CN206066590U - A kind of card punch for making of micro-cavity embryo culture - Google Patents

A kind of card punch for making of micro-cavity embryo culture Download PDF

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Publication number
CN206066590U
CN206066590U CN201620702727.9U CN201620702727U CN206066590U CN 206066590 U CN206066590 U CN 206066590U CN 201620702727 U CN201620702727 U CN 201620702727U CN 206066590 U CN206066590 U CN 206066590U
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China
Prior art keywords
electric cautery
top board
pipe
micro
connecting portion
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CN201620702727.9U
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Chinese (zh)
Inventor
杨春艳
尚江华
郑海英
覃广胜
黄芬香
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GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INSTITUTE
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GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INSTITUTE
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Abstract

This utility model provides a kind of card punch for making of micro-cavity embryo culture, is related to test tool technical field, including positioning protective sleeve, electric cautery and extension spring;Positioning protective sleeve includes top board and pipe, offers air-vent on the tube wall of pipe;Top board is arranged on one end of pipe, offers through hole, and parallel with the other end of the pipe;Electric cautery passes through the through hole, and limiting section and connecting portion are provided with the wall of side, and head is in dome-type;Top board is arranged between limiting section and connecting portion, and extension spring is arranged between top board and connecting portion and is connected with top board and connecting portion;This utility model is by arranging the positioning protective sleeve coordinated with electric cautery, and setting limiting section is used for the insertion depth for controlling the electric cautery on the wall of the electric cautery side;Also by the extension spring is arranged between electric cautery and positioning protective sleeve, card punch automatic rebounding after work is made, simultaneously because employing the electric cautery for heating that works, work efficiency is increased.

