CN206208905U - A kind of Potyvirus Rapid detection test strip - Google Patents
A kind of Potyvirus Rapid detection test strip Download PDFInfo
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- CN206208905U CN206208905U CN201621330258.9U CN201621330258U CN206208905U CN 206208905 U CN206208905 U CN 206208905U CN 201621330258 U CN201621330258 U CN 201621330258U CN 206208905 U CN206208905 U CN 206208905U
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Abstract
The utility model discloses a kind of Potyvirus Rapid detection test strip, including sample pad, gold standard pad, NC films, detection line T lines, nature controlling line C line, adsorptive pads and PVC base plates, Potyvirus rabbit polyclonal antibody is coated with detection line T lines, Potyvirus goat anti-rabbit antibody is coated with nature controlling line C line, colloid gold label thing is combined with gold standard pad.Potyvirus includes PVX, PVYc、PVYNTN、PVYo, PLRV, PVS, PVM, PVA, TMV and tomato black ring virus.The utility model can realize the detection to Potyvirus high specific, high sensitivity, high accuracy, high stability in sample, quick detection goes out potato virus disease, and a situation arises, is that the deciding grade and level of potato detoxicating potato potato seed rank and the prevention and control of Potyvirus provide theoretical foundation.
Description
Technical field
The utility model relates to the use of immunochromatography technique detection plant virus technical field, and in particular to a kind of potato
Viral Rapid detection test strip.
Background technology
Potato is China's application value industrial crops higher, and oneself is listed in one of big crop of China 7, is also Western China
The most important crop varieties in portion and main column support type industry, growth momentum are good, but current China potato per unit area yield is much
Less than developed country, Potyvirus is to cause that potato yield is low, ropy main cause.In Chinese medical test such as
Early pregnancy, hepatitis B, parasite, the inspection of venereal disease and the inspection of animal epidemic for example Poultry diseases, disease of domestic animals, MHV,
There is the application of immuno-chromatographic test paper strip in pigs trichina disease, the diagnosis of dog disease virus；In tobacco, pumpkin, lettuce, cucumber mosaic virus
Poison, annulus zonatus, nepovirus, Tobacco Vein Banding Caused by Potato Virus Y poison, citrus processing, cucurbit coe virus, lettuce clump branch are planted
The application of existing immuno-chromatographic test paper strip in the detection of substance, but also without field rapid detection test paper in potato crop
Application.
The technology of conventional detection Potyvirus is double antibody enzyme-linked immunosorbent assays method, and the method needs special
The technical staff of door is tested in special laboratory using special equipment, and operating process is cumbersome, and experimental period is long：1.5-
2 days.Field sampling is unable to, scene goes out result.Generally prevent and treat the danger of virosis in production using virus-free seed potato at present
Evil, to ensure the detoxification quality of potato primary stock, original seed, sets up quick, accurate, sensitive, efficient Potyvirus class disease
Malicious detection technique is the key technique of seed potato production.
Patent publication us related to this patent as follows are found by retrieving：
1st, the patent of Publication No. CN 103757138B discloses the beautiful potato of a kind of Yunnan Potato main breed 6, cloud potato
401st, marmor upsilon, potato virus X, marmor solani and corium solani 4 kinds of viruses in a surname No. 2 diseased plants of potato
Detection method.The utility model is by Ma Ling in the beautiful potato of Yunnan Potato main breed 6, cloud potato 401, a surname No. 2 diseased plants of potato
Disease in the sick sample of potato Y virus, potato virus X, marmor solani and the species specificity mixed antibody of corium solani 4 trapping
Poison, it is not necessary to which extracting sample total serum IgE carries out random primer reverse transcription and specificity virus primer composite PCR reaction.This method will be fast
Fast, special immunoprecipitation is combined with sensitive multiplex RT -PCR, while setting healthy potato random primer cDNA product phases
Virus PCR specific fragment positive plasmid is answered to be compareed as positive and negative, so as to quickly, accurately, sensitively be applied to detect field horse
The aspects such as bell potato Plant samples, antiviral material and the doubtful source of infection of field conditions.
