CN206369700U - A kind of quick residues of pesticides test card efficiently with low cost - Google Patents
A kind of quick residues of pesticides test card efficiently with low cost Download PDFInfo
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- CN206369700U CN206369700U CN201720030881.0U CN201720030881U CN206369700U CN 206369700 U CN206369700 U CN 206369700U CN 201720030881 U CN201720030881 U CN 201720030881U CN 206369700 U CN206369700 U CN 206369700U
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- test card
- residues
- quick
- frid
- observation window
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Abstract
The utility model discloses a kind of quick residues of pesticides test card efficiently with low cost, it is related to residues of pesticides technical field of measurement and test；The right-hand member of the handle is provided with package shelf, centre inside the package shelf is provided with splash guard, the upper and lower sides of the splash guard are provided with test card body, instillation frid is provided with upper and lower end face on front side of the package shelf, observation window is provided with the left of the instillation frid, the inside of the observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla；The utility model is easy to implement quick detection, and easy to operate, and the degree of accuracy is high, saves the time.
Description
Technical field：
The utility model is related to a kind of quick residues of pesticides test card efficiently with low cost, belongs to residues of pesticides test skill
Art field.
Background technology：
The method of existing detection organophosphorus pesticide residual quantity is a lot, mainly there is chromatography, immunization, chemiluminescence at present
Method and inhibiting AChE.
1st, chromatography is most classical, the most frequently used technology in residues of pesticides conventional detection.General use gas-chromatography (GC),
Liquid chromatography (LC), high performance liquid chromatography (HPLC), compounds GC-MS (GC-MS), supercritical fluid chromatography technology (SFC), exempt from
The technologies such as epidemic disease affinity chromatography technology (IAC), gel permeation chromatography technology (GPC) will need to enter by the sample strictly purified after extraction
Row detection.This method great advantage is that sensitivity is high, the degree of accuracy is good, and qualitative, the quantitative result of examined agricultural chemicals can be provided simultaneously, is carried
For arbitration foundation.But there is also to technical requirements are big compared with high, equipment investment, sample preprocessing is cumbersome, censored time length, spend
The shortcomings of cost is high.As (GC-MS) can measure the range of linearity, regression equation, coefficient correlation, the rate of recovery and the correlation of 32 kinds of agricultural chemicals
Standard deviation.
Gas chromatography is the topmost assay method of current environment sample Pesticide Residues, can be to most of residues of pesticides
Accurate Analysis is measured, measurement is accurate, is national standard pesticide residue analysis method, minimum detection output is up to ng.But selectivity and
Sensitivity is not high, and pretreatment is cumbersome, using narrow range, is only applicable to volatility agricultural chemicals.
2nd, immunoassay is a kind of using antibody as biosensors, and the materials such as compound, enzyme or protein are carried out
The analytical technology of qualitative and quantitative analysis.It is in detection grain, water fruits and vegetables, meat, milk, water and soil since 1990s
Developed rapidly in terms of the residual of earth Pesticides.Technique, the U.S. are recommended to many countries by the World Food Programme (FAO)
Immuno analytical method, chromatographic technique are classified as the major technique of pesticide residue analysis by Chemical Society jointly, its knot detected
Fruit has legal effect.It is to be combined the Detecting Pesticide side based on reflecting with specificity, the invertibity of antibody by antigen
Method, the mode of predominantly detecting is to use kit.This method has that selectivity is strong, sensitivity is high, quick, simple operation and other advantages.But
Due to, immunogene preparation complexity various by pesticide variety, immune response is with the influence in terms of specificity, its application
It is subject to certain restrictions.
3rd, inhibiting AChE is to suppress some specific biology enzymes (such as acetylcholinesterase, carboxy-lesterase) based on agricultural chemicals
Activity, and by specific chromogenic reaction, observe by the naked eye or instrument readings judged, qualitatively or quantitatively to detect sample
Product Pesticide Residues situation, the existing considerably long history of application of this method, and still have suitable use value so far, can
Residual hazard for Multiple Pesticides is determined.Target organismses enzyme can be placed in chromatographic sheet, the scraps of paper or test tube using this principle
In, enzyme is reacted with sample, residues of pesticides or residual quantity are extremely low if do not had in sample, and enzymatic activity is just not suppressed, bottom
Thing can be hydrolyzed, and hydrolysate is developed the color by with specific developer reaction solution or having color in itself；, whereas if examination
Have the remains of pesticide of higher amount in sample, enzymatic activity is suppressed by agricultural chemicals, substrate can not be hydrolyzed, so as to can not develop the color, thin-layer method and
The result judgement of paper disk method can by develop the color degree size or spot size, weigh, leaching etc. carries out qualitative or sxemiquantitative point
Analysis, colorimetric method can calculate the inhibition level of enzymatic activity using absorbance, so as to judge whether there is residual toxicity in sample.
The reaction substrate commonly used in inhibiting AChE has acetyl oxyindole, the bromo- oxyindoles of acetic acid -5-, acetic acid-α-naphthylacetate
Or carboxylate, chlorine (bromine, iodine) change acetylcholine, indophenols acetic acid esters etc..Conventional coloration method has：
1. it is acetyl and choline using acetylcholine hydrolyzation product, is made by acid-base indicator bromthymol blue and acetic acid
With, aobvious blueness when residues of pesticides are high, displaing yellow is distinguished when non agricultural chemical residuum or low persticide residue；
2. act on forming aubergine azo using substrate β naphthyl acetate hydrolysate betanaphthol and developer Fast blue B
Compound；
3. acetic acid oxyindole hydrolysate indoxyl autoxidation is utilized into indigo and aobvious blueness；
4. the color of indophenols Acetate hydrolysis product itself is utilized；
5. acetylthiocholine is changed using substrate iodine (bromine, chlorine), under AchE catalysis, the iodine (bromine, chlorine) for decomposing generation is changed
Thiocholine, with 5,5 '-two thio -2,2 ' dinitrobenzoic acids (DTNB) effect, generation 5- coloured glaze base -2- nitrobenzoic acid (Huangs
Color).
The major advantage of inhibiting AChE detection residues of pesticides is to carry out rapid sensitive to the pesticide species for suppressing certain enzyme
Detection, pre-treatment is simple, detection time is short, required instrument and equipment is simple, it is adaptable to on-site measurement, the enzyme for having the disadvantage to use,
Matrix and developer have certain specificity, and the condition that need to be controlled is relatively more, and are only applicable and qualitative detection.
Due to China's national situation influence, vegetables production base relatively disperses, and to keep the fresh of fruits and vegetables, is sold by harvesting to market
Sell, it is desirable to which institute is short through the time, therefore does not allow great amount of samples chromatography or chemoluminescence method inspected by random samples from the market etc.
Method is analyzed, if using these methods, not only needing the equipment, longer sample treatment and minute, height of costliness
Testing cost, and often etc. analysis result comes out, and fruits and vegetables are not to be used, and exactly lose commodity value.It is high residual to prevent
The veterinary antibiotics of poison enter resident's dining table, the inspection easily grasped in the urgent need to a kind of quick, easy, safe and reliable, grass-roots unit
Survey technology.
Utility model content：
In view of the above-mentioned problems, the technical problems to be solved in the utility model is to provide a kind of quick agriculture efficiently with low cost
Medicine residue analysis card.
A kind of quick residues of pesticides test card efficiently with low cost of the present utility model, it includes handle, package shelf, branch
Spike, splash guard, test card body, instillation frid, observation window, enlarging lens, contrast colour atla；The right-hand member of the handle is provided with
Centre inside package shelf, the package shelf is provided with splash guard, and the upper and lower sides of the splash guard are provided with test card body,
It is provided with upper and lower end face on front side of the package shelf on the left of instillation frid, the instillation frid and is provided with observation window, institute
The inside for stating observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla.
Preferably, the outer surface for instilling frid is provided with waterproof membrane.
Preferably, the bottom of the support feet is provided with skid resistant course.
Compared with prior art, the beneficial effects of the utility model are：Quick detection is easy to implement, and it is easy to operate, it is accurate
Exactness is high, saves the time.
Brief description of the drawings：
For ease of explanation, the utility model is described in detail by following specific implementations and accompanying drawing.
Fig. 1 is structural representation of the present utility model.
In figure：1- handles；2- package shelfs；3- support feets；4- splash guards；5- test card bodies；6- instills frid；7- is observed
Window；8- enlarging lens；9- contrasts colour atla.
Embodiment：
To make the purpose of this utility model, technical scheme and advantage of greater clarity, below by what is shown in accompanying drawing
Specific embodiment describes the utility model.However, it should be understood that these descriptions are merely illustrative, and it is not intended to limit this reality
With new scope.In addition, in the following description, the description to known features and technology is eliminated, to avoid unnecessarily mixing
Confuse concept of the present utility model.
As shown in figure 1, present embodiment uses following technical scheme：It comprising handle 1, package shelf 2, support feet 3,
Splash guard 4, test card body 5, instillation frid 6, observation window 7, enlarging lens 8, contrast colour atla 9；The right-hand member of the handle 1 is set
There is the centre inside package shelf 2, the package shelf 2 that splash guard 4 is installed, the upper and lower sides of the splash guard 4 are provided with test
Instillation frid 6 is provided with card body 5, the upper and lower end face of the front side of package shelf 2, the left side for instilling frid 6 is set
There is observation window 7, the inside of the observation window 7 is provided with enlarging lens 8, and the left side of the observation window 7 is provided with contrast colour atla 9.
Further, the outer surface for instilling frid 6 is provided with waterproof membrane.
Further, the bottom of the support feet 3 is provided with skid resistant course.
The preparation method of the test card body 5 of present embodiment is：Filter paper is cut into 2cm × 0.75cm sky first
The blank chromogenic substrate piece of white enzyme piece and 2.5cm × 1.5cm, is completely immersed in copolyester soybean esterase and chromogenic substrate liquid by the two respectively
In, 7-10min is soaked, filter paper is taken out and is put into drying box dry (37 DEG C, 10-15min), by dried enzyme piece and colour developing
Substrate tablet is pasted onto on plastics waterproofing papery bottom plate, and bottom plate is cut into specification is 4.5cm × 2.5cm rectangle, and is pasted onto
On 9.0cm × 3.0cm plastic packaging counterdie, quick plastic packaging is carried out, covering paper film is pasted in the puncture site of test card, obtains into
Savor organophosphorus pesticide quick measuring card.
The operation principle of present embodiment is：When in use, the waterproof membrane for instilling frid 6 is torn, then instilled
Test fluid is to instilling in frid 6, and liquid is combined realization measurement with test card body 5, is realized and balanced by handle and support feet
Formula is placed, and after placing 2-3 minutes, observation is realized by observation window 7, and observation is realized by enlarging lens 9 during observation, and with contrasting colour atla
9 realize contrast, it is easy to use, it is easy to operate, and can realize it is two-sided use, efficiency high.
General principle of the present utility model and principal character and advantage of the present utility model has been shown and described above.One's own profession
The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification
Simply illustrate principle of the present utility model, on the premise of the utility model spirit and scope are not departed from, the utility model is also
Various changes and modifications are had, these changes and improvements are both fallen within the range of claimed the utility model.The utility model
Claimed scope is by appended claims and its equivalent thereof.
Claims (3)
1. a kind of quick residues of pesticides test card efficiently with low cost, it is characterised in that：It includes handle, package shelf, support
Pin, splash guard, test card body, instillation frid, observation window, enlarging lens, contrast colour atla；The right-hand member of the handle is provided with envelope
Shelve, the centre inside the package shelf is provided with splash guard, the upper and lower sides of the splash guard are provided with test card body, institute
State and instillation frid is provided with the upper and lower end face on front side of package shelf, observation window is provided with the left of the instillation frid, it is described
The inside of observation window is provided with the left of enlarging lens, the observation window and is provided with contrast colour atla.
2. a kind of quick residues of pesticides test card efficiently with low cost according to claim 1, it is characterised in that：It is described
The outer surface for instilling frid is provided with waterproof membrane.
3. a kind of quick residues of pesticides test card efficiently with low cost according to claim 1, it is characterised in that：It is described
The bottom of support feet is provided with skid resistant course.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720030881.0U CN206369700U (en) | 2017-01-10 | 2017-01-10 | A kind of quick residues of pesticides test card efficiently with low cost |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720030881.0U CN206369700U (en) | 2017-01-10 | 2017-01-10 | A kind of quick residues of pesticides test card efficiently with low cost |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206369700U true CN206369700U (en) | 2017-08-01 |
Family
ID=59390743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720030881.0U Expired - Fee Related CN206369700U (en) | 2017-01-10 | 2017-01-10 | A kind of quick residues of pesticides test card efficiently with low cost |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206369700U (en) |
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2017
- 2017-01-10 CN CN201720030881.0U patent/CN206369700U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170801 Termination date: 20200110 |
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| CF01 | Termination of patent right due to non-payment of annual fee |