CN206438121U - A kind of medium culture device - Google Patents
A kind of medium culture device Download PDFInfo
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- CN206438121U CN206438121U CN201720101417.6U CN201720101417U CN206438121U CN 206438121 U CN206438121 U CN 206438121U CN 201720101417 U CN201720101417 U CN 201720101417U CN 206438121 U CN206438121 U CN 206438121U
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Abstract
The utility model provides a kind of medium culture device, including at least one culture vessel, light source, sensor, control system；Wherein, the culture vessel at least one side is transparent, and the light source and the sensor are connected with the control system respectively；The light source provides incident light and culture medium is irradiated；The sensor is acquired to the emergent light after irradiation culture medium, obtains outgoing optical signal；The control system includes analog-to-digital conversion module and signal processing module, wherein, the analog-to-digital conversion module is configured as the outgoing optical signal being converted into data signal, and the signal processing module is configured as the data signal being converted to color signal, to determine the colouring information of culture medium.The utility model effectively mitigates manpower burden, improves the stability and monitoring precision of culture environment, reduces toxigenic capacity.
Description
Technical field
The utility model is related to biological cell culture medium quality on-line monitoring field, particularly in closed environment
A kind of medium culture device based on color analysis.
Background technology
In biologic medical field, cell culture is very basic and core a techniqueflow., can by cell culture
Greatly to replicate amplified sample quantity, after separation, the cell amplified can be used for a small amount of sample of script can not
The multiple use of completion.
During cell culture, to ensure the reasonable growth of cell, it is necessary to nutritional ingredient needed for adding cell etc.
Many kinds of substance.To ensure the quality of cell culture, it is necessary to monitor change of the culture medium in incubation.Traditional culture medium training
Support and monitored to be artificial, personnel visually observe by culture, changing for culture medium internal composition is judged according to the change of culture medium color
Become, and then judge the quality condition of culture medium.The mode that naked eyes judge is thicker, it is common practice that：The culture of detection will be needed
Container takes out from culture environment (such as incubator)；By means such as range estimation, colorimetric cards, visually observe culture medium quality and
Cell growth environment, and judge；Next step is done everywhere to the culture medium in culture vessel according to the judgement of institute's observed case
Reason, or culture vessel is put back into culture environment continuation culture or next step operation is carried out.
However, because the catagen speed of culture medium in different incubations is different, culture medium need to be carried out constantly observation and
Check, cause to be frequently necessary to staff to be detected in the unsocial hours, cause greatly inconvenience and bear.Also,
Culture medium color detection is completed by human eye, in the case of by instruments such as colorimetric cards, and its testing result also varies with each individual,
More difficult progress quantization comparison, causes testing result error big, training quality is low.Led in the culture of individual cells or regional area
Domain, is generally also the artificial monitoring of the real-time incubation based on photomicrography, produces same test problems.
Utility model content
To solve problem of the prior art, the purpose of this utility model is to provide a kind of culture medium quality on-line monitoring system
System and method, by the culture vessel of light source radiation and seal, utilize color of the color sensor to the culture medium in culture vessel
Information is analyzed, to realize the real-time monitoring and monitoring of culture medium quality condition in cell cultivation process.
To solve the technical problem of prior art, the utility model provides a kind of medium culture device, including at least one
Individual culture vessel, and quality online monitoring system is carried out to the culture medium in the culture vessel, wherein, the monitoring system bag
Include light source, sensor, control system；Wherein, the light source and the sensor are connected with the control system respectively；The light
Source provides incident light and culture medium is irradiated；
The sensor is acquired to the emergent light after irradiation culture medium, obtains outgoing optical signal；The control system
System includes analog-to-digital conversion module and signal processing module, wherein, the analog-to-digital conversion module is configured as believing the emergent light
Number data signal is converted into, the signal processing module is configured as the data signal being converted to color signal, to determine
The colouring information of culture medium.Several culture vessels can be placed in culture apparatus described in the utility model to cultivate
Base, meanwhile, some culture vessels are monitored by quality online monitoring system.The culture vessel is closed environment, temperature in it,
Humidity and gas component can quantify control on demand.
