CN206196779U - A kind of cell glass freezing processing equipment - Google Patents

A kind of cell glass freezing processing equipment Download PDF

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Publication number
CN206196779U
CN206196779U CN201621122095.5U CN201621122095U CN206196779U CN 206196779 U CN206196779 U CN 206196779U CN 201621122095 U CN201621122095 U CN 201621122095U CN 206196779 U CN206196779 U CN 206196779U
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CN
China
Prior art keywords
straw
drive division
cell
freezing
drive
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CN201621122095.5U
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Chinese (zh)
Inventor
林小贞
伽博·瓦吉它
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Shenzhen Wei Wei Biotechnology Co Ltd
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Shenzhen Wei Wei Biotechnology Co Ltd
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Priority to CN201621122095.5U priority Critical patent/CN206196779U/en
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Abstract

The utility model discloses a kind of cell glass freezing processing equipment, including straw device, precooling device, freezing unit, drive device, control unit, and for carrying the plummer of cell carrier, the straw device and the drive device drive connection, the drive device is connected with described control unit signal, the straw device is used to obtain the cell in the cell carrier, described control unit is used to control the drive device to drive the straw device to obtain cell and the cell is transferred into the freezing unit, the precooling device carries out precooling during the cell of acquisition is transferred to the freezing unit by the straw device to the straw.Processing equipment of the present utility model realizes full-automatic operation, reduces manual operation error of the prior art, is easy to batched operation, while without operating personnel's close contact liquid nitrogen, improve security.