2nd, the patent of Publication No. CN 103757138B is related to the dual PCR Simultaneous Detection of Potato Y of RT mono- diseases of two-step method
Poison, corium solani, the PCR Simultaneous Detection of Potato X viruses of triple RT mono-, marmor solani, the side of potato virus S
Method and detection kit, the synchronous inspection of one kind for being specific to 5 kinds of larger Potyviruses of Potato Industry influence and setting up
Survey 2 or 3 kind of fast method of virus, detection time only uses 4h, and sensitivity is high, high specificity, reliable and stable.The method overcomes
It is existing to potato virus disease according to semiotics routine diagnostic method False Rate is higher, serological method is difficult to detect dormancy
The shortcomings of virus and viral level less bast virus-corium solani in potato;The method is applied to horse
The early diagnosis of bell potato seedling, the various virosis of potato seed, has important to seed potato quality-monitoring and virosis prevention
Effect.
By contrast, there is the difference of essence with above-mentioned patent publication us in present patent application.
The content of the invention
Technical problem to be solved in the utility model be in order to overcome the shortcomings of existing method, Potyvirus is quick
Test strip is applied in Potato viruses detection, is capable of achieving to Potyvirus high specific, high sensitivity, height in sample
The detection of accuracy, high stability, quick detection goes out potato virus disease, and a situation arises, is potato detoxicating potato potato seed rank
Deciding grade and level and Potyvirus prevention and control provide theoretical foundation.
The purpose of this utility model is achieved through the following technical solutions：
A kind of Potyvirus Rapid detection test strip, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, matter
Control line C lines 5, adsorptive pads 6 and PVC base plates 7, the top of the PVC base plates 7 are fitted with NC films 3, the both upper ends thereof of NC films 3 point
Gold standard pad 2 and adsorptive pads 6 are not overlapped with, and the top of the gold standard pad 2 is overlapped with sample pad 1, and the detection line T lines 4 are arranged on and lean on
On the surface of NC films 3 of nearly sample pad 1, the nature controlling line C line 5 is positioned close on the NC films 3 of adsorptive pads 6, the detection line T
Potyvirus rabbit polyclonal antibody is coated with line 4, Potyvirus goat anti-rabbit antibody is coated with the nature controlling line C line 5,
Colloid gold label thing is combined with described gold standard pad 2.
And, get stuck 8,8 upper faces and the sample of getting stuck are additionally provided with outside Potyvirus Rapid detection test strip
The corresponding position of pad 1 is provided with well 9, and 8 upper faces that get stuck are provided with observation groove with the corresponding position of the NC films 3
10。
And, the NC films 3 are 1-2mm, the length that the NC films 3 are overlapped with adsorptive pads 6 with the length of the overlap joint of gold standard pad 2
It is 2-3mm to spend, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
And, the Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5, it is coated on detection line T lines 4
Potyvirus rabbit polyclonal antibody and bag be bonded to Potyvirus colloid gold label thing in gold standard pad 2, Ke Yishi
Below combine in any one：
Combination 1：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVX goat anti-rabbit antibodies, bag
It is PVX rabbit polyclonal antibodies by the Potyvirus rabbit polyclonal antibody on detection line T lines 4, the combination is in gold standard pad 2
Potyvirus colloid gold label thing be PVX colloid gold label things；
Combination 2：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVYcGoat anti-rabbit antibody, institute
It is PVY to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4cRabbit polyclonal antibody, the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PVYcColloid gold label thing；
Combination 3：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVYNTNGoat anti-rabbit antibody,
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4 is PVYNTNRabbit polyclonal antibody, the combination exists
Potyvirus colloid gold label thing in gold standard pad 2 is PVYNTNColloid gold label thing；
Combination 4：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVYoGoat anti-rabbit antibody, institute
It is PVY to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4oRabbit polyclonal antibody, the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PVYoColloid gold label thing；
Combination 5：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PLRV goat anti-rabbit antibodies, institute
It is PLRV rabbit polyclonal antibodies to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4, and the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PLRV colloid gold label things；
