CN206369700U - A kind of quick residues of pesticides test card efficiently with low cost - Google Patents

A kind of quick residues of pesticides test card efficiently with low cost Download PDF

Info

Publication number
CN206369700U
CN206369700U CN201720030881.0U CN201720030881U CN206369700U CN 206369700 U CN206369700 U CN 206369700U CN 201720030881 U CN201720030881 U CN 201720030881U CN 206369700 U CN206369700 U CN 206369700U
Authority
CN
China
Prior art keywords
test card
residues
quick
frid
observation window
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720030881.0U
Other languages
Chinese (zh)
Inventor
陈静
王超
沈丽
蒋雅慧
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Wuzi University
Original Assignee
Beijing Wuzi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Wuzi University filed Critical Beijing Wuzi University
Priority to CN201720030881.0U priority Critical patent/CN206369700U/en
Application granted granted Critical
Publication of CN206369700U publication Critical patent/CN206369700U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The utility model discloses a kind of quick residues of pesticides test card efficiently with low cost, it is related to residues of pesticides technical field of measurement and test;The right-hand member of the handle is provided with package shelf, centre inside the package shelf is provided with splash guard, the upper and lower sides of the splash guard are provided with test card body, instillation frid is provided with upper and lower end face on front side of the package shelf, observation window is provided with the left of the instillation frid, the inside of the observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla;The utility model is easy to implement quick detection, and easy to operate, and the degree of accuracy is high, saves the time.