Description

A kind of cell glass freezing processing equipment
Technical field
The utility model is related to cell glass freezing processing technology field, in particular it relates to a kind of cell vitrifying is cold Freeze processing equipment.
Background technology
With the need of the aspect such as the development of biotechnology and biomedicine, human ancillary reproductive and animal husbandry conservation breeding Ask.Cell glass freezing treatment technology by the development of more than 60 years, into a truly feasible cell and biological tissue Store method.The freezen protective of wherein human egg is of greatest concern, and application prospect is most wide.China infertile rate by 3% in 1992 improves about 20% till now, and it is test-tube baby to solve infertile major medical approach.China 2009 Have 138 Jia Shi test-tube babies mechanisms year, the cut-off whole nation in 2012 has 356 approvals and carries out Issues of Human Assisted Reproductive Technologies machine Structure, 17 sperm banks.Although short 3 years, mechanism's quantity increases by 1 times, and line city test-tube baby operation need to averagely queue up partly More than year, in serious supply falls short of demand state.It is that current cell vitrifying is cold to cause the one of main cause of this state Freeze treatment technology efficiency low.Current cell vitrifying treatment technology flow by manually operating, the qualification of operating personnel's technology The stability worked with regular job directly affects the anabiosis rate after cell freezing.Because this operates the degree that becomes more meticulous very high, So one work of qualified operating personnel of training is time-consuming extremely long, the Material Cost of consumption is very high, so artificial operation is not only Waste time and energy, and efficiency is low.Further, since cell glass freezing is mainly by liquid nitrogen to freeze, and carry out with liquid for a long time The relevant artificial operation of nitrogen also has certain risk.
Utility model content
The technical problems to be solved in the utility model be to provide it is a kind of being capable of automation mechanized operation and treatment effeciency cell high Glass freezing processing equipment.
In order to solve the above-mentioned technical problem, the utility model provides a kind of cell glass freezing processing equipment, described Processing equipment includes straw device, precooling device, freezing unit, drive device, control unit and is carried for carrying cell The plummer of body, the straw device and the drive device drive connection, the drive device and described control unit signal Connection, the straw device includes the straw for obtaining the cell in the cell carrier, and described control unit is used to control The drive device drives the straw device to obtain cell and the cell is transferred into the freezing unit, the precooling Device provides freezing and is situated between during the cell of acquisition is transferred to the freezing unit by the straw device to the straw Matter carries out precooling with to the cell.
Further, the drive device includes the first drive division and the second drive division, and first drive division is along first Direction is moved back and forth, and second drive division is moved back and forth in a second direction, and the first direction and second direction are mutually perpendicular to, The plummer and the freezing unit are fixedly connected with first drive division, and the straw device drives with described second Dynamic portion is fixedly connected.
Further, the drive device also includes the 3rd drive division, and the 3rd drive division is back and forth transported along third direction Dynamic, the 3rd drive division is slidably connected with first drive division or the second drive division, and the third direction is perpendicular to described First direction and second direction.
Further, the straw device includes the straw and the straw for fixing the straw for drawing cell Fixture.
Further, the straw fixture includes fairlead, push rod and the straw for drawing cell, the straw position In in the fairlead and can be along the axial movement of the fairlead, the push rod be located in the fairlead and can be along institute State the axial movement of fairlead;The push rod is located at the top of the straw, so that the push rod in predefined conditions will be described Straw ejects the fairlead.
Further, the processing equipment also includes the position detecting device for detecting the straw position, institute's rheme Put detection means to be connected with described control unit signal, for the positional information of the straw to be transferred into described control unit.
Further, the straw fixture also includes installation portion, and the installation portion is fixedly connected with the fairlead, described Installation portion is fixedly connected with second drive division, and the straw device is fixed by the installation portion with second drive division Connection.
Further, the straw device also includes the drive division for driving the push rod to move reciprocatingly, the drive division It is fixedly connected with the push rod, and the drive division is connected with described control unit signal.
Further, the quantity of the straw is multiple, and the quantity of the push rod is multiple, the quantity of the straw and institute The quantity for stating push rod is identical, and the drive division is in tabular, and the drive division is fixedly connected with the top of multiple push rods.
Further, the straw fixture also includes installation portion, and the installation portion is fixedly connected with the fairlead, described Installation portion is fixedly connected with second drive division, and the straw device is fixed by the installation portion with second drive division Connection.