Combination 6：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVS goat anti-rabbit antibodies, institute
It is PVS rabbit polyclonal antibodies to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4, and the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PVS colloid gold label things；
Combination 7：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVM goat anti-rabbit antibodies, institute
It is PVM rabbit polyclonal antibodies to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4, and the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PVM colloid gold label things；
Combination 8：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is PVA goat anti-rabbit antibodies, institute
It is PVA rabbit polyclonal antibodies to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4, and the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is PVA colloid gold label things；
Combination 9：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is TMV goat anti-rabbit antibodies, institute
It is TMV rabbit polyclonal antibodies to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4, and the combination is marked in gold
Potyvirus colloid gold label thing on pad 2 is TMV colloid gold label things；
Combination 10：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line 5 is tomato black ring virus goat-anti
Rabbit antibody, the Potyvirus rabbit polyclonal antibody being coated on detection line T lines 4 is that tomato black ring virus rabbit polyclonal resists
Body, Potyvirus colloid gold label thing of the combination in gold standard pad 2 is tomato black ring virus colloid gold label thing.
In the utility model：PVX represents potato virus X, PVYcRepresent the C strains of marmor upsilon, PVYNTNRepresent
The NTN strains of marmor upsilon, PVYoThe O strains of marmor upsilon are represented, PLRV represents corium solani, PVS generations
Table potato virus S, PVM represents marmor angliae, and PVA represents marmor solani, and TMV represents tobacco mosaic virus (TMV).
Cleaning Principle of the present utility model：Collaurum mark is obtained from colloid gold label Potyvirus rabbit polyclonal antibody
Note thing, is sprayed in gold standard pad；Coating can occur the potato of immune response with purpose thing albumen composition on detection line T lines
Viral rabbit polyclonal antibody, is coated with Potyvirus goat anti-rabbit antibody, as instruction line in nature controlling line C line.When handle treats sample
After product are loaded onto in sample pad, potato disease toxalbumin forms immune compound with the colloid gold label thing in gold standard pad in sample
Thing, due to capillary effect, the compound is to the swimming of adsorptive pads direction, this compound and the Ma Ling being coated on detection line T lines
There is specific immunity association reaction in potato virus rabbit polyclonal antibody, form colloid gold label thing, antigen（Antigen refers to potato
Virus）, rabbit polyclonal antibody triplet compound and be trapped within detection line T lines, be progressively enriched with to form deeper aubergine
Band；Due to capillary effect continuation swimming forward, colloid gold label thing and the Potyvirus sheep being coated in nature controlling line C line
There is specific immune response and be trapped in anti-rabbit antibody, be progressively enriched with and deeper aubergine band is formed in nature controlling line C line,
Unnecessary uncombined material continues to chromatograph onto adsorptive pads, therefore purplish red colo(u)r streak all occurs in detection line T lines and nature controlling line C line
Be judged to positive findings；If not containing Potyvirus in sample, when colloid gold label thing reaches detection line T lines, not with coating
There is immune response in the Potyvirus rabbit polyclonal antibody on detection line T lines, therefore do not occur showing at detection line T lines
Colour band, and colloid gold label thing continues swimming forward and occurs with the Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line
Special immune response and be trapped, be progressively enriched with and purplish red colo(u)r streak formed on nature controlling line, therefore only occur in Quality Control purplish red
Line is judged to negative findings.Detection line T lines and nature controlling line C line do not manifest purplish red colo(u)r streak or other phenomenons occur, point out test strips
Failure, detection failure.
The beneficial effects of the utility model：
（1）The utility model first by immunochromatography technique be applied to potato virus disease detection, realize high specific,
Highly sensitive detection performance.
（1）The utility model is easy to use, strong applicability：Without laboratory, do not need any specific apparatus, without specialty
Human users' training, field, potato storing cellar, customs etc. be any to have the place of potato can test, each Ma Ling
Planting potato person can use.
