CN206529464U - A kind of fermentation tank being separately cultured for resin original position - Google Patents

A kind of fermentation tank being separately cultured for resin original position Download PDF

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Publication number
CN206529464U
CN206529464U CN201720209393.6U CN201720209393U CN206529464U CN 206529464 U CN206529464 U CN 206529464U CN 201720209393 U CN201720209393 U CN 201720209393U CN 206529464 U CN206529464 U CN 206529464U
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Prior art keywords
tank
fermentation tank
separately cultured
resin
original position
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CN201720209393.6U
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Chinese (zh)
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张鹏
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Shanghai Longyin Biotechnology Co., Ltd.
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Burton (shanghai) Biotechnology Co Ltd
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Abstract

The utility model discloses a kind of fermentation tank being separately cultured for resin original position, including fermentation tank, material inlet is provided with the top of tank body, motor is provided with outside tank body, motor is connected with the axis of centres, and tank inner chamber is extended to outside tank by axle envelope, agitator is provided with the axis of centres, the bottom of fermentation tank is provided with tank base, tank base is provided with the discharging opening of resin particle, fermentation tank tank base side wall is provided with the leakage fluid dram of a different height position simultaneously, flushing liquor entrance is provided with the top of fermentation tank, flushing liquor entrance is connected with shower nozzle, the fermentation tank can fast and easy be separately cultured liquid and resin, avoid the problem of resin particle blocking pipeline or valve occur for blowing stage or separation phase.Resin is directly reclaimed after the Zymolysis Equipment can be separately cultured in the original location simultaneously, so that the pipeline stage avoided expends substantial amounts of conveying liquid, the follow-up step treatment fluid scale of construction is reduced.

