CN205941571U - Detect reaction box - Google Patents
Detect reaction box Download PDFInfo
- Publication number
- CN205941571U CN205941571U CN201620733260.4U CN201620733260U CN205941571U CN 205941571 U CN205941571 U CN 205941571U CN 201620733260 U CN201620733260 U CN 201620733260U CN 205941571 U CN205941571 U CN 205941571U
- Authority
- CN
- China
- Prior art keywords
- reagent
- reaction box
- box
- detection
- fuisw rib
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a biological sample test technical field especially relates to a detect reaction box, including installing reagent memory and the sampling rod in reaction box, be equipped with reagent release position on the reagent memory, reaction box includes towards the wallboard of reagent release position, be equipped with the fuisw rib on the wallboard, the fuisw rib releases the liquid drop contact on the end of position with reagent, and the fuisw rib is used for the liquid drop on the end of water conservancy diversion reagent release position. Set up the fuisw rib on this practical reaction box, in the time of in liquid reagent releases reaction box from the reagent memory, through fuisw rib and the terminal liquid drop contact in reagent release position, made the liquid drop leave reagent release position along the drainage direction of fuisw rib, avoided the part of reagent liquid drop to remain, guaranteed the precision of reagent volume control, made the reaction more abundant.
Description
Technical field
This utility model is related to detection of biological samples technical field, more particularly, to a kind of detection reaction box.
Background technology
Medical science in-vitro diagnosis play the part of the role of suitable reuse, the measurement that it can be qualitative or quantitative in medical industry now
The change of various Biological indicators in human fluid, to provide medical diagnosis on disease or the treatment consulting such as index, for example, saccharifying in blood
The detection of hemoglobin (HbA1c) is most important for the diagnosis of diabetes and control.
Glycolated hemoglobin is the product that in blood of human body, endoerythrocytic hemoglobin is combined with blood glucose, when Portugal in blood
When grape sugar concentration is higher, the content of the HbA1c that human body is formed also can be of a relatively high.In human body, the average life of erythrocyte is 120
My god, before cell death, in blood, the content of HbA1c also can remain relatively unchanged over, and therefore, glycolated hemoglobin test is generally
Patient's glycemic control situation of nearly 8~12 weeks can be reflected, do not affected by once in a while blood glucose rise or reduction.
Also multiple concentration being designed for detection and analysis thing are had, such as in prior art:
As shown in figure 1, US1562237 discloses a kind of reaction box, reaction box includes reaction channel and liquid reagent storage
Portion, reaction channel is provided with dry reagent, and liquid reagent storage part is used for depositing buffer or other liquid reagent, and liquid tries
Agent storage part includes storing body 30', and storage body is sealed by sealing member or thin film 32', and thin film 32' has a far-end 33',
Directly tearing thin film from far-end makes thin film separate with storage body, and the reagent in storage body is discharged in reaction channel.
When discharging liquid reagent, the thin film 32' artificially script being sealed on storage body 30' is needed to separate, then
Thin film is removed.Although aforesaid way can tear thin film, discharge liquid reagent, by the far-end 33' of one section of extension from
Tear thin film and be extremely difficult on storage body 30', overexertion or firmly kick the beam and easily cause thin film and break when such as artificially tearing
Open or tear off not exclusively, so that liquid reagent can not be discharged completely, during detection, liquid reagent is very few, cause the inclined of testing result
Difference;On the other hand, in the non-detection period, thin film 32' is exposed aerial, easily occurs artificially or other factorses are ripped or broken
Bad risk.
As shown in Fig. 2 US5272093 discloses a kind of " reagent container and its delivering method ", including reagent storage chamber 12'
With the sealing member 40' being sealed in reagent storage chamber, sealing member is folding two-layer setting, and one layer is used for sealed reagent storage
Chamber, another layer extends to and forms extension 42' outside reagent storage chamber, and extension 42' is used for tearing in release reagent storage chamber
Reagent.When discharging liquid reagent, the sealing member 40' artificially script being sealed in reagent storage chamber 12' is needed to separate,
If overexertion or firmly kick the beam is easy to cause thin film to disconnect or tears off not exclusively, cause liquid reagent complete
Release, makes liquid reagent during detection very few, causes the deviation of testing result.