Further, the processing equipment also includes mounting bracket, and the mounting bracket includes guide post, the fixed part The slideway coordinated with the guide post is provided with, the fixed part is slidably connected with the guide post, second drive division drives The fixed part is moved to be moved up and down along the guide post.
Further, the precooling device include liquid nitrogen memory cell, liquid nitrogen gasification unit, pumping unit, nozzle with And pipeline, the liquid nitrogen gasification unit includes gasification chamber, and the pumping unit is connected with by institute with the liquid nitrogen memory cell The liquid nitrogen stated in liquid nitrogen memory cell is pumped to the gasification chamber through the pipeline, and the nozzle connects with the gasification chamber It is logical;Described control unit is connected with the pumping unit signal, startup and stopping for controlling the pumping unit, the wheat Pipe device includes the straw for drawing the cell carrier, and the liquid nitrogen after gasifying through the liquid nitrogen gasification unit is by the spray Mouth sprays to the straw of the straw device.
Further, the precooling device also includes the pipe being arranged between the pumping unit and the gasification chamber Switch valve on road, described control unit is connected with the switch valve with signal, unlatching and pass for controlling the switch valve Close.
Further, the liquid nitrogen gasification unit also includes fixed part, and the inner hollow of the fixed part forms the gas Change chamber, the fixed part includes inlet and gas outlet, and the gasification chamber is described by inlet and the pipeline connection Nozzle is fixedly connected on the gas outlet of the fixed part, and is connected with the gasification chamber by the gas outlet, the fixation Portion is fixedly connected with second drive division.
Further, the precooling device also includes heating part, and the heating part is surrounded on the outer nozzle wall, described Heater is connected with described control unit signal.
Further, the precooling device includes for containing the refrigerated container of refrigerant, the refrigerated container with First drive division is fixedly connected.
Further, the freezing unit includes freezing tank and the sleeve pipe for accommodating the straw, and described sleeve pipe sets It is placed in the freezing tank, refrigerant is contained in the freezing tank, the freezing tank is fixedly connected with first drive division.
The quantity of described sleeve pipe is multiple, multiple described sleeve pipe forming sleeves arrays, and multiple described sleeve pipes by support It is fixed in the freezing tank.
Further, the freezing unit also includes the slip lid for covering the freezing tank, and the slip lid is arranged at institute Freezing tank top is stated, and the slip lid can be along the top slide of the freezing tank.
Processing equipment of the present utility model can control the drive device to drive the straw device certainly by control unit Dynamic acquisition cell and the automatic cell of acquisition is transferred in the freezing unit are freezed, during transfer, also Precooling is carried out by controlling the precooling device to spray refrigerant automatically to the straw, full-automatic behaviour is realized Make, reduce manual operation error of the prior art, be easy to batched operation, treatment effeciency to be greatly improved, together Shi Wuxu operating personnel's close contact refrigerant, improves security.
Brief description of the drawings
Fig. 1 is the block schematic illustration of the cell glass freezing processing equipment of the embodiment of the utility model one;
Fig. 2 is the structural representation of the cell glass freezing processing equipment of the embodiment of the utility model one;
Fig. 3 is the straw apparatus structure schematic diagram of the embodiment of the utility model one;
Fig. 4 is the precooling apparatus structure schematic diagram of the embodiment of the utility model one;
Fig. 5 is the sectional view along the A-A directions in Fig. 2;
Fig. 6 is the schematic diagram of the cell suction process in the embodiment of the utility model one;
The schematic diagram of the cell precooling process in the embodiment of Fig. 7 the utility model one;
The schematic diagram of cell transfer and sleeve pipe process in the embodiment of Fig. 8 the utility model one;
Fig. 9 is the structural representation of the cell glass freezing processing equipment of another embodiment of the utility model;Wherein, it is right The structure of the straw device is simplified.
Main element symbol description:
12nd, the first drive division 14, the second drive division 22, straw 24, push rod
26th, fairlead 28, installation portion 31, nozzle 35, pipeline
32nd, liquid nitrogen memory cell 33, liquid nitrogen gasification unit 332, gasification chamber 334, fixed part
36th, switch valve 37, heating part 40, control unit 52, plummer
54th, mounting bracket 542, guide post 56, supporting table 62, freezing tank
64th, sleeve pipe 66, slip lid 70, cell carrier 23, drive division
30th, refrigerated container
Following specific embodiment will further illustrate the utility model with reference to above-mentioned accompanying drawing.
Specific embodiment
The utility model is described in further detail with specific embodiment below in conjunction with the accompanying drawings, so that those skilled in the art The utility model may be better understood and can be practiced, but illustrated embodiment is not as to restriction of the present utility model.
