CN206459960U - A kind of droplet type digital pcr chip analyzer - Google Patents
A kind of droplet type digital pcr chip analyzer Download PDFInfo
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- CN206459960U CN206459960U CN201720082854.8U CN201720082854U CN206459960U CN 206459960 U CN206459960 U CN 206459960U CN 201720082854 U CN201720082854 U CN 201720082854U CN 206459960 U CN206459960 U CN 206459960U
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- dichroic mirror
- light splitting
- light
- optical assembly
- digital pcr
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Abstract
The utility model discloses a kind of droplet type digital pcr chip analyzer, including slide holder, light splitting dichroic mirror head, excite optical assembly and receive optical assembly, the light splitting dichroic mirror head has the incident optical port for receiving and exciting optical assembly to send laser, exciting light is projected to objective table and receives the two-way light exit that objective table sends fluorescence, and the outgoing optical port of fluorescence is projected to reception optical assembly, the quantity of the light splitting dichroic mirror head is at least two, and on a rotatable platform, along the rotation direction arranged of rotatable platform, different Excitation Filter with High is provided with the incident optical port of different light splitting Look mirror heads.The utility model droplet type digital pcr chip analyzer sets at least two light splitting dichroic mirror heads, Excitation Filter with High can be replaced by rotary motion platform, so as to be advantageously used in detecting the droplet type digital pcr biochip of different fluorescence labelings.
Description
Technical field
The utility model is related to biological chip testing technology field, more particularly to a kind of droplet type digital pcr chip point
Analyzer.
Background technology
Digital pcr (Digital PCR, dPCR) is a kind of nucleic acid quantification counted based on single-molecule PCR method
Method, is a kind of method of absolute quantitation.The main micro-fluidic or droplet side using the popular research field of present analysis chemistry
Method, the nucleic acid solution after Macrodilution is dispersed in the microreactor of chip or droplet, the nucleic acid-templated number of each reactor
It is less than or equal to 1.So by after PCR cycle, the reactor for having nucleic acid templates will provide fluorescence signal, not have
There is the reactor of template just without fluorescence signal.According to relative scale and the volume of reactor, it is possible to extrapolate original solution
Nucleic acid concentration.It is different from traditional quantitative PCR, method of the digital pcr by directly counting, it is possible to achieve starting DNA profiling
Absolute quantitation.
By advantage of the microflow control technique in terms of microfluid manipulation, multiple research groups and company are had in recent international
Develop the digital pcr system based on microflow control technique.Compare be typically include Fluidigm companies release based on microchamber type
BioMarkTM systems and Bio-Rad companies release the QX100TM systems based on drop type.
Microlayer model technology refers to utilize immiscible phase formation Water-In-Oil or oil-in-water metastable independent microbody hydrops
Drop.Emulsifying manner has a variety of, wherein the drop formation technology based on micro-fluidic chip is rapidly developed recent years, extensively should
It is a kind of very important technology for living nature and material circle.Its principle mainly passes through certain angle between two-phase liquid stream
Under house type squeezing action, wherein a phase continuous flow breaks to form drop.Conventional drop preparation method has orthohormbic structure at present
(T-junction) focused on (Flow-focusing) etc. with streaming.The systematic comparison of these method integral devices is complicated, to fluid
The requirement of control system is higher.
At present, the detection for biochip mainly has two ways, and a kind of mode is detected by fluorescent method,
This detection mode needs fluorescence molecule that biochip is marked；Another detection mode is by biochip scanner
Biochip is detected.
Publication No. CN103115901A Chinese invention patent discloses a kind of based on the biological core of resonant light scattering detection
The device of piece, including light source drive, optical fiber, excitation source, coupled lens group, biochip objective table, photoelectric sensor, on
Position machine, motion platform and multi-axle motor driving；Light source drive control excitation source produces exciting light, and exciting light is through optical fiber output
To coupled lens group and beam shaping is carried out to it and is incident on biochip objective table, critical angle is more than by giving birth to incidence angle
Thing sides of chip is incident, and after being excited by exciting light resonant light scattering occurs for sample, is dissipated to the resonance of external radiation specific wavelength
Light is penetrated, resonance light scattering is incident in photoelectric sensor, host computer receives the electric signal after conversion and passes through upper computer software pair
It is acquired analysis, obtains scan image, upper computer software control multi-axle motor driving operation and the movement of powered motion platform,
The movement of biochip objective table is driven, the whole spotted area of biochip is scanned.