Description

A kind of fermentation tank being separately cultured for resin original position
Technical field
The utility model is related to a kind of fermentation tank, and in particular to a kind of fermentation tank being separately cultured for resin original position, Belong to biological fermentation field.
Background technology
It is to be usually used in microorganism, cell culture of animals fermentation technique releasing in incubation that original position, which is separately cultured technology, One of the technology of Product inhibiton.It refers in incubation, while carry out product separation or absorption, by product from culture medium In separate to reduce Product inhibiton or the toxicity to cell, so as to increase a kind of mode of Product yields.Resin original position point Then refer to while the fermentation of fermentation tank from culture, addition resin particle in direct culture tank, in separation absorption culture medium Product, so as to reduce a kind of mode that Product inhibiton improves products collection efficiency.Such as Zhang Yuming Bacillus licheniformis In the technique of Y8226A strain fermentations production bacitracin, adsorbed by adding D152 resin modes so that bacitracin output increased 27.4% (reference《Bacitracin produces strain improvement and new process for fermenting research》, scientific and technical and engineering;33 phases in 2015, Page 137-141).And in the technique of yeast fermenting and producing 2 phenylethyl alcohol, in situ using addition FD0816 macroreticular resins separates Mode, can reduce inhibitory action of the 2 phenylethyl alcohol to thalline itself, the production concentration of 2 phenylethyl alcohol is promoted into 12.80g/L (held high with reference to closing, Wang Hang, the such as first month of spring transfer techniques in situ are used for yeast and synthesize 2 phenylethyl alcohol [J] process engineering journals, 2009,9 (1):128-132.)。
Compared with traditional fermentation method, the training method that resin original position is separately cultured is solid-liquid two-phase system culture.Hair There is resin particle solid and nutrient solution solid-liquid mixing system in fermentation tank.At present, fermentation tank known in the art and fermentation dress Put and be often directed to the single-phase fermentation of conventional liquid, do not make specific aim adjusted design to tank body, therefore for resin original position point From culture culture when easily occur resin particle and easily block fermentation tank pipeline, resin particle is separated with culture medium expects in the presence of running Phenomenon, resin particle is easily accessible culture medium pipeline, and resin particle is between operating unit the problems such as difficulty of transportation.
Utility model content
The technical problems to be solved in the utility model is to overcome existing common fermentation tank to be separately cultured in resin particle original position When separation is difficult, resin is lost in, easily block valve and there is provided one kind the problem of pipeline, conveying expend a large amount of water or conveying liquid Fermentation tank.
In order to solve the above-mentioned technical problem, the utility model provides following technical scheme:
The utility model provides a kind of fermentation tank being separately cultured for resin original position, including fermentation tank, the tank It is provided with the top of body outside material inlet, the tank body and is provided with motor, the motor is connected with the axis of centres, and passes through axle Envelope is extended to outside tank is provided with agitator on tank inner chamber, the axis of centres, the bottom of the fermentation tank is provided with tank body Bottom, the tank base is provided with the discharging opening of resin particle, while the fermentation tank tank base side wall is provided with a difference Flushing liquor entrance is provided with the top of the leakage fluid dram of height and position, the fermentation tank, the flushing liquor entrance is connected with shower nozzle.
As a kind of optimal technical scheme of the present utility model, the chamber inner port position of the leakage fluid dram is provided with filter Device.
As a kind of optimal technical scheme of the present utility model, the chamber inner port position of the leakage fluid dram is provided with arc Shape kuppe, the entrance of arc kuppe is towards tank skin.
As a kind of optimal technical scheme of the present utility model, the discharging opening of resin particle is through type baiting valve or upper exhibition Formula baiting valve.
As a kind of optimal technical scheme of the present utility model, the pneumatic ash gate that the tank base is provided with, the gas Dynamic ash gate also has the safe fore shaft of locking cylinder formula.
As a kind of optimal technical scheme of the present utility model, the tank base be shaped as inverted conical build or For inverted oblique centrum.
As a kind of optimal technical scheme of the present utility model, the outlet diameter of described pneumatic ash gate is the tank body The 1/4 to 1/2 of waist diameter.
As a kind of optimal technical scheme of the present utility model, the through type baiting valve or the upward-expanding emptying valve its Valve aperture is more than 50cm.
The beneficial effect that the utility model is reached is:The fermentation tank can fast and easy be separately cultured liquid and resin, keep away The problem of exempting from blowing stage or separation phase generation resin particle blocking pipeline or valve.The Zymolysis Equipment can be in original simultaneously Resin is directly reclaimed in position after being separately cultured, so that the pipeline stage avoided expends substantial amounts of conveying liquid, reduces follow-up Step process amount of liquid.
Brief description of the drawings
Accompanying drawing is used for providing further understanding to of the present utility model, and constitutes a part for specification, with this practicality New embodiment is used to explain the utility model together, does not constitute to limitation of the present utility model.In the accompanying drawings:
Fig. 1 is structural representation of the present utility model;
Fig. 2 is oblique formula discharging type fermentation tank structural representation;
Fig. 3 is straight-tapered unloading type fermentation tank structural representation;
Fig. 4 is oblique cone bottom unloading type fermentation tank structural representation;
Fig. 5 is the organigram of discharging tube;
Fig. 6 is the organigram with shield discharging tube;
In figure:0th, ferment tank;1st, motor;2nd, material enters hole;3rd, axle envelope;4th, feed supplement/flushing liquor entrance;5th, shaft coupling Section;6th, the axis of centres;7th, flange;8th, helper;9th, temperature electrode;10th, pH electrodes;11st, dissolved oxygen electrode;12nd, tubulation is cooled down;13rd, stir Mix device;14th, leakage fluid dram;15th, fermentation tank tank base;16th, ventilation duct;17th, air sparger;151st, pneumatic ash gate;152、 The safe fore shaft of locking cylinder formula;162nd, transfer pipeline or receiving tank;141st, leakage fluid dram valve;142nd, filter;143rd, fermentation tank tank Body wall;144th, leakage fluid dram pipeline;145th, the first arc kuppe;146th, the second arc kuppe;147th, arc kuppe entrance; 161st, through type baiting valve;163rd, upward-expanding emptying valve.
Embodiment
Preferred embodiment of the present utility model is illustrated below in conjunction with accompanying drawing, it will be appreciated that described herein excellent Select embodiment to be merely to illustrate and explain the utility model, be not used to limit the utility model.
Embodiment 1
As shown in figures 1 to 6, the utility model provides a kind of fermentation tank being separately cultured for resin original position, including fermentation tank Tank body 0, the top of tank body 0, which is provided with outside material inlet 2, tank body 0, is provided with motor 1, and motor 1 is connected with the axis of centres 6, and Extended to by axle envelope 3 outside tank and agitator 13 is provided with tank inner chamber, the axis of centres 6, the bottom of fermentation tank 0 is provided with tank Body bottom 15, tank base 15 is provided with the discharging opening of resin particle, while the bottom sidewall of tank 0 of fermenting is individual not provided with 2-4 With the leakage fluid dram 14 of height and position, the top of fermentation tank 0 is provided with flushing liquor entrance 4, and flushing liquor entrance 4 is connected with shower nozzle, The fermentation tank can fast and easy be separately cultured liquid and resin, it is to avoid blowing stage or separation phase occur resin particle and block pipe The problem of road or valve.Resin is directly reclaimed after the Zymolysis Equipment can be separately cultured in the original location simultaneously, so that the pipe avoided Road delivery phase expends substantial amounts of conveying liquid, reduces the follow-up step treatment fluid scale of construction.
The chamber inner port position of leakage fluid dram 14 is provided with filter 142, and the filter can be filtered for porous ceramics, stainless steel Net etc..The chamber inner port position of leakage fluid dram 14 is provided with arc kuppe 146, and the entrance 147 of arc kuppe is towards tank Wall, arc kuppe causes the entry position face phase tank skin that liquid enters so that stir culture stage, subsidence stage resin particle Thing is difficult to enter in Drainage tank.The discharging opening of resin particle is through type baiting valve 161 or upward-expanding emptying valve 163, resin Particle is entered in collecting tank by valve, or is directly entered next operating unit by the conveying of pipeline liquid.Tank base 15 The pneumatic ash gate 151 being provided with, pneumatic ash gate 151 also has the safe fore shaft 152 of locking cylinder formula, is putting in tank operation, is treating After fermentation liquid draining is finished, it can directly open pneumatic ash gate and resin is consolidated into material releasing completely, to collecting tank or collecting cart, It is transported to next operating unit, direct filler.Tank base 15 is shaped as inverted conical build or for inverted oblique centrum, Resin particle is reduced to block and be detained.Pneumatic ash gate 151 outlet diameter for fermentation the waist diameter of tank 0 1/4 to 1/2.Through type baiting valve 161 or its valve aperture of upward-expanding emptying valve 163 are more than 50cm.
The device is a kind of fermentation tank being separately cultured for resin original position, when needing to use the tank body, in fermentation knot Shu Hou, closes motor 1, stop agitator 13 stir and ventilation duct 16 air-blowing so that resin particle in culture medium is natural Fall to fermenter base 15.Then the tapping valve 141 farther out apart from fermenter base 15 is first turned on, zymotic fluid is excluded, After band liquid level reduction, leakage fluid dram 14 is progressively opened from high to low so that zymotic fluid liquid level is gradually reduced, while maintaining the heap of resin Product face is basicly stable, will not be resuspended into leakage fluid dram 14.After the completion of discharge opeing, all tapping valves 141 are closed, are opened from fermentation tank The feed supplement at top/flushing liquor entrance 4, is passed through flushing liquor, and by under the resin particle elution on remaining tank skin, agitator is opened afterwards 13 stirrings, are resuspended resin particle.The discharging opening of resin particle is opened afterwards, and resin particle is discharged.
Finally it should be noted that:Preferred embodiment of the present utility model is the foregoing is only, this is not limited to Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art For, it can still modify to the technical scheme described in foregoing embodiments, or to which part technical characteristic Carry out equivalent substitution.It is all it is of the present utility model spirit and principle within, any modification, equivalent substitution and improvements made etc., It should be included within protection domain of the present utility model.