Description

A kind of card punch for making of micro-cavity embryo culture
Technical field
This utility model is related to test tool technical field, and in particular to it is a kind of for embryo culture with beating that micro-cavity makes Hole device.
Background technology
Currently as a kind of conventional system of embryo culture, numerous studies show which can improve Embryo Production for micro-cavity culture Efficiency;For free-hand clone embryos, the system not only can make the mutual aggressiveness of the blastomere of single clone embryos, Blastocyst rate can be improved by interaction between embryo with multiple reconstructed embryo co-cultivation.Micro-cavity system needs to make 150- at culture dish bottom 180 μm or so of recessed nest, covers per 10 use, 40 μ L culture fluid.
Generally micro-cavity is processed by experimenter oneself.During processing, experimenter needs to follow the steps below:
First, self-made punching pin is heated using alcohol burner;
2nd, perforating needle is heated to culture dish is inserted the needle into after uniform temperature, is made for cultivating the micro- of individual cells Cave.
In this process, as the temperature of perforating needle can not be immediately arrived at, experimenter can only authority micro-judgment The temperature of perforating needle, and as the depth of perforating needle insertion accurately can not ensure, cause the pore size beaten on culture dish not One, it is also possible to punch the base plate of culture dish.Simultaneously as being heated using alcohol burner, temperature drops perforating needle after drilling Heated on the low flame for taking alcohol burner to a certain extent again, compared impact efficiency.
Utility model content
In view of the foregoing, it is necessary to provide a kind of card punch for making of micro-cavity embryo culture, it can either protect The temperature of card perforating needle is more constant, is capable of the depth of precise control punching again, it is ensured that culture dish is not punched, can be beating Heated while hole, increased the efficiency of experimenter.
To reach above-mentioned purpose, the technical scheme adopted by this utility model is:It is a kind of for using micro-cavity system embryo culture The card punch of work, including positioning protective sleeve, electric cautery and extension spring;The positioning protective sleeve includes top board and pipe, the pipe Tube wall on offer air-vent;The top board is arranged on one end of the pipe, offers through hole, and with the pipe The other end is parallel;The electric cautery passes through the through hole, and limiting section and connecting portion are provided with the wall of side, and head is in hemisphere Type;Wire enters the electric cautery from the side wall of the electric cautery afterbody, and connects with the coil of the electric cautery;It is described spacing Portion is parallel to each other with the opposite face of the connecting portion, and perpendicular to the axis of the electric cautery;The limiting section can be stuck in On the top board;The top board is arranged between the limiting section and the connecting portion, and the pipe is located at the connecting portion side, The extension spring is arranged between the top board and the connecting portion and is connected with the top board and the connecting portion;The electricity Flatiron upper limit portion needs drilling to the length+culture dish of the thickness+pipe of the distance=top board of head end Depth;The thickness of the natural length of the extension spring+connecting portion+connecting portion is to the electric cautery head end apart from < The length of the pipe.
Further, the top board is annular shape, and the through hole is the top board centre bore.
Further, the radius of the extension spring more than the through hole half.
Further, the limiting section and the connecting portion are in annular shape, perpendicular to the electric cautery axis, and big footpath More than the radius of the through hole.
Further, the air-vent being opened on the tube wall of the pipe symmetrically.
Further, the electric cautery is thermostatic type electric cautery.
Further, the electric cautery is vertically arranged with the top board.
Further, the axis of the pipe is perpendicular to the top board.
Further, the axis coaxle of the electric cautery and the pipe.
Further, the tail end face of the electric cautery is parallel with the top board, the electric cautery and the via clearance Coordinate.
This utility model has the advantages that:
The positioning protective sleeve that this utility model is coordinated with the electric cautery by setting, and on the wall of the electric cautery side The limiting section is set for controlling the insertion depth of the electric cautery, both ensure that punching depth, and in turn ensure that the electricity was ironed Ferrum is difficult to hinder handss;Also by the extension spring is arranged between the electric cautery and the positioning protective sleeve, card punch is made in work Automatic rebounding, saves the operating procedure of experimenter afterwards, simultaneously because employing the electric cautery for heating that works, increases Work efficiency is added.
Description of the drawings
Fig. 1 is a kind of explosive view of the card punch for micro-cavity making embryo culture of this utility model.
Fig. 2 is a kind of sectional view of the card punch for micro-cavity making embryo culture of this utility model.
The drawing reference numeral explanation of specific embodiment:
Positioning protective sleeve 1 Top board 11 Through hole 111
Pipe 12 Air-vent 121 Electric cautery 2
Limiting section 21 Connecting portion 22 Wire 23
Extension spring 3
Specific embodiment
The punching for making of micro-cavity embryo culture a kind of to this utility model with specific embodiment below in conjunction with the accompanying drawings Device is described further.
Fig. 1-2 is referred to, it is in the middle of one preferred embodiment of this utility model, a kind of for making of micro-cavity embryo culture Card punch includes positioning protective sleeve 1, electric cautery 2 and extension spring 3.
The positioning protective sleeve 1 includes top board 11 and pipe 12, and air-vent 121 is offered on the tube wall of the pipe 12; The top board 11 is arranged on one end of the pipe 12, offers through hole 111, and parallel with the other end of the pipe 12. Preferably, the top board 11 is annular shape, and the through hole 111 is 11 centre bore of the top board, and the axis of the pipe 12 is vertical In the top board 11, the axis coaxle of the electric cautery 2 and the pipe 12, being opened in symmetrically of the air-vent 121 On the tube wall of the pipe 12.
The electric cautery 2 passes through the through hole 111, and limiting section 21 and connecting portion 22 are provided with the wall of side, and head is in Dome-type;Wire 23 enters the electric cautery 2 from the side wall of 2 afterbody of the electric cautery, and connects with the coil of the electric cautery 2 It is logical.The limiting section 21 is parallel to each other with the opposite face of the connecting portion 22, and perpendicular to the axis of the electric cautery 2;Institute State limiting section 21 to be stuck on the top board 11.Preferably, the limiting section 21 and the connecting portion 22 are in annular shape, inwall It is connected with the electric cautery 2, perpendicular to 2 axis of the electric cautery, and big footpath is more than the radius of the through hole 111;The electricity Flatiron 2 is thermostatic type electric cautery.
The top board 11 is arranged between the limiting section 21 and the connecting portion 22, and the pipe 12 is located at the connection 22 side of portion, the extension spring 3 be arranged between the top board 11 and the connecting portion 22 and with the top board 11 and the connection Portion 22 connects.Preferably, the electric cautery 2 is vertically arranged with the top board 11, and the radius of the extension spring 3 is more than the through hole 111 radius.In the present embodiment, the tail end face of the electric cautery 2 is parallel with the top board 11, the electric cautery 2 and institute State the cooperation of 111 gap of through hole.
2 upper limit portion 21 of the electric cautery is to the thickness+pipe 12 of the distance=top board 11 of head end Length+culture dish needs the depth of drilling.
The thickness of the natural length of the extension spring 3+connecting portion 22+connecting portion 22 arrives 2 head of the electric cautery The length apart from pipe described in < 12 at end.
During use, by described for being placed on culture dish base plate, the circle with the card punch that micro-cavity makes embryo culture One end that pipe 12 is not closed and culture dish contacts baseplate, being then powered to the electric cautery 2 by the wire 23 carries out adding Heat.The electric cautery 2 presses down on the afterbody of the electric cautery 2 after heating, until the limiting section 21 is connect with the top board 11 Touch, now the head of the electric cautery 2 stretches out the pipe 12 and the hole of appropriate depth is bored on culture dish base plate, it is ensured that culture Ware base plate will not be drilled through.It is drilled after hole looses one's grip, the electric cautery 2 is retracted original position by the extension spring 3, makes the electric cautery 2 not Experimenter is ironed easily.
The positioning protective sleeve 1 that this utility model is coordinated with the electric cautery 2 by setting, and in 2 side of the electric cautery The limiting section 21 is set on wall for controlling the insertion depth of the electric cautery 2, punching depth had both been ensure that, and in turn ensure that institute State electric cautery 2 to be difficult to hinder handss;Also by the extension spring 3 is arranged between the electric cautery 2 and the positioning protective sleeve 1, make to beat Hole device automatic rebounding after work, saves the operating procedure of experimenter, heats simultaneously because employing and working Electric cautery 2, increased work efficiency.
Described above is the detailed description for the preferable possible embodiments of this utility model, but embodiment is not limited to Patent claim of the present utility model, the equal change completed under the technical spirit suggested by all this utility model or modification Change, all should belong to the covered the scope of the claims of this utility model.