As shown in figure 3, US8846380 discloses a kind of " for detecting the reaction box of glycolated hemoglobin concentration ", reaction
Box include for accommodate test kit blood sample the firstth area, for accommodating the secondth area, detection zone and the reagent of wash solution
Bag, the reagent in reagent bag and wash solution are stored separately, and are sealed up for safekeeping with aluminium foil 120'.When in reagent bag insertion reaction box,
Aluminium foil is torn by reaction box, and the reagent in reagent bag and wash solution are temporarily stored in the first reaction zone of reaction box and second anti-respectively
Answer in area, reacted with blood sample successively by the rotation of reaction box, thus solving storage and the assignment problem of reagent.
Above-mentioned reaction box, in detection, firstly, it is necessary to artificially fold the release portion 130' on aluminium foil, makes 130' pair, release portion
Clamper in quasi- detection box, then by the reagent bag insertion detection box after be aligned so that detection box can excise release portion, realize
Aluminium foil is separated with reagent bag.This design is relative complex, and operating procedure is various, and, artificial alignment, easily is needed in grafting
Grafting situation not in place occurs and causes grafting repeatedly.On the other hand, reaction box and reagent bag adopt split-type design, reaction
The opened type design of box, if misoperation, easily falls into foreign body in reaction box, impacts the accuracy of testing result.Again
Person, in the non-detection period, release portion 130' is exposed aerial, the wind that artificial or other factorses are ripped or destroyed easily
Danger.
Reaction box of the prior art includes sampling needle, reagent storing device, reacting part, detection zone etc..Reaction box is in detection
During reaction, the reagent in reagent storing device is released to reacting part and participates in detection reaction, is discharged into the process of reacting part in reagent
In, due to reaction box small volume, compact conformation, the distance between reagent storing device and wallboard of reaction box are relatively near, wallboard with
It is also easy to produce the absorption affinity to reagent liquid between the reagent liberation port of reagent storing device, make reagent portion remain in reagent liberation port
In the gap of wallboard.In addition, in order that reagent more smoothly enters reacting part, also can be in reagent storing device reagent liberation port
Position Design drain most advanced and sophisticated, make reagent by drain tip water conservancy diversion after reagent liberation port, finally enter under gravity
Enter reacting part, although drain most advanced and sophisticated can more preferable water conservancy diversion, during reagent release, drain is most advanced and sophisticated easily to have extension liquid phenomenon,
Reaction reagent part is made to remain on drain tip, meanwhile, drain is most advanced and sophisticated close together with wallboard, there is also portion of reagent quilt
Absorption is between drain tip and wallboard.The part residual of reaction reagent, makes reaction not abundant, causes testing result precision
Reduction.
Content of the invention
Technical problem to be solved in the utility model is for the above-mentioned problems in the prior art, there is provided a kind of
Detection reaction box.
For solving the above problems, the technical solution of the utility model is:
A kind of detection reaction box, including the reagent storing device being arranged in reaction box and sampling rod, reagent storing device sets
Have reagent release position, described reaction box includes the wallboard towards reagent release position, described wallboard is provided with fuisw rib, fuisw rib with
Drop contact on reagent release position end, fuisw rib is used for the drop on water conservancy diversion reagent release position end.
Preferably, described fuisw rib is contacted with reagent release position end.
Preferably, described reaction box also includes reacting part, and fuisw rib reaches in reacting part.
Preferably, described reaction box includes side plate, and described fuisw rib is with side plate at an angle.
Preferably, described fuisw rib is domed.
Preferably, described fuisw rib includes the guide face towards reagent release position, and described guide face is smooth curved surface.
Preferably, described fuisw rib also includes two adjacent with guide face sidepiece guide face, two sidepiece guide faces with
The junction of wallboard is smooth curved surface.
Preferably, described reagent release position includes reagent liberation port.
Preferably, described reagent release position also includes being connected to the deflector below reagent liberation port.
Preferably, described sampling rod is arranged on inside reaction box.
Preferably, described reaction box is provided with installation passage, and described sampling rod is connected in installation passage.