The utility model embodiment provides a kind of cell glass freezing processing equipment, refers to Fig. 1 and Fig. 2, the place Reason equipment includes straw device, precooling device, freezing unit, drive device, control unit 40 and is carried for carrying cell The plummer 52 of body 70, the straw device and the drive device drive connection, the drive device and described control unit 40 signals are connected, and the straw device includes the straw 22 for obtaining the cell in the cell carrier 70, and the control is single Unit 40 is single for controlling the drive device driving straw device acquisition cell and the cell being transferred into the freezing Unit, the precooling device is during the cell of acquisition is transferred to the freezing unit by the straw device to the wheat Pipe provides refrigerant and carries out precooling with to the cell.Processing equipment of the present utility model can be controlled by control unit 40 The drive device drives that the straw device obtains cell automatically and automatic that the cell of acquisition is transferred into the freezing is single Freezed in unit, during transfer, the precooling device automatically provides refrigerant and carries out to the straw device Precooling, realizes full-automatic operation, reduces manual operation error of the prior art, is easy to batched operation so that Glass freezing treatment effeciency is greatly improved, while without operating personnel's close contact refrigerant, improve Security.
The cell carrier 70 can be but not limited to slide, and the drop containing cell is dropped on wave carrier piece, in institute Before stating processing equipment use, by slide side on the plummer 52.
The straw device includes straw 22 and straw fixture, and the straw 22 is used to draw cell, the straw folder Have for fixing the straw 22.The mode that the straw fixture fixes the straw 22 can be clamping, Electrostatic Absorption, use gas Pressure difference holds, is fixed with magnetic field or electromagnetic field effect.It is (as thin in drawn cell and transfer when the straw 22 needs to be fixed During born of the same parents), the straw fixture fixes the straw 22;(processed as described in cell enters when the straw 22 needs to be released After the sleeve pipe of equipment), the straw fixture discharges the straw 22.
Further, Fig. 3 is referred to, in first embodiment of the present utility model, the straw fixture includes fairlead 26 With push rod 24, the fairlead 26 is used to position the straw 22 and the motion to the straw 22 play the guiding role, Specifically, the straw 22 is located in the fairlead 26 and can be along axial direction (the i.e. length of fairlead 26 of the fairlead 26 Degree direction) move, the push rod 24 is located in the fairlead 26 and can be along the axial movement of the fairlead 26;The top Bar 24 is located at the top of the straw 22, so that the straw 22 is ejected the fairlead by the push rod 24 in predefined conditions 26.For example, when the needs straw 22 is put into (this i.e. described predetermined condition) described push rod when the freezing unit is freezed 24 are moved downward, and the straw 22 is ejected into the fairlead 26.The straw 22 coordinates with the gap of the fairlead 26, thus By the appropriate frictional force between the straw 22 and the inwall of the fairlead 26 so that the straw 22 not need other Required position is positively retained in the case of fixing device.The straw 22 can be flexible pipe, can so be easy to the flexible pipe Snap in the fairlead 26, and the fairlead 26 will not be caused to damage, certainly, the straw 22 may also be hard tube.Enter One step ground, the straw fixture also includes installation portion 28, and the installation portion 28 is fixedly connected with the fairlead 26, the installation Portion 28 is also fixedly connected with the drive device simultaneously, and the straw device is solid with the drive device by the installation portion 28 Fixed connection, specifically, the installation portion 28 has mounting hole, and the fairlead 26 is fixed and is embedded in the mounting hole.It is described The shape of installation portion 28 is not limited, for example, referring to Fig. 2, the installation portion 28 can be but not limited in tabular.
The quantity of the straw 22 is not limited, and can be one, or multiple, the multiple composition of the straws 22 straw battle arrays Row, can simultaneously obtain the cell in multiple cell carriers 70, further improve the treatment effeciency of processing equipment.
The mode that the straw 22 draws cell is not limited, for example, can be but not limited to by capillary force or use draught head Mode is drawn.
For the ease of driving the push rod 24, the straw device also includes drive division 23, the drive division 23 with it is described Push rod 24 is fixedly connected, and the drive division 23 is connected with described control unit signal, for driving the push rod 24 along described The axially reciprocating of fairlead 26, it is necessary to explanation, the drive division 23 can be with by oil cylinder, cylinder or other Electrical equipment is driving source, the not shown driving sources of Fig. 2.The straw 22 is one-to-one corresponding with the push rod 24 and fairlead 26 Relation, in order to further lift the treatment effeciency of the straw device, the quantity of the straw 22 is multiple, the push rod 24 Multiple is with the quantity of the fairlead 26, and is corresponded with the quantity of the straw 22, multiple straws 22 form straw Array, can make straw device disposably draw various kinds of cell or a large amount of cells, greatly improve treatment effeciency.Further, it is described Drive division 23 is fixedly connected in tabular, and the drive division 23 with the top of multiple push rods 24, by the drive division 23 Elevating movement can drive the synchronization lifting of multiple push rods 24 to move.
