CN206002479U - Nitrite detection means based on immobilization biological enzyme modified electrode - Google Patents
Nitrite detection means based on immobilization biological enzyme modified electrode Download PDFInfo
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- CN206002479U CN206002479U CN201621061296.9U CN201621061296U CN206002479U CN 206002479 U CN206002479 U CN 206002479U CN 201621061296 U CN201621061296 U CN 201621061296U CN 206002479 U CN206002479 U CN 206002479U
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- electrode
- biological enzyme
- enzyme modified
- immobilization biological
- nitrite
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
This utility model belongs to nitrite detection means technical field, is related to a kind of nitrite detection means based on immobilization biological enzyme modified electrode.Including power supply, multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, reference electrode, auxiliary electrode, electrode connector, electrochemical workstation, test pond；Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode is followed successively by silk screen printing gold electrode, multi-wall carbon nano-tube periosteum, nitrite reductase from inside to outside；It is provided with sensor, described sensor is connected with electrochemical workstation on electrode connector；There is the reduction reaction of nitrite using nitrite reductase in this utility model, change to improve electron transmission efficiency during detection, to strengthen the stability of enzyme electrode on the working electrode (s by small current/voltage under sensor record.
Description
Technical field
This utility model belongs to nitrite detection means technical field, is related to a kind of electric based on immobilization biological enzyme modification
The nitrite detection means of pole is and in particular to a kind of be based on multi-walled carbon nano-tubes immobilization biological enzyme modified electrode nitrite
Detection means.
Background technology
Nitrite is potential carcinogen, when the nitrite of human body excess intake, in acid condition it is easy to
React with intermediate product secondary amine proteolytic in body and generate nitrosamine, research shows that nitrosamine is a kind of strong cause
Cancer material, can induce digestive system canceration, and this carcinogenesis can enter fetus by Placenta Hominiss, and nitrite was to six months
Within fetus there are very strong teratogenesis.Relevant report shows, some areas are due to drinking exceeded nitrite pollution
Water, leads to the neonatal phenomenon of congenital malformation.Measuring nitrite method at present has ethylene diamine hydrochloride spectrophotography, fluorescence
Analytic process, oscilloscopic polarography and high performance liquid chromatography etc., but these methods are usually present shortcomings, and such as sample need to be located in advance
Reason, detection time length, sensitivity is not high.Therefore, also urgent at the aspect such as food safety and clinical diagnosises and environment measuring
Need research and quick, easy, the accurate detection nitrite technology of application energy.
Utility model content
In order to solve the above problems, this utility model provides a kind of nitrite based on immobilization biological enzyme modified electrode
, there is the reduction reaction of nitrite on the working electrode (s using nitrite reductase, by sensor record in detection means
Under small current/voltage change to improve electron transmission efficiency during detection, to strengthen the stability of enzyme electrode.
For achieving the above object, this utility model adopts the following technical scheme that, one kind is based on immobilization biological enzyme modification
The nitrite detection means of electrode, including power supply, multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, reference electrode, auxiliary
Help electrode, electrode connector, electrochemical workstation, test pond；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, reference electrode, auxiliary electrode pass through electrode connector
It is connected with electrochemical workstation, described electrochemical workstation is connected with data processing terminal by data line；
The slot supporting with electrode is provided with described electrode connector, described electrode connector is provided with three potteries
Pedestal；
Described power supply pass through electrochemical workstation and multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, reference electrode,
Auxiliary electrode is connected；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode is followed successively by silk screen printing gold electrode, Duo Bi from inside to outside
Carbon nano-tube film, nitrite reductase；It is provided with sensor, described sensor and electrochemical workstation phase on electrode connector
Connect；
In there being electrolyte in test pond, in electrolyte, it is placed with multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, ginseng
Than electrode and auxiliary electrode.
Further, a kind of nitrite detection means based on immobilization biological enzyme modified electrode, at described data
Reason terminal is microcomputer.
Further, a kind of nitrite detection means based on immobilization biological enzyme modified electrode, described reference electricity
Extremely silver electrode.
Further, a kind of nitrite detection means based on immobilization biological enzyme modified electrode, described auxiliary electricity
Extremely glass-carbon electrode.
A kind of nitrite detection means based on immobilization biological enzyme modified electrode that this utility model provides, assembles
Device after, when inserting electrodes into test pond, switch on power, by electrochemical workstation export unidirectional current, with nitrite
The redox reaction of nitrite can be occurred on the working electrode of reductase, sensor can record small curent change,
Thus realizing the trace detection of nitrite.
