 2 shutoff devices prevent transport
Middle casing is opened, and upper outer side of lid centre sets one piece of lift-pull handle.Compact, is easy to carry, can depart from short term power supply and
Laboratory is relied on, the quick detection for scene.The organophosphorus pesticide quick testing kit is used for the food such as quick detection vegetable and fruit
Product Pesticide Residues, according to the GB standard of enzyme inhibition rate method in GB/T 5009.199-2003, are equipped with used in detection process
All devices, reagent and the consumptive material arrived, experiment detection can be completed without being equipped with other any materials by the operating process of national standard.
Its operation principle is:Organophosphorus pesticide occurs after suppressing the activity of cholinesterase, and then inhibitory enzyme decomposition substrate with developer
The degree of chromogenic reaction, can obtain final suppression rate score in particular detection equipment.
Embodiment
Relevant detailed description of the present utility model and technology contents, coordinate brief description of the drawings as follows, but accompanying drawing is only reference
With purposes of discussion, not for being any limitation as to the utility model.
As Figure 1-3, organophosphorus pesticide quick testing kit, including casing and upper lid, the box house is provided with can
Remove biological ice box rest area 1, it is the box-packed suction nozzle rest area 2 of 200ul pipettors, the box-packed suction nozzle rest area 3 of 5ml pipettors, portable
Rechargeable instrument for detecting pesticide residue through rest area 4,500ml buffering agents bottle rest area 5, wide-mouth waste liquid bottle rest area 6, wash bottle are put
Put area 7, box-packed cuvette rest area 8,5ml adjustable pipettes rest area 9,200ul adjustable pipettes rest area 10, sponge buffering
Pad and filling wall 11.
Extensible biological ice box is placed with the extensible biological ice box rest area, the 200ul pipettors are box-packed to be inhaled
Head rest area is placed with the box-packed suction nozzle of 200ul pipettors, and the box-packed suction nozzle rest area of 5ml pipettors is placed with 5ml pipettors
Box-packed suction nozzle, the portable rechargeable-type instrument for detecting pesticide residue through rest area is placed with portable rechargeable-type instrument for detecting pesticide residue through, institute
State 500ml buffering agents bottle rest area and be placed with 500ml buffering agents bottle, the wide-mouth waste liquid bottle rest area is placed with extensively
Mouth waste liquid bottle, the wash bottle rest area is placed with wash bottle, and the box-packed cuvette rest area is placed with box-packed cuvette, described
5ml adjustable pipettes rest area is placed with 5ml adjustable pipettes, and the 200ul adjustable pipettes rest area is placed with 200ul can
Adjust pipettor.
1) extensible biological ice box rest area:Built-in agent for storage of coldness interlayer, 2~8 DEG C or so of storage temperature that Keep cool, in advance
6 reagent hole positions are stayed, can be needed to place enzyme, substrate and the bottled reagent of developer according to experiment.
2) the box-packed suction nozzle rest area of 200ul pipettors;Ul for microlitre, volume unit;
3) the box-packed suction nozzle rest area of 5ml pipettors;Ml is milliliter, volume unit;
4) portable rechargeable-type instrument for detecting pesticide residue through (containing charging wire) rest area;
5) 500ml buffering agents bottle rest area, can prepare and hold buffer solution component;
6) wide-mouth waste liquid bottle (containing closure) rest area;
7) wash bottle rest area;
8) box-packed cuvette rest area;
9) 5ml adjustable pipettes rest area;
10) 200ul adjustable pipettes rest area;
11) sponge cushion pad and filling wall
Also aid in rest area;In extensible biological ice box rest area 1, the box-packed suction nozzle rest area 2 of 200ul pipettors, box
Fill space above the regions such as cuvette rest area 8,5ml adjustable pipettes rest area 9,200ul adjustable pipettes rest area 10
Part is as auxiliary rest area, and can fill a vacancy placement buffer solution pulvis, micro balance, specimen cup, reaction tube, small suction pipe, filter paper, mouth
Cover, gloves, scissors, tweezers, timer, Marker, the part such as minute book.
Casing outward appearance is aluminum, and inwall is coated with spongy cushion pad, long 42cm, wide 28cm, high 20cm (bottom casing depths
16cm, upper lid depth 4cm), both sides set 2 hinge conjoined devices to facilitate casing to open in the middle of the outside on casing and the long side of upper lid
Close, to setting 2 shutoff devices to prevent that casing is opened in transport laterally, upper outer side of lid centre sets one piece of lift-pull handle.
Quick testing kit internal structure:Each several part gap is pre-designed sponge cushion pad according to internal structure in advance in case and sponge fills wall,
I.e. each rest area is formed by mould punching and each rest area has sponge cushion.
Operating procedure:1. opening quick testing kit, take out enzyme, developer and substrate reagent within 5 minutes in advance and rise again.
2. choosing representational fruits and vegetables removes soil, weigh in the balance and take 2g, leaf vegetables takes blade-section, melon and fruit takes skin 4g.
3. dish sample is cut into 1cm sizes to be placed in specimen cup, 10mL buffer solutions, mechanical shaking extraction 3 minutes are added.
, should be first if sample liquid excessively muddy or chlorophyll is excessive 4. after clarification 2 minutes, supernatant is sample extraction liquid
Drawn again after filtering.
Preheated 5. instrument is first started shooting 10 minutes.
6. blank control:2.5ml buffer solutions, 100ul developers, 100ul enzymes, in palm are added in reaction tube successively
Insulation 15 minutes, after add 100ul substrates, pour into cuvette rapidly, be put into Instrument measuring.
7. pattern detection:2.5ml sample extraction liquid replaces buffer solution, the same blank control of other steps.
Result judgement:1. inhibiting rate<To be qualified when 50%;Residues of pesticides are exceeded during suppression Shuai≤50%.
2. advise carrying out diplopore repetition measurement to result for 45-55% sample.
Points for attention:In green onion, garlic, radish, leek, celery, caraway, wild rice stem, mushroom and tomato tissue liquid, containing having to enzyme
The plant secondary substance of influence, easily produces false positive.When handling this kind of sample, whole strain (body) vegetables can be taken to extract.To one
A bit containing the higher vegetables of chlorophyll, the method that can also take whole strain (body) vegetables to extract reduces the interference of pigment.
Preferred embodiment of the present utility model is the foregoing is only, is not used to limit the scope of the claims of the present utility model, its
He uses the equivalence changes of the utility model patent spirit, all should all belong to the scope of the claims of the present utility model.