CN206369864U - A kind of small-scale structure light microscope system - Google Patents
A kind of small-scale structure light microscope system Download PDFInfo
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- CN206369864U CN206369864U CN201621411672.2U CN201621411672U CN206369864U CN 206369864 U CN206369864 U CN 206369864U CN 201621411672 U CN201621411672 U CN 201621411672U CN 206369864 U CN206369864 U CN 206369864U
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Abstract
The utility model discloses a kind of small-scale structure light microscope system, and it includes:LASER Light Source, collimator objective, extender lens group, 1/4 λ pieces, PBS, LCOS, pipe lens, object lens and objective table, are provided with MASK on the entrance pupil face of object lens, and MASK is used for the light for filtering different levels.It is complicated that a kind of small-scale structure light microscope of the utility model solves existing system, volume is big, the effect of imaging is not particularly preferred problem, the utility model system architecture is simple, using laser as light source, on the basis of the resolution ratio of system is reasonably retained, the illuminator and imaging system traditional by separating, it is greatly improved the expansion of the system, and the combination of conventional microscope just can realize the function of super-resolution microscopic system, realize and greatly reduce volume on the basis of certain systemic resolution is kept.
Description
Technical field
The utility model is related to a kind of small-scale structure light microscope system.
Background technology
Traditional optical microscope system is limited by optical diffraction limit, and it is generally advisable to can only achieve for horizontal resolution ratio
200nm, longitudinal resolution ratio reaches 500nm, it is impossible to meet present field of biology for micro-structures such as subcellular structures
The demand of research.
Structure light micro-imaging technique is initially proposed that resolution ratio can be improved compared to traditional microscope by Neil et al.
1 times (i.e. 100nm).The main Structured Illumination sample using particular modulation, with the algorithm of the image procossing in later stage, from difference
The information of sample focal plane is extracted in the modulation image of phase, so as to obtain the view data of structure light micro-imaging, is realized super
Resolution ratio.
Traditional structure light microscopic system is broadly divided into two big types according to the difference of light source:Using LED as the structure of light source
Light microscopic system;Structure light microscopic system by light source of laser.
The current structure light microscopic system by light source of LED, system architecture is relatively easy (see Fig. 1), but wants at present
Relatively good image is obtained, it is necessary to the light source of better performances, and compared with the volume of long light-path, so as to limit its application.
And the structure light microscopic system by light source of laser, though preferable image can be obtained, generally there is complicated system architecture
(see Fig. 2), it is costly.
The structure light microscope system that current tradition is used uses optical grating construction, main optical routing lighting source, lens
System, grating, detector and synchronous control system composition.The light that light source is sent passes through the collimation of lens, and institute is produced through grating
Object under test surface is arrived in the structure light needed, illumination, and the fluorescence signal that body surface is sent is imaged in into detection by lens combination
On the target surface of device.The speed for making grating movement be recorded with detector using piezo-electric device is synchronized, and has gathered piece image letter
After breath, grating moves a segment distance, records the position of new images.Figure information is handled using algorithm afterwards, excess of export is reconstructed
High-resolution two dimensional image.The characteristics of this kind of system is that light channel structure is simple, image taking speed is very fast.But, due to needing to move
Dynamic grating, this may result in time precision in the precision that displacement is produced in moving process, synchronizing process, signal to noise ratio is low asks
Topic.
Utility model patent 200810071654.8 proposes a kind of layer based on two-dimensional modulation technology and cuts image acquisition side
Method, this method utilizes two dimensional spatial light modulator such as DMD (digital micro-mirror), penetration liquid crystal LCD, reflective type silicon-based liquid crystal
LCOS etc. replaces the grating in traditional structure light microscope, two-dimensional modulation is carried out to illumination, and produce the tune of one group of out of phase
Pattern, rebuilds with reference to two-dimentional phase shift algorithm and cuts tomographic image.
Utility model patent 201110448980.8 proposes a kind of high-speed structures illumination based on digital micro-mirror device
Optical microscope system and method, including light source, be arranged on lighting source road structure photogenerator, be arranged on Amici prism
Lens on projecting light path, the spectroscope being arranged in lens light path, it is arranged on reflective mirror and cylinder in light path below spectroscope
Mirror, it is arranged on a CCD camera at mirror rear.
Above two utility model patent represents the micro- technology of current most of Structured Illuminations, its main shortcoming
It is complicated, volume is big, image taking speed appoints the shortcomings of being so restricted.