Claims (8)

1. a kind of fermentation tank being separately cultured for resin original position, including fermentation tank (0), it is characterised in that the fermentation It is provided with the top of tank (0) outside material inlet (2), the fermentation tank (0) and is provided with motor (1), it is described electronic Machine (1) is connected with the axis of centres (6), and is extended to and be provided with tank inner chamber, the axis of centres (6) outside tank by axle envelope (3) Agitator (13), the bottom of the fermentation tank (0) is provided with tank base (15), and the tank base (15) is provided with resin The discharging opening of grain, while the leakage fluid dram (14) of fermentation tank (0) bottom sidewall provided with 2-4 different height position, Flushing liquor entrance (4) is provided with the top of the fermentation tank (0), the flushing liquor entrance (4) is connected with shower nozzle.
2. a kind of fermentation tank being separately cultured for resin original position according to claim 1, it is characterised in that the discharge opeing The chamber inner port position of mouth (14) is provided with filter (142).
3. a kind of fermentation tank being separately cultured for resin original position according to claim 1, it is characterised in that the discharge opeing The chamber inner port position of mouthful (14) is provided with the second arc kuppe (146), and arc kuppe entrance (147) is towards tank Wall.
4. a kind of fermentation tank being separately cultured for resin original position according to one of claim 1-3, it is characterised in that tree The discharging opening of fat particle is through type baiting valve (161) or upward-expanding emptying valve (163).
5. a kind of fermentation tank being separately cultured for resin original position according to one of claim 1-3, it is characterised in that institute The pneumatic ash gate (151) that tank base (15) is provided with is stated, the pneumatic ash gate (151) also has locking cylinder formula safety lock Mouth (152).
6. a kind of fermentation tank being separately cultured for resin original position according to claim 5, it is characterised in that the tank body Bottom (15) is shaped as inverted conical build or for inverted oblique centrum.
7. a kind of fermentation tank being separately cultured for resin original position according to claim 5, is further characterized in that, described The outlet diameter of pneumatic ash gate (151) is the 1/4 to 1/2 of described fermentation tank (0) waist diameter.
8. a kind of fermentation tank being separately cultured for resin original position according to claim 4, is further characterized in that, described straight Formula baiting valve (161) and the upward-expanding emptying valve (163) its valve aperture are more than 50cm.
CN201720209393.6U 2017-03-06 2017-03-06 A kind of fermentation tank being separately cultured for resin original position Active CN206529464U (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109796230A (en) * 2019-02-27 2019-05-24 北京农业智能装备技术研究中心 Aerobic fermentation device and its application method
CN110217911A (en) * 2019-06-25 2019-09-10 广元市海鹏生物科技有限公司 A kind of heparin sodium wastewater treatment process of high efficiente callback protein

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109796230A (en) * 2019-02-27 2019-05-24 北京农业智能装备技术研究中心 Aerobic fermentation device and its application method
CN110217911A (en) * 2019-06-25 2019-09-10 广元市海鹏生物科技有限公司 A kind of heparin sodium wastewater treatment process of high efficiente callback protein

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Effective date of registration: 20190918

Address after: Room 58, 1st floor, 111 Fengpu Avenue, Fengxian District, Shanghai 201400

Patentee after: Shanghai Longyin Biotechnology Co., Ltd.

Address before: 201201 Shanghai City, Pudong New Area Ruiqinglu No. 528 building 12 layer 1-3

Patentee before: Burton (Shanghai) Biotechnology Co., Ltd.

TR01 Transfer of patent right