Please continue to refer to Fig. 2, the drive device includes the first drive division 12 and the second drive division 14, and described first drives Portion 12 moves back and forth (left and right directions of Fig. 2) in the first direction, and in the present embodiment, the first direction is horizontal direction, described Second drive division 14 moves back and forth (above-below direction of Fig. 2) in a second direction, and in the present embodiment, the second direction is square vertically To and the first direction and second direction be mutually perpendicular to, and the plummer 52 and the freezing unit are with described first Drive division 12 is fixedly connected, and the plummer 52 and the freezing unit are under the drive of first drive division 12 with described The level of synchronization of one drive division 12 is moved, and the straw device is fixed with second drive division 14 by the installation portion 28 and connected Connect, the straw device follows second drive division 14 synchronous vertically movable under the drive of second drive division 14.
It should be noted that first drive division 12 refers to can drive the plummer 52 and freezing unit in first party Upward reciprocating driver part, therefore, first drive division 12 is not to refer in particular to a single driver part, may be used yet Being the general name of multiple driver parts.For example in a first direction, can be that the plummer 52 is connected with a driver part, The freezing unit is connected with another driver part, now, drives the driver part of the plummer 52 described cold with driving The driver part for freezing unit is referred to as the first drive division 12.
Fig. 2 is referred to, the freezing unit includes freezing tank 62 and the sleeve pipe 64 for accommodating the straw 22, described Sleeve pipe 64 is arranged in the freezing tank 62.Described sleeve pipe 64 can be fixed in the freezing tank 62 by support, be should be noted That " fixation " herein means that described sleeve pipe can not be moved relative to the freezing tank, described sleeve pipe 64 need when Waiting can take out it from the freezing tank 62.The internal memory of the freezing tank 62 is placed with refrigerant, the refrigerant class Type is not limited, as long as it can provide ultra-low temperature surroundings (being equal to or less than -196 degrees Celsius).In the present embodiment, the freezing is situated between Matter is liquid nitrogen.The quantity of described sleeve pipe 64 can be set according to actual needs, for example, can set multiple sleeve pipes 64 in sleeve pipe Array arrangement, so can further improve the treating capacity of equipment, and then lift treatment effeciency.Further, the freezing unit is also Including slip lid 66, the slip lid 66 is arranged at the top of the freezing tank 62, and the slip lid 66 can be along the top of the freezing tank 62 Slide in portion.
When the processing equipment of the present embodiment is operated, cell carrier 70 is positioned on the plummer 52 first, described the Two drive divisions 14 drive the straw device that the default position of the top of cell carrier 70 is moved to second direction (lower section of Fig. 2) Put, the predeterminated position can cause that the straw 22 just siphons away the cell in cell carrier 70, second drive division 14 The straw device is driven to move up to certain altitude, in first scheduled time after the straw 22 siphons away cell, institute State precooling device carries out precooling to the offer refrigerant of the straw 22 with to the cell, then first drive division 12 in the first direction (left of Fig. 2) motion to drive the freezing unit to move to the underface of the straw device, it is described Second drive division 14 drives the straw device to move downwardly at freezing unit, and the drive division 23 drives the push rod 24 to incite somebody to action The straw 22 ejects the fairlead 26, and the straw 22 heads into the sleeve pipe 64 of the freezing unit.Treat the set Be packaged for the taking-up of described sleeve pipe 64 after retaining for second scheduled time in the freezing tank 62 by pipe 64, the set that will be encapsulated afterwards Pipe 64 is put into such as liquid nitrogen container refrigerated source and carries out long-term frozen preservation.
It is accurately positioned for the ease of the run location up and down to the straw device, the processing equipment also includes position Detection means (not shown) is put, the position detecting device is connected with the signal of described control unit 40, by the straw device Positional information be transferred to described control unit 40.The installation site of the position detecting device is not limited, as long as it can smoothly be examined Measure the position of the straw device.The type of the position detecting device is not limited, for example, can be, but not limited to light establishing by cable Close.When the precision of the optoelectronic switch is at least in 0.05mm, positioning accuracy request can be met.
It is oriented to for the ease of the motion to the straw device, the processing equipment also includes mounting bracket 54, institute Stating mounting bracket 54 includes guide post 542, and the slideway coordinated with the guide post 542 is provided with the installation portion 28.It is described Installation portion 28 is slidably connected with the guide post 542, and second drive division 14 drives the installation portion 28 along the guide post 542 move up and down.In the present embodiment, the slideway is the through hole for being arranged at the installation portion 28, and the guide post 542 wears In the through hole.Further, the guide post is multiple, and multiple guide posts are distributed in the side of the installation portion 28 Edge, can so obtain more preferable guide effect.