（2）The utility model detection time is short, it is only necessary to can draw testing result within 1-5 minutes.
（3）The utility model accuracy is high：The accuracy rate and DASELISA of the ELISA test strip potato samples
The accuracy rate of immunoadsorption assay method fits like a glove, and coincidence rate is up to 100%.
（4）The utility model high specificity：A kind of only this kind virus-positive material tests line of test strips of virus of potato
And obvious band occurs in nature controlling line, there is band in other positive materials only nature controlling line, and band does not occur in detection line；Potato disease
The test strip of poison, to healthy plant and the testing result of buffer control, there is aubergine in only C lines, and T lines do not occur purplish red
Color, result is negative, therefore this test strips has specificity.
（5）The utility model stability is strong：From the point of view of test strips test result, by test strips be placed in be allowed to dry the close of drying prescription
In envelope aluminium foil bag, color slightly weakens after being preserved for a long time at 37 DEG C, and other different temperatures, the test result of different time are without obvious
Change, illustrates to preserve under proper condition, and this test strips has preferable stability.
（6）The utility model testing cost is low；To temperature without specific demand, without freezing, convenient transportation is stored, room temperature can
Preserve 24 months.
Brief description of the drawings
Fig. 1 the utility model front views（Do not draw and get stuck）；
Fig. 2 is Fig. 1 left views；
Fig. 3 the utility model gets stuck structural representation；
It is positive findings schematic diagram in Fig. 4；
It is negative findings schematic diagram in Fig. 5；
It is test strips failure schematic diagram in Fig. 6.
In figure：1 sample pad, 2 gold standard pads, 3NC films, 4 detection line T lines, 5 nature controlling line C lines, 6 adsorptive pads, 7PVC base plates, 8 cards
Shell, 9 wells, 10 observation grooves.
Specific embodiment
With reference to embodiment, the utility model is described in further detail, but implementation method of the present utility model
Not limited to this, it is impossible to protection domain of the present utility model is limited with following embodiments.
Experimental technique used in following embodiments is conventional method unless otherwise specified；The material that is used,
Reagent etc., unless otherwise specified, commercially obtains.
Embodiment 1
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVX rabbit polyclonal antibodies, the nature controlling line are coated with the detection line T lines 4
PVX goat anti-rabbit antibodies are coated with C lines 5, PVX colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 2
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVY is coated with the detection line T lines 4cRabbit polyclonal antibody, the Quality Control
PVY is coated with line C lines 5cGoat anti-rabbit antibody, PVY is combined with described gold standard pad 2cColloid gold label thing.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 3
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVY is coated with the detection line T lines 4oRabbit polyclonal antibody, the Quality Control
PVY is coated with line C lines 5oGoat anti-rabbit antibody, PVY is combined with described gold standard pad 2oColloid gold label thing.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 4
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVY is coated with the detection line T lines 4NTNRabbit polyclonal antibody, the Quality Control
PVY is coated with line C lines 5NTNGoat anti-rabbit antibody, PVY is combined with described gold standard pad 2NTNColloid gold label thing.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 5
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PLRV rabbit polyclonal antibodies, the Quality Control are coated with the detection line T lines 4
PLRV goat anti-rabbit antibodies are coated with line C lines 5, PLRV colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 6
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVS rabbit polyclonal antibodies, the nature controlling line are coated with the detection line T lines 4
PVS goat anti-rabbit antibodies are coated with C lines 5, PVS colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 7
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVM rabbit polyclonal antibodies, the nature controlling line are coated with the detection line T lines 4
PVM goat anti-rabbit antibodies are coated with C lines 5, PVM colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 8
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and PVA rabbit polyclonal antibodies, the nature controlling line are coated with the detection line T lines 4
PVA goat anti-rabbit antibodies are coated with C lines 5, PVA colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 9
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and TMV rabbit polyclonal antibodies, the nature controlling line are coated with the detection line T lines 4
TMV goat anti-rabbit antibodies are coated with C lines 5, TMV colloid gold label things are combined with described gold standard pad 2.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
Embodiment 10
A kind of Potyvirus Rapid detection test strip, as shown in Figure 1 and Figure 2, a kind of Potyvirus quick detection test paper
Bar, including sample pad 1, gold standard pad 2, NC films 3, detection line T lines 4, nature controlling line C line 5, adsorptive pads 6 and PVC base plates 7, the PVC
The top of base plate 7 is fitted with NC films 3, and the both upper ends thereof of NC films 3 is overlapped with gold standard pad 2 and adsorptive pads 6, the gold standard pad respectively
2 tops are overlapped with sample pad 1, and the detection line T lines 4 are positioned close on the surface of NC films 3 of sample pad 1, the nature controlling line C line
5 are positioned close on the NC films 3 of adsorptive pads 6, and tomato black ring virus rabbit polyclonal antibody is coated with the detection line T lines 4,
Tomato black ring virus goat anti-rabbit antibody is coated with the nature controlling line C line 5, tomato black ring is combined with described gold standard pad 2
Malicious colloid gold label thing.