It is compared to prior art, the beneficial effects of the utility model are:
It is provided with fuisw rib on reaction box of the present utility model, be discharged into reaction box from reagent storing device in liquid reagent
When middle, by the drop contact of fuisw rib and reagent release position end, drop is made to leave reagent along the drain direction of fuisw rib
Release position, it is to avoid the Local residues of reagent droplet, it is ensured that the precision that controls of reagent volumes, make the reaction more abundant.
Brief description
Fig. 1 is the structural representation of reaction box in prior art.
Fig. 2 is the structural representation of reagent container in prior art.
Fig. 3 is the partial structural diagram of reaction box in prior art.
Fig. 4 is the axonometric schematic diagram of partial structurtes in detecting system.
Fig. 5 be detection reaction box in panel hide after structural representation.
Fig. 6 is the structural representation after detection reaction box dorsulum is hidden.
Fig. 7 is the attachment structure schematic diagram of reagent storage part, sealing member and reagent release portion.
Fig. 7 A is the structural representation after sealing member is torn in Fig. 7.
Fig. 8 is the structural representation of reagent storage part.
Fig. 8 A is the structural representation of one angle of reagent storage part.
Fig. 8 B is the structural representation of reagent another angle of storage part.
Fig. 9 is a kind of structural representation of embodiment of reagent storage part.
Figure 10 is the structural representation of reagent storage part another embodiment.
Figure 11 is the attachment structure schematic diagram in upper lid, reagent storage part and reagent release portion in detection reaction box.
Figure 12 is the attachment structure schematic diagram of box body and push rod in detection reaction box.
Figure 12 A is to detect that in reaction box, push rod is relative to the structural representation after box body slip.
Figure 13 is the structural representation that in detection reaction box, box body is connected another embodiment with push rod.
Figure 14 is the axle geodesic structure schematic diagram in release portion in detection reaction box.
Figure 15 is the mplifying structure schematic diagram at A in Fig. 6.
Figure 16 is the axle geodesic structure schematic diagram that in detection reaction box, box body is connected with push rod.
Figure 16 A is the mplifying structure schematic diagram at C in Figure 16.
Figure 17 is the axle geodesic structure schematic diagram of the another embodiment that box body is connected with push rod in detection reaction box.
Figure 18 is the mplifying structure schematic diagram at D in Fig. 5.
Figure 19 is the axonometric schematic diagram of detection reaction box external structure.
Figure 19 A is the backsight structural representation of detection reaction box.
Figure 20 is the structural representation of upper lid in detection reaction box.
Figure 20 A is the mplifying structure schematic diagram at B in Figure 20.
Figure 20 B is another angled arrangement schematic diagram of upper lid in detection reaction box.
Figure 21 is the attachment structure schematic diagram of upper lid and reagent storage part in detection reaction box.
Figure 22 is the explosive view of detection reaction box.
Figure 23 is the cross-sectional view of detection box.
Figure 24 is the structural representation of internal part in detection box.
Figure 25 is that detection reaction box insertion detects that in box, push rod is by the cross-sectional view of push rod jack-up.
Figure 26 is the structural representation of elastic component in detection box.
Figure 27 is the structural representation of shell fragment in detection box.
Figure 28 is the reagent view being in step 0 during detection reaction box detection.
Figure 28 A-28F is the reagent view being in step 1-6 during detection reaction box detection respectively.
Figure 29 is the structural representation of fuisw rib on detection reaction box.
Figure 30 is the flow-guiding structure schematic diagram after backboard perspective on detection reaction box.
Figure 30 A is the structural representation in H-H section for the Figure 30.
Figure 30 B is the mplifying structure schematic diagram at E in Figure 30 A.
Figure 31 is a kind of structural representation of embodiment of fuisw rib in detection reaction box.
Figure 32 is the structural representation of the another embodiment of fuisw rib in detection reaction box.
Figure 33 is the structural representation of the another embodiment of fuisw rib in detection reaction box.
Figure 34 is a kind of structural representation of embodiment of fuisw rib and reagent storing device in detection reaction box.
Specific embodiment
Further describe this utility model with reference to the accompanying drawings and examples, but protection domain of the present utility model is simultaneously
Not limited to this.
