CN206074509U - A kind of pulsed field gel electrophoresis supply unit - Google Patents

A kind of pulsed field gel electrophoresis supply unit Download PDF

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Publication number
CN206074509U
CN206074509U CN201620757496.1U CN201620757496U CN206074509U CN 206074509 U CN206074509 U CN 206074509U CN 201620757496 U CN201620757496 U CN 201620757496U CN 206074509 U CN206074509 U CN 206074509U
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unit
gel electrophoresis
pulsed field
field gel
cabinet
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王晓平
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Beijing Liuyi Biotechnology Co Ltd
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Beijing Liuyi Biotechnology Co Ltd
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Abstract

This utility model applies to separate the pulsed field gel electrophoresis supply unit in the pulsed field gel electrophoresis system of DNA macromolecular glucos.The pulsed field gel electrophoresis supply unit includes:Main control unit, touch screen man-machine interaction unit, internal communication unit, external communication unit, data record access unit, main power source unit, electrode supply unit, temperature in chassis Monitoring and Controlling unit, and cabinet and its interface.This utility model breaches traditional conventional configuration, and upgrading optimizes man-machine interaction unit, electrode supply unit, and temperature in chassis is monitored and controlled unit, and cabinet;External communication unit is increased, it is convenient to use and for the analysis of experimental result in operator.