Certainly, the processing equipment also includes supporting table 56, and the drive division 14 of first drive division 12 and second is slided It is installed on dynamicly in the supporting table 56, the mounting bracket 54 is fixedly installed in the supporting table 56.
Further, the drive device also includes the 3rd drive division (not shown), and the 3rd drive division is along third party Moved back and forth to (perpendicular to the direction of paper in Fig. 2), the 3rd drive division drives with first drive division 12 or second Portion 14 is slidably connected, and the third direction is perpendicular to the first direction and second direction.First drive division 12, second drives The routing motion of the dynamic drive division of portion 14 and the 3rd, can meet the straw device and be filled with the cell carrier 70, the straw Put and the position alignment demand between the freezing unit.
It should be noted that first drive division 12, the second drive division 14 and the 3rd drive division can be automatically controlled drives It is dynamic, it is also possible to which that hydraulic-driven or gas control drive.
Fig. 4 and Fig. 5 is referred to, in first embodiment of the present utility model, it is single that the precooling device includes that liquid nitrogen is stored Unit 32, liquid nitrogen gasification unit 33, pumping unit, nozzle 31, pipeline 35, the liquid nitrogen gasification unit 33 include gasification chamber 332, The pumping unit be connected with the liquid nitrogen memory cell 32 with by the liquid nitrogen in the liquid nitrogen memory cell 32 through the pipeline 35 are pumped to the gasification chamber 332, and the nozzle 31 is connected with the gasification chamber 332, described control unit and the pump Cell signal is sent to connect, startup and stopping for controlling the pumping unit;The straw device is included for drawing cell The straw of carrier, the liquid nitrogen after gasifying through the liquid nitrogen gasification unit sprays to the straw by the nozzle.The control is single Unit 40 and the precooling device also include being arranged on the pipeline 35 between the pumping unit and the gasification chamber 332 Switch valve 36, described control unit 40 is connected with the switch valve 36 with signal, the opening and closing for controlling the switch valve 36.When Described control unit 40 is detected after the pumping unit pumps out liquid nitrogen to certain pressure, controls the switch valve 36 to open, and is made Obtain liquid nitrogen to gasify because of heating up in the gasification chamber 332, and sprayed by nozzle 31, precooling is carried out to cell.
The type of the switch valve 36 is not limited, but because the temperature of liquid nitrogen is very low, the switch valve 36 needs that low temperature can be born, And at low temperature can normal work, for example, the switch valve 36 can be but not limited to low-temperature solenoid valve, the low-temperature solenoid valve Electrically connected with described control unit 40, when the liquid nitrogen pressure in the pipeline 35 reaches a preset value, described control unit 40 Control the low-temperature solenoid valve obtain it is electric after open, the liquid nitrogen in the pipeline 35 enters after low-temperature solenoid valve, part pipeline 35 The gasification chamber 332.It is of course also possible to be, after described control unit 40 controls the pumping unit pumping scheduled time, control Make the low-temperature solenoid valve to open, i.e., the described low-temperature solenoid valve delayed pumping unit scheduled time opens.The utility model Can be by regulating and controlling the pressure and low-temperature solenoid valve folding time and then speed, temperature to control the ejection of liquid nitrogen of pumping unit Degree and time.
The pumping unit is that structure and type are not limited, as long as liquid nitrogen can be pumped out, for example, in the present embodiment, institute Pumping unit is stated for booster.
The liquid nitrogen gasification unit 33 also includes fixed part 334, and the fixed part 334 is used to form the gasification chamber 332, specifically, refer to Fig. 4, the inner hollow of the fixed part 334 is forming the gasification chamber 332, the fixed part The first through hole is provided with 334 as inlet and the second through hole is provided with as gas outlet, the gasification chamber 332 Connected with the inlet, and connected with the pipeline 35 by the inlet, the gasification chamber 332 simultaneously also with it is described Gas outlet connects, and the nozzle 31 is fixedly connected with the fixed part 334, and is connected with the gas outlet;The gasification chamber 332 are connected by the gas outlet with the nozzle 31, and the gasification nitrogen jet in the gasification chamber 332 is gone out.Enter One step ground, the fixed part 334 is fixedly connected with second drive division 14, in this way, driving institute in second drive division 14 The fixed part 334 and nozzle 31 can be synchronously driven to follow it to be synchronized with the movement when stating straw device vertical motion, both this ensures institute State being synchronized with the movement for nozzle 31 and the straw device so that the relative position between nozzle 31 and the straw 22 is constant, with Ensure the accuracy in injection orientation, improve the processing accuracy and efficiency of equipment.According to actually used occasion, the gas outlet is located at The top of the fixed part 334, the gas outlet is located at the bottom of the fixed part 334, in order to the behaviour to cell precooling Make.
