CN206382026U - A kind of cellular fat particle detections chip - Google Patents
A kind of cellular fat particle detections chip Download PDFInfo
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- CN206382026U CN206382026U CN201621475665.9U CN201621475665U CN206382026U CN 206382026 U CN206382026 U CN 206382026U CN 201621475665 U CN201621475665 U CN 201621475665U CN 206382026 U CN206382026 U CN 206382026U
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- microchannel
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Abstract
The utility model belongs to micro-current controlled cell detection chip technical field, more particularly to a kind of cellular fat particle detections chip.The utility model includes the chip basal body of sheet, and chip basal body is internally provided with main channel, and one end of main channel is provided with injection port；The side of main channel is provided with and communicated with detection reagent passage, and the end of detection reagent passage is provided with reagent inlet；The opposite side of main channel, which is sequentially communicated, is provided with three groups of microchannel collectors, and corresponding with three groups of microchannel collectors respectively conical connecting tube, biased sample passage, cell detection passage and cell collection channel；A plurality of internal diameter identical microchannel is set side by side with every group of microchannel collector；The bottom surface of the conical connecting tube and microchannel header in communication, the drift angle of conical connecting tube are connected with biased sample passage；The end of the cell collection channel extends to the outside of chip basal body.The utility model further relates to a kind of detection reagent for above-mentioned detection chip.
Description
Technical field
The utility model belongs to micro-current controlled cell detection chip technical field, more particularly to a kind of cellular fat particle detections
Chip.
Background technology
Cell detection chip is a kind of biochip technology using cell as research object, and it is for adaptation gene, divided
In period of the day from 11 p.m. to 1 a.m generation, is for exploring the emerging technology that life science demand is also produced.It is thin that cell detection chip technology had both maintained tradition research
The advantage of born of the same parents' method, the features such as high flux, large sample and quick obtaining cellular informatics are met again.Available for research specific gene
And the correlation between marking protein and disease, it is diagnosis, AD-targeted drugs screening, cellular localization for disease, anti-
Have a wide range of applications in terms of body drug screening.
The polytype cells, blood disease such as granulocytic systen, red blood cell system, megakaryocytic system are included in hematopoietic cell
Disease diagnosis needs to identify cell type.It is used to aid in hematopoetic cell types to differentiate using cellular fat granules stain, grain
Cell system, myeloblast is generally negative reaction, and what is had a small amount of positive particle can occurs, progranulocyte and following
Positive reaction is presented in each phase cell, and gradually strengthens with the ripe positive reaction of cell, particle increase, neutrophil leucocyte
Particle is more uniform, and eosinophil particle is thick, colour cast brown, granular center coloring is shallow and edge color depth, basophilla
Granulocyte, which is run, is presented negative or positive reaction, and positive particle is not of uniform size.Monocyte system, monoblast is generally the moon
Property reaction, weakly positive reaction, the distribution of particle small and dispersed is presented in juvenile cell core and monocyte；Other cells, lymphocyte,
Negative reaction is presented in red blood cell, erythroneocytosis, megacaryocyte, blood platelet, and it is anti-that weakly positive can be presented in desmacyte, macrophage
Should.
Traditional granulocyte lipochondrion is determined using index cell smear first, after cell is fixed, and is utilized
Fatty dyestuff is dyed to cellular fat particle, finally judges intracellular lipochondrion by micro- sem observation.Due to marrow,
The ratio of red blood cell and quantity of leucocyte about 2000 in blood：1, the easy interference measurement of conventional method red blood cell in continuous mode
As a result, cause testing result error larger.Therefore need to research and develop a kind of detection chip that can be reduced and determine interference.
Utility model content
The utility model provides a kind of cellular fat particle detections core to solve technical problem present in known technology
Piece.
The utility model is adopted the technical scheme that to solve technical problem present in known technology：A kind of cytolipin
Fat particle detections chip includes the chip basal body of sheet, and chip basal body is internally provided with main channel, and one end of main channel is provided with
Injection port, the injection port extends to the outside of chip basal body；The side of main channel is provided with and communicated with detection reagent passage, detection
The end of reagent passage is provided with reagent inlet, and the reagent inlet extends to the outside of chip basal body；Main channel it is another
Side, which is sequentially communicated, is provided with three groups of microchannel collectors, and cone connection corresponding with three groups of microchannel collectors respectively
Pipe, biased sample passage, cell detection passage and cell collection channel；It is set side by side with every group of microchannel collector in a plurality of
Footpath identical microchannel；The bottom surface of the conical connecting tube and microchannel header in communication, the drift angle of conical connecting tube is with mixing
Close sample channel connection；The end of the cell collection channel extends to the outside of chip basal body.
