CN206440976U - One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment - Google Patents
One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment Download PDFInfo
- Publication number
- CN206440976U CN206440976U CN201720076287.5U CN201720076287U CN206440976U CN 206440976 U CN206440976 U CN 206440976U CN 201720076287 U CN201720076287 U CN 201720076287U CN 206440976 U CN206440976 U CN 206440976U
- Authority
- CN
- China
- Prior art keywords
- module
- epiphragma
- nitrogen
- sample
- metabolic disorders
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model provides a kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment, belong to Inherited Metabolic Disorders testing equipment field, it includes central processor CPU, built-in storage, read only memory ROM, external storage device, mainboard, temperature detection and management module, reagent add module, sample-manipulating device, centrifugation control module, nitrogen supply (NS) module and epiphragma module；By equipment automatic running and standardize progress epiphragma operation, addition reagent, centrifugation, nitrogen and dry up etc., so as to replace artificial operation, greatly reduce the working strength of testing staff, reduction IEM detection error rates.The processing equipment that the utility model is provided has the advantages that automation, standardization and reduction person works' intensity.
Description
Technical field
The utility model belongs to automation Inherited Metabolic Disorders testing equipment field, more particularly to a kind of automation heredity metabolism
Disease detection sample pretreatment equipment.
Background technology
Inherited metabolic disease, also known as Organic acidemia（English abbreviation：IEM）, it is because maintaining body normal
The encoding gene of certain enzyme, transporter, film or acceptor etc. is undergone mutation necessary to metabolism, the product function hair for encoding it
It is raw to change, and there is abnormal and clinical symptoms the class disease of corresponding laboratory examination.It is related to amino acid, organic acid, fat
The exception of many kinds of substance such as fat acid, urea cycle, carbohydrate, lysosome, steroids metabolism.Such disease is mostly single base
Because of hereditary disease, minority is chondriogen hereditary disease.
The change and the attention to inborn defect composed with human diseases, inborn error of metabolism disease is increasingly by masses
Concern.Because many inborn error of metabolism disease has no specificity in terms of clinical manifestation, common Symptoms are not clear
The Recurrent Vomiting of reason, tic, Tendon defection, intelligence development fall behind etc., have in terms of laboratory examination metabolic acidosis,
Hypoglycemia, hyperlactacidemia, hyperammonemia etc..Specificity due to a lack of inherited metabolic disease is checked, cannot get such disease
Clarify a diagnosis and give treatment in time, finally bring white elephant to infant and its family, have impact on all one's life of infant.
IEM screening method is from the Bacteria suppression method of beginning, ELISA, the chromatogram of radioimmunology finally
Method, mass spectrography and the methods of genotyping occurred recently.The rapid advances of science and technology, have constantly promoted neonate's sieve energetically
Look into popularization of the means in various countries, area.Wherein, mass spectrography is by the way that sample to be converted into the gas ion of motion and by mass-to-charge ratio
Size is separated and records its information, so as to carry out a kind of analysis method of qualitative and quantitative analysis to sample, it detects sample
The operation of product is various, including sample preparation is preserved, reagent is added, shake heating, epiphragma etc..Simultaneously because IEM examination is more next
More popularize, the sample of detection is more and more, excessive detection sample easily makes testing staff's fatigue, so as to cause to detect mistake.
If the work of the partial repeatability mechanizations such as the epiphragma of sample pretreatment, reagent addition is switched to replace artificial progress by equipment
Operation would be possible to reduction detection error rate, while realizing the operation of standardization.
The content of the invention
The problem of having above-mentioned based on prior art, the utility model provides a kind of automation Inherited Metabolic Disorders detection sample
Pre-processing device, it includes central processor CPU, built-in storage, read only memory ROM, external storage device, mainboard, temperature
Detection and management module, reagent add module, sample-manipulating device, centrifugation control module, nitrogen supply (NS) module and epiphragma module；
By equipment automatic running and standardize progress epiphragma operation, addition reagent, centrifugation, nitrogen and dry up etc., so as to replace artificial behaviour
Make, greatly reduce the working strength of testing staff, reduction IEM detection error rates.The processing equipment tool that the utility model is provided
There are automation, standardization and reduction person works' intensity.
