CN206512222U - A kind of single celled micro-fluidic chip of high flux automatic capture - Google Patents
A kind of single celled micro-fluidic chip of high flux automatic capture Download PDFInfo
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- CN206512222U CN206512222U CN201621282696.2U CN201621282696U CN206512222U CN 206512222 U CN206512222 U CN 206512222U CN 201621282696 U CN201621282696 U CN 201621282696U CN 206512222 U CN206512222 U CN 206512222U
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Abstract
The utility model provides a kind of single celled micro-fluidic chip of high flux automatic capture, and the chip is constituted by two layers, and upper strata is stream gateway layer；Lower floor is stream key-course；The stream gateway layer has liquid flow path feeder connection (1) and liquid flow path channel outlet (2)；The stream key-course is made up of unicellular capture flow path channel (3), gas channels (4), drop formation unit (5)；The chip possesses high-throughout drop formation unit, with the controllable gas flow path passage of air pressure, and high-throughout unicellular capture can be achieved, and the automation of unicellular acquisition procedure can be achieved, and reduces cell and reagent dosage, has wide range of applications.
Description
Technical field
The utility model belongs to microflow control technique and polymer chip field, and in particular to a kind of high flux automatic capture list
The micro-fluidic chip of cell.
Background technology
Microfluid based Lab on a chip is sample preparation involved in the fields such as chemistry and biology, reaction, separation, detection
And the basic operation unit such as cell culture, separation, division is integrated on the chip of one piece several square centimeters (even more small), by micro-
Passage formation network, runs through whole system with controlled fluid, the various functions to replace conventional chemical and biology laboratory
A kind of technology platform.The essential characteristic and sharpest edges of micro-fluidic chip are a variety of monotechnicss in overall controllable small platform
Upper flexible combination, scale are integrated.High flux is a kind of integrated form of scale, and the operating unit or one-element group being simply integrated are
Identical.
Biology and medical research in recent years finds there is important meaning for the RESEARCH ON CELL-BIOLOGY of individual cells
Justice.There may also be very big difference between same cell, different cell individuals, and the most significant cell that makes a variation tends to take off
Show important biomolecule phenomenon or point out the generation of major disease, disclose pathogenesis of cancer mechanism, understand that cell differentiation is sent out with tissue
Educate principle, identification gene expression characteristics and cell characteristic.Therefore, accurately capture the single cell population of significant difference and carry out accurate
Identification is the key technology being badly in need of in biomedical research.
In recent years, developing rapidly with microflow control technique, a kind of side for manipulating micro-meter scale fluid is provided for people
Method.The development of micro-processing technology also provides the method for manufacturing the micro-structural similar to cell dimensions simultaneously, and it is at unicellular point
Application in analysis causes increasing concern.Cell factory on chip can be controlled effectively as unicellular microreactor
System diffusion, improves detection sensitivity, has been successfully applied in a variety of single cell analysis.In addition, from substantial amounts of sample (such as blood
Liquid) in accurate capture specific cells and identification identification is carried out to it, be also conventional art (such as flow cytometer) is difficult to.
The utility model is prepared for a kind of high flux and caught automatically using the processing of micro-fluidic and polymer chip, manufacturing technology
Single celled micro-fluidic chip is obtained, whole device is simple in construction, without any complicated and expensive equipment, high flux can be achieved,
Easy to operate automation, unicellular suspension culture, reduction cellular damage can be captured for a long time and is easy to add medicine irritation.
Utility model content
The purpose of this utility model is to provide a kind of single celled micro-fluidic chip of high flux automatic capture, to solve in the past
Complex operation step present in unicellular acquisition procedure is complicated, easily cause the low limitation of cellular damage, capture rate, and it is made
Standby process stabilization, simple to operate, integrated level is high, automation can be achieved.
A kind of single celled micro-fluidic chip of high flux automatic capture, the chip is constituted by two layers, and upper strata goes out for stream
Entry level；Lower floor is stream key-course；
The stream gateway layer has liquid flow path feeder connection and liquid flow path channel outlet；
The stream key-course is by unicellular capture flow path channel, gas channels, (the unicellular capture of drop formation unit
Trap) composition；
The unicellular capture flow path channel is a long-channel being made up of the U-shaped passage of multiple interactive connection, long logical
Road two ends connect liquid flow path feeder connection and liquid flow path channel outlet respectively, and the straight channel of each U-shaped passage has therewith
The drop life of several cylindrical depression pitting structures is dispersed with parallel gas flow path passage, the straight channel of each U-shaped passage
Into unit.