Description

A kind of pulsed field gel electrophoresis supply unit
Technical field
The utility model relates to the equipment technical field of separation detection DNA, especially separation detection DNA macromole piece Pulsed field gel electrophoresis supply unit in the pulsed field gel electrophoresis device of section.
Background technology
Common agarose gel electrophoresis technology can separate the nucleic acid of small molecule segment, but for length is more than 40kb DNA macromolecular glucos may efficiently separate in the electrophoresis of constant field intensity, pulsed field gel electrophoresiss solve this A difficult problem, by the change of direction of an electric field, and can make the adjustment of the pulse period of the change of direction of an electric field, separate macromolecular complex Matter.In recent years, with the development of genomics, it is desirable to unrestricted choice between 0-360 ° of the vector angle of electric field, jaw type equilibrium electric field (CHEF) electrophoresis, electric field are inverted the way of outputs such as (FIGE) electrophoresis, and the application of these technologies has greatly promoted pulsed field gel electrophoresis The application of technology, but while requirement more and more higher to the power supply in pulsed field gel electrophoresis equipment especially pulsed field gel electrophoresis device. At present, pulsed field gel electrophoresis device majority is made up of pulsed field gel electrophoresis groove, pulse field ionization source, buffer cooling and circulating device. Adopt the electrode of hexagon more pulsed field gel electrophoresis groove, electrophoretic buffer, external cooling dress and circulating device are held in groove, to pulse Buffer in the electrophoresis tank of field carries out cooling circulation, and in maintenance electrophoresis process, temperature is constant.Pulse field ionization source has display and controls Molding block, is capable of achieving to the setting of electrophoretic procedures, the function such as stores, calls, controlling.
The defect of prior art is:On market, existing pulsed field gel electrophoresis supply unit can not realize telecommunication and prison Survey and control function;And there is no touch-screen input and the human-computer interaction interface function of showing.
Utility model content
This utility model increased the remotely control of pulsed field gel electrophoresis supply unit and log function, can pass through people The setting of work smart machine control program, records the various data of electrophoresis process, analysis of the convenient use person to data result;And And man machine interface is upgraded to touch-screen input and display module;And allow electrophoresis tank to be placed on the pulsed field electricity of energy waterproof sprinkle Above source, it is also possible to pulse field ionization source edge-on placement, so as to reduce the occupancy of desktop area.
Pulsed field gel electrophoresis supply unit is made up of multiple functional units, and is uniformly controlled by main control unit, by respective phase Interface is answered to connect, including:Main control unit, touch screen man-machine interaction unit, internal communication unit, external communication unit, data note Record access unit, main power source unit, electrode supply unit, and cabinet and its interface;Wherein:
Main control unit, including microprocessor and its peripheral cell, realize storage, the operation of primary control program, are stored in and read User's setup parameter, process operation data, and be that internal communication module and external communication module provide data access, and for outer Enclose detection, communication and the control of unit;
External communication unit, by communication and power supply line interface and main control unit and the people outside pulsed field gel electrophoresis system Work smart machine is connected, and realizes the data interaction between pulsed field gel electrophoresis supply unit and artificial intelligence's equipment;
Main power source unit, is that electrode supply unit and the unit in addition to cabinet are powered;
Electrode control unit, carries out respective independent control and switching to the voltage of each iontophoretic electrode;
Touch screen man-machine interaction unit, is the display screen for possessing touch-input function, by communication and power supply line and master Control unit is connected;
Internal communication unit, by communication and power supply line by the electrophoresis tank phase in main control unit and pulsed field gel electrophoresis system Even, pulsed field gel electrophoresis supply unit and the electrophoresis tank in pulsed field gel electrophoresis system, and the pulsed field being connected with electrophoresis tank are realized The data interaction of other modules in electrophoresis system;
Above each unit is each independent to be connected with each other form, or combination at least two circuit boards or hardware mould On block;
The cabinet and its interface of pulsed field gel electrophoresis power supply, provides protection installing space with radiating for unit in cabinet Function.
Electrode control unit, realizes the independent control to every electrode in pulsed field gel electrophoresis groove, according to each electrode in arteries and veins Rush relative position residing in uniform electric field formed in the electrophoresis tank of field and give corresponding appropriate voltage, each electrode is made in electrophoresis Cooperate in groove, form one uniformly, according to the impulse electric field of the angle and time change of setting.
Touch screen man-machine interaction unit, carries out data and word input by the display screen for possessing touch-input function, and Main control unit is transferred to, and the virtual voltage of main control unit upload can be shown.