As shown in figure 4, detecting system of the present utility model is used for the concentration of analyte in quick detection biological sample, bag
Include detection reaction box 100 and external equipment, external equipment is the equipment in addition to detection reaction box 100 body, and wherein, outside sets
Standby also referred to as testing equipment, external equipment or testing equipment are provided with a detection box 200, detection reaction box 100 and detection box 200
Support the use.Detection reaction box 100 is as the container of reaction, it is possible to achieve the storage of plurality of reagents, the order of plurality of reagents add
Plus and the function such as devil liquor recovery.Detection box 200 is used for placing detection reaction box 100, and detection box 200 is arranged on testing equipment,
And provide detection reaction box 100 to rotate by testing equipment, detect that the machinery of reagent mixing in reaction box 100, reagent release etc. moves
Power and control, are additionally provided with optics simultaneously on external equipment, optics is used for detecting the concentration of detectable substance in fluid sample.
Wherein, detection reaction box is hereinafter referred to as reaction box.
As shown in Figure 5 and Figure 6, it is provided with reagent storage part 102, reagent release portion 150, reacting part and inspection in reaction box 100
Survey area 28.Reagent storage part 102 and reagent release portion 150 are all encapsulated in reaction box 100.Preferably, in described reaction box 100
It is provided with a cavity, described reagent storage part 102 is housed in cavity.Reagent storage part 120 is encapsulated in reaction box for individual components
In 100, be conducive to the quick assembling of reaction box.Reagent storage part 102 is used for sealed type storage solid particle, powder or liquid examination
Agent, reagent release portion 150 detects in testing equipment and can quickly open reagent storage part 102 release examination under the cooperation of box 200
Agent is in the reacting part of reaction box 100.Reacting part be used for biological sample and reagent temporary, mix and reacts, and pass through to detect
Area 28 is detected to the intermediate product of reaction or final product.
As shown in Figure 7 and Figure 8, reagent storage part 102 includes at least one reagent accommodating chamber, and reagent storage part 102 is by close
Sealing 104 seals, and that is, reagent accommodating chamber is sealed by sealing member 104.Reagent storage part 102 is also referred to as reagent storing device, and reagent is deposited
Storage portion 102 includes multiple reagent accommodating chambers, and multiple reagent accommodating chambers become array distribution.The quantity of reagent accommodating chamber is anti-according to detection
Should required for reagent type, the order added set, such as one, two or multiple, reagent accommodating chamber each other it
Between be independent, that is, multiple reagent accommodating chambers are provided with spacing each other, become array distribution.For example, two reagent accommodating chambers
Can be laterally to become row pattern or longitudinally become column-shaped, can also be to angular distribution;For another example, multiple reagent accommodating chambers are permissible
Interval plug hole distribution.The capacity of reagent accommodating chamber is also that the dosage according to required for detection reaction is set.
As one kind preferably, described reagent storage part includes at least two row reagent accommodating chambers, and each column includes at least one examination
Agent accommodating chamber.Preferably, as shown in figure 8, reagent storage part 102 includes two reagent accommodating chambers, about two reagent accommodating chambers
Transversely arranged.I.e. reagent accommodating chamber 106 and reagent accommodating chamber 108 are in the left and right distribution of a row two row, and the bottom of reagent accommodating chamber is big
Cause in the same horizontal line.Therefore, the reagent when tearing sealing member 104, in reagent accommodating chamber 106 and reagent accommodating chamber 108
Can together be discharged in reaction box 100.
As shown in figure 9, as a kind of embodiment of reagent storage part 102, the feelings of two reagent accommodating chamber longitudinal arrangements
Shape, that is, reagent accommodating chamber 110 and reagent accommodating chamber 112 be distributed up and down in two row's string, in partially torn sealing member 104, position
Reagent accommodating chamber 112 in lower row preferentially discharges reagent, according to the requirement in response time, continues to tear sealing member 104, makes to be located at
The reagent accommodating chamber 110 release reagent of upper row.It is possible to sequentially add reagent under the degree controlling sealing member 104 to tear, control
The carrying out of system reaction.