The beneficial effects of the utility model are：Improve electron transmission efficiency during detection, enhance the stability of enzyme electrode；
Detection is sensitive, response time is fast, strong antijamming capability.
Brief description
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, below will be to embodiment
Or in description of the prior art the accompanying drawing of required use be briefly described it should be apparent that, drawings in the following description are these
Some embodiments of utility model, for those of ordinary skill in the art, on the premise of not paying creative work, also
Other accompanying drawings can be obtained according to these accompanying drawings.
Fig. 1 is equipment connection structure schematic diagram of the present utility model；
Fig. 2 is the multi-walled carbon nano-tubes immobilization biological enzyme modified electrode structural representation of embodiment 1；
Fig. 3 is test pond and the electrode connector mated condition structural representation of embodiment 1；
In figure, 1- multi-walled carbon nano-tubes immobilization biological enzyme modified electrode, 2- reference electrode, 3- auxiliary electrode, 4- electrode
Adapter, 5- electrochemical workstation, 6- test pond, 7- microcomputer, 8- data line, 9- silk screen printing gold electrode, 10-
Multi-wall carbon nano-tube periosteum, 11- nitrite reductase.
Specific embodiment
Purpose, technical scheme and advantage for making this utility model embodiment are clearer, new below in conjunction with this practicality
Accompanying drawing in type embodiment, is clearly and completely described it is clear that being retouched to the technical scheme in this utility model embodiment
The embodiment stated is a part of embodiment of this utility model, rather than whole embodiments.Based on the enforcement in this utility model
Example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, broadly fall into
The scope of this utility model protection.
Embodiment 1:
A kind of nitrite detection means based on immobilization biological enzyme modified electrode, including power supply, multi-walled carbon nano-tubes
Immobilization biological enzyme modified electrode 1, reference electrode 2, auxiliary electrode 3, electrode connector 4, electrochemical workstation 5, test pond 6；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode 1, reference electrode 2, auxiliary electrode 3 are connected by electrode
Device 4 is connected with electrochemical workstation 5, and described electrochemical workstation 5 is connected with data processing terminal by data line 8
Connect；
The slot supporting with electrode is provided with described electrode connector 4, described electrode connector 4 is provided with three potteries
Porcelain pedestal；
Described power supply passes through electrochemical workstation 5 and multi-walled carbon nano-tubes immobilization biological enzyme modified electrode 1, reference electrode
2nd, auxiliary electrode 3 is connected；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode 1 is followed successively by silk screen printing gold electrode 9, many from inside to outside
Wall carbon nano-tube periosteum 10, nitrite reductase 11；It is provided with sensor, described sensor and electrochemistry on electrode connector 4
Work station is connected；
In there being electrolyte in test pond 6, be placed with electrolyte multi-walled carbon nano-tubes immobilization biological enzyme modified electrode 1,
Reference electrode 2 and auxiliary electrode 3.
Described data processing terminal is microcomputer 7.
Described reference electrode 2 is silver electrode.
Described auxiliary electrode 3 is glass-carbon electrode.
A kind of nitrite detection means based on immobilization biological enzyme modified electrode that the present embodiment provides, assembles
After device, when inserting electrodes into test pond, switch on power, unidirectional current is exported by electrochemical workstation, with nitrite also
The redox reaction of nitrite can be occurred on the working electrode of protoenzyme, sensor can record small curent change, from
And realize the trace detection of nitrite.
The having the beneficial effect that of the present embodiment：Improve electron transmission efficiency during detection, enhance the stability of enzyme electrode；Inspection
Survey is sensitive, response time is fast, strong antijamming capability.
Embodiment 2：
Described data processing terminal is microcomputer 7.
Remaining is all same as Example 1.
Silk screen printing gold electrode is polishing to light with toothpaste, kieselguhr on flannelette, and successively in acetone, 0.5mol/L
NaOH solution, 1:Ultrasound wave cleaning in 1 concentrated nitric acid and distilled water, 3～5min every time, by 10ml MWNT (multi-wall carbon nano-tube
Pipe raw material) solution Deca in electrode surface, be placed in 60 DEG C of baking ovens dry film forming after, electrode is placed in 4 DEG C of refrigerators in enzyme liquid
Overnight, rinse electrode 3 times with PBS before, wash away electrode surface unlocked on enzyme.
Prepare liquid is imported test pond, when electrode inserts test pond, switches on power, working electrode can nitrite occur
Reduction reaction, sensor can record small current/voltage change.With this method coordination electrode potential of cyclic voltammetry with
Different speed, is repeatedly scanned with triangular waveform one or many in time, and potential range is to make alternately occur not on electrode
Same reduction and oxidation reaction, and record current-potential curve, thus quantitatively determine reactant concentration.