Utility model patent 201410036645.O discloses a kind of Structured Illumination optical system, including:It is computer, same
Walk control system, ultra-high brightness LED excitation source, relay lens group, fly eye lens array, intermediate image plane, dichroscope, projection
Object lens, objective table, cylinder mirror, narrow band pass filter and photodetector.The effect that above-mentioned patent has fly's-eye lens is bad, can make
The stripe effect of generation declines, and reduces the resolution ratio of image, and above-mentioned structure is currently without the progress of related experiment image
Checking.
Utility model content
The utility model in view of the above-mentioned problems, propose a kind of small-scale structure light microscope system, its is simple in construction, with
Laser is light source, on the basis of the resolution ratio of system is reasonably retained, by structure optimization, greatly reduces the body of system
Product, reduces cost, greatly improves systematic difference scope.
In order to achieve the above object, the technical solution of the utility model is as follows:
A kind of small-scale structure light microscope system, including:LASER Light Source, collimator objective, extender lens group, 1/4 λ pieces,
PBS, LCOS, pipe lens, object lens and objective table, are provided with MASK on the entrance pupil face of object lens, and MASK is used to filter different levels
Light;
LASER Light Source carries out the adjustment in polarization of light direction by 1/4 λ pieces after being collimated by collimator objective, then by expanding
After lens group is expanded, light is squeezed on LCOS by PBS, is realized matched with object lens entrance pupil face footpaths by pipe lens afterwards,
Laser is driven on the MASK in object lens entrance pupil face, by object lens by Structured Illumination on the sample surface of objective table, realize structure
The illumination of light.
A kind of small-scale structure light microscope system of the utility model loads MASK on the entrance pupil face of object lens, can be very big
Shortening light path, reduce volume, the illuminator and imaging system traditional by separating are greatly improved opening up for the system
Exhibition, and the combination of conventional microscope just can realize the function of super-resolution microscopic system, realize and keeping certain system
Greatly reduce volume on the basis of resolution ratio.
On the basis of above-mentioned technical proposal, following improvement can be also done:
As preferred scheme, LASER Light Source is sent by laser.
Using above-mentioned preferred scheme, stable laser beam can be launched.
As preferred scheme, laser carries out light by optical fiber to be directed into collimator objective.
Using above-mentioned preferred scheme, imported light into using the mode of laser coupled into optical fibres.
As preferred scheme, provided with multiple hole positions for being used to filter different level light on MASK.
Using above-mentioned preferred scheme, MASK can allow required structure light to pass through.
As preferred scheme, multiple hole positions are uniformly distributed using the center in object lens entrance pupil face as the center of circle along its circumferencial direction.
Using above-mentioned preferred scheme, uniform illumination.
As preferred scheme, the number of hole position is even number.
Using above-mentioned preferred scheme, even number hole position can be effectively symmetrical.
As preferred scheme, extender lens group includes the first lens and the second lens be arrangeding in parallel.
It is simple in construction using above-mentioned preferred scheme, light beam is expanded, the miniaturization of structure is effectively realized.
As preferred scheme, collimator objective, extender lens group, 1/4 λ pieces, PBS, LCOS, pipe lens and object lens are set
In in housing.
Using above-mentioned preferred scheme, device overall simple, and housing can be fixed and protect to internal part.
Brief description of the drawings
Fig. 1 is the structural representation of LED structure light microscope system in the prior art.
Fig. 2 is the structural representation of laser structure light microscope system in the prior art.
The optical texture schematic diagram for the small-scale structure light microscope system that Fig. 3 provides for the utility model embodiment.
One of structural representation of object lens equipped with MASK that Fig. 4 provides for the utility model embodiment.
The two of the structural representation for the object lens equipped with MASK that Fig. 5 provides for the utility model embodiment.
The external physical structural representation for the small-scale structure light microscope system that Fig. 6 provides for the utility model embodiment
Figure.
The internal physical structure signal for the small-scale structure light microscope system that Fig. 7 provides for the utility model embodiment
Figure.