After the chip is vacuumized, gas channels are in negative pressure state, and due to PDMS gas permeability, gas circuit drives surrounding
Fluid path passage formation negative pressure, automatically can suck single cell suspension from fluid path feeder connection, for unicellular feed liquor and capture.
The unicellular capture flow path channel is wide 100~300 μm, a length of 40~50mm of straight channel of U-shaped passage, straight channel
Spacing is 0.5~1mm.
A width of 200~500 μm of the gas channels, a length of 40~50mm, spacing is 0.5~1mm.
The unicellular capture flow path channel and gas channels spacing are 0.5~1.5mm.
The drop formation unit is cylindrical depression pitting structure, has 6000~10000, pitting a diameter of 15~
It is 45 μm, high 80~200 μm, 80~150 μm of pitting spacing.
A kind of application of the single celled micro-fluidic chip of high flux automatic capture, the micro-fluidic chip can be used as high flux
Automatic unicellular capture, can carry out the researchs such as unicellular Culture in situ, administration, propagation, induction differentiation.
The micro-fluidic chip can be used as the automatic unicellular capture of high flux, can carry out unicellular Culture in situ, breed, to
The researchs such as medicine, induction differentiation.
The utility model has the advantage of：
1st, it is easy to operate, quick；
2nd, cell and reagent dosage are few, and experimental cost is cheap；
3rd, Highgrade integration, automation；
4th, have wide range of applications；
Brief description of the drawings
Fig. 1：Chip structure schematic diagram；(a) overall structure figure, (b) is partial enlarged drawing；
Fig. 2：Micro-fluidic chip side partial enlarged drawing.
Wherein：1 is liquid flow path feeder connection, and 2 be liquid flow path channel outlet, and 3 be unicellular capture flow path channel, 4
It is drop formation unit (unicellular capture trap) for gas flow path passage, 5,6 be unicellular；
Fig. 3：Micro-fluidic chip pictorial diagram.
Embodiment
A kind of single celled micro-fluidic chip of high flux automatic capture, as shown in Figure 1, Figure 2, Figure 3 shows；The chip is by two layers
Composition, upper strata is stream gateway layer；Lower floor is stream key-course；
The stream gateway layer has liquid flow path feeder connection 1 and liquid flow path channel outlet 2；
The stream key-course is by unicellular capture flow path channel 3, gas channels 4, (the unicellular capture of drop formation unit
Trap) 5 compositions；
The unicellular capture flow path channel 3 is a long-channel being made up of the U-shaped passage of multiple interactive connection, long logical
Road two ends connect liquid flow path feeder connection 1 and liquid flow path channel outlet 2 respectively, the straight channel of each U-shaped passage have with
Parallel gas flow path passage 4, the drop of several cylindrical depression pitting structures is dispersed with the straight channel of each U-shaped passage
Generation unit 5.
The unicellular capture flow path channel is wide 100~300 μm, a length of 40~50mm of straight channel of U-shaped passage, straight channel
Spacing is 0.5~1mm.
A width of 200~500 μm of the gas channels, a length of 40~50mm, spacing is 0.5~1mm.
The unicellular capture flow path channel and gas channels spacing are 0.5~1.5mm.
The drop formation unit is cylindrical depression pitting structure, has 6000~10000, pitting a diameter of 15~
It is 45 μm, high 80~200 μm, 80~150 μm of pitting spacing.
After chip is vacuumized, gas flow path passage 4 is in negative pressure state, and due to PDMS gas permeability, gas circuit drives week
The formation negative pressure of liquid flow path passage 3 enclosed, MCF-7 single cell suspensions 6 enter unicellular catch under suction function through feeder connection 1
Flow path channel 3 is obtained, after drop formation unit 5, unicellular 6 are retained in the drop of generation, remaining single cell suspension is through logical
Road entrance 2 flows out, and realizes unicellular automatic capture.