In the present embodiment, as shown in figure 3, be additionally provided with outside Potyvirus Rapid detection test strip get stuck 8, it is described to get stuck
8 upper faces are provided with well 9 with the corresponding position of sample pad 1, and 8 upper faces that get stuck are corresponding with the NC films 3
Position is provided with observation groove 10.
In the present embodiment, the NC films 3 are 1-2mm with the length of the overlap joint of gold standard pad 2, and the NC films 3 are taken with adsorptive pads 6
The length for connecing is 2-3mm, and the gold standard pad 2 is 2-3mm with the length of the overlap joint of sample pad 1.
The utility model application method：Specifically include following steps：
(1) sample treatment：Potato leaf is taken, a certain amount of phosphate buffer is added, green juice, phosphorus is ground
The PH of phthalate buffer is 7.0-7.9；
(2) above-mentioned treated sample is added drop-wise in the sample pad of potato Rapid detection test strip, stands 1 ~ 5 point
Clock, observes detection line T lines and nature controlling line C line；
(3) result interpretation：It is the positive that detection line T lines and nature controlling line C line manifest aubergine；Detection line T lines do not manifest purple
Red, nature controlling line C line manifests aubergine, is as a result feminine gender；Detection line T lines and nature controlling line C line do not manifest aubergine or other
Phenomenon, points out test strips failure.
Claims (4)
1. a kind of Potyvirus Rapid detection test strip, including sample pad (1), gold standard pad (2), NC films (3), detection line T lines
(4), nature controlling line C line (5), adsorptive pads (6) and PVC base plates (7), the top of the PVC base plates (7) are fitted with NC films (3), described
NC films (3) both upper ends thereof is overlapped with gold standard pad respectively（2）And adsorptive pads（6）, gold standard pad (2) top is overlapped with sample pad
(1), the detection line T lines (4) are positioned close on NC films (3) surface of sample pad (1), and the nature controlling line C line (5) is set
On the NC films (3) of adsorptive pads (6), it is characterised in that：It is many Potyvirus rabbit to be coated with the detection line T lines (4)
Clonal antibody, is coated with Potyvirus goat anti-rabbit antibody on the nature controlling line C line (5), be combined with described gold standard pad (2)
Colloid gold label thing.
2. Potyvirus Rapid detection test strip according to claim 1, it is characterised in that：Potyvirus is quickly examined
It is additionally provided with outside test paper slip and is got stuck（8）, it is described to get stuck（8）Upper face position corresponding with sample pad (1) is provided with sample-adding
Hole（9）, it is described to get stuck（8）Upper face position corresponding with the NC films (3) is provided with observation groove（10）.