Preferably, hole bracket is also included in the culture apparatus, the bottom of the culture vessel is transparent and positioned at there is hole
On support, the incident light of the light source is irradiated by the hole being on free support to culture medium, and the emergent light passes through
Hole on hole bracket reaches the sensor.
It is further preferable that the culture apparatus also includes mechanical arm, the mechanical arm moves and supports the monitoring system.
Preferably, the culture apparatus also includes support and mechanical arm, and the support is used to support the monitoring system, institute
Stating mechanical arm is used to move the monitoring system.
Preferably, the culture apparatus also includes support, and the monitoring system has sliding equipment and positioned at the support
On.
It is preferred that, for the ease of being put into incubator, the size (length, width and height difference) for the culture vessel of medium culture
Typically not greater than 200mm.
The utility model is acquired using sensor to emergent light, and emergent light is analyzed by control system, is obtained
Go out the real-time color information of culture medium.In this way, specifying in one section of incubation time, can according to the accuracy requirement of culture base monitoring
That a period of time at default multiple time points will carry out discretely obtaining culture medium colouring information, at the appointed time it can also connect in section
The colouring information of culture medium is obtained continuously, and the culture medium color at each time point obtains the mistake for including irradiation-collection-analysis
Journey.In this way, by the variation tendency for the colouring information for obtaining culture medium, then by the colouring information in obtained a period of time and initially
The standard value of collection income value and system calibrating is contrasted, and determines the further operation that will be taken culture medium.
The beneficial effects of the utility model are：
1. the monitoring of automation effectively mitigates manpower burden；
2. taking out the link that culture vessel is detected repeatedly by reducing, the disturbance repeatedly to culture medium is reduced, is carried
The high stability of culture environment；
3. culture medium quality is monitored automatically using sensor element and algorithm to Color-sensitive, by monitored results numeral
Change, improve monitoring precision, it is ensured that the reliability of monitoring process, reduce monitoring error；
4. reduce toxigenic capacity；
5. setting multiple culture vessels in culture apparatus, the culture efficiency of culture medium and making for monitoring system can be mentioned significantly
Use efficiency.
Brief description of the drawings
Fig. 1 is the utility model supervising device schematic diagram；
Fig. 2 is the utility model supervising device schematic perspective view；
Fig. 3 is light source schematic diagram；
Fig. 4 is sensor schematic；
Fig. 5 is that light source and sensor are arranged on heteropleural schematic diagram；
Fig. 6 is that light source and sensor are arranged on homonymy schematic diagram；
Fig. 7 is removable monitoring system schematic diagram；
Fig. 8 is the utility model monitoring method flow chart；
Fig. 9 is the utility model monitoring method process chart.
Embodiment：
In order that creation characteristic of the present utility model, technological means and reached purpose are readily apparent from understanding, below in conjunction with tool
The utility model is expanded on further in body embodiment.
Referring to Fig. 1-2, culture medium quality online monitoring system in a kind of culture vessel 11, including light source 13a/13b/13c,
Sensor 12a/12b/12c, control system 2；Wherein, the light source and the sensor carry out total with the control system respectively
Line is connected；The light source 13a/13b/13c provides incident light and culture medium 11a is irradiated, and culture medium 11a colors can be with it
Portion's change of component and change, the sensor to irradiation culture medium after emergent light be acquired, obtain outgoing optical signal；By
It is usually white light in incident light, white light carries whole colouring informations, after being irradiated to culture medium, and white light can be cultured base suction
A part is received, thus emergent light is to be cultured the part reflected light after base absorbs and scattered light, it is to carry color characteristic (light
Spectrum information) color of light, by analyzing the color of light, the color of culture medium now can be obtained.The control system 2
Including analog-to-digital conversion module and signal processing module, wherein, the analog-to-digital conversion module is configured as the outgoing optical signal
Data signal is converted into, the signal processing module is configured as the data signal being converted to color signal, to determine training
Support the colouring information of base.The light source 13a/13b/13c includes closed light source receptacle and is arranged in the closed light source receptacle
Light-emitting component, the light-emitting component is connected with the control system by light source connecting line, had outside the light source connecting line
Water-proof connector.The light-emitting component is incandescent lamp, fluorescent lamp, semiconductor diode photophore, laser, super luminescence diode
At least one of.