For this utility model person of an ordinary skill in the technical field, conceive without departing from this utility model
Under the premise of, its framework form can be flexible and changeable, simply makes some simple deduction or replace, all should be considered as belonging to this practicality
The new scope of patent protection being determined by the claims submitted to.
Claims (4)
1. a kind of nitrite detection means based on immobilization biological enzyme modified electrode it is characterised in that：Including power supply, Duo Bi
CNT immobilization biological enzyme modified electrode (1), reference electrode (2), auxiliary electrode (3), electrode connector (4), electrochemistry
Work station (5), test pond (6)；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode (1), reference electrode (2), auxiliary electrode (3) are by electrode even
Connect device (4) to be connected with electrochemical workstation (5), described electrochemical workstation (5) passes through data line (8) and data processing
Terminal is connected；
The slot supporting with electrode is provided with described electrode connector (4), described electrode connector (4) is provided with three potteries
Porcelain pedestal；
Described power supply passes through electrochemical workstation (5) and multi-walled carbon nano-tubes immobilization biological enzyme modified electrode (1), reference electrode
(2), auxiliary electrode (3) is connected；
Described multi-walled carbon nano-tubes immobilization biological enzyme modified electrode (1) is followed successively by silk screen printing gold electrode (9), many from inside to outside
Wall carbon nano-tube periosteum (10), nitrite reductase (11)；Electrode connector is provided with sensor on (4), described sensor with
Electrochemical workstation is connected；
In there being electrolyte in test pond (6), be placed with electrolyte multi-walled carbon nano-tubes immobilization biological enzyme modified electrode (1),
Reference electrode (2) and auxiliary electrode (3).
2. the nitrite detection means based on immobilization biological enzyme modified electrode as claimed in claim 1 it is characterised in that：
Described data processing terminal is microcomputer (7).
3. the nitrite detection means based on immobilization biological enzyme modified electrode as claimed in claim 1 it is characterised in that：
Described reference electrode (2) is silver electrode.
4. the nitrite detection means based on immobilization biological enzyme modified electrode as claimed in claim 1 it is characterised in that：
Described auxiliary electrode (3) is glass-carbon electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621061296.9U CN206002479U (en) | 2016-09-19 | 2016-09-19 | Nitrite detection means based on immobilization biological enzyme modified electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621061296.9U CN206002479U (en) | 2016-09-19 | 2016-09-19 | Nitrite detection means based on immobilization biological enzyme modified electrode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206002479U true CN206002479U (en) | 2017-03-08 |
Family
ID=58193191
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621061296.9U Expired - Fee Related CN206002479U (en) | 2016-09-19 | 2016-09-19 | Nitrite detection means based on immobilization biological enzyme modified electrode |
Country Status (1)
| Country | Link |
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| CN (1) | CN206002479U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108572205A (en) * | 2018-04-26 | 2018-09-25 | 中国科学院合肥物质科学研究院 | The preparation method and device for fast detecting of nitrite detecting electrode and chemical oxygen consumption (COC) detecting electrode |
| CN110749641A (en) * | 2019-11-04 | 2020-02-04 | 盐城工学院 | Sensor for monitoring nitrate concentration in water body in real time |
| CN114894871A (en) * | 2022-05-16 | 2022-08-12 | 安徽大学 | Preparation method and application of high-sensitivity nitrite reductase bioelectrode |
-
2016
- 2016-09-19 CN CN201621061296.9U patent/CN206002479U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108572205A (en) * | 2018-04-26 | 2018-09-25 | 中国科学院合肥物质科学研究院 | The preparation method and device for fast detecting of nitrite detecting electrode and chemical oxygen consumption (COC) detecting electrode |
| CN108572205B (en) * | 2018-04-26 | 2020-05-08 | 中国科学院合肥物质科学研究院 | Preparation method of nitrite detection electrode and chemical oxygen demand detection electrode and rapid detection device |
| CN110749641A (en) * | 2019-11-04 | 2020-02-04 | 盐城工学院 | Sensor for monitoring nitrate concentration in water body in real time |
| CN114894871A (en) * | 2022-05-16 | 2022-08-12 | 安徽大学 | Preparation method and application of high-sensitivity nitrite reductase bioelectrode |
| CN114894871B (en) * | 2022-05-16 | 2024-01-16 | 安徽大学 | Preparation method and application of high-sensitivity nitrite reductase bioelectrode |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170308 Termination date: 20170919 |
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| CF01 | Termination of patent right due to non-payment of annual fee |