CN206531811U - Instrument for detecting pesticide residue through - Google Patents
Instrument for detecting pesticide residue through Download PDFInfo
- Publication number
- CN206531811U CN206531811U CN201621408772.XU CN201621408772U CN206531811U CN 206531811 U CN206531811 U CN 206531811U CN 201621408772 U CN201621408772 U CN 201621408772U CN 206531811 U CN206531811 U CN 206531811U
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- China
- Prior art keywords
- circuit
- instrument
- pesticide residue
- detecting pesticide
- enzyme electrode
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Instrument for detecting pesticide residue through, belongs to detecting instrument field.Traditional instrument for detecting pesticide residue through is developed using enzyme level principle and photoelectric colorimetry principle.Have the disadvantage that use occasion is limited, when being detected on the spot, its volume is heavy, operation requires height, it is necessary to skilled addressee's cooperating, it is impossible to meet the requirement of field quick detection.The utility model includes enzyme electrode sensor, one end connection potentiostat of enzyme electrode sensor, the other end connection current-to-voltage converting circuit of enzyme electrode sensor, current-to-voltage converting circuit connects second amplifying circuit, second amplifying circuit connects two-stage low-pass wave circuit, two-stage low-pass wave circuit connects A/D converter circuit, A/D converter circuit connects single chip machine controlling circuit, single chip machine controlling circuit connection LCDs, printer, button, power supply, temperature sensor, temperature sensor connection heater.The utility model can accurately be realized and garden stuff pesticide residue amount is quantitatively detected.
Description
Technical field
The utility model belongs to detecting instrument field；Specifically related to a kind of instrument for detecting pesticide residue through.
Background technology
Residues of pesticides are the agricultural chemicals being applied on crop, and a portion is attached on crop, a part be scattered in soil,
In the environment such as air and water, the part in the agricultural chemicals of environment remaining can be absorbed by plants again.Remains of pesticide directly passes through plant
Fruit or water, air are reached in people, animal body, or are ultimately transferred to people, animal by environment, food chain.Human body daily all roots from food
The need for the element that absorbed nourishment in thing meets body, human body is recommended to need 1 jin or so of vegetable and fruit, vegetable according to Chinese Soclety of Nutrition
Dish fruit is ascorbic main source, and the type of the daily remains of pesticide of human body is mainly organic phosphates and organochlorine class.It is edible
A small amount of remains of pesticide, human body itself can be degraded, and acute poisoning will not be caused suddenly, but long-term consumption is not cleaned up and carried
The agricultural product of remains of pesticide, will necessarily bring great harm to health.
Traditional instrument for detecting pesticide residue through is developed using enzyme level principle and photoelectric colorimetry principle.In certain condition
Under, organophosphor and carbamic acid class agricultural chemicals have inhibitory action to cholinesterase normal function, and the concentration of its inhibiting rate and agricultural chemicals is in
Positive correlation.Under normal circumstances, enzymatic nerve conduction metabolite (acetylcholine) is hydrolyzed, and its hydrolysate and developer are anti-
Should, yellow substance is produced, changed with time value with absorbance under spectrophotometric determination 412nm, is calculated inhibiting rate, is passed through
Inhibiting rate may determine that in sample whether the residual containing organophosphor or carbamate chemicals for agriculture.Can realize organophosphor and
The field quick detection of carbamate pesticide residue amount, advantage is that test limit is low, precision is high, sensitivity is also stronger；Lack
Point is that use occasion is limited, when being detected on the spot, and its volume is heavy, operation requires high, it is necessary to which skilled addressee cooperates with
Operation, it is impossible to meet the requirement of field quick detection.
The content of the invention
The utility model purpose is to provide a kind of instrument for detecting pesticide residue through of current enzyme electrode biology sensor, simplifies
Conventional detection process, can accurately realize and garden stuff pesticide residue amount is quantitatively detected, and simple to operate, practical.
Above-mentioned purpose is achieved through the following technical solutions：
A kind of instrument for detecting pesticide residue through, including enzyme electrode sensor, one end connection of described enzyme electrode sensor are permanent electric
Position instrument, the other end of described enzyme electrode sensor connects current-to-voltage converting circuit, and described current-to-voltage converting circuit connects
Connect second amplifying circuit, described second amplifying circuit connection two-stage low-pass wave circuit, described two-stage low-pass wave circuit
Connect A/D converter circuit, described A/D converter circuit connection single chip machine controlling circuit, described single chip machine controlling circuit connection liquid
Crystal display screen, printer, button, power supply, temperature sensor, described temperature sensor connection heater.
