CN206486504U - A kind of umbilical cord mesenchymal stem cells are into fat induction and the kit of detection - Google Patents

Abstract

The utility model discloses a kind of umbilical cord mesenchymal stem cells into fat induction and the kit of detection, the inner chamber of the sterilizing box is installed with lateral partitions, the inner chamber bottom of the lower chambers is installed with heating tube, kit is placed with the inner chamber of the upper chamber, the upper end of the sterilizing box is installed with slide, mutually welded with microscopical support on the rear surface of the sterilizing box, the front end of the support is installed with lens barrel, the lower end of the lens barrel is rotatably connected to objective lens, the upper end of told lens barrel is plugged with eyepiece, the utility model voluntarily can carry out sterilization processing in itself to the culture medium and kit in kit, and pass through inducing mesenchymal stem cell, to detect breaking up into fat for mescenchymal stem cell, fully understand into the process of fat differentiation, accomplish to understand mescenchymal stem cell in depth into fat differentiation, it is allowed to more be widely applied in medical treatment and other field.

Description

A kind of umbilical cord mesenchymal stem cells are into fat induction and the kit of detection

Technical field

The utility model is related into fat differentiation agents box technical field, and specially a kind of umbilical cord mesenchymal stem cells divide into fat Change the kit of induction and detection.

Background technology

Life science is the science the most rapid of 20 end of the centurys and beginning of this century Development of Natural Science, wherein to stem cell Research and application be one of topmost research field, stem cell is the cell that a class has self-renewing and differentiation potential, Polytype specialized cell can be induced to differentiate into, so as to realize the reparation and regeneration of tissue, mescenchymal stem cell is a class Stem cell with self-renewing, propagation and multi-lineage potential, can be used in medical treatment, but people are dry to mesenchyma now The induction situation broken up into fat of cell is not to be well understood by, it is impossible to which mescenchymal stem cell is applied into fat differentiation is more preferable In medical treatment, therefore, we have proposed a kind of umbilical cord mesenchymal stem cells into fat induction and the kit of detection.

Utility model content

The purpose of this utility model is to provide a kind of umbilical cord mesenchymal stem cells into fat induction and the reagent of detection Box, to solve the problems mentioned in the above background technology.

To achieve the above object, the utility model provides following technical scheme：A kind of umbilical cord mesenchymal stem cells divide into fat Change the kit of induction and detection, including bacterium box, the right hand edge of the upper end left hand edge and upper lid of the sterilizing box is hinged, institute State and lateral partitions be installed with the lumen centers of sterilizing box, and lateral partitions the inner chamber of sterilizing box be divided into lower chambers and Upper chamber, the inner chamber bottom of the lower chambers is installed with heating tube, and heating tube front end through sterilizing box outer wall with Wire is electrically connected, and kit is placed with the inner chamber of the upper chamber, and kit is located at the upper surface center of lateral partitions, The inner chamber uniform vertical of the kit is installed with two pieces of dividing plates, and kit inner chamber since the upper left corner quilt clockwise It is divided into first chamber, second chamber, the 3rd chamber and the 4th chamber, the upper end left upper of the sterilizing box is installed with Slide, the rear surface of the sterilizing box is mutually welded with the outer surface of microscopical support, and the microscope includes support, mirror Cylinder, objective lens and eyepiece, the front end of the support are installed with lens barrel, and the lower end of the lens barrel is rotatably connected to objective lens, The upper end of told lens barrel is plugged with eyepiece, and eyepiece is located at the top of slide.

It is preferred that, the scope of the support height up and down is within 5 centimetres, and the scope that support level is rotated is 45 Spend between -45 degree.

It is preferred that, it is closely fitted with sealing rubber strip at the top surface edge of the upper lid.

Compared with prior art, the beneficial effects of the utility model are：The utility model devises a kind of umbilical cord mesenchyma Stem cell voluntarily can kill in itself into fat induction and the kit of detection to the culture medium and kit in kit Bacterium is handled, and by inducing mesenchymal stem cell, to detect breaking up into fat for mescenchymal stem cell, fully understands into fat differentiation Process, accomplishes to understand mescenchymal stem cell in depth into fat differentiation, is allowed to more be widely applied to medical treatment and other field In.

Brief description of the drawings

Fig. 1 is the utility model structural representation；

Fig. 2 is kit schematic diagram in the utility model.

