CN206132772U - Reflective optical detection device of intelligence expiration molecular diagnosis system - Google Patents
Reflective optical detection device of intelligence expiration molecular diagnosis system Download PDFInfo
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- CN206132772U CN206132772U CN201621206304.4U CN201621206304U CN206132772U CN 206132772 U CN206132772 U CN 206132772U CN 201621206304 U CN201621206304 U CN 201621206304U CN 206132772 U CN206132772 U CN 206132772U
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Abstract
The utility model relates to a detection device of expiration molecular diagnosis system. Reflective optical detection device of intelligence expiration molecular diagnosis system includes reaction tank, biosensor and is used for spectral analysis's light path structure, and biosensor is including being provided with the reaction chamber with the biomarker reaction of human body exhalation and with the absorptive reactant of biomarker in the reaction tank, and biosensor's reactant is encapsulated in the reaction chamber, and reaction tank and biosensor are located in the beam propagation route of light path structure, the light path structure can produce the light beam and jet into the interior and process biosensor of reaction chamber, and biosensor jets out the reaction tank after can making the light beam pass the reactant light beam multiple reflection many times, and the light path structure can obtain the absorption spectrum of reactant through the light beam that jets out the reaction tank. The utility model discloses effectively increased the light beam by the absorptive path length of reactant, the light beam is the absorbate fully, can fast, accurately detect out human expired gas's biomarker's content.
Description
Technical field
This utility model belongs to expiration molecular diagnosis field, and in particular to the expiration of the biomarker of detection characteristics of contaminated respiratory droplets
The detection means of molecular diagnostic system.
Background technology
In the gas of normal human's exhalation, in addition to nitrogen, oxygen and carbon dioxide, other many kinds of chemical combination are also included
Thing, by detecting the content of these compounds by diagnose the illness.
The achievement in research that Swiss Zurich Federal Institute of Technology announces recently shows, what everyone was breathed out in breathing
Compound is unique as the fingerprint of the mankind, and doctor even can diagnose the illness according to these compounds.Simultaneously as
Breath tester method can also obtain the result close with uroscopy and blood test, therefore future not only can be used to diagnose disease
Disease, it is also possible to be used in athlete's drug-testing.It is miscellaneous that the research achievement has been published in internal authority biology kind science
Will《PLoS ONE》On.
In American-European countries, expiration molecular diagnosis have become the golden mark technology of respiratory system and digestive system, and for cardiopulmonary,
The medical research and Clinical Laboratory of the diseases such as the intestines and stomach, kidney liver, diabetes and cancer, is desired to commonly encountered diseases, frequently-occurring disease, chronic
Basic unit's examination and family's self-inspection of disease, epidemic diseases and occupation disease.
Due to the biomarker concentration in characteristics of contaminated respiratory droplets gas it is extremely low, with ppb i.e. 1,000,000,000/several as unit meter, accurately
Determine its concentration extremely difficult.
Current breast rail instrument still depends on big and expensive instrument, such as gas chromatograph (GC) and mass spectrograph
(MS) so that these instruments widely can not be applied.
Breast rail instrument also is with detection means made by Electrochemical Detection principle, although small volume but cannot
Realize the limitation such as continuous dynamic monitoring biomarker, testing cost height, poor specificity, it is impossible to fully meet clinical demand.
Utility model content
The technical problems to be solved in the utility model is to provide and a kind of can quick and precisely detect biomarker content
The reflective optical detection device of intelligent expiration molecular diagnostic system.
In order to solve above-mentioned technical problem, a kind of technical scheme that this utility model is adopted is：Intelligent expiration molecular diagnosis
The reflective optical detection device of system, for detecting the content of the biomarker in characteristics of contaminated respiratory droplets gas.The intelligence is exhaled
The reflective optical detection device of gas molecular diagnostic system includes that reaction tank, biosensor and the light path for spectrum analyses are tied
Structure, the biosensor include the reactant that can be reacted with biomarker and absorb biomarker, the reaction
Reaction chamber is provided with pond, the reaction chamber can connect the characteristics of contaminated respiratory droplets gas source of outside, the biosensor installing
At reaction chamber and reactant is encapsulated in reaction intracavity, the reaction tank and biosensor are located at the light of the light channel structure
In beam propagation path, the light channel structure can produce light beam and inject reaction intracavity and through biosensor, the biological biography
Light beam multiple reflections can be made light beam that reaction tank is projected after repeatedly passing through reactant by sensor, and the light channel structure can be by penetrating
The light beam for going out reaction tank obtains the absorption spectrum of reactant.