Referring to Fig. 3, the light source is irradiated by transmission or reflection to culture medium.13a, 13b, 13c are made by system
With light source, light source can mount to bottom, side or top.Light source 21 is included containing the airtight of the composition of at least one side transparent surface 22
Overall, light-emitting component 24 is installed on the inner side of transparent surface 22, and the line of itself and control system is realized by water-proof connector 23.
Referring to Fig. 4, the sensor 31 includes closed sensor container and is arranged in the closed sensor container
Color sensor element, the color sensor element 34 is installed on the inner side of transparent surface 32 and by sensor connecting line and institute
Control system connection is stated, the closed sensor container at least side is transparent, has waterproof outside the sensor connecting line
Connector 33.Sensor can be single element, also can be single original paper array composition.The color sensor element be CCD,
At least one of CMOS, photodiode, avalanche diode, photo resistance, photomultiplier.
For single culture vessel, due to there may be culture medium non-uniform mass, and single sensor inside it
The culture medium colouring information overlay area that can be gathered is limited, therefore multiple sensors of array can be set to single culture vessel,
Different zones to single culture vessel are monitored.Situation is obtained for the multizone color in single culture vessel, will be protected
The colouring information in each region is stayed, and according to the different monitoring accuracy requirement of system, using average value or extreme value (maximum or most
Small value) system information is monitored and alarmed.The position of light source 13 and sensor 12 sets and can be arranged as required to different
Side or homonymy, as seen in figs. 5-6.Shown in Fig. 5, light source 13 and sensor 12 are arranged on the both sides up and down of culture vessel, light source
13 send incident ray 141, and emergent light 142 is emitted as from the other end afterwards through culture medium 11, and are received by sensor 12, this
Utility model also includes the left and right sides that light source 13 and sensor 12 are arranged on to culture vessel, will not be described here.Such as Fig. 6 institutes
Show, light source 13 and sensor 12 are arranged on the same side of culture vessel, can be any sides in six faces of culture medium.Incident light
After 141 by reflection, emergent light 142 is received by sensor 12.
The culture medium quality online monitoring system is movably disposed in the culture apparatus.As shown in Figure 7.Due to
Culture vessel small volume, the usual volume of incubator is larger, for multiple culture vessels 11 in single incubator 16, can use
Two ways is monitored systems array, i.e., 1) set a set of monitoring system to carry out a pair for each container that need to be monitored
One color monitoring, or mechanical arm mobile monitoring system 2) is used, multiple culture vessels in same incubator are monitored.Figure
In, hole bracket 18 is used to support culture vessel 11, and hole bracket 18 can be fixedly connected with Z-direction support 172, can also
It is suspended on providing holes on culture upper box part, hole bracket 18, it is to avoid sensor, light source are blocked by support 18 in monitoring system.Branch
Frame 171 is used to support monitoring system, and support 171 is fixedly connected with Z-direction support 172.Mechanical arm can be as needed by monitoring
System is moved along support 171, makes the relevant position of hole bracket 18 of the monitoring system below culture vessel to be detected, light
The light that source is sent irradiates culture vessel by the hole of hole bracket 18, and the hole of emergent light through hole bracket 18 enters sensor.
When hole bracket 18 is individual layer, mechanical arm can not be also set, sliding equipment is directly set in monitoring system, control sliding equipment makes
Supervising device is slided along hole bracket 18 or support 171, reaches hole bracket relevant position and culture vessel is detected.There is hole
Support 18 can also set multilayer, and to support multilayer culture vessel, monitoring system can be placed on culture by mechanical arm as needed
Detected the lower section of hole bracket 18 below container.More simple structure, is not provided with support 171, directly by mechanical arm
Move and support monitoring system to the lower section of hole bracket 18, corresponding culture vessel is detected.
Many set control systems can be set in culture apparatus, its monitoring system faced have can simultaneously with multigroup control
Interface and disposal ability that (sampling) system is communicated, and every group of control (sampling) system (communicate with the line of monitoring system
Passage) it is independent, it does not interfere with each other, in data processing, is set by algorithm so as to realize in the communications, will will be often set control
(sampling) sets independent data processing function, realizes do not interfere with each other in data processing.