External communication unit, by the way of data interaction, by communication and power supply line interface, with main control unit and arteries and veins The artificial intelligence's equipment rushed outside the electrophoresis system of field is connected, and realizes artificial intelligence's equipment to pulse field ionization source and its connected pulse In the electrophoresis power device of field, the data communication and control of temperature control and loop control module and electrophoresis tank, carries out various experiment journeys The parameter setting and data transfer of sequence.
Internal communication unit, by communication and power supply line interface by the electrophoresis in main control unit and pulsed field gel electrophoresis system Groove is connected, and realizes pulsed field gel electrophoresis supply unit and the electrophoresis tank in pulsed field gel electrophoresis system, and the arteries and veins being connected with electrophoresis tank The data interaction of other modules rushed in the electrophoresis system of field, is connected with ancillary equipment, and transmitted in both directions simultaneously preserves data.
Cabinet of the pulsed field gel electrophoresis supply unit also including pulse field ionization source, its bottom and the left and right sides have at least 1 group, often At least 4 footing, horizontal positioned, or the left side or the right are organized for bottom, edge-on placement;Accordingly, touch screen man-machine interaction list Unit, its display content can at least be rotated by 90 °.
The cabinet of pulse field ionization source, in horizontal positioned, each side size of cabinet top surface is more than electrophoresis tank bottom supporting portion The size placed needed for part, can be placed horizontally in supporting electrophoresis tank above the cabinet of pulse field ionization source.
The cabinet of pulse field ionization source, the air inlet of cooling system are arranged on cabinet bottom, the front panel of enclosure top cover plate Rain visor is respectively equipped with above top and enclosure top air exhausting device below;The cabinet tapping of touch screen man-machine interaction unit Also post transparent watertight panel film.
In pulsed field power case, the air inlet of cooling system is provided with hole pattern shape bracket, and its with cabinet air intake perforate Space between region is provided with air-filtering layer part.
Pulse field ionization source, also including temperature in chassis Monitoring and Controlling unit, according to the temperature in chassis for measuring, control radiating The operating of fan.
Description of the drawings
Fig. 1 is the one preferential embodiment of pulsed field gel electrophoresis supply unit described in the utility model:
Including:1 main control unit, 2 external communication units, 3 touch screen man-machine interaction units, 4 main power source units, 5 electrode controls Unit processed, communication unit in 6 pairs, 7 cabinets.
Fig. 2 is the cabinet front perspective view of one preferred version of pulse field ionization source described in the utility model.
Wherein:3 touch screen man-machine interaction units, 8 front panels, 9 chassis cover side cover plates, the cabinet of 10 external communication units Opening, 11 cabinet footing, rain visor before 12, rain visor after 13.
Fig. 3 is the cabinet rear perspective view of one preferred version of pulse field ionization source described in the utility model.
11 cabinet footing, 13 are opened with the rear rain visor of chassis cover one, 14 radiator fan perforates and radome, 15 power supplys Close, 16 AC power interfaces, 17 electrophoresis tank DC sources are exported and communication interface, 19 cabinet rear boards.
Specific embodiment
In order to improve the service efficiency of pulsed field gel electrophoresis supply unit, optimization experiment result increases in this utility model The remotely control and log function of power supply, can pass through artificial intelligence's equipment and logical with electrophoresis field ionization source communication module News, remote setting source program operational factor;It is thereby achieved that the overall process of electrophoresis experiment is monitored and controlled, such that it is able to Intelligentized control method and digitized experimentation are realized, is played in interpretation, especially collimation interpretation Important effect;And, improve cabinet and filter ventilation and heat structure, and cabinet waterproof construction, be easy to arteries and veins is carried on cabinet Rush field electrophoresis tank;Cabinet side increased anti-skidding feet and cabinet inside has made corresponding adjustment, and due to defeated with touching Enter the display screen of function and be rotated by 90 ° or 270 degree of function while also having, allow cabinet not carrying pulsed field gel electrophoresis groove When, edge-on placement reduces desktop area occupied.
To make the purpose of this utility model, technical scheme and advantage clearer, below with a preferential case study on implementation, With reference to Figure of description, it is described in further details.
Pulsed field gel electrophoresis supply unit as shown in Figure 1 to Figure 3, the pulsed field gel electrophoresis supply unit subdivision control, Connected by respective the corresponding interface, including:
Main control unit 1, touch screen man-machine interaction unit 3, internal communication unit 6, external communication unit 2, main power source unit 4, electrode control unit 5, and cabinet 7 and its interface;Wherein:
Main control unit 1, including microprocessor and its peripheral cell, realizes storage, the operation of primary control program, is stored in and reads User's setup parameter, process operation data, and be that internal communication unit 6 and external communication unit 2 provide data access, and for Detection, communication and the control of peripheral unit;
External communication unit 2, outside communication and power supply line interface and main control unit 1 and pulsed field gel electrophoresis system Artificial intelligence's equipment is connected, and realizes the data interaction between pulsed field gel electrophoresis supply unit and artificial intelligence's equipment;
Main power source unit 4, is that electrode supply unit and the unit in addition to cabinet are powered;
Electrode control unit 5, carries out respective independent control and switching to the voltage of each iontophoretic electrode;
Touch screen man-machine interaction unit 3, is the display screen for possessing touch-input function, by communication and power supply line and master Control unit is connected;
Internal communication unit, by communication and power supply line by the electrophoresis tank phase in main control unit and pulsed field gel electrophoresis system Even, pulsed field gel electrophoresis supply unit and the electrophoresis tank in pulsed field gel electrophoresis system, and the pulsed field being connected with electrophoresis tank are realized The data interaction of other modules in electrophoresis system;
Above each unit can be each it is independent be connected with each other form, or combination at least two circuit boards or In hardware module;
The cabinet 7 of pulsed field gel electrophoresis power supply and its interface, are unit in cabinet, and may be pulsed field gel electrophoresis system Buffer temperature control modules and buffer circulating cooling module in system also merge the cabinet for being installed to pulsed field gel electrophoresis power supply In, there is provided the function of enough protection installing spaces and radiating.
Electrode control unit 5, is capable of achieving the independent control to every electrode in pulsed field gel electrophoresis groove, is existed according to each electrode Formed in pulsed field gel electrophoresis groove, relative position residing in uniform electric field gives corresponding appropriate voltage, makes each electrode in electricity Cooperate in swimming groove, form one uniformly, according to the impulse electric field of the angle and time change of setting.
Touch screen man-machine interaction unit 3, can carry out data by the display screen for possessing touch-input function and word is defeated Enter, and be transferred to main control unit 1, and the parameters such as the virtual voltage of the upload of main control unit 1 can be shown.
The external communication unit 2 of pulsed field gel electrophoresis power supply, by the way of data interaction, is connect by communication and power supply line Mouthful, it is connected with the artificial intelligence's equipment outside main control unit 1 and pulsed field gel electrophoresis system, realizes artificial intelligence's equipment to pulsed field In power supply and its connected pulsed field gel electrophoresis supply unit the data communication of temperature control and loop control module and electrophoresis tank and Control, carries out the parameter setting and data transfer of various experimental arrangements.
The internal communication unit 6 of pulse field ionization source, it is main control unit 1 is electric with pulsed field by communication and power supply line interface Electrophoresis tank in swimming system is connected, and realizes pulsed field gel electrophoresis supply unit and the electrophoresis tank in pulsed field gel electrophoresis system, Yi Jiyu The data interaction of other modules in the pulsed field gel electrophoresis system that electrophoresis tank is connected, is connected with ancillary equipment, and transmitted in both directions is simultaneously protected Deposit data.
The cabinet 7 of pulse field ionization source, its bottom and the left and right sides have at least 1 group, per group of at least 4 cabinet footing 11, can With horizontal positioned, it is also possible to which the left side or the right are bottom, edge-on placement;Accordingly, the touch screen people with touch-input function Machine interactive unit 3, its display content can at least be rotated by 90 °.
The cabinet 7 of pulse field ionization source, in horizontal positioned, each side size of cabinet top surface is more than electrophoresis tank bottom supporting portion The size placed needed for part, can be placed horizontally in supporting electrophoresis tank above the cabinet 7 of pulse field ionization source.
The cabinet 7 of pulse field ionization source, the air inlet of cooling system are arranged on cabinet bottom, before chassis cover side cover plate 9 Front rain visor 12 and rear rain visor 13 are respectively equipped with the top of cabinet rear board 19 above panel 8;Touch screen man-machine interaction list The cabinet tapping of unit 3 also posts transparent watertight panel film.
In pulsed field power case 7, the air inlet of cooling system is provided with hole pattern shape bracket, and its with cabinet air intake perforate Space between region can be provided with air-filtering layer part.
Pulse field ionization source, can increase temperature in chassis Monitoring and Controlling unit, according to the temperature in chassis for measuring, control radiating The operating of fan.
This utility model is not limited to above-mentioned preferred implementation, and anyone can be drawn under enlightenment of the present utility model Other various forms of products, however, make any change in its shape or structure, it is every with same as the present application or phase Approximate technical scheme, all falls within protection domain of the present utility model.

