CN206057337U - A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum - Google Patents
A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum Download PDFInfo
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- CN206057337U CN206057337U CN201621057749.0U CN201621057749U CN206057337U CN 206057337 U CN206057337 U CN 206057337U CN 201621057749 U CN201621057749 U CN 201621057749U CN 206057337 U CN206057337 U CN 206057337U
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Abstract
This utility model is related to tobacco ralstonia solanacearum detection, specifically a kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum, including for detecting the test strips of ralstonia solanacearum, the test strips are made up of base plate and the sample pad being sequentially arranged on base plate, gold conjugation pad, the NC films for being provided with nature controlling line and detection line and absorbent paper, it is characterised in that：The detection means for the spherical container of pre-treatment is carried out to soil, is loaded with tissue preserration liquid, a soil body fluid connecting pipeline is provided between spherical container and the sample pad of test strips also including one in spherical container, the connecting pipeline is provided with valve and filter cotton.This utility model advantage compared to existing technology is that solution mixing and filtration treatment are carried out to tobacco-growing soil, and then next step detection is carried out to body fluid, and very easy to use quick, sensitivity is high, and testing cost is low.It is particularly suitable for the quick detection of tobacco ralstonia solanacearum in soil.
Description
Technical field
This utility model is related to tobacco ralstonia solanacearum detection, specifically a kind of suitable for tobacco-growing soil tobacco ralstonia solanacearum
Detection means.
Background technology：
Tobacco bacterial wilt is by a kind of microbial important vascular bundle diseases of the withered vacation list bag bar of green grass or young crops, in temperate zone and subtropical zones
Main product cigarette country have generation, endanger very big to tobacco leaf production, mainly based on soil-borne.China is mainly flowed in the Changjiang river
Domain and its on the south cigarette district Guangdong, Fujian, Taiwan, Hunan, Jiangxi, east Guangxi, Southern Anhui Province, Sichuan and Guizhou local cigarette district etc.
Ground occurs., Yi Dan occurring to be renovated after symptom occurs again, difficulty is very big for tobacco bacterial wilt, so premorbid detection is extremely closed
Key, before generation, quickly and accurately detection is hidden the quantity of in tobacco planting field soil ralstonia solanacearum, and then in advance to which
Processed, ralstonia solanacearum is controlled to be particularly important within morbidity thresholding.Current detection method mainly has conventional inspection
Survey method, serological method, molecular biology for detection etc..The advantage of Routine assays is simple and easy to do, and which has the disadvantage inspection
The survey cycle is long, and abecedarian's False Rate is high, it is difficult to reach the requirement of " quick, accurate, economic ", molecular biology for detection is such as
Quantitative PCR detection can accurately obtain the quantity of ralstonia solanacearum in soil, but process is complex, and technical difficulty is high, to behaviour
Basic demand is higher.Immunochromatography technique is a kind of relatively simple effective detection method, and its ultimate principle is by short strip shape
Fiber chromatographic material after measuring samples solution is immersed, makes which on fiber chromatography strip by capillarity as solid phase carrier
Mobile, there is high specific immunoreation in the receptor on the thing to be checked in sample and chromatographic material for specific thing to be checked, in layer
During analysis reaction, immune complex is enriched in the detection zone on chromatographic material, and using by enzyme reaction or directly can
The label (such as gold colloidal, latex particle etc.) of range estimation and occur intuitively observing result.Pedotheque to be measured adds for needs
Enter the pedotheque that liquid is mixed is preserved using soil albumen.
At present for the detection of ralstonia solanacearum in soil is difficult to accomplish that sensitive, efficient, this present situation also causes correlation in the industry
The concern of research worker, and carried out further development test.
The content of the invention
The purpose of this utility model is based on above-mentioned prior art situation and develops a kind of suitable for tobacco-growing soil cigarette
The detection means of careless ralstonia solanacearum, the device have sensitivity high, swift to operate, low cost and other advantages, it is adaptable to respectively plant cigarette
Detection work in soil to ralstonia solanacearum.
The purpose of this utility model is achieved through the following technical solutions：It is a kind of withered suitable for tobacco-growing soil Nicotiana tabacum L. green grass or young crops
The detection means of pathogenic bacteria, including for detecting the test strips of ralstonia solanacearum, the test strips are by base plate and are sequentially arranged on base plate
Sample pad, gold conjugation pad are provided with the NC films and absorbent paper composition of nature controlling line and detection line, wherein：The detection means is also wrapped
One is included for the spherical container of pre-treatment is carried out to soil, tissue preserration liquid in spherical container, is loaded with, in spherical container and examination
A soil body fluid connecting pipeline is provided between the sample pad of paper slip, the connecting pipeline is provided with valve and filter cotton.
In this utility model, the communicating pipe between spherical container and the sample pad of test strips is provided with grafting interface tube.
Baton round of the spherical container for diameter 3cm.
This utility model when in use, takes after soil to be measured is crumbed and loads in the spheroid containing tissue preserration liquid, rock mixed
It is even, the connecting tube being connected with test strips sample pad is accessed, valve is opened, soil body fluid enters test strips after filter pulp filtration
Detected, and then whether contained tobacco ralstonia solanacearum in judging the soil.