Instrument for detecting pesticide residue through described in the utility model, described potentiostat current potential output voltage for 100~
1000mV.When providing 1V operating voltage to enzyme electrode, under the electrode potential phase occurs for the p-nitrophenol in prepare liquid
To irreversible redox reaction, low current signal to be detected is thus produced by detecting that circuit carries out electricity to the signal
Flow voltage conversion, two grades of amplifications and LPF, can voltage of the output area between 0~5V to A/D converter.
Operational amplifier in instrument for detecting pesticide residue through described in the utility model, described current-to-voltage converting circuit is
OP07 low noise bipolar operational amplifiers, its Differential Input resistance is 33M Ω, and bias current is 1.8nA, and gain representative value is
104dB, common-mode rejection ratio 110dB, with low-down input offset voltage, are 25 μ V to the maximum.
Operational amplifier in instrument for detecting pesticide residue through described in the utility model, described second amplifying circuit is
AD622 operational amplification circuits；By changing RV resistance, the multiple of instrument amplifier is adjusted, changes the size of output voltage, most
The voltage of 0~5V scopes is obtained afterwards.
Instrument for detecting pesticide residue through described in the utility model, described two-stage low-pass wave circuit is active circuit, is passed through
Introduce appropriate positive feedback so that filter circuit has certain amplification, and does not produce self-oscillation and cause circuit unstable
It is fixed.
Conversion chip in instrument for detecting pesticide residue through described in the utility model, described A/D converter circuit is 16 A/D
Conversion chip AD7705, the programmable gain amplifier of the built-in chip type, digital filter and calibration option, improves system
Accuracy of detection.
Single-chip microcomputer model in instrument for detecting pesticide residue through described in the utility model, described single chip machine controlling circuit is
STC89C52.In the utility model enzyme electrode and the reacted micro-current signal of prepare liquid, circuit amplifies after testing, after filtering, obtains
To voltage signal through over-sampling deliver to single-chip microcomputer processing, finally shown in the form of organophosphorus pesticide concentration in prepare liquid
Come.Described single chip machine controlling circuit control LCDs, printer, button, power supply, temperature sensor and heater.
Pesticides Testing sample is added dropwise on instrument for detecting pesticide residue through described in the utility model, described enzyme electrode sensor.
Hydrolase electrode test uniformity is good, and testing result has repeatability；Pass through current-to-voltage converting circuit, secondary amplification circuit
And low-pass filter circuit, the micro-current signal between zero to hundreds of nas that enzyme electrode reaction is obtained can be extracted well；Simplify
Conventional detection process, can accurately realize garden stuff pesticide residue amount is quantitatively detected, precision is high, and simple to operate,
It is practical.
Brief description of the drawings
Fig. 1 is System Working Principle block diagram of the present utility model；
Fig. 2 is current-to-voltage converting circuit figure of the present utility model；
Fig. 3 is second amplifying circuit figure of the present utility model；
Fig. 4 is two-stage low-pass wave circuit figure of the present utility model；
Fig. 5 is A/D converter circuit of the present utility model；
Fig. 6 is single chip machine controlling circuit figure of the present utility model.
Embodiment
Embodiment one：
A kind of instrument for detecting pesticide residue through, including enzyme electrode sensor 1, one end connection of described enzyme electrode sensor are permanent electric
Position instrument 2, the other end of described enzyme electrode sensor connects current-to-voltage converting circuit 4, described current-to-voltage converting circuit
Connect second amplifying circuit 5, described second amplifying circuit connection two-stage low-pass wave circuit 6, described two-stage low-pass ripple
Circuit connects A/D converter circuit 7, described A/D converter circuit connection single chip machine controlling circuit 8, described single chip machine controlling circuit
Connect LCDs 9, printer 10, button 11, power supply 12, temperature sensor 13, described temperature sensor connection heating
Device 14.
Instrument for detecting pesticide residue through described in present embodiment, the output voltage of described potentiostat is 100~1000mV；
Operational amplifier in described current-to-voltage converting circuit is OP07 low noise bipolar operational amplifiers；Described two grades are put
Operational amplifier in big circuit is AD622 operational amplification circuits；Described two-stage low-pass wave circuit is active circuit；It is described
A/D converter circuit in conversion chip be 16 A/D conversion chips AD7705；Monolithic in described single chip machine controlling circuit
Type number is STC89C52.
Pesticides Testing sample is added dropwise on instrument for detecting pesticide residue through described in present embodiment, described enzyme electrode sensor.