One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment, it include central processor CPU, built-in storage,
Read only memory ROM, external storage device, mainboard, temperature detection and management module, reagent add module, sample-manipulating device,
Centrifuge control module, nitrogen supply (NS) module and epiphragma module；
Wherein central processor CPU, built-in storage, read only memory ROM, temperature detection and management module, reagent addition
Module, centrifugation control module, nitrogen supply (NS) module are connected and communicated by the circuit of mainboard with epiphragma module；
External storage device and sample-manipulating device are connected by electrical wiring to mainboard, are communicated with central processor CPU, in
Central processor CPU control sample-manipulating devices operate and clamp sample to difference in functionality area, and central processor CPU is according to specimen holder
The feedback of the information of holding equipment controls reagent add module, centrifugation control module 9, nitrogen supply (NS) module and epiphragma module, examination successively
Agent add module, centrifugation control module 9, nitrogen supply (NS) module and the operation of epiphragma module；Central processor CPU is by system operation number
According to recorded in external storage device.
Wherein, nitrogen supply (NS) module includes nitrogen supply (NS) switch module and nitrogen blows rate control module, nitrogen supply (NS)
Light modules and nitrogen blow rate control module and connected by wire circuit.
Wherein, epiphragma module includes Teflon film epiphragma module, aluminium film capping module and position computation module, Teflon film
Epiphragma module, aluminium film capping module and the integrated epiphragma module of position computation module, position computation module are connected to displacement transducer,
Displacement data is simultaneously transferred to position computation module by the displacement of displacement transducer detection film.
Wherein, nitrogen supply (NS) module is connected to nitrogen gas generator or nitrogen pot by wire circuit.
Wherein, described external storage device includes the one or more of hard disk, USB flash disk and immediate access device.
The utility model has the advantage that：The utility model controls each equipment to run by central processor CPU,
The operations such as reagent addition, epiphragma, centrifugation, nitrogen drying are completed, reduce the working strength of testing staff, it is to avoid because of personnel
Work fatigue and the human error caused.Artificial progress reagent addition, epiphragma, centrifugation, nitrogen is replaced to dry up using equipment in addition
The operation of standardization and precision can be realized Deng operation, the dependence to technical staff is reduced, it is to avoid because different personnel
Experience level is different and causes experimental result deviation occur.Convenient record operating process simultaneously, housekeeping operation data are IEM
The constantly improve offer data reference of detection technique.
Brief description of the drawings
A kind of Fig. 1, automation Inherited Metabolic Disorders detect the connection diagram of sample pretreatment equipment.
Wherein 1- central processor CPUs, 2- built-in storagies, 3- read only memory ROMs, 4- external storage devices, 5- mainboards,
6- temperature detections and management module, 7- reagent add modules, 8- sample-manipulating devices, 9- centrifugation control modules, 10- nitrogen supply (NS)s
Module, 11- epiphragma modules.
Embodiment
The utility model will be further described with specific embodiment below in conjunction with the accompanying drawings.
A kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment as shown in Figure 1, it includes central processing unit
CPU1, built-in storage 2, read only memory ROM 3, external storage device 4, mainboard 5, temperature detection and management module 6, reagent add
Plus module 7, sample-manipulating device 8, centrifugation control module 9, nitrogen supply (NS) module 10 and epiphragma module 11；
Wherein central processor CPU 1, built-in storage 2, read only memory ROM 3, temperature detection and management module 6, reagent
Add module 7, centrifugation control module 9, nitrogen supply (NS) module 10 are connected and led to by the circuit of mainboard 5 with epiphragma module 11
News；
External storage device 4 and sample-manipulating device 8 are connected by electrical wiring to mainboard 5, are communicated with central processor CPU 1,
Controlled sample-manipulating device 8 to operate by central processor CPU 1 and clamped sample to difference in functionality area, central processor CPU 1
Reagent add module 7, centrifugation control module 9, the and of nitrogen supply (NS) module 10 are controlled successively according to the feedback of the information of sample-manipulating device 8
Epiphragma module 11, reagent add module 7, centrifugation control module 9, nitrogen supply (NS) module 10 and epiphragma module 11 are run；Centre
Reason device CPU1 recorded system operation data in external storage device 4.
As the presently preferred embodiments, nitrogen supply (NS) module 10 includes nitrogen supply (NS) switch module and nitrogen blows speed control mould
Block, nitrogen supply (NS) light modules and nitrogen blow rate control module and connected by wire circuit.
As the presently preferred embodiments, epiphragma module 11 includes Teflon film epiphragma module 11, aluminium film capping module and position meter
Module is calculated, Teflon film epiphragma module 11, aluminium film capping module and the integrated epiphragma module 11 of position computation module, position calculate mould
Block is connected to displacement transducer, and displacement data is simultaneously transferred to position computation module by the displacement of displacement transducer detection film.