The species of current biochip is various, and effective coverage size differs, and existing biochip imaging device can not basis
The convenient regulation excitation area in effective coverage and areas imaging.Once the biochip size to be detected changes, it is necessary to
Replacing suitably excites camera lens and imaging sensor, so that the excitation area of excitation source adapts to biochip to be detected, with
Reach and both cover all scopes to be detected of biochip, while it is most sharp keen that excitation source can again reached, while in figure
As obtaining most clearly fluoroscopic image on sensor.
Utility model content
The utility model can be used in detecting a variety of different wave length fluorescence there is provided a kind of in view of the shortcomings of the prior art
Droplet type digital pcr chip analyzer.
A kind of droplet type digital pcr chip analyzer, including slide holder, light splitting dichroic mirror head, excite optical assembly and reception
Optical assembly, the light splitting dichroic mirror head, which has, to be received the incident optical port for exciting optical assembly to send laser, projects and swash to objective table
Luminous and reception objective table sends the two-way light exit of fluorescence and the outgoing optical port of fluorescence, institute is projected to reception optical assembly
The quantity for stating light splitting dichroic mirror head is at least two, and on a rotatable platform, along the rotation direction of rotatable platform
Different Excitation Filter with High is provided with arranged, the incident optical port of different light splitting Look mirror heads.
Light splitting dichroic mirror head is internally provided with light splitting dichroic mirror (dichroic mirror), and light splitting dichroic mirror is a kind of dry
The combination of filter is related to, when light splitting dichroic mirror tilts 45° angle relative to incident beam and transmitting light beam, its reflecting short length
Excitation wavelength, enters sample with 90 ° of angles, while entering detecting system through the fluorescence of long wavelength.
During detection, the installation's power source is opened, chip to be detected is positioned on slide holder, adjustment slide holder position makes core
Piece is in correct position, and the light splitting dichroic mirror head that rotating rotatable platform selection needs is glimmering according to used in reaction solution on chip
Light color selects suitable Excitation Filter with High, then, excites the laser that optical assembly is sent to pass through swashing on light splitting dichroic mirror head
Light optical filter, the light of unnecessary wavelength is filtered out, and leaving behind needs the laser of wavelength, and laser is got on chip, glimmering in reaction solution
Light group absorptions exciting light, discharges transmitting light, and transmitting light reaches reception optical assembly by light splitting dichroic mirror head and is imaged.
The slide holder is located at the underface of light splitting dichroic mirror head, and the reception optical assembly is being located at light splitting dichroic mirror head just
Top, the side for exciting optical assembly to be located at light splitting dichroic mirror head.
The periphery of the rotatable platform has on the annular loading plate of support light splitting dichroic mirror head, the annular loading plate
It is provided with through hole corresponding with two-way light exit, above the light splitting dichroic mirror head and its pressure is matched for clamping tightly with annular loading plate
Plate.
The reception optical assembly includes object lens, fluorescent optical filter and imaging unit, and the fluorescent optical filter has
It is multiple, it is fixed on a rotating disk, and along the circumferencial direction arranged of rotating disk, replaced by rotating rotating disk in reception optical assembly
Fluorescent optical filter.
The slide holder includes loading plate and apparatus for adjusting position, and the apparatus for adjusting position includes base, the first cunning
Seat and second slide, are provided with first level sliding equipment between the first slide and base, second slide and first slide it
Between be provided with the second horizontal sliding equipment, glide direction between first level sliding equipment and the second horizontal sliding equipment is vertical,
Loading plate is in first slide.
The loading plate is provided with stopper slot.Stopper slot is used to fix chip.
The utility model droplet type digital pcr chip analyzer is pacified by setting at least two light splitting dichroic mirror heads
On a rotatable platform, along the rotation direction arranged of rotatable platform, at the incident optical port of different light splitting Look mirror head
Provided with different Excitation Filter with High so that the laser corresponding to the wavelength of needs can be transferred to by rotary motion platform as needed
Optical filter, so that the utility model droplet type digital pcr chip analyzer can be advantageously used in detecting different fluorescence marks
The droplet type digital pcr biochip of note.
Brief description of the drawings
Fig. 1 is the utility model cross-sectional view；
Fig. 2 is A partial enlarged drawings in Fig. 1；
Fig. 3 is the structural representation of the utility model light splitting dichroic mirror head and rotatable platform.
Embodiment
As shown in Figures 1 to 3, a kind of droplet type digital pcr chip analyzer, including slide holder 1, light splitting dichroic mirror first 2, swash
Luminescence component 3 and reception optical assembly 4.Slide holder 1 is located at the underface of light splitting dichroic mirror first 2, receives optical assembly 4 and is located at light splitting two
The surface of Look mirror first 2, excites optical assembly 3 to be located at the side of light splitting dichroic mirror first 2.
