CN206304767U - A kind of particle separating device and system - Google Patents
A kind of particle separating device and system Download PDFInfo
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- CN206304767U CN206304767U CN201621387175.3U CN201621387175U CN206304767U CN 206304767 U CN206304767 U CN 206304767U CN 201621387175 U CN201621387175 U CN 201621387175U CN 206304767 U CN206304767 U CN 206304767U
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Abstract
The utility model discloses a kind of particle separating device and system, it is related to separate particles technical field, wherein described device to include：Fluid flow paths, including：Sample liquid entrance, for being input into the sample liquid containing particle to be separated；Sheath fluid entrance, for being input into sheath fluid；Focus channel, connects with the sample liquid entrance and the sheath fluid entrance respectively；Split tunnel, connects with the focus channel；The split tunnel is rectangle with the cross section of the focus channel；At least two particle outlets, are respectively used at least two particles after output is separated；Focused ultrasound devices, for producing the first ultrasonic wave, first ul-trasonic irradiation to make it move to the same plane vertical with the direction of propagation of first ultrasonic wave in the particle to be separated in the focus channel；Vltrasonic device is separated, for producing the second ultrasonic wave, second ul-trasonic irradiation different size of separate particles is formed the different particles beams in the particle to be separated in the split tunnel.By the utility model, separate particles of different sizes can be come.
Description
Technical field
The utility model is related to separate particles technical field, and in particular to a kind of particle separating device and system.
Background technology
Separate particles can be used for the medical in vitro diagnosis such as rare tumor cell enrichment, blood constituent separation and treat.It is super
Compared with method of the sound wave separate particles with electricity, magnetically or optically, with without pretreatment, device volume is small, be easily integrated and
The advantage such as it is miniaturized, has a wide range of application, and can be by changing the non-ultrasound such as the ultrasound parameters such as driving voltage, frequency and flow velocity
Parameter is applied to different situations, and adjustability is high.It is existing to carry out separate particles technology using ultrasonic wave, such as based on sound reduced factor
Separation method, the method mainly using the particle of positive and negative sound reduced factor in ultrasonic standing wave respectively to node and antinode
Place's motion, the method typically produces a node at the center of fluid passage, and antinode, positive sound pair are produced in the both sides of fluid passage
The particle of specific factor moves to pipeline center under acoustic radiation force effect, and the particle of negative sound reduced factor is acted in acoustic radiation force
Under move to pipeline both sides, so as to realize separate particles.But it is opposite that this method can be only used for the separation of sound reduced factor symbol
Particle, range of application is limited.
The content of the invention
In view of this, the utility model embodiment provides a kind of particle separating device and system, to solve existing utilization
Ultrasonic wave carries out the problem that the method scope of application of separate particles is limited.
According in a first aspect, the utility model embodiment provide a kind of particle separating device, including：Liquid flow is all
Road, including：Sample liquid entrance, for being input into the sample liquid containing particle to be separated；Sheath fluid entrance, for being input into sheath fluid；Focus channel,
Connected with the sample liquid entrance and the sheath fluid entrance respectively；Split tunnel, connects with the focus channel；The split tunnel
Cross section with the focus channel is rectangle；At least two particle outlets, are respectively used to after output is separated at least two
Particle；Focused ultrasound devices, for producing the first ultrasonic wave, first ul-trasonic irradiation is in treating point in the focus channel
It is set to move on the same plane vertical with the direction of propagation of first ultrasonic wave from particle；The biography of first ultrasonic wave
Broadcast direction vertical with the liquid flow direction in the focus channel；Vltrasonic device is separated, it is described for producing the second ultrasonic wave
Second ul-trasonic irradiation makes different size of separate particles form different particles in the particle to be separated in the split tunnel
Beam；The direction of propagation of second ultrasonic wave is vertical with the liquid flow direction in the split tunnel.
Alternatively, the focused ultrasound devices include the first Vltrasonic device and the second Vltrasonic device that are oppositely arranged, described
First ultrasonic wave is the standing wave of first Vltrasonic device and the ultrasound synthesis produced by second Vltrasonic device；And/or,
The Vltrasonic device that separates includes the 3rd Vltrasonic device and the 4th Vltrasonic device that are oppositely arranged, and second ultrasonic wave is described
The standing wave of the 3rd Vltrasonic device and the ultrasound synthesis produced by the 4th Vltrasonic device.