Specifically, the biosensor also includes matrix, and the reactant is fixedly mounted on matrix, the reaction chamber
The entrance with ft connection is formed with reaction tank, the reactant stretches into reaction intracavity from porch, and described matrix is sealed on
Reactant is enclosed in into reaction intracavity on the entrance of reaction tank.
Specifically, reactant structure in the form of sheets, the reactant have and only a piece of, and the thickness of the reactant is little
In equal to 1mm.
Preferably, described matrix is coated with reflectance coating towards on the end face of the reaction chamber, and the reactant is attached to instead
Penetrate on film, the light channel structure is injected the light beam of reaction chamber and can again pass through reaction through Jing reflectance coatings reflection after reactant
Body.
Further, the reaction tank is the transparent body that can be passed through for light beam, and the inwall of the reaction chamber is cambered surface, institute
Reaction tank is stated also with the outer face matched with reaction cavity wall, cambered surface of the outer face in evagination, the light channel structure are penetrated
The light beam for going out the outer face of reaction tank described in Jing and the inwall of reaction chamber can be incident on the reactant of reaction intracavity successively, institute
State reaction tank and the light beam that reaction chamber is projected after reactant can be led into light channel structure relaying and resume and broadcast.
Further, the reaction tank is concentric ball cover, and the inwall of the reaction chamber and the outer face are concentric
Sphere, the centre of sphere of the sphere are located on reflectance coating.
Specifically, the light channel structure include producing the light source of light beam, the first collimating lens, the first condenser lenses,
Second collimating lens, reflecting mirror, the second condenser lenses, slit, the 3rd collimating lens, grating, tertiary focusing lens and light electrical resistivity survey
Device is surveyed, inject reaction tank in successively by the first collimating lens of Jing and the first condenser lenses for the light beam that the light source is produced, autoreaction pond
The light beam of outgoing the second collimating lens of Jing, reflecting mirror and the second condenser lenses are focused at slit successively, from slit outgoing
The 3rd collimating lens of light beam Jing are reached at grating, light beam diffraction Jing tertiary focusings lens imaging is in light electrical resistivity survey at the grating
Survey the absorption spectrum that reactant is obtained on device.
Specifically, the optical axis of first collimating lens, the optical axis coincidence of the first condenser lenses and both optical axises pass through
The centre of sphere of the sphere of reaction tank.
Specifically, the centre of sphere of sphere of the optical axis of second collimating lens by reaction tank.
Preferably, the reactant includes carrier and cytochrome C, and the carrier is aerosol, the cytochrome C point
Cloth forms the reactant of nanostructured in aerosol.
Scope of the present utility model, however it is not limited to the technical scheme of the particular combination of above-mentioned technical characteristic, while
Should cover other technical schemes for combination in any being carried out and being formed by above-mentioned technical characteristic or its equivalent feature.Such as features described above
Technical scheme replaced mutually and formed with the technical characteristic with similar functions disclosed herein etc..
As above-mentioned technical proposal is used, this utility model has following advantages compared with prior art：Intelligence is exhaled and is divided
The reflective optical detection device of sub- diagnostic system is using the biology in the gas of the reactant and characteristics of contaminated respiratory droplets of biosensor
Mark reacts and the reaction after it can carry out the light channel structure of spectrum analyses to detect organism-absorbing mark to reactant
Body, biosensor reflection make light beam multipass reactant, effectively increase the path that light beam is absorbed by reactant, light
Beam fully can be absorbed.This utility model can quickly and accurately detect containing for the biomarker of characteristics of contaminated respiratory droplets gas
Amount, high precision, sensitivity are high, good reliability.
