CN206411010U - Carry the ATP fluoroscopic examination swabs of sample sipper tube - Google Patents
Carry the ATP fluoroscopic examination swabs of sample sipper tube Download PDFInfo
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- CN206411010U CN206411010U CN201720021890.3U CN201720021890U CN206411010U CN 206411010 U CN206411010 U CN 206411010U CN 201720021890 U CN201720021890 U CN 201720021890U CN 206411010 U CN206411010 U CN 206411010U
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Abstract
The utility model belongs to field of biological detection, is related to the swab of detection, and in particular to a kind of ATP fluoroscopic examination swabs for carrying sample sipper tube.Including base, the base upwards with elastomeric cap pipe nested encryptions, be connected with sample sipper tube downwards, an outside reaction tube nested again.Having in the middle of the base on through hole, the sample sipper tube diverse location has scale to show corresponding collection volume.Further, sampled by inserting the hollow quarter butt of a headband cotton balls in the openend of sample sipper tube for surface.The utility model can realize the sampling of different volumes water sample, also simplify the swab assembling with water sampler and cotton swab.
Description
Technical field
The utility model belongs to field of biological detection, is related to the swab of detection, and in particular to one kind carries sample sipper tube
ATP fluoroscopic examination swabs.
Background technology
ATP, i.e. atriphos（Adenosine Triphosphate）, it is prevalent in all living things intracellular.Profit
The principle lighted with ATP and fluorescein-luciferase reaction, can delicately detect the microbe quantity including bacterium
Amount, has a wide range of applications in food processing enterprises and hospital, dining room, supermarket and other manufacturings, can quickly determine
Clean effect and sanitary condition of surface and water sample etc..
In addition to ATP fluorescence detectors, in actually detected in order that with and it is easy to operate, in addition it is also necessary to adopted with swab
Sample.It is different according to the object of sampling in current ATP fluoroscopic examination swab structures, mainly there are 2 major classes.One is to be used for collection liquid
The swab of body, mainly water sample.The Hygiena companies in such as U.S. of some producers, using the plastics with certain structure of customization
Rod, adsorbs certain water sample to be acquired by surface tension, easy to use, and major defect can not realize different volumes
Water sampling.In addition, for the water sample of differential tension, sampling volume might have certain error.More also need user
After the liquid that certain volume oneself is gathered using pipettor, it is added in reaction tube.Although this can gather different volumes
Water sample, but user is inconvenient for use, also increases the possibility that external pipettor brings additional contamination.Two be to be used to gather body surface
The swab with cotton swab.This kind of swab typically has the sticking plaster of cotton balls using a hollow headband.Due to being adopted with water sample
The structure of storage is different, and cotton stick swab need to be individually designed in fit on.
Utility model content
In view of the deficienciess of the prior art, technical problem to be solved in the utility model is to provide a kind of while suitable
For water sample and the ATP fluoroscopic examination swabs for showing sampling.The ATP fluoroscopic examinations swab can be used to gather different volumes liquid-like
Originally it is, simple to operate, after simple combination, it can be used for sample surfaces sampling.
The utility model, which is adopted the following technical scheme that, to be achieved：
A kind of ATP fluoroscopic examination swabs for carrying sample sipper tube, including base, the base top are nested with elastomeric cap pipe
Connection, bottom is connected with sample sipper tube, has a reaction tube outside the sample sipper tube, be connected with base bottom nested；It is described
There is through hole to connect elastomeric cap pipe and sample sipper tube in the middle of base, the surface of the through hole is set between hydrophobic surface or through hole
Having on isolating valve, the body of the sample sipper tube has scale.
Sample sipper tube is made up of elastomeric material, has scale to show corresponding sampling volume, bottom on body diverse location
There is opening to be used to gather liquid-like.
When gathering liquid sample, the reaction tube is pulled up, extruding sample sipper tube top with finger excludes air, then
Liquid is immersed into sample sipper tube openend, come up finger, when liquid reaches the volume markings to be gathered, by sample sipper tube
Liquid is taken out, then the liquid in sample sipper tube is squeezed into the reaction tube, sampling is completed.
Fluorescein-luciferase reaction solution is loaded with the elastomeric cap pipe.Described elastomeric cap pipe uses soft material
Material, can bear extruding and volume diminishes so that elastomeric cap inner reaction tube liquid is by the through hole of the base, and by described
Sample sipper tube, finally falls in reaction tube.
It is above-mentioned carry the ATP fluoroscopic examination swabs of sample sipper tube on the basis of, by under described sample sipper tube
A hollow quarter butt is inserted in the openend in portion, and the described hollow quarter butt other end is with cotton balls, and there is fluid hole centre, can further changed
Enter for the ATP fluoroscopic examination swabs with cotton swab, wipe and sample for sample surfaces.