In figure：1 sterilizing box, lid, 3 lateral partitions, 4 lower chambers, 5 upper chamber, 6 heating tubes, 7 wires, 8 kits, 81 on 2 Dividing plate, 82 first chambers, 83 second chambers, 84 the 3rd chambers, 85 the 4th chambers, 9 slides, 10 microscopes, 101 supports, 102 Lens barrel, 103 objective lens, 104 eyepieces.

Embodiment

Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belongs to the scope of the utility model protection.

Fig. 1 and 2 is referred to, the utility model provides a kind of technical scheme：A kind of umbilical cord mesenchymal stem cells break up into fat Induction and the kit of detection, including bacterium box 1, the right hand edge of the upper end left hand edge of the sterilizing box 1 and upper lid 2 are hinged, Upper lid 2 can closely be fastened with the upper end of sterilizing box 1, make the high-temperature steam bactericidal effect in sterilizing box 1 more preferable, the sterilization Lateral partitions 3 are installed with the lumen centers of box 1, and the inner chamber of sterilizing box 1 is divided into the He of lower chambers 4 by lateral partitions 3 Upper chamber 5, lateral partitions 3 facilitate vapor to rise through lateral partitions 3 to sterilize kit 8, the lower chambers 4 it is interior Bottom of chamber end is installed with heating tube 6, and the front end of heating tube 6 is electrically connected through the outer wall of sterilizing box 1 with wire 7, and wire 7 leads to After electricity, heating tube 6 is heated, the water in the heating lower chambers 5 of heating tube 6 quickly produces vapor, the upper chamber 5 Kit 8 is placed with inner chamber, and kit 8 is located at the upper surface center of lateral partitions 3, it is dry thin that kit is used for mesenchyma Fiber differentiation and detection that born of the same parents break up into fat, the inner chamber uniform vertical of the kit 8 are installed with two pieces of dividing plates 81, and examination The inner chamber of agent box 8 is separated into first chamber 82, second chamber 83, the 3rd chamber 84 and the 4th chamber clockwise since the upper left corner The DMEM basic culture solutions containing 10 FBS are put into room 85, first chamber 82 and second chamber 83, P3 generations are placed into Mescenchymal stem cell, when it grows to account for its chamber floor space 80 percent, basic culture solution is abandoned in suction, with sterile PBS first chamber 82 and second chamber 83 3 times, add adipogenic induction nutrient solution, change an adipogenic induction culture within every three days Liquid, the 3rd chamber 84 and the 4th chamber 85 group as a comparison, are put into the DMEM basic culture solutions containing 10 FBS, then The mescenchymal stem cell in P3 generations is put into, a basic culture solution is changed within every three days, is inhaled after fortnight abandon first chamber 82 and second altogether The basic culture solution in adipogenic induction nutrient solution and the 3rd chamber 84 and the 4th chamber 85 in chamber 83, is all rinsed with PBS, then 1 hour is fixed with 4 percent paraformaldehyde room temperature, then is cleaned with 70 percent isopropanol, then with chilli oil O room temperatures Dyeing 10 minutes, finally washes away excess dyestuff, the mescenchymal stem cell after four chamber dyeing with 70 percent isopropanol The sample turned out is individually placed on slide 9, is detected by the object lens 103 and eyepiece 104 of microscope 10, Ke Yitong The eyepiece 104 for exchanging different size is crossed, the more preferable detection to sample on slide 9, the upper end upper left corner of the sterilizing box 1 is realized Place is installed with slide 9, and the rear surface of the sterilizing box 1 and the outer surface of the support 101 of microscope 10 are mutually welded, described Microscope 10 includes support 101, lens barrel 102, objective lens 103 and eyepiece 104, and the front end of the support 101 is installed with mirror Cylinder 102, the lower end of the lens barrel 102 is rotatably connected to objective lens 103, tells that the upper end of lens barrel 102 is plugged with eyepiece 104, and Eyepiece 104 is located at the top of slide 9.

Specifically, the scope of the height up and down of support 101 is within 5 centimetres, and support 101 horizontally rotates Scope 45 degree to -45 degree between, by up and down and left and right adjusting microscope 10 support 101, object lens 103 are directed at slide Sample on 9.

Specifically, sealing rubber strip is closely fitted with the top surface edge of the upper lid 2, sealing rubber strip is high temperature Steam locks improve the efficiency of sterilization in sterilizing box 1.