Wherein:11-LED chips;12- low power objectives or the good lens of achromatism;13- lens;14- lens;15-DMD
(digital micro-mirror);16- pipe lens;17- object lens;18- objective tables;
21- optical fiber;22- low power objectives or the good lens of achromatism;23- lens;24- lens;(liquid crystal is empty by 25-LCOS
Between optical modulator);26-PBS;27- lens;28-MASK;29- lens;210- speculums;211-LCC (liquid crystal delayer);
212- pipe lens;213- object lens;14- objective tables;
31- lasers;32- optical fiber;33- collimator objectives;34-1/4 λ pieces;356- extender lens groups;The lens of 35- first;
The lens of 36- second;37-LCOS;38-PBS;39- pipe lens;310MASK;311- object lens;312- objective tables;313- housings.
Embodiment
Describe preferred embodiment of the present utility model in detail below in conjunction with the accompanying drawings.
In order to reach the purpose of this utility model, a kind of some of embodiments of small-scale structure light microscope system
In,
As shown in Figures 3 and 4, a kind of small-scale structure light microscope system includes:Single-mode fiber 32 is passed through by laser 31
LASER Light Source, collimator objective 33,1/4 λ pieces 34, extender lens group 356, LCOS37, PBS38, pipe lens 39, the object lens of importing
311 and objective table 312, MASK310 is provided with the entrance pupil face of object lens 311, MASK310 is used for the light for filtering different levels.
Extender lens group includes the first lens 35 and the second lens 36 be arrangeding in parallel.
In the present embodiment, collimator objective 33 selects the good low power number object lens of low power number object lens achromatism effect.
MASK310 can be directly glued on the entrance pupil face of object lens 311, can also be added in the manufacturing process of object lens 311
MASK310。
It is used to provided with six filter the hole positions of different level light on MASK310, so that MASK310 can allow required knot
Structure light passes through, and six hole positions are uniformly distributed using the center in the entrance pupil face of object lens 311 as the center of circle along its circumferencial direction.
Except above-mentioned optical system, in specific implementation process, its also need to imaging system, synchronous control system with
And follow-up image processing algorithm, current the two parts of most system are using conventional CCD collection images, with increasing income
Structure light processing algorithm software processing image, produce high-definition picture.
A kind of light path of small-scale structure light microscope system of the utility model is as follows:
Laser 31 by optical fiber 32 import LASER Light Source, LASER Light Source by collimator objective 33 collimate after by 1/4 λ pieces
34 carry out the adjustment in polarization of light direction, then after extender lens group is expanded, are squeezed into light on LCOS37 by PBS38, it
Realized and matched with the entrance pupil face footpath of object lens 311 by pipe lens 39 afterwards, laser is driven into the MASK310 in the entrance pupil face of object lens 311
On, by object lens 311 by Structured Illumination on the sample surface of objective table 312, realize the illumination of structure light.
A kind of small-scale structure light microscope system of the utility model loads MASK310 on the entrance pupil face of object lens 311, can
Greatly to shorten light path, reduce volume, by the change of optical system and corresponding construction, realize and keeping certain system
Greatly reduce volume on the basis of resolution ratio, expand the scope of its application.
The utility model illuminator and imaging system traditional by separating, are greatly improved the expansion of the system,
And the combination of conventional microscope just can realize the function of super-resolution microscopic system.Used compared to conventional super-resolution system
Illumination and imaging share an object lens, and it is inconvenient to expand, and the application can be separated effectively, and illuminator is with imaging
System is each to use corresponding object lens, is more convenient for expanding.
The utility model is imported light into using the mode of the coupled into optical fibres 32 of laser 31, can also use optical fiber laser
Or the introducing of LASER Light Source is directly carried out, by the good low power number object lens of achromatism effect, light is collimated, passes through 1/
4, which enter piece 34, carries out the adjustment in light polarization direction, and is expanded by one group of extender lens group, with 39 groups of pipe lens below
Conjunction realization is matched with the entrance pupil face footpath of object lens 311, realizes best optical characteristics.Afterwards, light is squeezed into by PBS38
On LCOS37, it is driven into afterwards by pipe lens 39 on MASK310, MASK310 loads on the entrance pupil face of object lens 311, Ke Yiji
Big shortening light path, reduces volume, the level of the structure light of needs can be branched away, by object lens 311 by Structured Illumination
On the sample surface of objective table, the illumination of structure light is effectively realized.It is worth noting that, MASK310 effectively arrives the different levels of filter
Light effect, allow required structure light to pass through, it needs high machining accuracy and its symmetry.