Claims (11)

1. a kind of pulsed field gel electrophoresis supply unit, the pulsed field gel electrophoresis supply unit include:Main control unit, the man-machine friendship of touch screen Mutual unit, internal communication unit, external communication unit, data record access unit, main power source unit, electrode supply unit, and Cabinet and its interface;Wherein:
Main control unit, including microprocessor and its peripheral cell, realize storage, the operation of primary control program, are stored in and read user Setup parameter, process operation data, and be that internal communication module and external communication module provide data access, and it is each for periphery Detection, communication and the control of individual unit;
External communication unit, by communication and power supply line interface and main control unit and the artificial intelligence outside pulsed field gel electrophoresis system Energy equipment is connected, and realizes the data interaction between pulsed field gel electrophoresis supply unit and artificial intelligence's equipment;
Main power source unit, is that electrode supply unit and the unit in addition to cabinet are powered;
Electrode control unit, carries out respective independent control and switching to the voltage of each iontophoretic electrode;
Touch screen man-machine interaction unit, is the display screen for possessing touch-input function, by communication and power supply line and master control list Unit is connected;
Internally main control unit is connected with the electrophoresis tank in pulsed field gel electrophoresis system by communication unit by communication and power supply line, Realize pulsed field gel electrophoresis supply unit and the electrophoresis tank in pulsed field gel electrophoresis system, and the pulsed field gel electrophoresis being connected with electrophoresis tank The data interaction of other modules in system;
Above each unit is each independent to be connected with each other form, or combination at least two circuit boards or hardware module On;
The cabinet and its interface of pulsed field gel electrophoresis power supply, provides the work(of protection installing space and radiating for unit in cabinet Energy.
2. pulsed field gel electrophoresis supply unit according to claim 1, it is characterised in that:Electrode control unit is realized to pulse The independent control of every electrode in electrophoresis tank, according to each electrode formed in pulsed field gel electrophoresis groove in uniform electric field residing for Relative position give corresponding appropriate voltage, make each electrode cooperate in electrophoresis tank, formed one it is uniform, according to The impulse electric field of the angle and time change of setting.
3. pulsed field gel electrophoresis supply unit according to claim 1, it is characterised in that:The touch screen human-computer exchange unit Data and word input are carried out by the display screen for possessing touch-input function, and is transferred to main control unit, and master can be shown The virtual voltage that control unit is uploaded.
4. pulsed field gel electrophoresis supply unit according to claim 1, it is characterised in that:The external communication unit adopts number According to interactive mode, by communication and the artificial intelligence outside power supply line interface, with main control unit and pulsed field gel electrophoresis system Equipment is connected, realize artificial intelligence's equipment to temperature control in pulse field ionization source and its connected pulsed field gel electrophoresis supply unit and The data communication and control of loop control module and electrophoresis tank, carries out the parameter setting and data transfer of various experimental arrangements.
5. pulsed field gel electrophoresis supply unit according to claim 1, it is characterised in that:The internal communication unit is by logical Main control unit is connected by news and power supply line interface with the electrophoresis tank in pulsed field gel electrophoresis system, realizes that pulsed field gel electrophoresis power supply is filled Put and the electrophoresis tank in pulsed field gel electrophoresis system, and the number of other modules in the pulsed field gel electrophoresis system being connected with electrophoresis tank According to interaction, it is connected with ancillary equipment, transmitted in both directions simultaneously preserves data.
6. pulsed field gel electrophoresis supply unit according to claim 1, it is characterised in that:Also including the machine of pulse field ionization source Case, its bottom and the left and right sides have at least 1 group, per group of at least 4 footing, horizontal positioned, or the left side or the right are bottom, side Stand up and put.
7. the pulsed field gel electrophoresis supply unit according to claim 1 or claim 4, it is characterised in that the touch Screen man-machine interaction unit, its display content can at least be rotated by 90 °.
8. pulsed field gel electrophoresis supply unit according to claim 6, it is characterised in that:The cabinet of pulse field ionization source, in water During placing flat, each side size of cabinet top surface, can be supporting more than the size placed needed for electrophoresis tank bottom-support part Electrophoresis tank is placed horizontally in above the cabinet of pulse field ionization source.
9. pulsed field gel electrophoresis supply unit according to claim 6, it is characterised in that:The cabinet of pulse field ionization source, radiating The air inlet of system is arranged on cabinet bottom, above the front panel of enclosure top cover plate and on enclosure top air exhausting device below Side is respectively equipped with rain visor;The cabinet tapping of touch screen man-machine interaction unit also posts transparent watertight panel film.
10. pulsed field gel electrophoresis supply unit according to claim 9, it is characterised in that:The air inlet of cooling system sets There is hole pattern shape bracket, and its air-filtering layer part is provided with the space between cabinet air intake opening area.
11. pulsed field gel electrophoresis supply units according to claim 1, it is characterised in that:Also monitor including temperature in chassis Control unit, according to the temperature in chassis for measuring, controls the operating of radiator fan.
CN201620757496.1U 2016-07-19 2016-07-19 A kind of pulsed field gel electrophoresis supply unit Active CN206074509U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620757496.1U CN206074509U (en) 2016-07-19 2016-07-19 A kind of pulsed field gel electrophoresis supply unit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620757496.1U CN206074509U (en) 2016-07-19 2016-07-19 A kind of pulsed field gel electrophoresis supply unit

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111074318A (en) * 2019-11-13 2020-04-28 南京工业大学 Electrophoresis-micro arc oxidation synchronous processing power supply based on grouping pulse

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111074318A (en) * 2019-11-13 2020-04-28 南京工业大学 Electrophoresis-micro arc oxidation synchronous processing power supply based on grouping pulse
CN111074318B (en) * 2019-11-13 2020-11-03 南京工业大学 Electrophoresis-micro arc oxidation synchronous processing power supply based on grouping pulse

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