In order to prevent the temperature of the nozzle 31 extremely low so that causing the nozzle 31 to block into the hydrogenesis in environment, The injection apparatus also includes heating part 37, and the heating part 37 is surrounded on the outer wall of the nozzle 31, the heater with it is described Control unit 40 is electrically connected, with will pass through described control unit 40 control the heater it is in good time to the nozzle 31 heat with Anti-clogging.
In order to take into account the rational in infrastructure of precooling effect and equipment, the quantity of the precooling device is two, described The opposite sides side of straw device connects a precooling device respectively, two side can be front and rear both sides or The left and right sides, is the left and right sides of straw device in the present embodiment, under straw described in the nozzle alignment of the precooling device End, it is integral type structure that the fixed part can be with the installation portion, or both be Split type structure by being fixedly connected on Together, in this way, may be such that the relative position between the nozzle and the straw is constant, therefore only need to installation portion or solid Determine portion to be fixedly connected with second drive division, you can drive the straw device and the precooling device with described second Portion is fixedly connected, and second drive division can drive the straw device and the precooling device to be synchronized with the movement.
The workflow of the processing equipment of the utility model first embodiment is as follows:
The cell carrier 70 (such as chip) that cell will be loaded with is positioned on the plummer 52, certainly, for modular working Flow, the fixed screens with the array of liquid cell carrier 70, fixed screens is fixed on by cell carrier 70 on the plummer 52 On, after straw 22 and sleeve pipe 64 are installed by hand, equipment is started working, and operating process is as follows:
1. cell suction process, refers to Fig. 6 (in the case of shown in Fig. 6, the straw 22 will draw cell):The control Unit processed 40 controls first drive division 12 to drive the precalculated position of plummer 52 to the first, and first precalculated position is by institute State control unit 40 to preset, described control unit 40 controls the second drive division 14 to drive the straw device to move downwardly to Second precalculated position, second precalculated position is preset by described control unit 40, and anti-in real time by position detecting device Feedback so that the straw 22 can just pick up drop (containing cell);
2. cell precooling process, (in the case of shown in Fig. 7, the precooling device is to the straw 22 to refer to Fig. 7 Injection liquid nitrogen gas):Described control unit 40 controls second drive division 14 to drive the straw device to move upwards, works as liquid Drop picked up first it is predetermined between interior (in such as 10 seconds), described control unit 40 controls the precooling injection dress to straw 22 Spray the liquid nitrogen gas several seconds in lower end;First scheduled time is preset by described control unit 40.
3. cell is shifted and sleeve pipe process, and (in the case of shown in Fig. 8, described sleeve pipe 22 has been placed in the set to refer to Fig. 8 In pipe 64):It is predetermined that described control unit 40 controls first drive division 12 to drive the freezing unit to move to the 3rd to the left Position, the slip lid 66 of the freezing tank 62 is opened, and second drive division 14 drives the straw device to decline so that the wheat Pipe 22 is directed at described sleeve pipe 64, and the drive division 23 drives the push rod 24 that the straw 22 is ejected into the fairlead 26 And in heading into described sleeve pipe 64, the straw device (in addition to straw 22) is up moved.
4. the first drive division 12 is moved right described in, after the straw 22 enters the scheduled time of described sleeve pipe 64 (such as 60 seconds) 64 groups of sleeve pipe is taken out afterwards is packaged (this process preserve for sleeve pipe encapsulation), in placing into liquid nitrogen container or other refrigerated sources Long-term frozen is preserved.
Fig. 9 is referred to, the precooling device of second embodiment of the present utility model is included for containing refrigerant Refrigerated container 30, the refrigerated container 30 is fixedly connected with second drive division.When the straw device draw cell with Afterwards, first drive division 12 drives the refrigerated container 30 to move to the lower section of the straw 22, second drive division 14 Drive the straw device to move downward and be immersed in the refrigerated container 30 with by the end equipped with cell in the straw 22 Refrigerant in precooling is carried out to cell, after precooling Preset Time, second drive division 14 drives the straw to fill Motion upwards is put to be taken out from the refrigerated container 30 with by the straw 22.Certainly, it is described for further simplified structure Refrigerated container 30 can be same with the freezing tank, share same refrigerant, such as liquid nitrogen.
Processing equipment of the present utility model realizes full-automatic operation, can replace it is artificial realize glass freezing during Cell is drawn, cell is shifted, the treatment of cell precooling and sleeve pipe preserve high efficiency, automation, the high flux treatment closed.Simultaneously Also contribute to set up standardized handling process, improve and stabilization cell freezing recovery survival rate, meet growing huge Industry demand.
Embodiment described above is only the preferred embodiment lifted to absolutely prove the utility model, the utility model Protection domain not limited to this.Equivalent substitute or change that those skilled in the art are made on the basis of the utility model Change, within protection domain of the present utility model.Protection domain of the present utility model is defined by claims.