Alternatively, the width of the rectangular cross section of the focus channel meets：N1λ1=2L1；Wherein N1The first surpass for described
Sound wave amplitude in focus channel is the number of 0 point, λ1It is the wavelength of first ultrasonic wave, L1It is the focus channel
The width of rectangular cross section.
Alternatively, the width of the rectangular cross section of the split tunnel meets：N2λ2=2L2；Wherein N2The second surpass for described
Sound wave amplitude in split tunnel is the number of 0 point, λ2It is the wavelength of second ultrasonic wave, L2It is the split tunnel
The width of rectangular cross section.
Alternatively, the height of the height of the rectangular cross section of the split tunnel and the rectangular cross section of the focus channel
It is identical.
Alternatively, width of the width of the rectangular cross section of the split tunnel more than the rectangular cross section of the focus channel
Degree.
Alternatively, cross-sectional area of the cross-sectional area of the sample liquid entrance less than the sheath fluid entrance.
Alternatively, at least two particle outlet includes the first particle outlet and the second particle outlet；Described first
Subexit is the outlet of the split tunnel main body；Second particle outlet is the branch road connected with the split tunnel main body
Passage.
Alternatively, cross-sectional area of the cross-sectional area of first particle outlet more than second particle outlet.
According to second aspect, the utility model embodiment provides a kind of separate particles system, including：First aspect or
Particle separating device described in the optional mode of any one of first aspect；First syringe pump, for fixed flow rate to institute
State particle separating device sample liquid of the injection containing particle to be separated；Second syringe pump, for being divided to the particle with fixed flow rate
Sheath fluid is injected from device.
Particle separating device, system that the utility model embodiment is provided, including fluid flow paths, focusing ultrasound dress
Put and separate Vltrasonic device, wherein fluid flow paths include sample liquid entrance, sheath fluid entrance, focus channel, split tunnel and extremely
Few two particle outlets, it is made by focused ultrasound devices the first ul-trasonic irradiation of generation in the particle to be separated in focus channel
Move on the same plane vertical with the direction of propagation of the first ultrasonic wave, the second ul-trasonic irradiation is produced by separating Vltrasonic device
Different size of separate particles are made to form the different particles beams in the particle to be separated in split tunnel, so as to by grain of different sizes
Son is separated.
Brief description of the drawings
Feature and advantage of the present utility model can be more clearly understood from by reference to accompanying drawing, accompanying drawing be it is schematical without
It is interpreted as carrying out any limitation to the utility model, in the accompanying drawings：
Fig. 1 shows the top view of the particle separating device according to the utility model embodiment；
Fig. 2 shows the left view of the particle separating device according to the utility model embodiment；
It is vertical with the direction of propagation of the first ultrasonic wave same that Fig. 3 shows that particle to be separated is moved in focus channel
Top view when in plane；
Fig. 4 shows the top view of particle to be separated movement locus in particle separating device；
Fig. 5 shows top view of the particle to be separated when forming the different particles beams after focus channel and split tunnel；
Fig. 6 shows the schematic diagram of the reference axis set up in passage；
Schematic diagram when Fig. 7 shows that ultrasonic standing wave forms a node in passage；
Fig. 8 shows the power gesture distribution schematic diagram of particle to be separated；
Fig. 9 shows the schematic diagram of the acoustic radiation force size that the particle of different radii is subject in ultrasonic standing wave；
Figure 10 shows stress diagram of the particle to be separated in passage；
Figure 11 shows the relation of the time and particle size at Particles Moving to be separated to node；
Figure 12 show the separated particle to form the different particles beams from different outlet outflows when top views.
Specific embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Accompanying drawing in type embodiment, is clearly and completely described, it is clear that retouched to the technical scheme in the utility model embodiment
The embodiment stated is a part of embodiment of the utility model, rather than whole embodiments.Based on the implementation in the utility model
Example, the every other embodiment that those skilled in the art are obtained under the premise of creative work is not made belongs to this reality
With novel protected scope.
Embodiment one
The utility model embodiment provides a kind of particle separating device.Fig. 1 is shown according to the utility model embodiment
Particle separating device top view, Fig. 2 shows the left view of the particle separating device according to the utility model embodiment.Such as
Shown in Fig. 1, including fluid flow paths 20, focused ultrasound devices 30 and separation Vltrasonic device 40.