Description of the drawings
Fig. 1 is that the light channel structure of the reflective optical detection device of this utility model intelligence expiration molecular diagnostic system is illustrated
Figure；
Structural representations of the Fig. 2 for biosensor；
Structural representations of the Fig. 3 for reaction tank；
Wherein：1st, light source；2nd, the first collimating lens；3rd, the first condenser lenses；4th, the second collimating lens；5th, reflecting mirror；6、
Second condenser lenses；7th, slit；8th, the 3rd collimating lens；9th, grating；10th, tertiary focusing lens；11st, photodetector；100、
Reaction tank；101st, reaction chamber；102nd, inwall；103rd, outer face；200th, biosensor；201st, reactant；202nd, matrix；203、
Reflectance coating.
Specific embodiment
As shown in Figure 1 to Figure 3, the reflective optic detection dress of intelligent expiration molecular diagnostic system described in the utility model
Put, for detecting the content of the biomarker in characteristics of contaminated respiratory droplets gas.The reflective optic of intelligent expiration molecular diagnostic system
Detection means includes reaction tank 100, biosensor 200 and the light channel structure for spectrum analyses.The reaction tank 100 and life
Thing sensor 200 is located in the propagation path of the light channel structure.
Biomarker specifically described herein refers to the compound in characteristics of contaminated respiratory droplets gas, clinically it is widely recognized that and being used for
The mark for diagnosing the illness.The such as characteristics of contaminated respiratory droplets such as nitric oxide, carbon monoxide, hydrogen, methane, ammonia, aldehyde and sulfide
Compound or expiration molecule.
The biosensor 200 includes the reactant that can be reacted with biomarker and absorb biomarker
201 and matrix 202.The reflectance coating 203 that can be totally reflected is coated with described matrix 202.The structure in the form of sheets of the reactant 201,
The reactant 201 has and only a piece of, and the thickness of the reactant 201 is less than or equal to 1mm.The reactant 201 is attached to
On reflectance coating 203.The reactant 201 of the present embodiment includes carrier and cytochrome C.The carrier be aerosol, aerosol lead to
Cross sol-gal process to make, the cytochrome C is distributed in aerosol the reactant 201 for forming nanostructured.In order to control
The thickness of reactant 201, forms the cavity volume mould for accommodating reactant 201 by molding.Allow aerosol and cell color in cavity volume
Plain C sizings, so, the THICKNESS CONTROL of reactant 201 is in below 1mm.
The reaction tank 100 is the transparent body that can be passed through for light beam, is provided with reaction chamber 101 in the reaction tank 100,
The reaction chamber 101 can connect the characteristics of contaminated respiratory droplets gas source of outside, i.e. reaction tank 100 and can exhale the human body of outside collection
The sample for going out gas is imported in reaction chamber 101.The reaction chamber 101 is formed with the entrance with ft connection in reaction tank 100.Institute
The inwall 102 for stating reaction chamber 101 is cambered surface, and the reaction tank 100 is also with the outer face matched with 101 inwall 102 of reaction chamber
103, cambered surface of the outer face 103 in evagination.In the present embodiment, the reaction tank 100 be concentric ball cover, the reaction chamber
101 inwall 102 and the outer face 103 are concentric sphere, and the centre of sphere of the sphere is on reflectance coating 203.
The biosensor 200 is installed at reaction chamber 101 and reactant 201 is encapsulated in reaction chamber 101.Specifically
Say that the reactant 201 is stretched in reaction chamber 101 from porch, and described matrix 202 is sealed on the entrance of reaction tank 100 in ground
Reactant 201 is enclosed in reaction chamber 101.So, the end face orientating reaction chamber of reflectance coating 203 is coated with matrix 202
101.So, the biomarker in characteristics of contaminated respiratory droplets gas fully can be marked biological with the cytochrome C reaction in reactant
Will thing absorbs internal in reaction.