Operation principle：Lid 2 in opening, appropriate water is poured into sterilizing box 1, makes the lower surface one apart from lateral partitions 3 Set a distance, places kit 8 in lateral partitions 3, and same volume is added in four chambers into kit 8 contains percentage Ten FBS DMEM basic culture solutions, cover lid 2, be powered to wire 7, heating tube 6 is generated heat, by under 6 pairs of heating tube Water in chamber 4 is heated, and substantial amounts of high-temperature vapor is produced rapidly, using high-temperature vapor to kit 8 and therein Basic culture solution carries out sterilization processing, and upper lid 2 is opened after sterilization processing, umbilical cord mesenchymal stem cells into fat induction and inspection The kit of survey is placed on natural cooling under isoperibol, after the cooling period, is put into and contains into first chamber 82 and second chamber 83 There are 10 FBS DMEM basic culture solutions, place into the mescenchymal stem cell in P3 generations, grown at it and account for its chamber bottom Area 80 percent when, basic culture solution is abandoned in suction, with sterile PBS first chamber 82 and second chamber 83 3 times, plus Enter adipogenic induction nutrient solution, change an adipogenic induction nutrient solution within every three days, with the 3rd chamber 84 and the 4th chamber 85 as a comparison Group, is put into the DMEM basic culture solutions containing 10 FBS thereto, places into the mescenchymal stem cell in P3 generations, every three It changes a basic culture solution, inhales the adipogenic induction nutrient solution abandoned in first chamber 82 and second chamber 83 and the after fortnight altogether Basic culture solution in three chambers 84 and the 4th chamber 85, is all rinsed with PBS, then is fixed with 4 percent paraformaldehyde room temperature 1 hour, then cleaned with 70 percent isopropanol, then dyed 10 minutes with chilli oil O room temperatures, finally with 70 percent Isopropanol washes away excess dyestuff, and the sample that the mescenchymal stem cell after four chamber dyeing is turned out is individually placed slide 9 On, detected, can be realized by exchanging the eyepiece 104 of different size by the object lens 103 and eyepiece 104 of microscope 10 More preferable detection to sample on slide 9, the utility model makes people fully understand into the process of fat differentiation, is filled between accomplishing pair Being understood in depth into fat differentiation for matter stem cell, makes it widely apply in medical treatment and other field.

It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and And in the case of without departing substantially from spirit or essential attributes of the present utility model, can realize that this practicality is new in other specific forms Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation Involved claim.

Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It may be appreciated other embodiment.

Claims (3)

1. a kind of umbilical cord mesenchymal stem cells exist into fat induction and the kit of detection, including sterilizing box (1), its feature In：The upper end left hand edge of the sterilizing box (1) and the right hand edge of upper lid (2) are hinged, at the lumen centers of the sterilizing box (1) Lateral partitions (3) are installed with, and the inner chamber of sterilizing box (1) is divided into lower chambers (4) and upper chamber by lateral partitions (3) (5), the inner chamber bottom of the lower chambers (4) is installed with heating tube (6), and the front end of heating tube (6) passes through sterilizing box (1) Outer wall electrically connected with wire (7), be placed with kit (8) in the inner chamber of the upper chamber (5), and kit (8) is located at horizontal stroke To the upper surface center of dividing plate (3), the inner chamber uniform vertical of the kit (8) is installed with two pieces of dividing plates (81), and The inner chamber of kit (8) is separated into first chamber (82), second chamber (83), the 3rd chamber clockwise since the upper left corner (84) and the 4th chamber (85), the upper end left upper of the sterilizing box (1) is installed with slide (9), the sterilizing box (1) rear surface and the outer surface of the support (101) of microscope (10) are mutually welded, the microscope (10) include support (101), Lens barrel (102), objective lens (103) and eyepiece (104), the front end of the support (101) are installed with lens barrel (102), described The lower end of lens barrel (102) is rotatably connected to objective lens (103), tells that the upper end of lens barrel (102) is plugged with eyepiece (104), and mesh Mirror (104) is located at the top of slide (9).

2. a kind of umbilical cord mesenchymal stem cells according to claim 1 are into fat induction and the kit of detection, it is special Levy and be：The scope of support (101) height up and down is within 5 centimetres, and the scope that support (101) horizontally rotates exists 45 degree between -45 degree.

3. a kind of umbilical cord mesenchymal stem cells according to claim 1 are into fat induction and the kit of detection, it is special Levy and be：Sealing rubber strip is closely fitted with the top surface edge of the upper lid (2).