A kind of preparation method of the single celled micro-fluidic chip of high flux automatic capture is as follows：
(1) preparation of the single celled micro-fluidic chip SU-8 templates of high flux automatic capture
Micro-fluidic chip prepares the SU-8 templates of channel part projection using photoetching and caustic solution；First, first layer
SU-8 glue thickness is got rid of for 100 μm, mask is placed in SU-8 glue washers, uv-exposure by 95 DEG C of front baking 20min, Temperature fall
30s, dries 20min, Temperature fall after 95 DEG C；Secondly, based on the basis of first layer SU-8 glue, the second layer gets rid of SU-8 glue thickness and is
150 μm, mask is placed in SU-8 glue washers by 95 DEG C of front baking 30min, Temperature fall, uv-exposure 40s, is dried after 95 DEG C
40min, Temperature fall；Finally, using ethyl lactate by above-mentioned SU-8 glue development 10min, 180 DEG C of post bake 2h, Temperature fall is standby
With.
(2) preparation of the single celled PDMS chips of high flux automatic capture
By PDMS and initiator with volume ratio 10：1 is well mixed, and is cast in the SU-8 templates of early stage preparation, 80 DEG C of baking ovens
Solidify 30min, PDMS and SU-8 templates are peeled off, obtained with structured micro-fluidic chip (1)；By PDMS and initiator with body
Product compares 10：1 is well mixed, and is cast in blank glass plate, and PDMS and SU-8 templates are peeled off, obtained by 80 DEG C of curing oven 30min
Blank PDMS chips (2)；Two holes are made a call on chip (2) with card punch, stream gateway are corresponded to respectively, by chip 1 and chip 2
Sealing-in is standby after corona treatment 120s.
Claims (5)
1. a kind of single celled micro-fluidic chip of high flux automatic capture, it is characterised in that：The chip is constituted by two layers, upper strata
For stream gateway layer；Lower floor is stream key-course；
The stream gateway layer has liquid flow path feeder connection (1) and liquid flow path channel outlet (2)；
The stream key-course is made up of unicellular capture flow path channel (3), gas channels (4), drop formation unit (5)；
The unicellular capture flow path channel (3) is a long-channel being made up of the U-shaped passage of multiple interactive connection, long-channel
Two ends connect liquid flow path feeder connection (1) and liquid flow path channel outlet (2) respectively, and the straight channel of each U-shaped passage has
Several cylindrical depression pitting structures are dispersed with gas flow path passage (4) parallel with it, the straight channel of each U-shaped passage
Drop formation unit (5).
2. according to a kind of single celled micro-fluidic chip of high flux automatic capture described in claim 1, it is characterised in that：The list
Cell capture flow path channel is wide 100~300 μm, a length of 40~50mm of straight channel of U-shaped passage, straight channel spacing be 0.5~
1mm。
3. according to a kind of single celled micro-fluidic chip of high flux automatic capture described in claim 1, it is characterised in that：The gas
A width of 200~500 μm of paths, a length of 40~50mm, spacing is 0.5~1mm.
4. according to a kind of single celled micro-fluidic chip of high flux automatic capture described in claim 1, it is characterised in that：The list
Cell capture flow path channel is 0.5~1.5mm with gas channels spacing.
5. according to a kind of single celled micro-fluidic chip of high flux automatic capture described in claim 1, it is characterised in that：The liquid
Drop generation unit is cylindrical depression pitting structure, has 6000~10000, a diameter of 15~45 μm of pitting is high by 80~200
μm, 80~150 μm of pitting spacing.
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| CN201621282696.2U CN206512222U (en) | 2016-11-28 | 2016-11-28 | A kind of single celled micro-fluidic chip of high flux automatic capture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117969A (en) * | 2016-11-28 | 2018-06-05 | 中国科学院大连化学物理研究所 | Single celled micro-fluidic chip of a kind of high throughput automatic capture and preparation method thereof |
| CN108117968A (en) * | 2016-11-28 | 2018-06-05 | 中国科学院大连化学物理研究所 | A kind of single celled method of high-throughput automatic capture based on drop micro-fluidic chip |
-
2016
- 2016-11-28 CN CN201621282696.2U patent/CN206512222U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117969A (en) * | 2016-11-28 | 2018-06-05 | 中国科学院大连化学物理研究所 | Single celled micro-fluidic chip of a kind of high throughput automatic capture and preparation method thereof |
| CN108117968A (en) * | 2016-11-28 | 2018-06-05 | 中国科学院大连化学物理研究所 | A kind of single celled method of high-throughput automatic capture based on drop micro-fluidic chip |
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