As shown in Figure 10, as the another embodiment of reagent storage part, four reagent accommodating chambers are in array arrangement
Situation, that is, reagent accommodating chamber 114, reagent accommodating chamber 116, reagent accommodating chamber 118 and reagent accommodating chamber 120 are divided in two row four column-shaped
Cloth.Under the degree controlling sealing member 104 to tear, the reagent in the reagent accommodating chamber 118 and reagent accommodating chamber 120 of lower row is preferential
Release, the reagent then arranged in release.
As one kind preferably, described reagent storing device includes the examination for depositing solid particle reagent or powder particle reagent
Agent accommodating chamber.Solid particle reagent or powder particle reagent is sealed in reagent storing device, it is to avoid reagent is in reaction box
Arbitrarily move, it is to avoid the problem needing in prior art dried reagent is fixed, meanwhile, under sealing state, also improve
Effect duration storage life under this reagent room temperature, so as to be distinguished with other reagent, is compared under particularly suitable room temperature and is difficult to ensure and deposits
Reagent.
Preferably, described solid particle reagent or powder particle reagent are lyophilized solid particle reagents or freeze-dried powder granule
Reagent.Preferably, described solid particle reagent is latex lyophilizing ball.Latex lyophilizing ball, on the one hand achieves anti-to latex antibody
Answer the maximization of active protection, on the other hand, greatly improve reagent room temperature storage life of lower effect duration.
As one kind preferably, described reagent storing device also includes the reagent accommodating chamber for depositing liquid reagent.Reagent holds
Receiving chamber can be used for depositing solid particle, powder particle or liquid reagent, can be set according to detection needs.
As one kind preferably, as shown in Figure 8 A, at least one reagent accommodating chamber is provided with injection orifice 130, and injection orifice 130 is even
Logical reagent accommodating chamber and space outerpace, reagent accommodating chamber and injection orifice 130 are sealed by sealing member 104.Preferably, injection orifice
130 located at the reagent accommodating chamber for depositing powder reagent or liquid reagent.For eased sealing liquid reagent or
Powder reagent, in sealing, first use sealing member 104 sealed reagent accommodating chamber and by exposed for injection orifice 130 outside, then by liquid
Or powder reagent is injected in reagent accommodating chamber, then sealing injection hole 130 again, the effect of sealing be ensure that with this, and reduce
Sealing liquid or the difficulty of powder reagent.Reagent accommodating chamber and injection orifice can be sealed by same sealing member timesharing substep, can be by
Two sealing members seal respectively, such as using sealing plug sealing injection hole etc..
As a kind of preferred, as shown in Figure 8 B, the back of reagent storage part 102 is additionally provided with the chamber for installing desiccant
Body 132.When reagent storage part 102 is arranged in reaction box 100, with cavity 132 and panel 10 inwall, desiccant is installed
In cavity 132.
As one kind preferably, reagent storage part 102 includes two reagent accommodating chambers, and two reagent accommodating chambers are respectively used to deposit
Storage solid particle reagent and liquid reagent.The present embodiment is included as a example two reagent accommodating chambers 106,108 by reagent storage part 102
It is described in detail, reagent accommodating chamber 106 storage solid particle reagent and reagent accommodating chamber 108 store liquid reagent, and in examination
The upper end of agent accommodating chamber 108 is provided with injection orifice 130.First solid particle reagent is placed in reagent accommodating chamber 106 in sealing,
With the cavity hatch of sealing member 104 sealed reagent accommodating chamber 106 and reagent accommodating chamber 108, then liquid reagent is injected into reagent
In accommodating chamber 108, followed by sealing member 104 sealing injection hole 130.
As one kind preferably, the bottom design of reagent accommodating chamber takes the form of inclined plane.When discharging reagent, reagent is facilitated quickly to flow
Go out, decrease as far as possible reagent accommodate intracavity residual it is ensured that reagent volumes control precision.
As shown in fig. 7, reagent storage part 102 is sealed by sealing member 104, will reagent be sealed in reagent accommodating chamber, close
Sealing 104, in the presence of external force, can be torn off with reagent accommodating chamber, sealing member 104 tear off can be by from left to right, by under
Toward first-class mode, so that sealing member tears off from lower to upper as a example illustrate herein, as shown in Figure 7 A.Sealing member 104 can be aluminum
Conventional use of sealing member in the prior arts such as paper tinsel, thin film.