3. Potyvirus Rapid detection test strip according to claim 1, it is characterised in that：The NC films (3) and gold
The length of mark pad (2) overlap joint is 1-2mm, and the NC films (3) are 2-3mm, the gold standard pad with the length of adsorptive pads (6) overlap joint
(2) it is 2-3mm with the length of sample pad (1) overlap joint.
4. Potyvirus Rapid detection test strip according to claim 1, it is characterised in that：It is described to be coated on nature controlling line
Potyvirus goat anti-rabbit antibody on C lines (5), the Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) and
Be coated on the Potyvirus colloid gold label thing in gold standard pad (2), can be any one in following combination：
Combination 1：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVX goat anti-rabbit antibodies, coating
Potyvirus rabbit polyclonal antibody on detection line T lines (4) is PVX rabbit polyclonal antibodies, and the combination is in gold standard pad (2)
On Potyvirus colloid gold label thing be PVX colloid gold label things；
Combination 2：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVYcGoat anti-rabbit antibody, it is described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PVYcRabbit polyclonal antibody, the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PVYcColloid gold label thing；
Combination 3：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVYNTNGoat anti-rabbit antibody, institute
It is PVY to state the Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4)NTNRabbit polyclonal antibody, the combination exists
Potyvirus colloid gold label thing in gold standard pad (2) is PVYNTNColloid gold label thing；
Combination 4：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVYoGoat anti-rabbit antibody, it is described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PVYoRabbit polyclonal antibody, the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PVYoColloid gold label thing；
Combination 5：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PLRV goat anti-rabbit antibodies, described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PLRV rabbit polyclonal antibodies, and the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PLRV colloid gold label things；
Combination 6：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVS goat anti-rabbit antibodies, described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PVS rabbit polyclonal antibodies, and the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PVS colloid gold label things；
Combination 7：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVM goat anti-rabbit antibodies, described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PVM rabbit polyclonal antibodies, and the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PVM colloid gold label things；
Combination 8：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is PVA goat anti-rabbit antibodies, described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is PVA rabbit polyclonal antibodies, and the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is PVA colloid gold label things；
Combination 9：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is TMV goat anti-rabbit antibodies, described
The Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is TMV rabbit polyclonal antibodies, and the combination is marked in gold
Potyvirus colloid gold label thing on pad (2) is TMV colloid gold label things；
Combination 10：The Potyvirus goat anti-rabbit antibody being coated in nature controlling line C line (5) is tomato black ring virus goat-anti rabbit
Antibody, the Potyvirus rabbit polyclonal antibody being coated on detection line T lines (4) is that tomato black ring virus rabbit polyclonal resists
Body, Potyvirus colloid gold label thing of the combination in gold standard pad (2) is tomato black ring virus colloid gold label thing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621330258.9U CN206208905U (en) | 2016-12-06 | 2016-12-06 | A kind of Potyvirus Rapid detection test strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621330258.9U CN206208905U (en) | 2016-12-06 | 2016-12-06 | A kind of Potyvirus Rapid detection test strip |
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| Publication Number | Publication Date |
|---|---|
| CN206208905U true CN206208905U (en) | 2017-05-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621330258.9U Active CN206208905U (en) | 2016-12-06 | 2016-12-06 | A kind of Potyvirus Rapid detection test strip |
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| CN (1) | CN206208905U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109752545A (en) * | 2018-12-11 | 2019-05-14 | 苏州农业职业技术学院 | A kind of viral diagnosis equipment of potato |
| CN111650374A (en) * | 2019-12-31 | 2020-09-11 | 贵州省烟草科学研究院 | Rapid test card for simultaneously detecting TMV and TVBMV and preparation and use methods thereof |
-
2016
- 2016-12-06 CN CN201621330258.9U patent/CN206208905U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109752545A (en) * | 2018-12-11 | 2019-05-14 | 苏州农业职业技术学院 | A kind of viral diagnosis equipment of potato |
| CN111650374A (en) * | 2019-12-31 | 2020-09-11 | 贵州省烟草科学研究院 | Rapid test card for simultaneously detecting TMV and TVBMV and preparation and use methods thereof |
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