Slide holder 1 includes loading plate 11 and apparatus for adjusting position, and the apparatus for adjusting position is slided including base 12, first
Seat 13 and second slide 14, is provided with first level sliding equipment 15 between first slide 13 and base 12, second slide 14 and the
It is provided between one slide 13 between the second horizontal sliding equipment 16, the horizontal sliding equipment 16 of first level sliding equipment 15 and second
Glide direction it is vertical, loading plate 11 is in first slide 13.Loading plate 11 is provided with stopper slot 17.
Light splitting dichroic mirror first 2, which has to receive, to be excited optical assembly 3 to send the incident optical port 21 of laser, is projected to objective table 1
Excite and receive the outgoing optical port that objective table 1 sends the two-way light exit 22 of fluorescence and fluorescence is projected to reception optical assembly 4
23.The quantity of light splitting dichroic mirror first 2 is at least two, and on a rotatable platform 25, along turning for rotatable platform 25
Different Excitation Filter with High 24 is provided with dynamic direction arranged, the incident optical port 21 of different light splitting Look mirror first 2.The color of light splitting two
The inside of camera lens 2 has light splitting dichroic mirror 29, the placed angle of light splitting dichroic mirror 29 and incident light, bi-directional light (exciting light and transmitting light)
With emergent light angle at 45 °.
The periphery of rotatable platform 25 has on the annular loading plate 26 of support light splitting dichroic mirror first 2, annular loading plate 26
With through hole 28 corresponding with two-way light exit 22, first 2 top of light splitting dichroic mirror is provided with is matched for clamping tightly it with annular loading plate 26
Pressing plate 27.
Receiving optical assembly 4 includes object lens 41, fluorescent optical filter 42 and imaging unit 44, and fluorescent optical filter 42 has
It is multiple, it is fixed on a rotating disk 43, and along the circumferencial direction arranged of rotating disk 43, reception light is replaced by rotating rotating disk 43
Fluorescent optical filter 42 in component 4.
During detection, it is first turned on excitation source and is preheated, open the power supply of the device, chip to be detected is positioned over
On the loading plate 11 of slide holder 1, up-regulation is put in level by the horizontal sliding equipment 16 of first level sliding equipment 15 and second
The position of whole loading plate 11, makes chip be in correct position, and then the position of in the vertical direction adjustment object lens 41 makes to turn
Light splitting dichroic mirror first 2 that the dynamic selection of rotatable platform 25 needs, i.e., fluorescence color is selected according to used in reaction solution on chip
Then suitable Excitation Filter with High 24, the fluorescent optical filter 42 that the selection of rotary turnplate 43 needs, excites what optical assembly 3 sent to swash
Light is filtered out the light of unnecessary wavelength by the Excitation Filter with High 24 on light splitting dichroic mirror first 2, and leaving behind needs the laser of wavelength
As exciting light, the exciting light of short wavelength, which is got on light splitting dichroic mirror 29, to be reflected, the sample entered with 90 ° of angles in chip, is swashed
The luminophore that fluoresces sends transmitting light (fluorescence), and the fluorescence of long wavelength is transmitted into reception optical assembly 4 from light splitting dichroic mirror 29,
Filtered out by fluorescent optical filter 42 after unwanted wave band, reach imaging unit 44 and be imaged.
Claims (6)
1. a kind of droplet type digital pcr chip analyzer, including slide holder, light splitting dichroic mirror head, excite optical assembly and receive light
There is component, the light splitting dichroic mirror head reception to excite optical assembly to send the incident optical port of laser, be excited to objective table projection
Light and reception objective table send the two-way light exit of fluorescence and to the outgoing optical port for receiving optical assembly injection fluorescence, and it is special
Levy and be, the quantity of the light splitting dichroic mirror head is at least two, and on a rotatable platform, along rotatable platform
Different Excitation Filter with High is provided with rotation direction arranged, the incident optical port of different light splitting Look mirror heads.
2. droplet type digital pcr chip analyzer as claimed in claim 1, it is characterised in that the slide holder is located at light splitting
The underface of dichroic mirror head, the reception optical assembly be located at light splitting dichroic mirror head surface, it is described excite optical assembly located at point
The side of light dichroic mirror head.
3. droplet type digital pcr chip analyzer as claimed in claim 1 or 2, it is characterised in that the circle of the rotatable platform
Side face, which has on the annular loading plate of support light splitting dichroic mirror head, the annular loading plate, has lead to corresponding with two-way light exit
It is provided with above hole, the light splitting dichroic mirror head and its pressing plate is matched for clamping tightly with annular loading plate.