Claims (10)

1. a kind of card punch for making of micro-cavity embryo culture, it is characterised in that:The micro-cavity card punch includes that positioning is protected Sheath, electric cautery and extension spring;
The positioning protective sleeve includes top board and pipe, offers air-vent on the tube wall of the pipe;The top board is arranged on One end of the pipe, offers through hole, and parallel with the other end of the pipe;
The electric cautery passes through the through hole, and limiting section and connecting portion are provided with the wall of side, and head is in dome-type;Wire from The side wall of the electric cautery afterbody enters the electric cautery, and connects with the coil of the electric cautery;The limiting section with it is described The opposite face of connecting portion is parallel to each other, and perpendicular to the axis of the electric cautery;The limiting section can be stuck in the top board On;
The top board is arranged between the limiting section and the connecting portion, and the pipe is located at the connecting portion side, the drawing Spring is arranged between the top board and the connecting portion and is connected with the top board and the connecting portion;
Length+culture dish of the electric cautery upper limit portion to the thickness+pipe of the distance=top board of head end Need the depth of drilling;
The thickness of the natural length of the extension spring+connecting portion+connecting portion is to the electric cautery head end apart from < The length of the pipe.
2. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The top board is circle Ring-type, the through hole are the top board centre bore.
3. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The half of the extension spring Radius of the footpath more than the through hole.
4. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The limiting section and , in annular shape, perpendicular to the electric cautery axis, and big footpath is more than the radius of the through hole for the connecting portion.
5. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The air-vent is equal Even symmetrical is opened on the tube wall of the pipe.
6. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The electric cautery is Thermostatic type electric cautery.
7. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The electric cautery with The top board is vertically arranged.
8. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The axle of the pipe Line is perpendicular to the top board.
9. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The electric cautery with The axis coaxle of the pipe.
10. the card punch for making of micro-cavity embryo culture is used for as claimed in claim 1, it is characterised in that:The electric cautery Tail end face it is parallel with the top board, the electric cautery is coordinated with the via clearance.
CN201620702727.9U 2016-07-01 2016-07-01 A kind of card punch for making of micro-cavity embryo culture Active CN206066590U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620702727.9U CN206066590U (en) 2016-07-01 2016-07-01 A kind of card punch for making of micro-cavity embryo culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620702727.9U CN206066590U (en) 2016-07-01 2016-07-01 A kind of card punch for making of micro-cavity embryo culture

Publications (1)

Publication Number Publication Date
CN206066590U true CN206066590U (en) 2017-04-05

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107592830A (en) * 2016-05-10 2018-01-16 法国圣戈班玻璃厂 Soldering tip for soldering iron

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107592830A (en) * 2016-05-10 2018-01-16 法国圣戈班玻璃厂 Soldering tip for soldering iron
US20190015918A1 (en) * 2016-05-10 2019-01-17 Saint-Gobain Glass France Soldering tip for a soldering iron
US10835979B2 (en) 2016-05-10 2020-11-17 Saint-Gobain Glass France Soldering tip for a soldering iron

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