As the presently preferred embodiments, nitrogen supply (NS) module 10 is connected to nitrogen gas generator and nitrogen pot by wire circuit, just
Nitrogen gas generator is used in the case of often as source nitrogen, when detecting nitrogen gas generator failure or abnormal nitrogen supply (NS)
Nitrogen pot is automatically switched to, nitrogen gas generator is reused as nitrogen source after nitrogen gas generator or nitrogen supply (NS) recover normally
And supplement the consumption of nitrogen pot.
As the presently preferred embodiments, described external storage device includes a variety of mixing of hard disk, USB flash disk and immediate access device
Use.
Testing staff will detect that sample is positioned in 96 hole filters, and filter is put into automation Inherited Metabolic Disorders inspection
In the sample-manipulating device 8 of test sample product pre-processing device, start automation Inherited Metabolic Disorders detection sample pretreatment equipment, center
Processor CPU1 control sample-manipulating devices 8 clamp filter and filter are moved into reagent add module 7 and added to reagent
Plus the transmission addition methanol of module 7 is instructed, sample of the reagent add module 7 into filter adds containing the internal standard first and handled to center
Device CPU1 feedback methanol additions finish signal.Central processor CPU 1, which receives methanol addition and finished, starts timing after signal, 20min
Filter is clamped in centrifugation control module 9 by control chucking device afterwards, and sample is centrifuged.After centrifugation is finished, centrifugation control
Molding block 9 draws extract on polypropylene board and feeding back centrifugation and finish signal to central processor CPU 1, central processing unit
CPU1 controls sample-manipulating device 8 that polypropylene board is moved into nitrogen supply (NS) module 10, and is blown to the transmission of nitrogen supply (NS) module 10
Dry instruction.Nitrogen supply (NS) module 10 is started and is dried up polypropylene board using nitrogen, and feedback information is to central processor CPU 1,
Central processor CPU 1 controls sample-manipulating device 8 that polypropylene board is clamped into reagent add module 7 again.Center control reagent
Add module 7 adds hydrochloric acid n-butanol into polypropylene board, then controls sample-manipulating device 8 that polypropylene board is clamped into epiphragma mould
Block 11.Epiphragma module 11 is controlled by central processor CPU 1, covers Teflon film, the clamping of sample-manipulating device 8 polypropylene board is simultaneously
It is sent in insulating box, temperature detection detects with management module 6 and controls insulating box to be kept for 65 DEG C.Sample-manipulating device after 15min
Polypropylene board is sent to nitrogen supply (NS) module 10 by 8, and the Teflon film on polypropylene board is removed and blown by nitrogen supply (NS) module 10
It is dry.After drying, central processor CPU 1 controls sample-manipulating device 8 that polypropylene board is sent into reagent add module 7 and examination is controlled
Agent add module 7 adds acetonitrile to the sample on polypropylene board, then polypropylene board is sent into epiphragma module 11, epiphragma module 11 by
Central processor CPU 1 is controlled, and covers aluminium film film, and the clamping polypropylene board of sample-manipulating device 8 simultaneously send sample area on mass spectrograph.
Embodiment described above only expresses a kind of embodiment of the present utility model, and it describes more specific and detailed,
But therefore it can not be interpreted as the limitation to the utility model the scope of the claims.It should be pointed out that for the common of this area
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (5)
1. one kind automation Inherited Metabolic Disorders detection sample pretreatment equipment, it is characterised in that：It include central processor CPU,
Built-in storage, read only memory ROM, external storage device, mainboard, temperature detection and management module, reagent add module, sample
Chucking device, centrifugation control module, nitrogen supply (NS) module and epiphragma module；
Wherein central processor CPU, built-in storage, read only memory ROM, temperature detection and management module, reagent add module,
Centrifugation control module, nitrogen supply (NS) module are connected and communicated by the circuit of mainboard with epiphragma module；
External storage device and sample-manipulating device are connected by electrical wiring to mainboard, are communicated with central processor CPU, by centre
Reason device CPU control sample-manipulating devices are operated and clamp sample to difference in functionality area, and central processor CPU is set according to sample clamping
Standby feedback of the information controls reagent add module, centrifugation module, nitrogen supply (NS) module and epiphragma module, reagent addition mould successively
Block, centrifugation module, nitrogen supply (NS) module and the operation of epiphragma module；System operation data recorded outside by central processor CPU
In storage facilities.
2. a kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment according to claim 1, it is characterised in that：Nitrogen
Gas supply module includes nitrogen supply (NS) switch module and nitrogen blows rate control module, and nitrogen supply (NS) light modules and nitrogen blow
Dynamic rate control module is connected by wire circuit.