4. droplet type digital pcr chip analyzer as claimed in claim 1, it is characterised in that the reception optical assembly includes
Object lens, fluorescent optical filter and imaging unit, the fluorescent optical filter has multiple, is fixed on a rotating disk, and along
The circumferencial direction arranged of rotating disk, the fluorescent optical filter received in optical assembly is replaced by rotating rotating disk.
5. droplet type digital pcr chip analyzer as claimed in claim 1, it is characterised in that the slide holder includes carrying
Plate and apparatus for adjusting position, the apparatus for adjusting position include base, first slide and second slide, the first slide with
First level sliding equipment is provided between base, the second horizontal sliding equipment, first are provided between second slide and first slide
Glide direction between horizontal sliding equipment and the second horizontal sliding equipment is vertical, and loading plate is in first slide.
6. droplet type digital pcr chip analyzer as claimed in claim 5, it is characterised in that the loading plate is provided with limit
Position groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720082854.8U CN206459960U (en) | 2017-01-20 | 2017-01-20 | A kind of droplet type digital pcr chip analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720082854.8U CN206459960U (en) | 2017-01-20 | 2017-01-20 | A kind of droplet type digital pcr chip analyzer |
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| CN206459960U true CN206459960U (en) | 2017-09-01 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904156A (en) * | 2017-10-31 | 2018-04-13 | 华东医药（杭州）基因科技有限公司 | A kind of one full-automation digital pcr detecting system and implementation |
| CN110734854A (en) * | 2019-09-25 | 2020-01-31 | 中国科学院苏州生物医学工程技术研究所 | -body rapid detection system for real-time fluorescence quantitative analysis of ultrahigh-flux single-cell nucleic acid molecules |
| CN110951580A (en) * | 2019-09-29 | 2020-04-03 | 中国科学院苏州生物医学工程技术研究所 | High-throughput single-cell transcriptome and gene mutation integration analysis integrated device |
| CN111215161A (en) * | 2020-01-15 | 2020-06-02 | 北京中科生仪科技有限公司 | Optical detection system for nucleic acid amplification instrument |
| CN113092424A (en) * | 2021-03-18 | 2021-07-09 | 上海医本医疗器械有限公司 | Novel high-flux biochip analyzer |
-
2017
- 2017-01-20 CN CN201720082854.8U patent/CN206459960U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904156A (en) * | 2017-10-31 | 2018-04-13 | 华东医药（杭州）基因科技有限公司 | A kind of one full-automation digital pcr detecting system and implementation |
| CN107904156B (en) * | 2017-10-31 | 2021-06-29 | 领航基因科技（杭州）有限公司 | Integrated full-automatic digital PCR detection system and implementation method |
| CN110734854A (en) * | 2019-09-25 | 2020-01-31 | 中国科学院苏州生物医学工程技术研究所 | -body rapid detection system for real-time fluorescence quantitative analysis of ultrahigh-flux single-cell nucleic acid molecules |
| CN110951580A (en) * | 2019-09-29 | 2020-04-03 | 中国科学院苏州生物医学工程技术研究所 | High-throughput single-cell transcriptome and gene mutation integration analysis integrated device |
| CN110951580B (en) * | 2019-09-29 | 2022-05-20 | 中国科学院苏州生物医学工程技术研究所 | High-throughput single-cell transcriptome and gene mutation integration analysis integrated device |
| CN111215161A (en) * | 2020-01-15 | 2020-06-02 | 北京中科生仪科技有限公司 | Optical detection system for nucleic acid amplification instrument |
| CN113092424A (en) * | 2021-03-18 | 2021-07-09 | 上海医本医疗器械有限公司 | Novel high-flux biochip analyzer |
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| CP01 | Change in the name or title of a patent holder |
Address after: 310000 room 1025, No. 888, Feng Qi East Road, Jianggan District, Hangzhou, Zhejiang Patentee after: Navigation gene technology (Hangzhou) Co., Ltd. Address before: 310000 room 1025, No. 888, Feng Qi East Road, Jianggan District, Hangzhou, Zhejiang Patentee before: Huadong medicine (Hangzhou) gene science and Technology Co Ltd |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211221 Address after: 518000 g1316, Lianxing building, building B, Yihua new village, district 46, Haifu community, Xin'an street, Bao'an District, Shenzhen, Guangdong Province Patentee after: Pilot medical technology (Shenzhen) Co.,Ltd. Address before: 310000 room 1025, No. 888, Fengqi East Road, Jianggan District, Hangzhou City, Zhejiang Province Patentee before: PILOT GENE TECHNOLOGIES (HANGZHOU) Co.,Ltd. |