According to Fig. 1 and Fig. 2,10 is that cross section is the substrate of rectangle, groove is set on the surface of substrate 10, in the upper of groove
Square cover glass plate 50, forms fluid flow paths 20 between glass plate 50 and substrate 10.Focused ultrasound devices 30 and separation are super
Acoustic device 40 is located at the one or both sides of fluid flow paths 20, is arranged at the side of substrate 10.The width of substrate 10 and glass plate
It is 4mm.
As shown in figure 1, the fluid flow paths 20 specifically include sample liquid entrance 21, sheath fluid entrance 22, focus channel 23, point
From the particle outlet of passage 24 and at least two.In Fig. 2 20 are specifically as follows focus channel 23, the particle distribution in figure is not considered
During situation, in Fig. 2 20 can also refer to split tunnel 24.Sample liquid entrance 21 is used to be input into the sample liquid containing particle to be separated.Sheath
Liquid entrance 22 is used to be input into sheath fluid.Focus channel 23 is connected with sample liquid entrance 21 and sheath fluid entrance 22 respectively.Split tunnel 24 with
Focus channel 23 is connected, and the two is coaxially disposed.Split tunnel 24 is rectangle with the cross section of focus channel 23.At least two
Subexit is respectively used at least two particles after output is separated, such as the first particle outlet 25 and the second particle outlet 26.
The height of the rectangular cross section of split tunnel 24 is highly identical with the rectangular cross section of focus channel 23, such as Fig. 2
Shown in, it is 125 μm；Width of the width of the rectangular cross section of split tunnel 24 more than the rectangular cross section of focus channel 23
Degree, the width of the rectangular cross section of focus channel 23 is 250 μm, and the width of the rectangular cross section of split tunnel 24 is 300 μm；It is poly-
Burnt Vltrasonic device 30 is 6 ㎜ along the length of the axial direction of focus channel 23, separates length of the Vltrasonic device 40 along the axial direction of split tunnel 24
It is 3 ㎜.
Focused ultrasound devices 30, for producing the first ultrasonic wave, first ul-trasonic irradiation is in treating in focus channel 23
Separating particles make it move on the same plane vertical with the direction of propagation of the first ultrasonic wave.The direction of propagation of the first ultrasonic wave
It is vertical with the liquid flow direction in focus channel 23.Vltrasonic device 40 is separated, for producing the second ultrasonic wave, the second ultrasonic wave
Acting on the particle to be separated in split tunnel 24 makes different size of separate particles form the different particles beams.Second ultrasonic wave
The direction of propagation is vertical with the liquid flow direction in split tunnel 24.
Used as a kind of optional embodiment of the present embodiment, focused ultrasound devices 30 include the first ultrasound dress being oppositely arranged
Put 31 and second Vltrasonic device 32, the first ultrasonic wave is the first Vltrasonic device 31 and the ultrasonic wave produced by the second Vltrasonic device 32
The ultrasonic standing wave of synthesis.Separating Vltrasonic device 40 includes the 3rd Vltrasonic device 41 and the 4th Vltrasonic device 42 that are oppositely arranged, the
Two ultrasonic waves are the ultrasonic standing wave of the 3rd Vltrasonic device 41 and the ultrasound synthesis produced by the 4th Vltrasonic device 42.Alternatively,
First Vltrasonic device 31, the second Vltrasonic device 32, the 3rd Vltrasonic device 41 and the 4th Vltrasonic device 42 are ultrasonic transducer.
As shown in figure 4, the wavelength of the ultrasonic wave that control focused ultrasound devices 30 are sent, makes its shape in focus channel 23
Into ultrasonic standing wave, the frequency for for example controlling the ultrasonic wave that focused ultrasound devices 30 are sent is 6MHz, then ultrasonic standing wave is being focused on
Node number in passage 23 is 2.In the case of the velocity ratio of sheath fluid and sample liquid is suitable, sample liquid can be along the inwall of focus channel 23
Side flowing so that the lateral movement of particle to be separated inside sample liquid along the inwall of focus channel 23.Now particle to be separated
Acted on by acoustic radiation force in ultrasonic standing wave, the acoustic radiation force can be calculated according to Gorkov equations：
Wherein, FacIt is the volume (V of the acoustic radiation force that particle is subject to, its size and particlep), acoustic radiation pressure (p0), wavelength
The distance between (λ), compressibility (β), density (ρ) and particle and standing wave section x is relevant；Subscript p, m represent particle and Jie respectively
Matter；Φ (β, ρ) is defined as sound reduced factor (acoustic contrast factor), characterizes the compression of particle and surrounding medium
The difference of property and density.
Wherein, N is the node number formed in passage, and λ is wavelength, and L is channel width.
In stationary field, acoustic pressure can be expressed as
P (x, t)=p0cos(kx)cos(wt) (3)
Wherein, p (x, t) is instantaneous sound pressure；p0It is sound pressure amplitude；Represent wave number；W=2 π f represent angular frequency.
As shown in fig. 6, reference axis origin is arranged at channel center, reference axis is set up in passage.Using formula (1)
(3) acoustic radiation force that the stationary field acoustic pressure and particle formed between can calculating in passage are subject to, as shown in Figure 7.Fig. 7
Shown is one situation of node of formation in passage, and its transverse axis represents particle to be separated in the channel along the x-axis shown in Fig. 5
The position in direction, the longitudinal axis represents the size (positive and negative values represent in opposite direction) of acoustic radiation force or acoustic pressure, and solid line represents acoustic radiation force
With particle x-axis direction position change, dotted line represent acoustic pressure with particle x-axis direction position change.Positioned at negative semiaxis
The acoustic radiation force that particle is subject to is for just (sensing node), the acoustic radiation force being subject to positioned at the particle of positive axis is negative (beacon
Section), therefore, particle of the initial position not at node will be moved under acoustic radiation force effect at node, at standing wave node
Power gesture is zero, forms potential well, and Fig. 8 shows that particle to be separated is distributed along the power gesture in the x-axis direction shown in Fig. 6, and its transverse axis is represented
In the channel along the position in the x-axis direction shown in Fig. 5, the longitudinal axis represents the size of power gesture to particle to be separated.For these reasons, gather
Each particle to be separated in burnt passage can keep poised state at node, and travel forward, so as to when there is multiple particles
Can move on the same plane vertical with the direction of propagation of the first ultrasonic wave, as shown in Figure 3 and Figure 4.
According to (1) formula, the acoustic radiation force that particle is subject to is directly proportional to the volume of particle, and the volume of particle isTherefore acoustic radiation force suffered by particle is directly proportional to the cube of particle radii, so that size has the grain of fine difference
Movement locus of the son in sound field differs greatly, and the acoustic radiation force size that the particle of different radii is subject in ultrasonic standing wave is such as
Shown in Fig. 9.The acoustic radiation force that particle at node is subject to is zero, and the acoustic radiation force that the particle at antinode is subject to is maximum, and is pointed to
Node position；The acoustic radiation force that the bigger particle of radius is subject to is significantly increased.
The predominant intermolecular forces that particle is subject in fluid and stationary field are except acoustic radiation force, also gravity, buoyancy and fluid
Drag force, as shown in Figure 10.Gravity and buoyancy are cancelled out each other, and drag force can be expressed as
Fdrag=6 π η r (U-v) (4)
Wherein, η is the dynamic viscosity of fluid, and r is particle radii, and U and v is respectively the speed of fluid and particle.Can recognize
In the horizontal speed of passage it is zero for fluid, then (4) formula can be reduced to
Fdrag=-6 π η rv (5)
Acoustic radiation force and F during equal drag force according to suffered by particledrag=Fac, utilize (1) and (5 formula) to obtain
(6) formula of solution can obtain various sizes of particle and time at node is moved near channel side wall, such as Figure 11 institutes
Show, various sizes of Particles Moving to be separated is different to the time at node, thus their movement locus is different.
As shown in figure 4, control separates the wavelength of the ultrasonic wave that Vltrasonic device 40 is sent simultaneously, make it in split tunnel 24
Interior formation ultrasonic standing wave, the frequency for for example controlling to separate the ultrasonic wave that Vltrasonic device 40 is sent is 2.5MHz, then ultrasonic standing wave
Node number in split tunnel 24 is the position of node in the position of node in 1, and split tunnel 24 and focus channel 23
Put not in the same plane.In split tunnel 24, the acoustic radiation force suffered by big particle in particle to be separated is larger, and its
The time moved to needed at node is shorter, so as to big particle and small particles in split tunnel 24 in the presence of ultrasonic standing wave
It is rapidly separated, forms the different particles beams, as shown in Figure 4 and Figure 5.
As shown in Fig. 1, Fig. 4 and Figure 12, the big particle after separation is flowed out by the first particle outlet 25, and small particles are by the
Two particle outlets 26 flow out, so as to realize that the big particle in sample liquid in particle to be separated is separated with small particles.Wherein, first
Particle outlet 25 is the outlet of the main body of split tunnel 24, and the second particle outlet 26 is that the branch road connected with the main body of split tunnel 24 leads to
Road.
It should be added that, the first particle outlet 25 is not limited to be arranged on the main body of split tunnel 24, it is also possible to
It is the bypass passage of split tunnel 24；Additionally, particle outlet can have multiple, however it is not limited to 2 shown in Fig. 1, can be with
More, to isolate various particles that radius size differs larger.
Additionally, focused ultrasound devices 30, or the single ultrasonic generator of the side of focus channel 23 is only arranged at,
Such as ultrasonic transducer, the direction of propagation of the ultrasonic wave for being sent is vertical with the liquid flow direction in focus channel 23；Accordingly
Node position correspond to the position that amplitude in single ultrasonic wave is 0.Similarly, separate Vltrasonic device 40, or only set
It is placed in the single ultrasonic generator of the side of split tunnel 24, such as ultrasonic transducer, the direction of propagation of the ultrasonic wave for being sent
It is vertical with the liquid flow direction in split tunnel 24；Corresponding node position corresponds to the position that amplitude in single ultrasonic wave is 0
Put.
The first ultrasonic wave or ultrasonic standing wave produced by focused ultrasound devices 30 (are schemed along the length of the axial direction of focus channel 23
The length of ultrasonic transducer 31 and 32 in 1) determined according to the frequency of sheath flow velocity, sample liquid flow velocity and ultrasonic wave or ultrasonic standing wave,
Need to only ensure that particle to be separated is transported before reaching split tunnel 24 in the presence of the ultrasonic wave that the focused ultrasound devices 30 are sent
Move to the same plane vertical with the direction of propagation of the first ultrasonic wave.Separate the second ultrasound produced by Vltrasonic device 40
Ripple or ultrasonic standing wave along the axial direction of split tunnel 24 length (length of ultrasonic transducer 41 and 42 i.e. in figure) particle radii difference
Size and the frequency of ultrasonic wave or ultrasonic standing wave determine, the ultrasonic wave that only need to ensure to be sent in the separation Vltrasonic device 40
The different size in the particle to be separated on the same plane vertical with the direction of propagation of the first ultrasonic wave is had moved under effect
Particle reach any one particle outlet before form the different particles beams.
The width of the rectangular cross section of focus channel 23 is not limited to 250 μm shown in Fig. 1, only needs to meet：N1λ1=
2L1, wherein N1For the first ultrasonic wave, amplitude is number (or the number of the node of ultrasonic standing wave of 0 point in focus channel
Mesh), λ1It is the wavelength (or wavelength of ultrasonic standing wave) of the first ultrasonic wave, L1It is the width of the rectangular cross section of focus channel.Point
300 μm of satisfactions shown in Fig. 1 are not limited to from the width of the rectangular cross section of passage：N2λ2=2L2.Wherein N2It is
Two ultrasonic waves amplitude in split tunnel is the number (or number of the node of ultrasonic standing wave) of 0 point, λ2It is the second ultrasound
The wavelength (or wavelength of ultrasonic standing wave) of ripple, L2It is the width of the rectangular cross section of split tunnel.
Focus channel 23 can be coaxially disposed with split tunnel 24, it is also possible to not be coaxially disposed, for example the axle of the two
Line is parallel.
The width of the rectangular cross section of split tunnel can be more than or equal to the width of the rectangular cross section of focus channel.Point
It is more beneficial for quickly and accurately during the width of the rectangular cross section for being more than focus channel from the width of the rectangular cross section of passage
Different size of separate particles.Specifically, when particle to be separated moves to split tunnel from focus channel 23, originally along passage
The acoustic radiation force that the small particles of the lateral movement of inwall one are subject to is smaller, due to passage cross-sectional area increase, liquid stream effect under according to
So along the lateral movement of split tunnel inwall one；And big particle is larger due to the acoustic radiation force being subject to, it can be to the node of channel middle
Move point (or point that amplitude is 0) position.Thus, the distance between plane residing for big, lower particle can be increased quickly, so as to
It is enough to isolate different size of particle more rapidly, exactly.
The length of focus channel 23 and split tunnel 24 determines according to the flow velocity or velocity ratio of sheath fluid and sample liquid.Specifically,
The length of focus channel 23 need to ensure to be moved to and first before reaching split tunnel 24 in the presence of particle ultrasonic wave to be separated
On the vertical same plane in the direction of propagation of ultrasonic wave, the length of split tunnel 24 need to ensure the different size in particle to be separated
Particle reach any one particle outlet before form the different particles beams.
Alternatively, the cross-sectional area of the first particle outlet 25 more than the second particle outlet 26 cross-sectional area, less the
Two particle outlets cause that the liquid stream of outflow is thinner, it is to avoid big particle changes the direction of motion due to liquid rheology and flows into the second particle
Outlet, i.e., more precisely isolate small particles.
Alternatively, the cross-sectional area of sample liquid entrance 21 can cause focus channel less than the cross-sectional area of sheath fluid entrance 22
Interior sheath flow quantity is more than sample liquid flow, so that the particle to be separated in sample liquid can more press close to vias inner walls side.
Embodiment two
The utility model embodiment provides a kind of separate particles system, as shown in figure 1, including embodiment one or embodiment
Particle separating device described in one any one optional embodiment.Additionally, also including the first syringe pump 60 and the second injection
Pump 70.
First syringe pump 60 is used for fixed flow rate to particle separating device sample liquid of the injection containing particle to be separated.Second
Syringe pump 70 is used to inject sheath fluid to particle separating device with fixed flow rate.The flow velocity of sample liquid and the flow velocity of sheath fluid can with identical,
Can also be different.Alternatively, the flow velocity of sheath fluid is quickly moved more than the particle to be separated that the flow velocity of sample liquid is more beneficial in sample liquid
On to the same plane vertical with the direction of propagation of the first ultrasonic wave, such as the two ratio is 5：3.
Embodiment three
The utility model embodiment provides any one optional embodiment party of a kind of use embodiment one or embodiment one
Particle separating device described in formula, or the method that separate particles system described in embodiment two carries out separate particles, the method
Comprise the following steps：
S10：Regulation focused ultrasound devices produce the first ultrasonic wave, and adjust the second ultrasound for separating Vltrasonic device generation
Ripple, and the point that the first ultrasonic amplitude is 0 is formed the different particles beams from the point that the second ultrasonic amplitude is 0.Alternatively, second
Wavelength of the wavelength of ultrasonic wave more than the first ultrasonic wave.
Second ultrasonic amplitude is by 0 point (or ultrasonic standing wave) plane for being formed and the point that the first ultrasonic amplitude is 0
The plane that (or ultrasonic standing wave) is formed can make particle to be separated be shaken to ultrasonic wave in the presence of acoustic radiation force for Different Plane
Width is 0 point motion, so as to realize separate particles.Second ultrasonic wave differs bigger with the wavelength of the first ultrasonic wave, two planes
The distance between it is bigger, be more beneficial for separate particles to be separated accurately and rapidly.
S20：Sample liquid containing particle to be separated is input into by sample liquid entrance, while being input into sheath fluid by sheath fluid entrance, is made
Particle to be separated in sample liquid in focus channel can move to it is vertical with the direction of propagation of the first ultrasonic wave same flat
On face, and the plane presses close to vias inner walls side.
Although being described in conjunction with the accompanying embodiment of the present utility model, those skilled in the art can not depart from this
Various modification can be adapted in the case of the spirit and scope of utility model and modification, and such modification and modification are each fallen within by appended power
Profit is required within limited range.
Claims (10)
1. a kind of particle separating device, it is characterised in that including：
Fluid flow paths, including：
Sample liquid entrance, for being input into the sample liquid containing particle to be separated；
Sheath fluid entrance, for being input into sheath fluid；
Focus channel, connects with the sample liquid entrance and the sheath fluid entrance respectively；
Split tunnel, connects with the focus channel；The split tunnel is rectangle with the cross section of the focus channel；
At least two particle outlets, are respectively used at least two particles after output is separated；
Focused ultrasound devices, for producing the first ultrasonic wave, first ul-trasonic irradiation is in treating point in the focus channel
It is set to move on the same plane vertical with the direction of propagation of first ultrasonic wave from particle；The biography of first ultrasonic wave
Broadcast direction vertical with the liquid flow direction in the focus channel；
Vltrasonic device is separated, for producing the second ultrasonic wave, second ul-trasonic irradiation is in treating point in the split tunnel
From particle, different size of separate particles are made to form the different particles beams；The direction of propagation of second ultrasonic wave separates with described
Liquid flow direction in passage is vertical.
2. particle separating device according to claim 1, it is characterised in that the focused ultrasound devices include being oppositely arranged
The first Vltrasonic device and the second Vltrasonic device, first ultrasonic wave be first Vltrasonic device and described second ultrasound dress
The standing wave of the ultrasound synthesis produced by putting；And/or,
The Vltrasonic device that separates includes the 3rd Vltrasonic device and the 4th Vltrasonic device that are oppositely arranged, and second ultrasonic wave is
The standing wave of the 3rd Vltrasonic device and the ultrasound synthesis produced by the 4th Vltrasonic device.
3. particle separating device according to claim 1, it is characterised in that the width of the rectangular cross section of the focus channel
Degree meets：N1λ1=2L1；Wherein N1It is the number of point that the first ultrasonic wave amplitude in focus channel is 0, λ1It is described
The wavelength of one ultrasonic wave, L1It is the width of the rectangular cross section of the focus channel.
4. the particle separating device according to claim 1 or 3, it is characterised in that the rectangular cross section of the split tunnel
Width meet：N2λ2=2L2；Wherein N2It is the number of point that the second ultrasonic wave amplitude in split tunnel is 0, λ2For institute
State the wavelength of the second ultrasonic wave, L2It is the width of the rectangular cross section of the split tunnel.
5. particle separating device according to claim 1, it is characterised in that the height of the rectangular cross section of the split tunnel
Degree is highly identical with the rectangular cross section of the focus channel.
6. particle separating device according to claim 5, it is characterised in that the width of the rectangular cross section of the split tunnel
Width of the degree more than the rectangular cross section of the focus channel.
7. particle separating device according to claim 1, it is characterised in that the cross-sectional area of the sample liquid entrance is less than institute
State the cross-sectional area of sheath fluid entrance.
8. particle separating device according to claim 1, it is characterised in that at least two particle outlet includes first
Particle outlet and the second particle outlet；
First particle outlet is the outlet of the split tunnel main body；Second particle outlet is and the split tunnel
The bypass passage of main body connection.
9. particle separating device according to claim 8, it is characterised in that the cross-sectional area of first particle outlet is big
In the cross-sectional area of second particle outlet.
10. a kind of separate particles system, it is characterised in that including：
Particle separating device described in any one of claim 1 to 9；
First syringe pump, the sample liquid for containing particle to be separated to particle separating device injection with fixed flow rate；
Second syringe pump, for injecting sheath fluid to the particle separating device with fixed flow rate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106853381A (en) * | 2016-12-16 | 2017-06-16 | 苏州国科昂卓医疗科技有限公司 | A kind of particle separating device, system and separate particles method |
| CN114345427A (en) * | 2020-10-14 | 2022-04-15 | 精工爱普生株式会社 | Fluid device |
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2016
- 2016-12-16 CN CN201621387175.3U patent/CN206304767U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106853381A (en) * | 2016-12-16 | 2017-06-16 | 苏州国科昂卓医疗科技有限公司 | A kind of particle separating device, system and separate particles method |
| CN106853381B (en) * | 2016-12-16 | 2024-02-02 | 苏州国科昂卓医疗科技有限公司 | Particle separation device, system and particle separation method |
| CN114345427A (en) * | 2020-10-14 | 2022-04-15 | 精工爱普生株式会社 | Fluid device |
| CN114345427B (en) * | 2020-10-14 | 2024-03-19 | 精工爱普生株式会社 | Fluid device |
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