The light channel structure includes producing the light source 1 of light beam, the first collimating lens 2, the first condenser lenses 3, second
Collimating lens 4, reflecting mirror 5, the second condenser lenses 6, slit 7, the 3rd collimating lens 8, grating 9, tertiary focusing lens 10 and light
Electric explorer 11.The optical axis of first collimating lens 2, the optical axis coincidence of the first condenser lenses 3 and both optical axises are by anti-
Answer the centre of sphere of the sphere in pond 100.The centre of sphere of sphere of the optical axis of second collimating lens 4 by reaction tank 100.
The first collimating lens of Jing 2 are divided into multi beam directional light, then Jing first to the light beam of the polychromatic light that the light source 1 is produced successively
After condenser lenses 3 are focused on, the outer face 103 in autoreaction pond 100 is injected in reaction tank 100, the inwall 102 in the reacted pond of light beam 100
Penetrate in reaction chamber 101.Light beam in reaction chamber 101 penetrates reactant 201 and focuses on reflectance coating 203, and reflected film
203 reflections again pass through reactant 201 and penetrate on the inwall 102 of reaction chamber 101.Light beam is then along inwall 102 and outer face 103
Project reaction tank 100.The second collimating lens of Jing 4 are divided into directional light to the light beam of 100 outgoing of autoreaction pond successively, and reflected mirror 5 is anti-
Penetrate, the second condenser lenses of Jing 6 are focused at slit 7.Reach at grating 9 from the 3rd collimating lens of light beam Jing 8 of 7 outgoing of slit.
Light beam diffraction Jing tertiary focusings lens 10 are imaged on acquisition biosensor 200 on photodetector 11 at the grating 9
Reactant 201 absorption spectrum.
Reaction after the spectrum and organism-absorbing mark for comparing the reactant 201 before non-organism-absorbing mark
The absorption spectrum of body 201, the content of the biomarker being obtained in that in characteristics of contaminated respiratory droplets gas.Accuracy, precision are very high,
Sensitivity and reliability are also very good.
In biosensor, the laminated structure of reactant 201 makes the thickness of thin of reactant 201, can quickly and biological marker
Thing reacts, and improves the speed of detection.Absorptance with the time is related to the thickness of the reactant that light is passed through, the thickness that light is passed through
It is more thick more can guarantee that the absorbed adequacy of light, so setting reflectance coating 203 by the plating on matrix 202, effectively light beam is guided
Reactant 201 is repeatedly passed through, the path that reactant 201 absorbs light beam is increased, 201 THICKNESS CONTROL of reactant must compared
The path of the absorption light beam of the reactant 201 that light is passed through is can also ensure that on the basis of relatively thin, detection is substantially increased
Sensitivity and reliability.In fact, biosensor 200 and reaction tank 100 can also be arranged as required to it is multiple with reflection
The end face of film 203, increases light beam through the number of times of reactant 201, and then on the basis of the thickness of control reactant 201, root
According to the path for needing increase reactant 201 to absorb light beam, it is ensured that the sensitivity of detection and reliability.
As described above, we are illustrated fully according to objective of the present utility model, but this utility model not limits to
In above-described embodiment and implementation.The practitioner of correlative technology field can be in the scope of technological thought of the present utility model license
Different changes and enforcement are carried out inside.
Claims (10)
1. the reflective optical detection device of intelligent expiration molecular diagnostic system, for detecting the biological mark in characteristics of contaminated respiratory droplets gas
The content of will thing, it is characterised in that：Including reaction tank（100）, biosensor（200）Tie with the light path for spectrum analyses
Structure, the biosensor（200）Including the reactant that can be absorbed with biomarker reaction and by biomarker
（201）, the reaction tank（100）Reaction chamber is provided with inside（101）, the reaction chamber（101）The human body that outside can be connected is exhaled
Go out gas source, the biosensor（200）It is installed in reaction chamber（101）Place and reactant（201）It is encapsulated in reaction chamber
（101）It is interior, the reaction tank（100）And biosensor（200）It is in the propagation path of the light channel structure, described
Light channel structure can produce light beam and inject reaction chamber（101）It is interior and through biosensor（200）, the biosensor
（200）Light beam can be made repeatedly to pass through reactant light beam reflection（201）After project reaction tank（100）, the light channel structure energy
It is enough to pass through to project reaction tank（100）Light beam obtain reactant（201）Absorption spectrum.
2. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 1, it is characterised in that：
The biosensor（200）Also include matrix（202）, the reactant（201）It is fixedly mounted on matrix（202）On, it is described
Reaction chamber（101）In reaction tank（100）It is formed with the entrance with ft connection, the reactant（201）Stretch into instead from porch
Answer chamber（101）It is interior, described matrix（202）It is sealed on reaction tank（100）Entrance on by reactant（201）It is enclosed in reaction chamber
（101）It is interior.
3. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 2, it is characterised in that：
The reactant（201）Structure in the form of sheets, the reactant（201）Have and only a piece of, the reactant（201）Thickness it is little
In equal to 1mm.
4. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 3, it is characterised in that：
Described matrix（202）Towards the reaction chamber（101）End face on be coated with reflectance coating（203）, the reactant（201）Attach
In reflectance coating（203）On, the light channel structure injects reaction chamber（101）Light beam can pass through reactant（201）By reflection
Film（203）Reflection again passes through reactant（201）.
5. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 4, it is characterised in that：
The reaction tank（100）For the transparent body that can be passed through for light beam, the reaction chamber（101）Inwall（102）It is for cambered surface, described
Reaction tank（100）Also have and reaction chamber（101）Inwall（102）The outer face of matching（103）, the outer face（103）In outer
Convex cambered surface, the light beam that the light channel structure is projected being capable of reaction tanks described in Jing successively（100）Outer face（103）And reaction chamber
（101）Inwall（102）It is incident on reaction chamber（101）Interior reactant（201）On, the reaction tank（100）To can pass through
Reactant（201）After project reaction chamber（101）Light beam lead into light channel structure relaying and resume and broadcast.
6. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 5, it is characterised in that：
The reaction tank（100）For concentric ball cover, the reaction chamber（101）Inwall（102）With the outer face（103）For concentric
Sphere, the centre of sphere of the sphere are located at reflectance coating（203）On.
7. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 6, it is characterised in that：
The light channel structure includes the light source that can produce light beam（1）, the first collimating lens（2）, the first condenser lenses（3）, it is second accurate
Straight lens（4）, reflecting mirror（5）, the second condenser lenses（6）, slit（7）, the 3rd collimating lens（8）, grating（9）, tertiary focusing
Lens（10）And photodetector（11）, the light source（1）The light beam of generation the first collimating lens of Jing successively（2）Focus on first
Lens（3）Inject reaction tank（100）It is interior, autoreaction pond（100）Outgoing light beam the second collimating lens of Jing successively（4）, reflection
Mirror（5）With the second condenser lenses（6）Focus on slit（7）Place, from slit（7）The 3rd collimating lens of light beam Jing of outgoing（8）Arrive
Up to grating（9）Place, the light beam is in grating（9）Place's diffraction Jing tertiary focusing lens（10）It is imaged on photodetector（11）On
Obtain reactant（201）Absorption spectrum.
8. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 7, it is characterised in that：
First collimating lens（2）Optical axis, the first condenser lenses（3）Optical axis coincidence and both optical axis pass through reaction tank
（100）Sphere the centre of sphere.
9. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 7, it is characterised in that：
Second collimating lens（4）Optical axis pass through reaction tank（100）Sphere the centre of sphere.
10. the reflective optical detection device of intelligent expiration molecular diagnostic system according to claim 1, its feature exist
In：The reactant（201）Including carrier and cytochrome C, the carrier is aerosol, and the cytochrome C is distributed in gas
The reactant of nanostructured is formed in colloidal sol（201）.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621206304.4U CN206132772U (en) | 2016-11-09 | 2016-11-09 | Reflective optical detection device of intelligence expiration molecular diagnosis system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621206304.4U CN206132772U (en) | 2016-11-09 | 2016-11-09 | Reflective optical detection device of intelligence expiration molecular diagnosis system |
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| CN206132772U true CN206132772U (en) | 2017-04-26 |
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| CN201621206304.4U Active CN206132772U (en) | 2016-11-09 | 2016-11-09 | Reflective optical detection device of intelligence expiration molecular diagnosis system |
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