Embodiment two：
Instrument for detecting pesticide residue through according to embodiment one, described current-to-voltage converting circuit Rf feedback resistances resistance
It is worth for 100k Ω, Cf feedback capacities value is 100nF, R3 provides a suitable quiescent biasing for the transistor of chip internal,
The balance resistance of zero point error is controlled, adjustable resistance is used for giving OP07 zeroings, output during zero input is also mutually should be zero.
Embodiment three：
Instrument for detecting pesticide residue through according to embodiment one, described two-stage low-pass wave circuit filter circuit cut-off
Frequency fP=50Hz, most suitable quality factor q=0.7, C1=C2=1 μ F, the R1=R2=R3=R4=10K Ω of filter circuit.
Embodiment four：
Instrument for detecting pesticide residue through according to embodiment one, described heater can heat testing liquid, keep
Solution to be measured is detected at a constant temperature, reduces measurement error.
Claims (7)
1. a kind of instrument for detecting pesticide residue through, it is characterised in that：Including enzyme electrode sensor (1), described enzyme electrode sensor (1)
One end connection potentiostat (2), the other end connection current-to-voltage converting circuit (4) of described enzyme electrode sensor (1), institute
Current-to-voltage converting circuit (4) the connection second amplifying circuit (5) stated, described second amplifying circuit (5) connects two grades of low passes
Filter circuit (6), described two-stage low-pass wave circuit (6) connection A/D converter circuit (7), described A/D converter circuit (7) is even
Single chip machine controlling circuit (8) is connect, described single chip machine controlling circuit (8) connects LCDs (9), printer (10), button
(11), power supply (12), temperature sensor (13), described temperature sensor (13) connection heater (14)；Described two grades are put
Operational amplifier in big circuit (5) is AD622 operational amplification circuits；Described current-to-voltage converting circuit (4) Rf feedback electricity
It is that 100k Ω, Cf feedback capacities value is 100nF to hinder resistance；Described two-stage low-pass wave circuit (6) filter circuit cutoff frequency
Rate fP=50Hz, most suitable quality factor q=0.7, C1=C2=1 μ F, the R1=R2=R3=R4=10K Ω of filter circuit.
2. instrument for detecting pesticide residue through according to claim 1, it is characterised in that：The output electricity of described potentiostat (2)
Press as 100~1000mV.
3. instrument for detecting pesticide residue through according to claim 1 or 2, it is characterised in that：Described current-to-voltage converting circuit
(4) operational amplifier in is OP07 low noise bipolar operational amplifiers.
4. instrument for detecting pesticide residue through according to claim 1 or 2, it is characterised in that：Described two-stage low-pass wave circuit
(6) it is active circuit.
5. instrument for detecting pesticide residue through according to claim 1 or 2, it is characterised in that：In described A/D converter circuit (7)
Conversion chip is 16 A/D conversion chips AD7705.
6. instrument for detecting pesticide residue through according to claim 1 or 2, it is characterised in that：Described single chip machine controlling circuit (8)
In single-chip microcomputer model be STC89C52.
7. instrument for detecting pesticide residue through according to claim 1 or 2, it is characterised in that：On described enzyme electrode sensor (1)
Pesticides Testing sample is added dropwise.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621408772.XU CN206531811U (en) | 2016-12-21 | 2016-12-21 | Instrument for detecting pesticide residue through |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621408772.XU CN206531811U (en) | 2016-12-21 | 2016-12-21 | Instrument for detecting pesticide residue through |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206531811U true CN206531811U (en) | 2017-09-29 |
Family
ID=59922311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621408772.XU Expired - Fee Related CN206531811U (en) | 2016-12-21 | 2016-12-21 | Instrument for detecting pesticide residue through |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206531811U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108709967A (en) * | 2018-06-20 | 2018-10-26 | 何伟建 | A kind of detection device for Watermelon Maturity and pesticide residue |
| CN110346439A (en) * | 2019-08-01 | 2019-10-18 | 昆明学院 | A kind of Pesticide-Polluted Soil detection device |
-
2016
- 2016-12-21 CN CN201621408772.XU patent/CN206531811U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108709967A (en) * | 2018-06-20 | 2018-10-26 | 何伟建 | A kind of detection device for Watermelon Maturity and pesticide residue |
| CN110346439A (en) * | 2019-08-01 | 2019-10-18 | 昆明学院 | A kind of Pesticide-Polluted Soil detection device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170929 Termination date: 20171221 |
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| CF01 | Termination of patent right due to non-payment of annual fee |