3. a kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment according to claim 1, it is characterised in that：Lid
Film module includes Teflon film epiphragma module, aluminium film capping module and position computation module, Teflon film epiphragma module, aluminium film lid
Face mould block and the integrated epiphragma module of position computation module, position computation module are connected to displacement transducer, displacement transducer detection
Displacement data is simultaneously transferred to position computation module by the displacement of film.
4. a kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment according to claim 1, it is characterised in that：Nitrogen
Gas supply module is connected to nitrogen gas generator or nitrogen pot by wire circuit.
5. a kind of automation Inherited Metabolic Disorders detection sample pretreatment equipment according to claim 1, it is characterised in that：Institute
The external storage device stated includes the one or more of hard disk, USB flash disk and immediate access device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720076287.5U CN206440976U (en) | 2017-01-22 | 2017-01-22 | One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720076287.5U CN206440976U (en) | 2017-01-22 | 2017-01-22 | One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206440976U true CN206440976U (en) | 2017-08-25 |
Family
ID=59635017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720076287.5U Active CN206440976U (en) | 2017-01-22 | 2017-01-22 | One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206440976U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110333312A (en) * | 2019-08-13 | 2019-10-15 | 暨南大学 | The device and method of organotin in a kind of extraction plastics |
-
2017
- 2017-01-22 CN CN201720076287.5U patent/CN206440976U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110333312A (en) * | 2019-08-13 | 2019-10-15 | 暨南大学 | The device and method of organotin in a kind of extraction plastics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109298115B (en) | Quantitative detection method for multiple metabolites in biological sample and metabolic chip | |
| US7469273B2 (en) | Multi-processor system verification circuitry | |
| CN102103535B (en) | Multicore processor, and system and method for debugging multicore processor | |
| JPH0416747B2 (en) | ||
| EP1437402B1 (en) | Nucleic acid extracting device | |
| CN105893261A (en) | Method for automatically testing stability of BMC (baseboard management controller) of server | |
| CN206440976U (en) | One kind automation Inherited Metabolic Disorders detection sample pretreatment equipment | |
| CN106701566A (en) | Nucleic acid extraction, amplification and detection integrated mechanical device | |
| CN114994509A (en) | Method for designing testability of chip | |
| CN101923096B (en) | Automatic westem blot analyzer | |
| CN106991026A (en) | It is a kind of to pass through the method that network carries out server memory Rank margin test in batches | |
| CN108195954A (en) | A kind of method that Full-automatic solid phase extraction-ultra-performance liquid chromatography quickly measures bongkrekic aicd content in food | |
| CN101545950B (en) | Scan control method and device | |
| CN101016564B (en) | Micro-array chip with forty-five gene locus for detecting mitochondria diabetes | |
| CN109239362B (en) | Application of lectin probe combination in identification of Qinling mountain golden monkey pregnancy based on urine protein sugar type | |
| CN111060807B (en) | High-speed integrated circuit test platform based on SoC and test method thereof | |
| CN100552636C (en) | A kind of outer diagnostic test interface of band and method of testing that is used for concurrent computational system | |
| CN203448193U (en) | Centrifugal machine for micro-column gel cards | |
| CN116777104A (en) | Preparation system, equipment, medium and terminal of ginsenoside Rg2 drug | |
| CN110938685A (en) | Gene detection probe set for neonatal hereditary metabolic disease and hemoglobinopathy and application thereof | |
| CN107703219B (en) | Evaluation GFP gene based on CILLC-MS transfects the method influenced on hPMSCs metabolism group | |
| JP7331617B2 (en) | Extraction device and extraction program | |
| CN114459872A (en) | Automatic processing method and device for conducting proteomics analysis on FFPE sample | |
| CN106770766A (en) | A kind of composition for detecting donkey hide derived components content in glue class Chinese medicine and its compound preparation, kit and its detection method | |
| CN114674943B (en) | Detection of 25 (OH) D in serum2And 25 (OH) D3Liquid chromatography mass spectrometry tandem detection method of (C) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200731 Address after: Unit 502-2, 5th floor, No.7, helix 4th Road, International Biological Island, Guangzhou, Guangdong 510000 Patentee after: Guangzhou Fuhui medical laboratory Co., Ltd Address before: 510000, C1 building, No. 11, Kaiyuan Avenue, Science City, Luogang District hi tech Industrial Development Zone, Guangdong, Guangzhou, Fourth Patentee before: GUANGZHOU FUHUI MEDICAL TECHNOLOGY Co.,Ltd. |