When culture vessel only have side it is transparent when, light requirement source and sensor are arranged on the same side, such case, and incident light is
White light with whole colouring informations, emergent light is to carry characteristic color light to be cultured the part reflected light after base absorbs and dissipate
Penetrate light.When culture vessel is all-transparent, sensor may be provided at culture vessel either side, such as be arranged on bottom, light source according to
Lighting demand may be provided at one or more of bottom/top/side position, and such case is incident when light source is in bottom
Light is the white light with whole colouring informations, and emergent light is to carry characteristic color light to be cultured the part reflected light after base absorbs
And scattered light, the irradiation of light source and the detection of detector can synchronously carry out, also can light source irradiation prior to detector detection.
The signal processing module also includes the color signal is averaged or integrated or smoothing processing.
Another embodiment of the utility model provides a kind of medium culture device, including at least one culture vessel, and right
Culture medium in the culture vessel carries out quality online monitoring system, wherein, the monitoring system include light source, sensor,
Control system；Wherein, the light source and the sensor are connected with the control system respectively；The light source provides incident light pair
Culture medium is irradiated；The sensor is acquired to the emergent light after irradiation culture medium, obtains outgoing optical signal；It is described
Control system include analog-to-digital conversion module and signal processing module, wherein, the analog-to-digital conversion module be configured as by it is described go out
Penetrate optical signal and be converted into data signal, the signal processing module is configured as the data signal being converted to color signal,
To determine the colouring information of culture medium.Several culture vessels can be placed in culture apparatus described in the utility model to cultivate
Culture medium, meanwhile, some culture vessels are monitored by quality online monitoring system.The culture vessel is closed environment, in it
Temperature, humidity and gas component can quantify control on demand.Control system and computing system can be put according to actual system design demand
Put in incubator outside culture vessel, i.e. space between incubator and culture vessel；Or outside incubator, to the defeated of control system
Go out the system that signal and alarm signal be monitored to be positioned over outside incubator, connected by line with incubator intraware
Connect.
The compartment of at least more than one is provided with the culture apparatus.For the ease of being put into incubator, for cultivating
The size (length, width and height difference) of the culture vessel of base culture is typically not greater than 200mm.
As Figure 8-9.The utility model also provides a kind of by being supervised according to the online monitoring system of one of claim
The method for surveying culture medium quality in culture vessel.Such as Fig. 8, the light that the utility model method is sent by light source, light penetration
Transparent interface enters culture medium, and culture medium is carried out to light after selective absorbing, then is received by sensor by culture medium suction
Remaining light after receipts, is analyzed the emergent light and incident light, so as to draw the state of culture medium.As shown in figure 9, specific
Processing and analysis process are：After control system receives outside control input, control system starts control measurement data, control
System to light source send instruction to drive light source to open, control system to color sensor send order-driven sensor sensing/
The color signal for the light that reception/receiving is detected, sensor believes color the signal transduction to control system, control system
Number quantity be to light color sample number of times judge that the foundation of judgement can be according to the outside control input most started
Data, if reaching the number of times requirement of outside input, terminate sampling, control system send instruction close light source and to collection
Data handled after feed back final numerical value.If not reaching the number of times requirement of outside input, continue to color sensor
Send instruction and require that sensor continues the light color signal that sensing/reception/receiving is detected.
The utility model, to outgoing light collection, is analyzed emergent light by control system using sensor, draws training
Support the real-time color information of base.In this way, specifying in one section of incubation time, can be by according to the accuracy requirement of culture base monitoring
A period of time at default multiple time points carry out discretely obtaining culture medium colouring information, can also at the appointed time in section continuously
The colouring information of culture medium is obtained, the culture medium color at each time point obtains the process for including irradiation-collection-analysis.
In this way, by the variation tendency for the colouring information for obtaining culture medium, then by the colouring information in obtained a period of time with initially adopting
The standard value of collection income value and system calibrating is contrasted, and determines the further operation that will be taken culture medium.System calibrating
Standard value, for example can be that demarcation is good, normal, failure within the scope of some color value.
Entered using the digitized color sensor that data signal exported is converted to after directly color signal is gathered
Row sensing acquisition.Color samples particularized below and analysis embodiment.