Claims (18)

1. a kind of cell glass freezing processing equipment, it is characterised in that the processing equipment includes that straw device, precooling are filled Put, freezing unit, drive device, control unit and the plummer for carrying cell carrier, the straw device with it is described Drive device drive connection, the drive device is connected with described control unit signal, and the straw device is included for obtaining The straw of the cell in the cell carrier, described control unit is used to control the drive device to drive the straw device to obtain Take cell and the cell is transferred to the freezing unit, the cell that the precooling device will be obtained in the straw device There is provided refrigerant to the straw device during being transferred to the freezing unit carries out precooling with to the cell.
2. processing equipment as claimed in claim 1, it is characterised in that the drive device includes that the first drive division and second drives Dynamic portion, first drive division is moved back and forth in the first direction, and second drive division is moved back and forth in a second direction, and described the One direction and second direction are mutually perpendicular to, and the plummer and the freezing unit are fixed with first drive division and connected Connect, the straw device is fixedly connected with second drive division.
3. processing equipment as claimed in claim 2, it is characterised in that the drive device also includes the 3rd drive division, described 3rd drive division is moved back and forth along third direction, and the 3rd drive division is slided with first drive division or the second drive division and connected Connect, the third direction is perpendicular to the first direction and second direction.
4. processing equipment as claimed in claim 2, it is characterised in that the straw device includes the straw for drawing cell And for fixing the straw fixture of the straw.
5. processing equipment as claimed in claim 4, it is characterised in that the straw fixture includes fairlead and push rod, described Straw is located in the fairlead and can be along the axial movement of the fairlead, and the push rod is located in the fairlead and can Along the axial movement of the fairlead;The push rod is located at the top of the straw, so as to the push rod in predefined conditions The straw is ejected into the fairlead.
6. processing equipment as claimed in claim 4, it is characterised in that the processing equipment is also included for detecting the straw The position detecting device of position, the position detecting device is connected with described control unit signal, for by the position of the straw Confidence breath is transferred to described control unit.
7. processing equipment as claimed in claim 5, it is characterised in that the straw device also includes driving the push rod to do past The drive division of multiple motion, the drive division is fixedly connected with the push rod, and the drive division connects with described control unit signal Connect.
8. processing equipment as claimed in claim 7, it is characterised in that the quantity of the straw is multiple, the number of the push rod It is multiple to measure, and the quantity of the straw is identical with the quantity of the push rod, the drive division be in tabular, the drive division with it is many The top of the individual push rod is fixedly connected.
9. processing equipment as claimed in claim 5, it is characterised in that the straw fixture also includes installation portion, the installation Portion is fixedly connected with the fairlead, and the installation portion is fixedly connected with second drive division, and the straw device passes through institute Installation portion is stated to be fixedly connected with second drive division.
10. processing equipment as claimed in claim 9, it is characterised in that the processing equipment also includes mounting bracket, the peace Dress support includes guide post, and the fixed part is provided with the slideway coordinated with the guide post, the fixed part and the guiding Bar is slidably connected, and second drive division drives the fixed part to be moved up and down along the guide post.
11. processing equipments as claimed in claim 2, it is characterised in that the precooling device includes liquid nitrogen memory cell, liquid Nitrogen gasification unit, pumping unit, nozzle and pipeline, the liquid nitrogen gasification unit include gasification chamber, the pumping unit with The liquid nitrogen memory cell connection is pumped to the gasification chamber with by the liquid nitrogen in the liquid nitrogen memory cell through the pipeline, The nozzle is connected with the gasification chamber;Described control unit is connected with the pumping unit signal, for controlling the pump Send the startup and stopping of unit;The straw device includes the straw for drawing cell carrier, through the liquid nitrogen gasification unit Liquid nitrogen after gasification sprays to the straw by the nozzle.
12. processing equipments as claimed in claim 11, it is characterised in that the precooling device also includes being arranged at the pump The switch valve on the pipeline between unit and the gasification chamber is sent, described control unit is connected with the switch valve with signal, Opening and closing for controlling the switch valve.
13. processing equipments as claimed in claim 11, it is characterised in that the liquid nitrogen gasification unit also includes fixed part, institute The inner hollow for stating fixed part forms the gasification chamber, and the fixed part includes inlet and gas outlet, the gasification chamber By inlet and the pipeline connection, the nozzle is fixedly connected on the gas outlet of the fixed part, and by the outlet Mouth is connected with the gasification chamber, and the fixed part is fixedly connected with second drive division.
14. processing equipments as claimed in claim 11, it is characterised in that the precooling device also includes heating part, described Heating part is surrounded on the outer nozzle wall, and the heater is connected with described control unit signal.
15. processing equipments as claimed in claim 2, it is characterised in that the precooling device includes being situated between for containing freezing The refrigerated container of matter, the refrigerated container is fixedly connected with first drive division.
16. processing equipments as claimed in claim 2, it is characterised in that the freezing unit includes freezing tank and for holding Receive the sleeve pipe of the straw, described sleeve pipe is arranged in the freezing tank, and refrigerant, the freezing are contained in the freezing tank Groove is fixedly connected with first drive division.
17. processing equipments as claimed in claim 16, it is characterised in that the quantity of described sleeve pipe is multiple, multiple sets Pipe forming sleeves array, and multiple described sleeve pipe is fixed in the freezing tank by support.
18. processing equipments as claimed in claim 16, it is characterised in that the freezing unit also includes described cold for covering Freeze the slip lid of groove, the slip lid is arranged at the freezing tank top, and the slip lid can be along the top slide of the freezing tank.
CN201621122095.5U 2016-10-13 2016-10-13 A kind of cell glass freezing processing equipment Active CN206196779U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109964924A (en) * 2018-05-31 2019-07-05 北京臻溪谷医学研究中心(有限合伙) Automatic operation system, bogey for cell glass freezing
CN113812394A (en) * 2021-09-06 2021-12-21 刘荣怀 Mesenchymal stem cell storage box

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109964924A (en) * 2018-05-31 2019-07-05 北京臻溪谷医学研究中心(有限合伙) Automatic operation system, bogey for cell glass freezing
CN109964924B (en) * 2018-05-31 2021-11-26 北京臻溪谷医学研究中心(有限合伙) Automatic operation equipment and carrying device for cell vitrification freezing
CN113812394A (en) * 2021-09-06 2021-12-21 刘荣怀 Mesenchymal stem cell storage box

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Inventor after: Lin Xiaozhen

Inventor after: Gai Bo.wajita

Inventor before: Lin Xiaozhen

Inventor before: Gabor Vagita

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Address after: 518000 Room 601, building B, haikexing strategic emerging industrial park, No. 16, Baoshan Road, Pingshan street, Pingshan District, Shenzhen, Guangdong

Patentee after: SHENZHEN VITAVITRO BIOTECH Co.,Ltd.

Address before: Room 501, building B, Haike new strategic emerging industrial park, No.16 Baoshan Road, Pingshan street, Pingshan New District, Shenzhen City, Guangdong Province

Patentee before: SHENZHEN VITAVITRO BIOTECH Co.,Ltd.