Advantage of the present utility model and good effect are：This chip volume is small, and amount of samples is few, quick, detection efficiency is high,
The interference in determining can be reduced, it is adaptable to intellectualized detection hematopoietic cell.
Preferably：Three groups of microchannel collectors are respectively that the first microchannel collector, the second microchannel collector and the 3rd are micro-
Passage collector；Microchannel quantity in first microchannel collector is 20-60, and the internal diameter of microchannel is 16-40 μm；Second is micro- logical
Microchannel quantity in road collector is 40-80, and the internal diameter of microchannel is 12-14 μm；Microchannel in 3rd microchannel collector
Quantity is 60-100, and the internal diameter of microchannel is 6-10 μm.
Preferably：The main channel is cylindrical passage or square column type tubular conduit.
Preferably：The detection reagent passage is cylindrical passage or square column type tubular conduit.
Preferably：The cell detection passage is tetragonal body structure.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
In figure：1st, main channel；2nd, detection reagent passage；3rd, microchannel collector；31st, the first microchannel collector；32nd, second is micro-
Passage collector；33rd, the 3rd microchannel collector；4th, conical connecting tube；5th, biased sample passage；6th, cell detection passage；7th, it is thin
Born of the same parents' collection channel；8th, chip basal body.
Embodiment
For invention, features and effects of the present utility model can be further appreciated that, following examples are hereby enumerated specifically
It is bright as follows：
Fig. 1 is referred to, the utility model includes the chip basal body 8 of sheet, and chip basal body 8 is internally provided with main channel
1, one end of main channel 1 is provided with injection port, and the injection port extends to the outside of chip basal body 8；The side connection of main channel 1
Detection reagent passage 2 is provided with, the end of detection reagent passage 2 is provided with reagent inlet, and the reagent inlet is extended to
The outside of chip basal body 8；The opposite side of main channel 1, which is sequentially communicated, is provided with three groups of microchannel collectors 3, and micro- with three groups respectively
The corresponding conical connecting tube 4 of passage collector 3, biased sample passage 5, cell detection passage 6 and cell collection channel 7；
A plurality of internal diameter identical microchannel is set side by side with every group of microchannel collector 3；The bottom surface of the conical connecting tube 4 leads to micro-
Road collector 3 is connected, and the drift angle of conical connecting tube 4 is connected with biased sample passage 5；Prolong the end of the cell collection channel 7
Extend the outside of chip basal body 8.
Three groups of microchannel collectors 3 respectively the first microchannel collector 31, the second microchannel collector 32 and the 3rd are micro- logical
Road collector 33；Microchannel quantity in first microchannel collector 31 is 20-60, and the internal diameter of microchannel is 16-40 μm；Second is micro-
Microchannel quantity in passage collector 32 is 40-80, and the internal diameter of microchannel is 12-14 μm；In 3rd microchannel collector 33
Microchannel quantity is 60-100, and the internal diameter of microchannel is 6-10 μm.
In the present embodiment, the main channel 1 is cylindrical passage or square column type tubular conduit.
In the present embodiment, the detection reagent passage 2 is cylindrical passage or square column type tubular conduit.
In the present embodiment, the cell detection passage 6 is tetragonal body structure.
Detection reagent for above-mentioned detection chip includes solution A, B solution, C solution and solution D.
The preparation method of wherein solution A is：0.5mg-1mg the Sudan's B reagents are taken, are dissolved in 100ml absolute ethyl alcohols, then
80 DEG C of heating and simultaneously stirring are extremely dissolved for 48 hours.
The preparation method of B solution is：Take 7.5mg methacrylic acid methoxypolyethylene glycol esters (Poly (ethylene
Glycol) methyl ether methacrylate) be dissolved in 10ml-100ml dichloromethane, then heat and shake
In the case of, stir 10-180 minutes, to whole dissolvings.
The preparation method of C solution is：Above-mentioned solution A and B solution are mixed in equal volume, then in the situation for heating and shaking
Under, mix two kinds of solution, mixed solution add isometric temperature be 80 DEG C, the 10mmol/L phosphate that pH value is 7.4
In cushioning liquid (PBS), continue heating stirring 0.5-4 hours, wherein inorganic matter molecular ratios is changed, finally centrifuge
0.5-5 hours, remove sediment fraction.
Solution D is the 10mmol/L phosphate buffers (PBS) that pH value is 7.4.