As shown in Figure 29,30,30A and 30B, described reaction box includes reagent storing device 102 He being arranged in reaction box
Sampling rod, described reagent storing device 102 is provided with reagent release position, and reaction box 100 includes the wallboard towards reagent release position, institute
State wallboard and be provided with fuisw rib 70, the drop contact on fuisw rib 70 and reagent release position end, fuisw rib 70 is used for water conservancy diversion to be tried
Drop on agent release position end.Described reagent release position refers to that liquid reagent flows out to reagent from reagent storing device and leaves examination
The position of agent memorizer, i.e. fluid passage on reagent storing device for the liquid reagent.Corresponding, reagent release position end refers to
The tail end of fluid passage.Described wallboard is backboard 12 or division board 36 on reaction box 100 mentioned below, when fuisw rib 70 is pacified
When being contained in backboard 12, reagent release portion adopts manually mode of the prior art, when fuisw rib 70 is arranged on division board 36
When, reagent release portion adopts automated manner herein.As shown in figure 5, described reaction box 100 is provided with for installing sampling rod 30
Installation passage, in the installation passage of sampling rod 30 insertion reaction box 100;Sampling rod 30 still can be by sample in the form of dropper
Directly drip to reaction box outside reaction box.When liquid reagent is discharged in reaction box from reagent storing device, partial drop
Easily remain on reagent release position end or absorption is between reagent release position end and wallboard, released with reagent by fuisw rib
Put the drop contact of an end, make drop leave reagent release position along the drain direction of fuisw rib, it is to avoid reagent droplet
Local residues, it is ensured that the precision of reagent volumes control, make reaction more abundant.
In embodiment as shown in figure 34, described reagent release position includes reagent liberation port 140.Reagent liberation port 140
Bottom be reagent release position end.In embodiment as shown in Figure 30 33, described reagent release position also includes connecting
Deflector 142 below reagent liberation port 140.Deflector 142 be used for liquid water conservancy diversion, make liquid reagent more smoothly from
Open reagent storing device 102, deflector 142 is in tip-angled shape, the bottom of deflector 142 is reagent release position end.Preferably,
Described fuisw rib 70 is contacted with reagent release position end.The drop of reagent release position end is made to increase with the contact surface of fuisw rib 70
Plus, more quick drain drop.As shown in figure 30b, described fuisw rib includes the contact jaw 72 being sequentially connected and leading end 74,
Described contact jaw 72 contacts with the drop contact on reagent release position end or with reagent release position end, described leading end 74 by
Contact jaw 72 extends downwardly.Preferably, described fuisw rib is in fine strip shape.Contact jaw 72 is used for contacting drop and by drop drain
To on leading end 74, leading end 74 is used for the drop on contact jaw 72 is directed to the specified location of reaction box 100.Described guiding
The area holding 74 cross sections is sequentially reduced from top to bottom, and the bottom of leading end tapers off to a point, and is conducive to the drain of drop, as far as possible
The extension liquid phenomenon reducing leading end bottom.Contact jaw and drop contact, can quick drainage drop.Contact jaw is closer to reagent
Release position end, contact jaw is bigger with the contact surface of drop, and the drainage of fuisw rib is more obvious.
As shown in Figure 31 33, described reaction box 100 also includes reacting part, and fuisw rib 70 reaches in reacting part.I.e. fuisw rib
70 leading end 74 extends in reacting part, and contacts with the liquid reagent in reacting part.The extending design of fuisw rib 70, makes liquid
Drip and can fast and accurately be directed in reacting part.Meanwhile, the big drop being attached to reagent release position end is through fuisw rib drain
Afterwards, it is understood that there may be minimum liquid point is suspended on fuisw rib leading end bottom, the liquid in drop leading end hanging and reacting part
It is pulled away after the contact of body reagent, it is to avoid residual on fuisw rib for the droplet.In embodiment as shown in figure 32, fuisw rib
70 contact jaw 72 upwardly extends.Be conducive to quickly guiding the liquid of reagent storing device to enter reacting part.