ATP detections swab of the present utility model has following technological merit compared to prior art：
It is not required to add-on device, it is possible to realize the liquid sample collection of different volumes, add ATP fluoroscopic examination swabs
Flexibility；Sampling volume is determined by the inner volume of elastic tube, quantitative not influenceed by surface tension；And further can simply transform
For the swab with cotton swab, the detection of pollutant on body surface.
Brief description of the drawings
Fig. 1, carries the structural representation of the ATP fluoroscopic examination swabs of sample sipper tube.
Fig. 2, the partial schematic diagram of the hollow quarter butt of sample sipper tube connect band cotton balls.
In figure, 1- elastomeric cap pipes, 2- reaction solutions, 3- bases, 31- through holes, 4- reaction tubes, 5- sample sipper tubes, 51- scales,
The hollow quarter butts of 6-, 61- fluid holes, 62- cotton balls.
Embodiment
As shown in figure 1, a kind of ATP fluoroscopic examination swabs for carrying sample sipper tube, including base 3, the base top with
The nested encryptions of elastomeric cap pipe 1, bottom is connected with sample sipper tube 5, there is a reaction tube 4 outside the sample sipper tube 5, with base 3
Bottom nested is connected；There is through hole 31 to connect elastomeric cap pipe 1 and sample sipper tube 5 in the middle of the base, the surface of the through hole 31 is
Being provided between hydrophobic surface or through hole on isolating valve, the body of the sample sipper tube has scale 51.
Before use, holding fluorescein-luciferase reaction solution 2 into elastomeric cap pipe 1.
The material of described base 3 can be that the material of hydrophobic plastic, such as polytetrafluoroethylene (PTFE), or described base 3 is it
Its material, but dimethyl silicone polymer is passed through on the surface of through hole 31（) etc. PDMS processing is hydrophobic surface, or in through hole 31
Middle setting isolating valve, the valve can be opened when necessary allows reaction solution 2 to flow through.
Described elastomeric cap pipe 1 uses soft plastics, and such as transparent silica gel or igelite can be born
Extruding and internal pressure becomes big so that fluorescein-luciferase reaction solution 2 proceeds to reaction by the through hole 31 of the base 3
In pipe 4.
Through hole 31 in base 3 is connected with the sample sipper tube 5 of bottom in base 3.
There is scale 51 on sample sipper tube body, totally 10 graduation marks, each scale represents 10 μ L or other volumes.
The assembling of the utility model swab and the course of work are as described below：
Swab of the present utility model before assembling, is assembled after the sterilized sterilizing of all building blocks.
During for water sampling：Sample sipper tube 5 is inserted to the bottom of base 3 first, then elastomeric cap pipe 1 is inverted and opened
Mouth end upward, is added after quantitative reaction solution 2, and base 3 and the component of sample sipper tube 5 are inverted, the upper end insertion elastomeric cap of base 3
In pipe 1, afterwards turn over.The quantitative rear enclosure of lysate 6 is added in reaction tube 4 again in the lower end of base 3.
When being gathered for surface sample：Sample sipper tube 5 is inserted to the bottom of base 3 first, then elastomeric cap pipe 1 is inverted
Openend upward, is added after quantitative reaction solution 2, and base 3 and the component of sample sipper tube 5 are inverted, the upper end insertion lid pipe of base 3
In 1, afterwards turn over.Hollow quarter butt 6 with cotton balls 62 is inserted to the bottom of sample sipper tube 5 again, and soaked in lysate, finally
Reaction tube 4 is loaded on to the lower end of base 3.
During sampling：
During for water sampling：ATP detection swabs are taken out first.Base 3 is pinched on the other hand, reaction tube 4 is caught on the other hand, and
Reaction tube 4 is pulled up.Thumb and forefinger catch the top of sample sipper tube 5, and one pinches the collection that a pine carries out water sample, passes through graduation mark
51 quantify gathered volume of water sample, such as 100 μ L.Then the water sample collected is instilled in reaction tube 4, then by reaction tube 4
It is reinstalled onto the bottom of base 3.If the thalline in water sample is too many, 2-10 times can be carried out and diluted.Concrete operation method（With 10 times
Exemplified by dilution）：Ultra-pure water is taken to the 9th article of graduation mark, then water sampling is to the 10th article of graduation mark.
When being gathered for surface sample：ATP detection swabs are taken out first.Base 3 is pinched on the other hand, reaction tube 4 is caught on the other hand,
And pull up reaction tube 4.Then surface to be measured is wiped using the cotton balls 53 on hollow quarter butt（According to sampling face set in advance
Product, such as 10cm × 10cm is wiped）, complete after sampling, then reaction tube 4 is reinstalled onto the bottom of base 3.
During detection：
It is after the completion of water sample sampling that whole device is vertical, the side wall of elastomeric cap pipe 1 is extruded with forefinger and thumb（If logical
Valve is provided with hole 31, first valve is opened before step operation herein）, under pressure, reaction solution 2 overcomes surface tension to pass through cylinder
Through hole 31, the runner along sample sipper tube 5 is toward flowing down, into reaction tube 4, until all reaction solutions 2 all enter reaction tube 4
Just stop the side wall of extruding elastomeric cap pipe 1 afterwards.Last whipping causes all liq to return to behind the bottom of reaction tube 4, will wipe rapidly
Son is put into ATP fluorescence detectors and tested.
After the completion of cotton balls sampling that whole swab is vertical, operation is essentially identical with water sampling above, with forefinger and big thumb
Refer to the side wall of extruding elastomeric cap pipe 1（If being provided with valve in through hole 31, first valve is opened before step operation herein）, in pressure effect
Under, reaction solution 2 overcomes surface tension by cylindrical hole 31, and the runner along sample sipper tube 5 is toward flowing down, by fluid hole 61
Afterwards, cotton balls is flowed through, is finally entered in reaction tube 4, extruding elasticity is just stopped after reaction tube 4 until all reaction solutions 2 are all entered
The side wall of lid pipe 1.Last whipping causes all liq to return to behind the bottom of reaction tube 4, and swab is put into ATP fluoroscopic examinations rapidly
Tested in instrument.
It is described above, only the utility model preferred embodiment, any one skilled in the art is at this
In scope disclosed in utility model, equivalent substitution or change are subject to according to the technical solution of the utility model and its design, all
Belong to protection domain of the present utility model.
Claims (6)
1. a kind of ATP fluoroscopic examination swabs for carrying sample sipper tube, it is characterised in that including base, the base top and bullet
Property lid pipe nested encryptions, bottom is connected with sample sipper tube, there is a reaction tube outside the sample sipper tube, reaction tube with base
Portion's nested encryptions；There is through hole to connect elastomeric cap pipe and sample sipper tube in the middle of the base, the surface of the through hole is hydrophobic surface
Or isolating valve, is provided between through hole.
2. ATP fluoroscopic examinations swab according to claim 1, it is characterised in that have quarter on the body of the sample sipper tube
Degree.
3. ATP fluoroscopic examinations swab according to claim 1, it is characterised in that be loaded with fluorescence in the elastomeric cap pipe
Element-luciferase reaction liquid.
4. ATP fluoroscopic examinations swab according to claim 1, it is characterised in that the opening of described sample sipper tube bottom
End is inserted with a hollow quarter butt, and the described hollow quarter butt other end is with cotton balls, and there is fluid hole centre.
5. ATP fluoroscopic examinations swab according to claim 1, it is characterised in that the material of described base is hydrophobic modeling
Material, or the base through-hole surfaces pass through dimethyl silicone polymer processing.
6. ATP fluoroscopic examinations swab according to claim 1, it is characterised in that described elastomeric cap tubing matter is silica gel
Or polyvinyl chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720021890.3U CN206411010U (en) | 2017-01-10 | 2017-01-10 | Carry the ATP fluoroscopic examination swabs of sample sipper tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720021890.3U CN206411010U (en) | 2017-01-10 | 2017-01-10 | Carry the ATP fluoroscopic examination swabs of sample sipper tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206411010U true CN206411010U (en) | 2017-08-15 |
Family
ID=59553541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720021890.3U Active CN206411010U (en) | 2017-01-10 | 2017-01-10 | Carry the ATP fluoroscopic examination swabs of sample sipper tube |
Country Status (1)
| Country | Link |
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| CN (1) | CN206411010U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107961115A (en) * | 2017-12-08 | 2018-04-27 | 柳州市妇幼保健院 | Anti-pollution sterile swab |
| CN111999368A (en) * | 2020-08-05 | 2020-11-27 | 杭州电子科技大学 | Method for identifying plant anthraquinone pigment |
| CN112877191A (en) * | 2021-02-22 | 2021-06-01 | 西安交通大学 | Anti-pollution consumable material and method for performing CRISPR molecular diagnosis by using same |
-
2017
- 2017-01-10 CN CN201720021890.3U patent/CN206411010U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107961115A (en) * | 2017-12-08 | 2018-04-27 | 柳州市妇幼保健院 | Anti-pollution sterile swab |
| CN111999368A (en) * | 2020-08-05 | 2020-11-27 | 杭州电子科技大学 | Method for identifying plant anthraquinone pigment |
| CN111999368B (en) * | 2020-08-05 | 2022-09-09 | 杭州电子科技大学 | Method for identifying plant anthraquinone pigment |
| CN112877191A (en) * | 2021-02-22 | 2021-06-01 | 西安交通大学 | Anti-pollution consumable material and method for performing CRISPR molecular diagnosis by using same |
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