CN206378647U - A kind of beam shaping modulation piece of focal beam spot Fast Convergent - Google Patents
A kind of beam shaping modulation piece of focal beam spot Fast Convergent Download PDFInfo
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- CN206378647U CN206378647U CN201720029216.XU CN201720029216U CN206378647U CN 206378647 U CN206378647 U CN 206378647U CN 201720029216 U CN201720029216 U CN 201720029216U CN 206378647 U CN206378647 U CN 206378647U
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- amplitude modulation
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Abstract
Piece is modulated the utility model discloses a kind of beam shaping of focal beam spot Fast Convergent.The beam shaping modulation piece is optical filter, its light intensity transmitance distribution center is symmetrical, the everywhere light intensity transmitance of its radial direction is calculated using Modulation and Amplitude Modulation function and obtained, and will be modulated piece placement and is filtered the focal beam spot that shaping obtains Fast Convergent to light beam in the optical path.The utility model changes the convergence of focal beam spot using Modulation and Amplitude Modulation piece with less cost and technical difficulty, reduces the other spot intensity of diffraction pattern, in bio-imaging, the numerous areas such as laser optics has huge application value and potentiality.
Description
Technical field
The utility model is related to laser beam shaping field, more particularly to a kind of light beam of focal beam spot Fast Convergent is whole
Shape modulates piece, and the Fast Convergent of focal beam spot is realized using the Modulation and Amplitude Modulation of light beam.
Background technology
From nineteen sixty First laser come out since, laser by its good monochromaticjty, directionality, coherence and
Energy density is big to wait excellent characteristics, is gradually obtained extensively in various fields such as physics, chemistry, biology, material, medical science, information technologies
General application.Hereafter, laser technology is constantly permeated to other field, has greatly promoted basis and the application of other related disciplines
Key player is play in research, such as photoelectric technology, biologic medical, material processing, accurate measurement various fields.With sharp
The development of light technology, beam shaping technology turns into a kind of hot technology, and the light beam of special shape can be applied to laser optics and life
The fields such as thing medical science.For example, flat top beam can be applied to the fields such as laser fusion, laser heat treatment, bottle beams exist
All it is widely used in terms of atom guiding, Trapping of Atoms, and optical tweezers.Therefore, the polarization state of light beam, phase are passed through
With the space structure design of amplitude, realizing the Effective Regulation of three-dimensional focal light field has important application value.
The novel focus characteristic and the new focusing shaping technique of exploration of different light beams always are that the main of optical field is ground
Study carefully one of problem.When incident light source is focused on by high numerical aperture lens, because the vector property of light beam changes, in the past
The scalar diffraction theory that light beam Study on focusing characteristic is used no longer is applicable, and uses Richards-Wolf Vector Diffraction Theories
Study the tightly focused characteristic of light beam.Under the conditions of tightly focused, radial polarisation light can form very strong longitudinal light field in focus;And
Angularly polarized light can then form purely lateral hollow ring optical field distribution;Broad sense axially symmetry polarization light shape after lens tightly focused
Into a flat-head type optical field distribution;Circular polarization vortex beams can realize spin angular momentaum and track angular motion under the conditions of tightly focused
Part conversion between amount.The research of light beam tightly focused characteristic has attracted the concern of a large amount of researchers.
To design three-dimensional focal light field special and with practical value, people have successively attempted many focusing shapings
Method.Binary diffractive optical element (DOE) is added such as before lens to adjust the polarization of incident beam, phase and amplitude
System.2002, Zhan et al. was modulated by using polarization apparatus for turning modulation to the polarization direction of column symmetry vector light,
The focusing light field of " spike ", " annulus " and " flat-head type " distribution is obtained respectively.Wang in 2008 et al. utilizes radial polarisation shellfish plug
That-Gaussian beam combines five annular DOE phase-modulators, and a depth of focus is being generated after high numerical aperture lens tightly focused
For " laser accunputure " of the pure longitudinal field of 4 wavelength.Hereafter, Lin et al. generates one by using the method for Modulation and Amplitude Modulation and is up to 9
The laser accunputure of individual wavelength.However, so far, still without a kind of light-beam forming unit for focusing on light field Fast Convergent.
Utility model content
To realize the Fast Convergent of Laser Focusing hot spot, the utility model provides a kind of light of focal beam spot Fast Convergent
Piece is modulated in beam shaping.The amplitude of incident light is modulated using Modulation and Amplitude Modulation piece, the hot spot of focusing light field is presented different
Convergence property, reduces the other spot intensity of diffraction fringe, optimizes the rate of decay of light intensity.In fields such as bio-imaging, life sciences
There is important application value.
The utility model use concrete technical scheme be:
The beam shaping modulation piece is optical filter, and its light intensity transmitance distribution center is symmetrical, the everywhere light of its radial direction
Strong transmitance uses Modulation and Amplitude Modulation function to calculate and obtained, and the distribution of light intensity transmitance is to be justified by Modulation and Amplitude Modulation function around through modulation piece
The heart and turn to obtain along the axle rotation optical axis of piece (beam shaping modulate) of plane normal direction where modulation piece.
Described Modulation and Amplitude Modulation function is represented using below equation, as shown in figure 1, being isopleth between the curve of both sides, is shaken
Width modulation function calculates the value P obtainedθ(t) it is the setting value of light intensity transmitance:
Wherein, t represents energy coefficient, t=ρ2, ρ represent modulate the polar radius of piece, θ represent modulation piece it is polar
Angle, x represents the abscissa (i.e. along the coordinate of modulation piece radial direction) of Modulation and Amplitude Modulation function, τLRepresent to restrain on the inside of modulation piece
Coefficient, τRConvergence coefficient on the outside of modulation piece is represented, f (x, τ) represents convergent function, and τ represents convergence coefficient, and convergence coefficient τ is represented
τLOr τR, e represents constant e.
Beam shaping modulation piece of the present utility model is modulated to the amplitude of incident light, changes focus point interference optical field
Light distribution, changes the hot spot convergence for focusing on light field, reduces the other spot intensity of diffraction fringe, and the decay for optimizing light intensity is special
Property, realize the Fast Convergent of focal beam spot.
The light intensity transmitance convergence of the beam shaping modulation piece internal circumference and exterior circumference is identical or differed.
The utility model is placed using described modulation piece is filtered shaping acquisition Fast Convergent to light beam in the optical path
Focal beam spot.Spot decay is most strong by the Modulation and Amplitude Modulation function of proposition, and its light intensity transmitance is distributed as shown in Fig. 2 being adjusted by amplitude
Function generation processed, white represents transmitance highest, and whiter expression transmitance is higher, and black represents that transmitance is minimum, more black to represent
Transmitance is lower.Modulation and Amplitude Modulation is carried out to light beam using Modulation and Amplitude Modulation piece of the present utility model, the convergence for changing focal beam spot is special
Property.Hot spot Fast Convergent produced by the light beam modulated by the light intensity transmittance function, the other spot for focusing on light field is greatly reduced.
The beam shaping modulation piece is applied in Modulation and Amplitude Modulation Laser Scanning Confocal Microscope.
Modulation and Amplitude Modulation Laser Scanning Confocal Microscope mainly includes confocal microscope system and Modulation and Amplitude Modulation piece two parts, and amplitude is adjusted
Film-making is placed in before the object lens of Laser Scanning Confocal Microscope, and Modulation and Amplitude Modulation is carried out to exciting light, and the original structure of Laser Scanning Confocal Microscope can be protected
Hold constant, the signal to noise ratio of Laser Scanning Confocal Microscope Depth Imaging is significantly improved with relatively low cost and technical difficulty.
The beneficial effects of the utility model are:
The utility model is used to carry out Modulation and Amplitude Modulation to incident light, changes the interference optical field distribution of hot spot focusing surface, makes to gather
Different convergence properties are presented in the hot spot of burnt light field, reduce the other spot intensity of diffraction fringe, optimize the rate of decay of light intensity.
The technical solution of the utility model cost is very low, but highly effective, practical, with very strong application value.
Brief description of the drawings
Fig. 1 is the curve of Modulation and Amplitude Modulation function;
Fig. 2 is Modulation and Amplitude Modulation optical filter;
Fig. 3 is that the Modulation and Amplitude Modulation of embodiment 1 focuses on the amplitude point spread function comparison diagram with commonly focusing on;
Fig. 4 is that the Modulation and Amplitude Modulation of embodiment 1 focuses on the attenuation characteristic comparison diagram with commonly focusing on;
Fig. 5 is that the Modulation and Amplitude Modulation of embodiment 1 focuses on beam speckle contrast's figure with commonly focusing on;
Fig. 6 is the Modulation and Amplitude Modulation schematic diagram of the confocal microscopic image of embodiment 2;
Fig. 7 is the axial intensity distribution of the Modulation and Amplitude Modulation confocal microscope system of embodiment 2;
Fig. 8 is that the Modulation and Amplitude Modulation confocal microscope system of embodiment 2 is used for the hot spot and attenuation function of Depth Imaging;
Fig. 9 is that the confocal microscope system of embodiment 2 is used for hot spot, attenuation function and the axial intensity point of Depth Imaging
Cloth;
Figure 10 Modulation and Amplitude Modulation focusing principle figures;
Figure 11 is the curve of Modulation and Amplitude Modulation function;
Figure 12 is Modulation and Amplitude Modulation optical filter;
Figure 13 is the point spread function of the Modulation and Amplitude Modulation focusing of embodiment 3;
Figure 14 is beam speckle contrast's figure of the Modulation and Amplitude Modulation focusing of embodiment 3.
In figure:Laser 1, the first aperture diaphragm 2, the first object lens 3, dichroic mirror 4, modulation piece 5, the second object lens 6, sample 7,
Focusing surface 8, scan table 9, the 3rd object lens 10, the second aperture diaphragm 11, detector 12.
Embodiment
Describe the utility model in detail with reference to embodiment and accompanying drawing, but the utility model is not limited to that.
Embodiment and its implementation process of the present utility model are as follows:
Embodiment 1
The present embodiment is applied to single laser, object lens and detector and constituted in light path, and modulation piece is placed in laser and thing
Between mirror.
The present embodiment uses one of canonical parameter, τL=τR=0.25, now, Modulation and Amplitude Modulation function is FAM.
Modulation and Amplitude Modulation function along the rotation of x=0 axles by Fig. 1 as shown in figure 1, can obtain Modulation and Amplitude Modulation optical filter (i.e. this reality
Use new type of modulation piece) transmitance distribution, the light intensity transmitance of Modulation and Amplitude Modulation optical filter is as shown in Figure 2.
Point spread function and without Modulation and Amplitude Modulation point spread function contrast as shown in figure 3, wherein dotted line for pass through
The situation of Modulation and Amplitude Modulation, and solid line is the situation without Modulation and Amplitude Modulation.
The point spread function when rate of decay of focal beam spot by Modulation and Amplitude Modulation is significantly faster than that no amplitude polarizer is such as
Shown in Fig. 4, wherein dotted line is the situation Jing Guo Modulation and Amplitude Modulation, and solid line is the situation without Modulation and Amplitude Modulation.
The hot spot of generation is as shown in figure 5, left figure is without Modulation and Amplitude Modulation, and right figure is to pass through the utility model Modulation and Amplitude Modulation
Hot spot afterwards, it is seen that now spot almost disappears by hot spot.
Embodiment 2
The present embodiment is applied in Modulation and Amplitude Modulation Laser Scanning Confocal Microscope, and Modulation and Amplitude Modulation Laser Scanning Confocal Microscope includes common be total to
Focus on microscopic system and the utility model modulation piece.Modulation and Amplitude Modulation Laser Scanning Confocal Microscope is aobvious by Modulation and Amplitude Modulation piece and common copolymerization Jiao
Micro-system is constituted, and Modulation and Amplitude Modulation piece is placed in before the focusing objective len of Laser Scanning Confocal Microscope, and the exciting light for inciding sample is carried out
Modulation and Amplitude Modulation.
It is specific as shown in fig. 6, common confocal microscope system include laser 1, the first aperture diaphragm 2, the first object lens 3,
Beam splitter 4, the second object lens 6, sample 7, focusing surface 8, scan table 9, the 3rd object lens 10, the second aperture diaphragm 11 and detector 12,
The laser that laser 1 is sent incides beam splitter 4 after the first aperture diaphragm 2, the first object lens 3 successively, and through beam splitter, 4 points are two
Shu Guang, light beam is received after the 3rd object lens 10, the second aperture diaphragm 11 by detector 12 successively, and another light beam is modulated successively
It is irradiated on sample 7 and is focused in focusing surface 8 after piece 5, the second object lens 6, sample 7 is placed on scan table 9.
Specific implementation is loaded on modulation piece 5 using the Modulation and Amplitude Modulation function FAM in embodiment 1 and tested, Ran Hou
During depth micro-imaging, rapid decay characteristic is presented in focal beam spot, and other spot greatly reduces, reduces noise intensity, improve letter
Make an uproar ratio.
As shown in fig. 7, the excitating light strength of sample axial direction (longitudinal direction) can be by the spot intensity distribution (left figure) in Fig. 8 and light
Strong attenuation rate (right figure), which is multiplied, to be obtained.Noise is mainly caused by sample surfaces to the excitation focus point, by Fig. 7 with
It can see without the contrast of hot spot distribution map 9 after Modulation and Amplitude Modulation, when imaging depth is 0.1mm, Modulation and Amplitude Modulation copolymerization Jiao is micro-
Significantly lower than common Laser Scanning Confocal Microscope, (signal to noise ratio improves 6.75 times to the noise intensity of mirror, and as depth increases, signal to noise ratio is again
Number is bigger).By simplation verification, find in the case of the parameter, when imaging depth is more than 66um, Modulation and Amplitude Modulation copolymerization Jiao is aobvious
The signal to noise ratio of micro mirror is better than common Laser Scanning Confocal Microscope, and in actual applications, in the case of high NA, the depth threshold can be more
It is small.
Laser Scanning Confocal Microscope uses conjugate focus technology, and illumination pin hole, detector pin hole and sample are conjugated each other.Exciting light
Beam focuses on sample plane by object lens, and the be stimulated fluorescence of injection of sample is focused on detector pin hole along imaging optical path, is passed through
The space filtering effect of needle passing hole, inhibits the veiling glare outside focal plane to greatest extent so that the fluorescence letter that only focus is sent
It number could be received by photodetector, significantly improve the signal to noise ratio and resolution ratio of system.Laser Scanning Confocal Microscope passes through to sample
Product confocal plane carries out the optical chromatography of point by point scanning and Z-direction, realizes the three-dimensional imaging to thicker sample.
Embodiment 3
The present embodiment is applied to be made up of in light path laser, object lens and detector, as shown in Figure 10.
The present embodiment is using a random parameter, τL=0.2, τR=0.15, now, Modulation and Amplitude Modulation function is:
Figure 11 as shown in figure 11, can obtain Modulation and Amplitude Modulation optical filter by corresponding Modulation and Amplitude Modulation function along the rotation of x=0 axles
The transmitance distribution of (i.e. the utility model modulation piece), the light intensity transmitance of Modulation and Amplitude Modulation optical filter is as shown in figure 12.
As shown in figure 13, the hot spot of generation is as shown in figure 14 for point spread function during the parameter, it is seen that now spot by hot spot
Less than the situation without Modulation and Amplitude Modulation, more than the situation of the canonical parameter in embodiment 1, but center spot is less than embodiment 1
In situation.
Simple and easy to apply, cost of the invention is relatively low it can be seen from three above embodiment, can greatly improve depth micro-
The signal to noise ratio of imaging, there is higher practical value in fields such as bio-imagings, can also be adjusted according to change amplitude the need for difference
Function processed.
Claims (3)
1. a kind of beam shaping modulation piece of focal beam spot Fast Convergent, it is characterised in that:The beam shaping modulation piece is filter
Mating plate, its light intensity transmitance distribution center is symmetrical, and the everywhere light intensity transmitance of its radial direction is calculated using Modulation and Amplitude Modulation function to be obtained
, Modulation and Amplitude Modulation function is represented using below equation, and Modulation and Amplitude Modulation function calculates the value P obtainedθ(t) setting for light intensity transmitance
Definite value:
Wherein, t represents energy coefficient, t=ρ2, ρ represent modulate piece radius, θ represent modulate piece angle, x represent along modulation piece
The coordinate of radial direction, τLRepresent convergence coefficient, τ on the inside of modulation pieceRConvergence coefficient on the outside of modulation piece is represented, f (x, τ) represents to receive
Function is held back, τ represents convergence coefficient, and convergence coefficient τ represents τLOr τR, e represents constant e.
2. a kind of beam shaping modulation piece of focal beam spot Fast Convergent according to claim 1, it is characterised in that:It is described
Beam shaping, which modulates piece internal circumference and the light intensity transmitance convergence of exterior circumference, to be identical or differs.
3. a kind of beam shaping modulation piece of focal beam spot Fast Convergent according to claim 1, it is characterised in that:It is described
Modulation piece is placed and is filtered the focal beam spot that shaping obtains Fast Convergent to light beam in the optical path.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106855657A (en) * | 2017-01-11 | 2017-06-16 | 浙江大学 | Beam shaping modulation piece and its method for focal beam spot Fast Convergent |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106855657A (en) * | 2017-01-11 | 2017-06-16 | 浙江大学 | Beam shaping modulation piece and its method for focal beam spot Fast Convergent |
CN106855657B (en) * | 2017-01-11 | 2023-05-26 | 浙江大学 | Beam shaping modulation sheet capable of quickly converging focusing light spot and method thereof |
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