Description

A kind of quick residues of pesticides test card efficiently with low cost
Technical field:
The utility model is related to a kind of quick residues of pesticides test card efficiently with low cost, belongs to residues of pesticides test skill Art field.
Background technology:
The method of existing detection organophosphorus pesticide residual quantity is a lot, mainly there is chromatography, immunization, chemiluminescence at present Method and inhibiting AChE.
1st, chromatography is most classical, the most frequently used technology in residues of pesticides conventional detection.General use gas-chromatography (GC), Liquid chromatography (LC), high performance liquid chromatography (HPLC), compounds GC-MS (GC-MS), supercritical fluid chromatography technology (SFC), exempt from The technologies such as epidemic disease affinity chromatography technology (IAC), gel permeation chromatography technology (GPC) will need to enter by the sample strictly purified after extraction Row detection.This method great advantage is that sensitivity is high, the degree of accuracy is good, and qualitative, the quantitative result of examined agricultural chemicals can be provided simultaneously, is carried For arbitration foundation.But there is also to technical requirements are big compared with high, equipment investment, sample preprocessing is cumbersome, censored time length, spend The shortcomings of cost is high.As (GC-MS) can measure the range of linearity, regression equation, coefficient correlation, the rate of recovery and the correlation of 32 kinds of agricultural chemicals Standard deviation.
Gas chromatography is the topmost assay method of current environment sample Pesticide Residues, can be to most of residues of pesticides Accurate Analysis is measured, measurement is accurate, is national standard pesticide residue analysis method, minimum detection output is up to ng.But selectivity and Sensitivity is not high, and pretreatment is cumbersome, using narrow range, is only applicable to volatility agricultural chemicals.
2nd, immunoassay is a kind of using antibody as biosensors, and the materials such as compound, enzyme or protein are carried out The analytical technology of qualitative and quantitative analysis.It is in detection grain, water fruits and vegetables, meat, milk, water and soil since 1990s Developed rapidly in terms of the residual of earth Pesticides.Technique, the U.S. are recommended to many countries by the World Food Programme (FAO) Immuno analytical method, chromatographic technique are classified as the major technique of pesticide residue analysis by Chemical Society jointly, its knot detected Fruit has legal effect.It is to be combined the Detecting Pesticide side based on reflecting with specificity, the invertibity of antibody by antigen Method, the mode of predominantly detecting is to use kit.This method has that selectivity is strong, sensitivity is high, quick, simple operation and other advantages.But Due to, immunogene preparation complexity various by pesticide variety, immune response is with the influence in terms of specificity, its application It is subject to certain restrictions.
3rd, inhibiting AChE is to suppress some specific biology enzymes (such as acetylcholinesterase, carboxy-lesterase) based on agricultural chemicals Activity, and by specific chromogenic reaction, observe by the naked eye or instrument readings judged, qualitatively or quantitatively to detect sample Product Pesticide Residues situation, the existing considerably long history of application of this method, and still have suitable use value so far, can Residual hazard for Multiple Pesticides is determined.Target organismses enzyme can be placed in chromatographic sheet, the scraps of paper or test tube using this principle In, enzyme is reacted with sample, residues of pesticides or residual quantity are extremely low if do not had in sample, and enzymatic activity is just not suppressed, bottom Thing can be hydrolyzed, and hydrolysate is developed the color by with specific developer reaction solution or having color in itself;, whereas if examination Have the remains of pesticide of higher amount in sample, enzymatic activity is suppressed by agricultural chemicals, substrate can not be hydrolyzed, so as to can not develop the color, thin-layer method and The result judgement of paper disk method can by develop the color degree size or spot size, weigh, leaching etc. carries out qualitative or sxemiquantitative point Analysis, colorimetric method can calculate the inhibition level of enzymatic activity using absorbance, so as to judge whether there is residual toxicity in sample.
The reaction substrate commonly used in inhibiting AChE has acetyl oxyindole, the bromo- oxyindoles of acetic acid -5-, acetic acid-α-naphthylacetate Or carboxylate, chlorine (bromine, iodine) change acetylcholine, indophenols acetic acid esters etc..Conventional coloration method has:
1. it is acetyl and choline using acetylcholine hydrolyzation product, is made by acid-base indicator bromthymol blue and acetic acid With, aobvious blueness when residues of pesticides are high, displaing yellow is distinguished when non agricultural chemical residuum or low persticide residue;
2. act on forming aubergine azo using substrate β naphthyl acetate hydrolysate betanaphthol and developer Fast blue B Compound;
3. acetic acid oxyindole hydrolysate indoxyl autoxidation is utilized into indigo and aobvious blueness;
4. the color of indophenols Acetate hydrolysis product itself is utilized;
5. acetylthiocholine is changed using substrate iodine (bromine, chlorine), under AchE catalysis, the iodine (bromine, chlorine) for decomposing generation is changed Thiocholine, with 5,5 '-two thio -2,2 ' dinitrobenzoic acids (DTNB) effect, generation 5- coloured glaze base -2- nitrobenzoic acid (Huangs Color).
The major advantage of inhibiting AChE detection residues of pesticides is to carry out rapid sensitive to the pesticide species for suppressing certain enzyme Detection, pre-treatment is simple, detection time is short, required instrument and equipment is simple, it is adaptable to on-site measurement, the enzyme for having the disadvantage to use, Matrix and developer have certain specificity, and the condition that need to be controlled is relatively more, and are only applicable and qualitative detection.
Due to China's national situation influence, vegetables production base relatively disperses, and to keep the fresh of fruits and vegetables, is sold by harvesting to market Sell, it is desirable to which institute is short through the time, therefore does not allow great amount of samples chromatography or chemoluminescence method inspected by random samples from the market etc. Method is analyzed, if using these methods, not only needing the equipment, longer sample treatment and minute, height of costliness Testing cost, and often etc. analysis result comes out, and fruits and vegetables are not to be used, and exactly lose commodity value.It is high residual to prevent The veterinary antibiotics of poison enter resident's dining table, the inspection easily grasped in the urgent need to a kind of quick, easy, safe and reliable, grass-roots unit Survey technology.
Utility model content:
In view of the above-mentioned problems, the technical problems to be solved in the utility model is to provide a kind of quick agriculture efficiently with low cost Medicine residue analysis card.
A kind of quick residues of pesticides test card efficiently with low cost of the present utility model, it includes handle, package shelf, branch Spike, splash guard, test card body, instillation frid, observation window, enlarging lens, contrast colour atla;The right-hand member of the handle is provided with Centre inside package shelf, the package shelf is provided with splash guard, and the upper and lower sides of the splash guard are provided with test card body, It is provided with upper and lower end face on front side of the package shelf on the left of instillation frid, the instillation frid and is provided with observation window, institute The inside for stating observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla.
Preferably, the outer surface for instilling frid is provided with waterproof membrane.
Preferably, the bottom of the support feet is provided with skid resistant course.
Compared with prior art, the beneficial effects of the utility model are:Quick detection is easy to implement, and it is easy to operate, it is accurate Exactness is high, saves the time.
Brief description of the drawings:
For ease of explanation, the utility model is described in detail by following specific implementations and accompanying drawing.
Fig. 1 is structural representation of the present utility model.
In figure:1- handles;2- package shelfs;3- support feets;4- splash guards;5- test card bodies;6- instills frid;7- is observed Window;8- enlarging lens;9- contrasts colour atla.
Embodiment:
To make the purpose of this utility model, technical scheme and advantage of greater clarity, below by what is shown in accompanying drawing Specific embodiment describes the utility model.However, it should be understood that these descriptions are merely illustrative, and it is not intended to limit this reality With new scope.In addition, in the following description, the description to known features and technology is eliminated, to avoid unnecessarily mixing Confuse concept of the present utility model.
As shown in figure 1, present embodiment uses following technical scheme:It comprising handle 1, package shelf 2, support feet 3, Splash guard 4, test card body 5, instillation frid 6, observation window 7, enlarging lens 8, contrast colour atla 9;The right-hand member of the handle 1 is set There is the centre inside package shelf 2, the package shelf 2 that splash guard 4 is installed, the upper and lower sides of the splash guard 4 are provided with test Instillation frid 6 is provided with card body 5, the upper and lower end face of the front side of package shelf 2, the left side for instilling frid 6 is set There is observation window 7, the inside of the observation window 7 is provided with enlarging lens 8, and the left side of the observation window 7 is provided with contrast colour atla 9.
Further, the outer surface for instilling frid 6 is provided with waterproof membrane.
Further, the bottom of the support feet 3 is provided with skid resistant course.
The preparation method of the test card body 5 of present embodiment is:Filter paper is cut into 2cm × 0.75cm sky first The blank chromogenic substrate piece of white enzyme piece and 2.5cm × 1.5cm, is completely immersed in copolyester soybean esterase and chromogenic substrate liquid by the two respectively In, 7-10min is soaked, filter paper is taken out and is put into drying box dry (37 DEG C, 10-15min), by dried enzyme piece and colour developing Substrate tablet is pasted onto on plastics waterproofing papery bottom plate, and bottom plate is cut into specification is 4.5cm × 2.5cm rectangle, and is pasted onto On 9.0cm × 3.0cm plastic packaging counterdie, quick plastic packaging is carried out, covering paper film is pasted in the puncture site of test card, obtains into Savor organophosphorus pesticide quick measuring card.
The operation principle of present embodiment is:When in use, the waterproof membrane for instilling frid 6 is torn, then instilled Test fluid is to instilling in frid 6, and liquid is combined realization measurement with test card body 5, is realized and balanced by handle and support feet Formula is placed, and after placing 2-3 minutes, observation is realized by observation window 7, and observation is realized by enlarging lens 9 during observation, and with contrasting colour atla 9 realize contrast, it is easy to use, it is easy to operate, and can realize it is two-sided use, efficiency high.
General principle of the present utility model and principal character and advantage of the present utility model has been shown and described above.One's own profession The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification Simply illustrate principle of the present utility model, on the premise of the utility model spirit and scope are not departed from, the utility model is also Various changes and modifications are had, these changes and improvements are both fallen within the range of claimed the utility model.The utility model Claimed scope is by appended claims and its equivalent thereof.

Claims (3)

1. a kind of quick residues of pesticides test card efficiently with low cost, it is characterised in that:It includes handle, package shelf, support Pin, splash guard, test card body, instillation frid, observation window, enlarging lens, contrast colour atla;The right-hand member of the handle is provided with envelope Shelve, the centre inside the package shelf is provided with splash guard, the upper and lower sides of the splash guard are provided with test card body, institute State and instillation frid is provided with the upper and lower end face on front side of package shelf, observation window is provided with the left of the instillation frid, it is described The inside of observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla.
2. a kind of quick residues of pesticides test card efficiently with low cost according to claim 1, it is characterised in that:It is described The outer surface for instilling frid is provided with waterproof membrane.
3. a kind of quick residues of pesticides test card efficiently with low cost according to claim 1, it is characterised in that:It is described The bottom of support feet is provided with skid resistant course.
CN201720030881.0U 2017-01-10 2017-01-10 A kind of quick residues of pesticides test card efficiently with low cost Expired - Fee Related CN206369700U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720030881.0U CN206369700U (en) 2017-01-10 2017-01-10 A kind of quick residues of pesticides test card efficiently with low cost

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720030881.0U CN206369700U (en) 2017-01-10 2017-01-10 A kind of quick residues of pesticides test card efficiently with low cost

Publications (1)

Publication Number Publication Date
CN206369700U true CN206369700U (en) 2017-08-01

Family

ID=59390743

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720030881.0U Expired - Fee Related CN206369700U (en) 2017-01-10 2017-01-10 A kind of quick residues of pesticides test card efficiently with low cost

Country Status (1)

Country Link
CN (1) CN206369700U (en)

Similar Documents

Publication Publication Date Title
CN104007104B (en) Quickly detect kit and the using method thereof of organophosphorus pesticide
CN103499619B (en) A kind of acetylcholinesterase transducer production method detecting organophosphorus pesticide
Bakke et al. Development of a novel hygiene monitoring system based on the detection of total adenylate (ATP+ ADP+ AMP)
CN104597039A (en) Chemiluminescence sensing detection method for organophosphorus pesticide residues and application thereof
CN103674856B (en) Microchannel based on scanner uni colorimetric analysis is used for residues of organophosphate pesticides fast determining method
CN104198474A (en) Detection kit for measuring content of lipase in serum by colorimetric method
CN201660632U (en) Quick fruit and vegetable pesticide residue testing card
CN109916895A (en) A kind of dry chemistry reagent piece and preparation method thereof quantitative determining creatine concentration
CN103528977B (en) A kind of serum beta-hydroxybutyric acid kit and detection method thereof
CN105044101A (en) Quick detection card for pesticide residues based on naked eye visual colorimetric determination
CN103267760A (en) Method and kit for detecting illegal cooking oil
CN102778545B (en) Method for comprehensively and quickly screening multiparameters of illegal cooking oil
CN100514038C (en) Detection method of pesticide residue on fruit and vegetable and its device
CN106501250A (en) A kind of Rapid detection test strip and the method using the ELISA test strip Organophosphorus and carbamate pesticides pesticide residue
Brás et al. Microfluidic device for multiplexed detection of fungal infection biomarkers in grape cultivars
CN206369700U (en) A kind of quick residues of pesticides test card efficiently with low cost
JP5369095B2 (en) Cholinesterase inhibitory substance detection apparatus, detection kit and detection method
CN106771160A (en) The immunochromatography capillary method for quick of enrofloxacin residual in a kind of aquatic products
CN110514820A (en) A kind of portable urine test paper reducing interference
CN105806839A (en) Embryo-free rice freshness detecting reagent and method
CN100520373C (en) Quick detection paper card for pesticide residue in vegetable and preparation method therof
CN206594063U (en) A kind of broad-spectrum pesticide remains quick detection plate
CN206399840U (en) Detect the Rapid detection test strip of Organophosphorus and carbamate pesticides pesticide residue
CN107305212A (en) The immunological detection method and kit of a kind of organophosphate and carbamate pesticide
CN102778454B (en) Testing reagent for illegal cooking oil

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170801

Termination date: 20200110

CF01 Termination of patent right due to non-payment of annual fee