The utility model devises a kind of small-scale structure light microscope system, has passed through simple structure and optical system
Design, realize the miniaturization of Structured Illumination system, a kind of realization easily and fast provided for super-resolution micro-imaging
System, provides a kind of easily imaging system for research biological sample, can obtain relatively good image, solve existing
System architecture is complicated, and volume is big, the problem of effect of imaging is not particularly good.
As shown in figure 5, in order to further optimize implementation result of the present utility model, in other embodiment,
Remaining feature technology is identical, and difference is, provided with 12 hole positions for being used to filter different level light on MASK310.
The hole position of six numbers different with 12 is set, is mainly used in realizing linear structure optical illumination and nonlinear organization
The difference of optical illumination, improves resolution ratio.
As shown in Figures 6 and 7, in order to further optimize implementation result of the present utility model, in other embodiment
In, remaining feature technology is identical, and difference is, collimator objective 33, extender lens group, 1/4 λ pieces 34, PBS38, LCOS37,
Pipe lens 39 and object lens 311 are arranged in housing 313.Its device overall simple, and housing 313 can enter to internal part
Row is fixed and protects, due to the utility model proposes small-scale structure light microscope system miniaturization, the chi of its housing 313
The wide * height of modest ability * is no more than 250*250*150mm.
The above is only preferred embodiment of the present utility model, it is noted that for one of ordinary skill in the art
For, on the premise of the utility model creation design is not departed from, various modifications and improvements can be made, these belong to this
The protection domain of utility model.
Claims (8)
1. a kind of small-scale structure light microscope system, including:LASER Light Source, collimator objective, extender lens group, 1/4 λ pieces,
PBS, LCOS, pipe lens, object lens and objective table, it is characterised in that MASK is provided with the entrance pupil face of the object lens, it is described
MASK is used for the light for filtering different levels;
The LASER Light Source carries out the adjustment in polarization of light direction by the 1/4 λ pieces after being collimated by the collimator objective, then
After the extender lens group is expanded, light is squeezed on the LCOS by the PBS, realized afterwards by the pipe lens
With matching for the object lens entrance pupil face footpath, laser is driven on the MASK in the object lens entrance pupil face, passes through the object lens
By Structured Illumination on the sample surface of the objective table, the illumination of structure light is realized.
2. small-scale structure light microscope system according to claim 1, it is characterised in that the LASER Light Source is by laser
Device is sent.
3. small-scale structure light microscope system according to claim 2, it is characterised in that the laser passes through optical fiber
Light is carried out to be directed into the collimator objective.
4. the small-scale structure light microscope system according to claim any one of 1-3, it is characterised in that in the MASK
Provided with multiple hole positions for being used to filter different level light.
5. small-scale structure light microscope system according to claim 4, it is characterised in that multiple hole positions are with described
The center in object lens entrance pupil face is that the center of circle is uniformly distributed along its circumferencial direction.
6. small-scale structure light microscope system according to claim 5, it is characterised in that the number of the hole position is even
It is several.
7. small-scale structure light microscope system according to claim 6, it is characterised in that the extender lens group includes
The first lens and the second lens be arrangeding in parallel.
8. small-scale structure light microscope system according to claim 7, it is characterised in that the collimator objective, described
Extender lens group, the 1/4 λ pieces, the PBS, the LCOS, the pipe lens and the object lens are arranged in housing.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106707485A (en) * | 2016-12-21 | 2017-05-24 | 中国科学院苏州生物医学工程技术研究所 | Miniature structure light microscopic illumination system |
CN108897126A (en) * | 2018-08-09 | 2018-11-27 | 中国科学院半导体研究所 | A kind of fluoroscopic imaging systems |
-
2016
- 2016-12-21 CN CN201621411672.2U patent/CN206369864U/en not_active Withdrawn - After Issue
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106707485A (en) * | 2016-12-21 | 2017-05-24 | 中国科学院苏州生物医学工程技术研究所 | Miniature structure light microscopic illumination system |
CN106707485B (en) * | 2016-12-21 | 2024-05-03 | 中国科学院苏州生物医学工程技术研究所 | Small-size structure light microscopic lighting system |
CN108897126A (en) * | 2018-08-09 | 2018-11-27 | 中国科学院半导体研究所 | A kind of fluoroscopic imaging systems |
CN108897126B (en) * | 2018-08-09 | 2020-11-03 | 中国科学院半导体研究所 | Fluorescence imaging system |
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