The method detected using above-mentioned detection chip and detection reagent is comprised the following steps:
1st, cell suspension to be detected and solution C are taken using volume ratio as 1：(5-500) ratio is mixed, and is fully mixed, at 27 DEG C
Stirring mixing 10-30 minutes, is set to E solution by this mixed liquor.
2nd, solution D is injected into chip by the detection reagent passage 2 on chip first, flow velocity is 1ml-100ml/h.
3rd, the injection port of chip main channel 1 is opened, E solution is entered main channel 1, injection flow velocity is 0.1ml-10ml/h.
4th, the cell in the cell storage pond of cell detection passage 6 is collected, CCD collection cell images, observation includes blue-black
The cell of coloured particles is intracellular lipochondrion positive cell.
5th, whether there is blue-black coloured particles, the quantity of particle, big I by intracellular, auxiliary examination cell type and thin
The biological aspect of born of the same parents.
Claims (5)
1. a kind of cellular fat particle detections chip, it is characterised in that：In chip basal body (8) including sheet, chip basal body (8)
Portion is provided with main channel (1), and the one end of main channel (1) is provided with injection port, and the injection port extends to the outer of chip basal body (8)
Portion；The side of main channel (1) is provided with and communicated with detection reagent passage (2), and the end of detection reagent passage (2) is provided with reagent note
Entrance, the reagent inlet extends to the outside of chip basal body (8)；The opposite side of main channel (1), which is sequentially communicated, is provided with three
Group microchannel collector (3), and corresponding with three groups of microchannel collectors (3) respectively conical connecting tube (4), biased sample lead to
Road (5), cell detection passage (6) and cell collection channel (7)；It is set side by side with every group of microchannel collector (3) in a plurality of
Footpath identical microchannel；The bottom surface of the conical connecting tube (4) is connected with microchannel collector (3), conical connecting tube (4)
Drift angle is connected with biased sample passage (5)；The end of the cell collection channel (7) extends to the outside of chip basal body (8).
2. cellular fat particle detections chip as claimed in claim 1, it is characterised in that：Three groups of microchannel collectors (3)
Respectively the first microchannel collector (31), the second microchannel collector (32) and the 3rd microchannel collector (33)；First microchannel collection
It is 20-60 to manage the microchannel quantity in (31), and the internal diameter of microchannel is 16-40 μm；It is micro- in second microchannel collector (32)
Number of channels is 40-80, and the internal diameter of microchannel is 12-14 μm；Microchannel quantity in 3rd microchannel collector (33) is 60-
100, the internal diameter of microchannel is 6-10 μm.
3. cellular fat particle detections chip as claimed in claim 2, it is characterised in that：The main channel (1) is cylinder
Tubular conduit or square column type tubular conduit.
4. cellular fat particle detections chip as claimed in claim 2, it is characterised in that：The detection reagent passage (2) is
Cylindrical passage or square column type tubular conduit.
5. cellular fat particle detections chip as claimed in claim 2, it is characterised in that：The cell detection passage (6) is
Tetragonal body structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621475665.9U CN206382026U (en) | 2016-12-30 | 2016-12-30 | A kind of cellular fat particle detections chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621475665.9U CN206382026U (en) | 2016-12-30 | 2016-12-30 | A kind of cellular fat particle detections chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206382026U true CN206382026U (en) | 2017-08-08 |
Family
ID=59491416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621475665.9U Withdrawn - After Issue CN206382026U (en) | 2016-12-30 | 2016-12-30 | A kind of cellular fat particle detections chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206382026U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732839A (en) * | 2016-12-30 | 2017-05-31 | 天津禄浩科技股份有限公司 | A kind of cellular fat particle detections chip and its detection reagent |
| CN107907521A (en) * | 2017-12-13 | 2018-04-13 | 广东顺德墨赛生物科技有限公司 | micro-fluidic detection system |
-
2016
- 2016-12-30 CN CN201621475665.9U patent/CN206382026U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732839A (en) * | 2016-12-30 | 2017-05-31 | 天津禄浩科技股份有限公司 | A kind of cellular fat particle detections chip and its detection reagent |
| CN106732839B (en) * | 2016-12-30 | 2022-04-26 | 天津禄浩科技股份有限公司 | Cell fat particle detection chip and detection reagent thereof |
| CN107907521A (en) * | 2017-12-13 | 2018-04-13 | 广东顺德墨赛生物科技有限公司 | micro-fluidic detection system |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170808 Effective date of abandoning: 20220426 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20170808 Effective date of abandoning: 20220426 |
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |