CN206285290U - The organization engineering skin that Sodium Alginate Hydrogel Films support builds - Google Patents
The organization engineering skin that Sodium Alginate Hydrogel Films support builds Download PDFInfo
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- CN206285290U CN206285290U CN201621027284.4U CN201621027284U CN206285290U CN 206285290 U CN206285290 U CN 206285290U CN 201621027284 U CN201621027284 U CN 201621027284U CN 206285290 U CN206285290 U CN 206285290U
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- sodium alginate
- skin
- cell
- alginate hydrogel
- hydrogel films
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Abstract
The utility model discloses the organization engineering skin that a kind of Sodium Alginate Hydrogel Films support builds, it is made up of epidermis and skin corium from top to bottom, epidermis is to be located at one layer of seed cell above skin corium, skin corium includes seed cell and Sodium Alginate Hydrogel Films support, seed cell is located in the surface and support of Sodium Alginate Hydrogel Films support, to form cutaneous appendages.Due to containing large quantity of moisture in hydrogel scaffold cross-linked network, cell nutrients can well be supplied, EGF has been crosslinked it simultaneously, the growth and differentiation of cell can be adjusted, therefore hydrogel scaffold can preferably needed for analog cell growth class loading sample physics and space structure, namely there is provided the dimensional culture space of cell, the culture and function for being more beneficial for Epidermal basal layer cells are maintained, and build the engineered skin substitute with bioactivity.The utility model is applied when on the surface of a wound, the surface of a wound can be helped to recover normal institutional framework.
Description
Technical field
The utility model is related to artificial skin dressing field.
Background technology
Skin is the maximum organ of people's bulk area, is important protective barrier, also with sensation, protection, reconciles body temperature etc.
Important physiological function.When normal skin tissue, in extraneous Risk Factors (burn, wound etc.) and body internal factor, (nutrition is not
Good, anoxic and infection etc.) effect caused by infringement form the surface of a wound, show as destruction, the missing of normal structure of skin integrity
With the forfeiture of skin function.
Clinically the common surface of a wound has the major diseases such as burn, wound, scar, Skin pigment abnormalities, benign malignant tumour
Deng acute wounds such as the defects of skin formed after surgery excision, also burst just like diabetic ulcer, vascular ulcers, pressure
The chronic wounds such as ulcer, radiation ulcer, neoplastic ulcer, cicatricial ulcer, neurogenic ulcer.
Operation repair be at present treat the surface of a wound main method, but, either traditional grafts, skin flap transplantation,
Stamp skin grafting dermepenthesis, wear debris emission, netted skin grafting dermepenthesis, Composite skin etc., or current organization engineering skin, at present all not
Can effectively solve the problem that the problem of wound repair.Especially often scar proliferation after major disease fire victim rehabilitation, sweat gland missing,
Skin loses original function, and quality of life is low；Chronic wound then protracted course of disease, the lighter greatly consumes medical resource, severe one
The serious harm life such as accompanying infection, septicopyemia.
Utility model content
The utility model purpose is to provide a kind of organization engineering skin, to solve at least one of above-mentioned technical problem,
That is to say and solve the problems, such as that the above-mentioned wound repair for referring to is difficult.
Another purpose of the present utility model is the preparation method for providing above-mentioned organization engineering skin, to solve above-mentioned skill
At least one of art problem.
According to one side of the present utility model, there is provided the organizational project skin that a kind of Sodium Alginate Hydrogel Films support builds
Skin, is made up of epidermis and skin corium from top to bottom,
Epidermis is to be located at one layer of seed cell above skin corium,
Skin corium includes seed cell and Sodium Alginate Hydrogel Films support, and seed cell is located at Sodium Alginate Hydrogel Films support
Surface and support in, to form cutaneous appendages.
In some specific embodiments, above-mentioned seed cell can be epidermal stem cells or be obtained by artificial culture
Epidermal basal layer cells suspension.
In some specific embodiments, above-mentioned Epidermal basal layer cells suspension can be prepared by the following method
Arrive：Artificial skin graft is taken, skin graft is positioned in digestion enzyme solutions and is digested 15~25 minutes, then autologous skin graft is taken out, and be put into
Into sodium lactate Ringer ' solution, enzyme reaction is neutralized, the epidermis of skin graft of tearing, the scraping basal layer cell on skin graft basalis is used
Sodium lactate Ringer ' solution rinses the basal layer cell collected under hanging, and Epidermal basal layer cells suspension is obtained.
In some specific embodiments, Sodium Alginate Hydrogel Films support can be prepared in the following manner：
(1) it is shown below, benzene-dioxane that the BLG-NCA monomers of 4~6g are dissolved in 80~100mL is mixed molten
In agent, with omega-amino terminated polyethylene glycol as initiator, reaction solution is poured into absolute ethyl alcohol and sunk by room temperature reaction 60~72 hours
Form sediment, filtering is vacuum dried to obtain faint yellow translucent solid, and (PEG i.e. polyethylene glycol, PBLG is poly- γ-benzyl to obtain PEG-PBLG
Pidolidone) block copolymer,
Benzene and the volume ratio of dioxane are 4 in benzene-dioxane mixed solvent:1, BLG-NCA monomer with it is omega-amino terminated
The mol ratio of polyethylene glycol is 70~80:1, reaction solution is 1 with the volume ratio of absolute ethyl alcohol:20~30；
(2) it is the PEG-PBLG block copolymers obtained by step (1) is soluble in water, make its concentration more than 1g/20ml, stirring
Lower addition EGF (EGF) stostes reach maximum capacity to the EGF in solution, 4~12 hours are stood, make EGF
It is embedded in the ball shaped nano grain of PEG-PBLG formation；
Hydrophilic PEG and hydrophobic PBLG is compounded to form amphipathic nature polyalcohol, is aligned automatically in aqueous medium,
When higher than finite concentration (1g/20ml), tight clusters are in interior between PBLG, and PEG then unfolds in outer, is self-assembly of nanometer
The ball shaped nano grain of level size.During nanoparticle is formed, EGF occurs to divide between hydrophilic medium and the hydrophobic microcells of PBLG
Match somebody with somebody, major part is embedded in the core of nanoparticle, generation is loaded with the PEG-PBLG nanoparticles of EGF.
(3) toward adding in step (2) resulting solution EGF stostes to reach maximum capacity to the EGF in solution, by 0.02
Toward sodium alginate is added in step (2) resulting solution, stirring is completely dissolved it to the ratio of~0.04g sodium alginates/mL solution,
Then the ratio in 0.04~0.1g ethylenediamines/mL solution is toward ethylenediamine is added in step (2) resulting solution, stirred at 40 DEG C to
It is completely dissolved, 2 DEG C of -6 DEG C of left undisturbed overnights obtain Sodium Alginate Hydrogel Films support.
According to another aspect of the present utility model, the utility model additionally provides above-mentioned Sodium Alginate Hydrogel Films support
The preparation method of the organization engineering skin of structure, comprises the following steps：
(1) the BLG-NCA monomers of 4~6g are dissolved in the benzene of 80~100mL-dioxane mixed solvent, with omega end
Amino-polyethyleneglycols are initiator, and reaction solution is poured into absolute ethyl alcohol and precipitated by room temperature reaction 60~72 hours, filtering, vacuum
Dry faint yellow translucent solid, obtains PEG-PBLG block copolymers,
Benzene and the volume ratio of dioxane are 4 in benzene-dioxane mixed solvent:1, BLG-NCA monomer with it is omega-amino terminated
The mol ratio of polyethylene glycol is 70~80:1, reaction solution is 1 with the volume ratio of absolute ethyl alcohol:20~30；
(2) it is the PEG-PBLG block copolymers obtained by step (1) is soluble in water, make its concentration more than 1g/20ml, stirring
Lower addition EGF stostes reach maximum capacity to the EGF in solution, 4~12 hours are stood, EGF is embedded in PEG-PBLG
In the ball shaped nano grain of formation；
(3) toward adding in step (2) resulting solution EGF stostes to reach maximum capacity to the EGF in solution, by 0.02
Toward sodium alginate is added in step (2) resulting solution, stirring is completely dissolved it to the ratio of~0.04g sodium alginates/mL solution,
Then the ratio in 0.04~0.1g ethylenediamines/mL solution is toward ethylenediamine is added in step (2) resulting solution, stirred at 40 DEG C to
It is completely dissolved, 2 DEG C of -6 DEG C of left undisturbed overnights obtain Sodium Alginate Hydrogel Films support；
(4) by 105Individual cell/cm3The ratio of Sodium Alginate Hydrogel Films support is toward the sodium alginate water obtained in step (3)
Epidermal stem cells or sprinkle Epidermal basal layer cells suspension are added in gel stent.
Compared compared to existing organization engineering skin or traditional autologous skin-grafting, organizational project skin of the present utility model
Skin has following advantages：
1. seed cell (epidermal stem cells or Epidermal basal layer cells) is placed in Sodium Alginate Hydrogel Films by the utility model
In support or on support, the engineered skin substitute with bioactivity is built.Due to seed cell and sodium alginate water
Gel stent forms cutaneous appendages, and the utility model is applied when on the surface of a wound, and the surface of a wound can be helped to recover normal knot of tissue
Structure.
2. due to containing large quantity of moisture in hydrogel scaffold cross-linked network, cell nutrients can well be supplied, while handing over
EGF is joined, the growth and differentiation of cell, therefore the class that hydrogel scaffold can preferably needed for analog cell growth can have been adjusted
Tissue sample physics and space structure, that is, there is provided the dimensional culture space of cell, it is more beneficial for Epidermal basal layer cells
Culture and function are maintained, and plasticity is high, manufacture craft is relatively easy, clinical practice is convenient.
3. organization engineering skin of the present utility model, makes quick, and process is simple can be mass, and can effectively alleviate and face
The not enough problem in skin source in bed.
Brief description of the drawings
Fig. 1 is the structural representation of organization engineering skin.
Specific embodiment
The utility model is described in further detail with reference to specific embodiment.
(1) it is shown below, benzene-dioxane that the BLG-NCA monomers of 4~6g are dissolved in 80~100mL is mixed molten
In agent, with omega-amino terminated polyethylene glycol as initiator, reaction solution is poured into absolute ethyl alcohol and sunk by room temperature reaction 60~72 hours
Form sediment, filtering is vacuum dried to obtain faint yellow translucent solid, and (PEG i.e. polyethylene glycol, PBLG is poly- γ-benzyl to obtain PEG-PBLG
Pidolidone) block copolymer,
Benzene and the volume ratio of dioxane are 4 in benzene-dioxane mixed solvent:1, BLG-NCA monomer with it is omega-amino terminated
The mol ratio of polyethylene glycol is 70~80:1, reaction solution is 1 with the volume ratio of absolute ethyl alcohol:20~30；
(2) it is the PEG-PBLG block copolymers obtained by step (1) is soluble in water, make its concentration more than 1g/20ml, stirring
Lower addition EGF (EGF) stostes reach maximum capacity to the EGF in solution, 4~12 hours are stood, make EGF
It is embedded in the ball shaped nano grain of PEG-PBLG formation；
Hydrophilic PEG and hydrophobic PBLG is compounded to form amphipathic nature polyalcohol, is aligned automatically in aqueous medium,
When higher than finite concentration (1g/20ml), tight clusters are in interior between PBLG, and PEG then unfolds in outer, is self-assembly of nanometer
The ball shaped nano grain of level size.During nanoparticle is formed, EGF occurs to divide between hydrophilic medium and the hydrophobic microcells of PBLG
Match somebody with somebody, major part is embedded in the core of nanoparticle, generation is loaded with the PEG-PBLG nanoparticles of EGF.
(3) toward adding in step (2) resulting solution EGF stostes to reach maximum capacity to the EGF in solution, by 0.02
Toward sodium alginate is added in step (2) resulting solution, stirring is completely dissolved it to the ratio of~0.04g sodium alginates/mL solution,
Then the ratio in 0.04~0.1g ethylenediamines/mL solution is toward ethylenediamine is added in step (2) resulting solution, stirred at 40 DEG C to
It is completely dissolved, 2 DEG C of -6 DEG C of left undisturbed overnights obtain Sodium Alginate Hydrogel Films support.
(4) by 105Individual cell/cm3The ratio of Sodium Alginate Hydrogel Films support is toward the sodium alginate water obtained in step (3)
Epidermal stem cells or sprinkle Epidermal basal layer cells suspension are added in gel stent.
Thus, cell forms organization engineering skin as shown in Figure 1 after outgrowth, from top to bottom by epidermis 1 and very
Cortex 2 is constituted.In the surface and support of Sodium Alginate Hydrogel Films support, cutaneous appendages 3 is formed with.
Due to containing large quantity of moisture in hydrogel scaffold cross-linked network, cell nutrients can be well supplied, while crosslinking
EGF, can adjust the growth and differentiation of cell, therefore the class group that hydrogel scaffold can preferably needed for analog cell growth
Sample physics and space structure are knitted, that is, there is provided the dimensional culture space of cell, is more beneficial for the training of Epidermal basal layer cells
Support and function is maintained, build the engineered skin substitute with bioactivity.The utility model is applied when on the surface of a wound, can
To help the surface of a wound to recover normal institutional framework.
The above is only some implementation methods of the present utility model.For the person of ordinary skill of the art, not
On the premise of departing from the utility model creation design, various modifications and improvements can be made, these belong to the utility model
Protection domain.
Claims (2)
1. the organization engineering skin that Sodium Alginate Hydrogel Films support builds, it is characterised in that from top to bottom by epidermis and corium
Layer composition,
Described epidermis is to be located at one layer of seed cell above skin corium,
Described skin corium includes seed cell and Sodium Alginate Hydrogel Films support, and described seed cell is located at sodium alginate water
In the surface and support of gel stent, to form cutaneous appendages.
2. the organization engineering skin that Sodium Alginate Hydrogel Films support according to claim 1 builds, it is characterised in that described
Seed cell is epidermal stem cells or by manually cultivating the Epidermal basal layer cells suspension for obtaining.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110402A (en) * | 2016-08-31 | 2016-11-16 | 朱家源 | Organization engineering skin that Sodium Alginate Hydrogel Films support builds and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110402A (en) * | 2016-08-31 | 2016-11-16 | 朱家源 | Organization engineering skin that Sodium Alginate Hydrogel Films support builds and preparation method thereof |
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| TR01 | Transfer of patent right |
Effective date of registration: 20190718 Address after: 510000 No. two, No. 58, Zhongshan Road, Guangdong, Guangzhou Patentee after: No.1 Hospital Affiliated to Zhongshan Univ. Address before: 510080 No. 58 Zhongshan Second Road, Guangzhou, Guangdong Province Co-patentee before: Hu Zhicheng Patentee before: Zhu Jiayuan |