Embodiment：
When incident light is white light, such as use RGB per Color Channel for 8bit depth, then can be by the calibration of white light color signal
(256,256,256)；
When the qualified color of composition of used culture medium indicator feature is red, when composition failure color is colourless.
Now, when composition is qualified, solution is far above the assimilation ratio to feux rouges to the light absorbs ratio of other wavelength in addition to red light wavelength,
Therefore solution takes on a red color；When composition fails, the light of all colours is absorbed close to homogenous proportion, thus particular color will not be presented.
When using qualified culture medium, light source sends white light (RGB 256:256:256) irradiation is penetrated after culture medium, is inhaled
Receive remaining light shown in red (example, RGB 200:50:50), due to many reasons such as light-source system, ambient lightings, it can survey
Noise is introduced in value, i.e. gained measured value is in actual value (RGB 200:50:50) nearby fluctuate (for example：210:60:60–
190:45:45) in this regard, taking the mode of data processing after multiple repairing weld, the sampling actual value on single time point is obtained, for
The data processing method of multiple repairing weld for example can be：Using simple average --- i.e. by n times sampled value sum after divided by N, obtain
Obtain average value；Using further digital filtering, the noise of specific frequency (such as mains frequency 50Hz/60Hz) is filtered
Remove.
After culture medium fails；Culture medium indicator loses selection absorption characteristic, and culture medium now is to all spectrum
Light uniform pickup, i.e. light source send white light (RGB 256:256:256) irradiation is penetrated after culture medium, is absorbed remaining light and is shown
For colourless (example, RGB 60:60:60), likewise, by this data by multiple repairing weld and the method for processing, obtaining the single time
True colors signal on point.And pass through data processing as described above, to draw the state of culture medium, and it is next step decision-making
Reference is provided.
The utility model device and method is applied to the culture medium that color can change and change with its internal composition, culture medium
For detected object, the restriction for describing not to the utility model device and sending out method to culture medium.
General principle of the present utility model, principal character and advantage of the present utility model has been shown and described above.One's own profession
The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification
Simply illustrate principle of the present utility model, the utility model can also on the premise of the utility model spirit and scope are not departed from
There are various changes and modifications, these changes and improvements are both fallen within the range of claimed the utility model.The utility model will
Protection domain is asked to be defined by appended claims and its equivalent.
Claims (6)
1. a kind of medium culture device, it is characterised in that including at least one culture vessel, and in the culture vessel
Culture medium carries out quality online monitoring system, wherein,
The culture vessel at least one side is transparent；
The monitoring system includes light source, sensor, control system；Wherein, the light source and the sensor respectively with it is described
Control system is connected；
The light source provides incident light and culture medium is irradiated；
The sensor is acquired to the emergent light after irradiation culture medium, obtains outgoing optical signal；
The control system includes analog-to-digital conversion module and signal processing module, wherein, the analog-to-digital conversion module is configured as
The outgoing optical signal is converted into data signal, the signal processing module is configured as the data signal being converted to face
Chrominance signal, to determine the colouring information of culture medium.
2. culture apparatus according to claim 1, it is characterised in that also include hole bracket, institute in the culture apparatus
The bottom for stating culture vessel is transparent and on the hole bracket, and the incident light of the light source passes through the hole pair on the hole bracket
Culture medium is irradiated, and the emergent light reaches the sensor by the hole on hole bracket.
3. culture apparatus according to claim 2, it is characterised in that also including mechanical arm, the mechanical arm is moved and propped up
Support the monitoring system.
4. culture apparatus according to claim 2, it is characterised in that also including support and mechanical arm, the support is used for
The monitoring system is supported, the mechanical arm is used to move the monitoring system.
5. culture apparatus according to claim 2, it is characterised in that also including support, the monitoring system, which has, to be slided
Mechanism is simultaneously located on the support.
6. culture apparatus according to claim 2, it is characterised in that the size (length, width and height difference) of the culture vessel is no
More than 200mm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644964A (en) * | 2017-01-25 | 2017-05-10 | 上海宸安生物科技有限公司 | Online monitoring system and method for quality of culture medium in culture vessel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106644964A (en) * | 2017-01-25 | 2017-05-10 | 上海宸安生物科技有限公司 | Online monitoring system and method for quality of culture medium in culture vessel |
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