This utility model advantage compared to existing technology is can to carry out solution mixing and filtration treatment to tobacco-growing soil,
And then next step detection is carried out to soil body fluid, very easy to use quick, sensitivity is high, and testing cost is low.It is particularly suitable for
The quick detection of tobacco ralstonia solanacearum in soil, the preventing and treating to bacterial wilt have certain prevention and control effect.
Description of the drawings
Fig. 1 is structural representation of the present utility model.
In figure：1st, spherical container, 2, grafting interface tube, 3, connecting pipeline, 4, valve, 5, filter cotton, 6, base plate, 7, sample
Pad, 8, gold conjugation pad, 9, nature controlling line, 10, detection line, 11, absorbent paper.
Specific embodiment
This utility model is described further below in conjunction with accompanying drawing：
As shown in Figure 1：A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum, including for detecting bacterial wilt
The test strips of bacterium, the test strips are provided with nature controlling line 9 by base plate 6 and the sample pad 7 being sequentially arranged on base plate, gold conjugation pad 8
Constitute with the NC films of detection line 10 and absorbent paper 11, wherein：The detection means also includes one for carrying out pre-treatment to soil
Spherical container 1（Baton round）, tissue preserration liquid is loaded with spherical container 1, spherical container 1 and test strips sample pad 7 it
Between be provided with a soil body fluid connecting pipeline 3, the connecting pipeline 3 is provided with valve 4 and filter cotton 5.
Connecting pipeline between the sample pad 7 of spherical container 1 and test strips is provided with grafting interface tube 2.
Claims (3)
1. a kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum, including for detecting the test strips of ralstonia solanacearum,
The test strips are provided with the NC films of nature controlling line and detection line by base plate and the sample pad being sequentially arranged on base plate, gold conjugation pad
And absorbent paper is constituted, it is characterised in that：The detection means also includes one for the spherical container of pre-treatment is carried out to soil, spherical
Tissue preserration liquid is loaded with container, a soil body fluid connecting pipeline is provided between spherical container and the sample pad of test strips,
The connecting pipeline is provided with valve and filter cotton.
2. the detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum according to claim 1, it is characterised in that：It is spherical
Connecting pipeline between container and the sample pad of test strips is provided with grafting interface tube.
3. the detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum according to claim 1, it is characterised in that：It is described
Baton round of the spherical container for diameter 3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621057749.0U CN206057337U (en) | 2016-09-18 | 2016-09-18 | A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621057749.0U CN206057337U (en) | 2016-09-18 | 2016-09-18 | A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum |
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| CN206057337U true CN206057337U (en) | 2017-03-29 |
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| CN201621057749.0U Active CN206057337U (en) | 2016-09-18 | 2016-09-18 | A kind of detection means suitable for tobacco-growing soil tobacco ralstonia solanacearum |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030823A (en) * | 2018-10-15 | 2018-12-18 | 中国烟草总公司郑州烟草研究院 | A kind of colloidal gold strip and its preparation method and application detecting tobacco ralstonia solanacearum |
| CN109321462A (en) * | 2018-10-17 | 2019-02-12 | 中国烟草总公司郑州烟草研究院 | A kind of method that separation ralstonia solanacearum is combined using resistant panel screening and test strips identification in field |
| CN109406778A (en) * | 2018-10-15 | 2019-03-01 | 国家烟草质量监督检验中心 | The time-resolved fluorescence quantitative test paper item and its preparation method and application of ralstonia solanacearum in a kind of detection tobacco leaf |
| CN111487230A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Ralstonia solanacearum detection device |
| CN111487231A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ short-term test device |
| CN111487229A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ detection device based on backlight excitation principle |
-
2016
- 2016-09-18 CN CN201621057749.0U patent/CN206057337U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030823A (en) * | 2018-10-15 | 2018-12-18 | 中国烟草总公司郑州烟草研究院 | A kind of colloidal gold strip and its preparation method and application detecting tobacco ralstonia solanacearum |
| CN109406778A (en) * | 2018-10-15 | 2019-03-01 | 国家烟草质量监督检验中心 | The time-resolved fluorescence quantitative test paper item and its preparation method and application of ralstonia solanacearum in a kind of detection tobacco leaf |
| CN109030823B (en) * | 2018-10-15 | 2020-10-27 | 中国烟草总公司郑州烟草研究院 | Colloidal gold test strip for detecting ralstonia solanacearum and preparation method and application thereof |
| CN109321462A (en) * | 2018-10-17 | 2019-02-12 | 中国烟草总公司郑州烟草研究院 | A kind of method that separation ralstonia solanacearum is combined using resistant panel screening and test strips identification in field |
| CN111487230A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Ralstonia solanacearum detection device |
| CN111487231A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ short-term test device |
| CN111487229A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Plant germ detection device based on backlight excitation principle |
| CN111487231B (en) * | 2020-04-20 | 2023-08-22 | 中国农业科学院烟草研究所 | Plant germ rapid detection device |
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