In embodiment as shown in Figure 29 34, described reaction box 100 includes side plate, described fuisw rib 70 and side plate
At an angle.Fuisw rib 70 is obliquely installed or is be arranged in parallel with side plate.Described side plate is reaction box 100 mentioned below
On left plate 6 or right plate 8.Preferably, in the water conservancy diversion direction of described fuisw rib 70 and reagent storing device liquid flowing side
To consistent.When agent reservoirs discharge the flow direction of liquid reagent with horizontal plane, fuisw rib vertical level is arranged;
The agent reservoirs of reaction box when discharging reagent, reaction box horizontal by the inclination of certain angle, now liquid reagent
Flow direction becomes angle of inclination to discharge with side plate, and fuisw rib is obliquely installed, and the incline direction of fuisw rib is in reagent storing device
The flow direction of liquid.The water conservancy diversion direction of fuisw rib is consistent with the flow direction of liquid in reagent storing device, is conducive to reduction to draw
Resistance during stream, makes reagent quickly flow down.
Preferably, described fuisw rib is domed.Preferably, the longitudinal section triangular shape of described fuisw rib.Described three
Angular for rounded triangle.Described triangle is selected from:Right angled triangle, obtuse triangle or acute triangle.As shown in figure 33
Embodiment in, the rectangular triangle in longitudinal section of described fuisw rib 70, one of right-angle side of right angled triangle is solid
It is located on wallboard, fuisw rib 70 can be contact jaw 72 with the part of drop contact, part below 72 for the contact jaw is leading end
74.In embodiment as shown in Figure 30 A, 31 and 32, the longitudinal section triangle in obtuse angle of described fuisw rib 70, obtuse angle triangle
The maximum hypotenuse of shape is installed on wallboard, on fuisw rib 70 can with the part of drop contact be contact jaw 72, contact jaw 72 with
Under part be leading end 74, the part of contact jaw more than 72 is the extension of contact jaw 72.
As shown in figure 30b, described fuisw rib 70 includes installed surface 80 guide face relative with installed surface 82 and and installed surface
Two adjacent sidepiece guide faces 84, described installed surface 80 is installed on wallboard.Guide face 82 is arranged towards reagent release position, institute
Stating guide face 82 is smooth curved surface, and the junction of two sidepiece guide faces 84 and wallboard is smooth curved surface.The design of smooth curved surface,
Make that the drain of fuisw rib is more smooth, resistance is less.
As is illustrated by figs. 11 and 12, reagent release portion 150 includes push rod 152, and push rod 152 is connected with sealing member 104, described
Push rod 152 is used for cooperating with external equipment making sealing member separate with reagent storage part.As shown in Figure 7 A, i.e. sealing member 104
Paste or be fixed on push rod 152, when external device acts on push rod 152, push rod 152 is coordinated with the inwall of reaction box 100
Produce the motion with respect to reagent storage part 102, while push rod 152 moves, carry dynamic seal 104 relative reagent storage part
102 motions, the action that is, sealing member 104 tears off is so that the reagent in reagent accommodating chamber is discharged in reaction box 100.Push rod 152
The motion of relative reagent storage part 102 can be turn left by from left to right, by the right side, from lower to upper etc. mode realize.
Preferred as one kind, as shown in fig. 7, one end sealed reagent storage part 102 of sealing member 104, after the other end folds
It is pasted onto on push rod 152.
As one kind preferably, described push rod 152 is provided with the forced section cooperating with external equipment.Forced section is used for holding
The thrust of external equipment offer is provided.
As one kind preferably, push rod 152 is all housed in inside reaction box 100, and the thrust piece of external equipment needs to stretch into
Or act on the forced section of push rod 152 to inside reaction box 100 by other means.Push rod 152 is all housed in reaction box,
Human contact is less than the push rod in reaction box, artificially free-hand nor promote push rod 152 action, only after by external tool
Push rod 152 action just can be made.So avoid in the non-detection period, maloperation or other reason are artificially torn or breakseal
Part 104 causes the possibility that reagent leaks in advance.
As a kind of preferred, as shown in figure 13, in reaction box 100, it is provided with chute 38.On the one hand, chute 38 is by push rod 152
It is embedded in slideway, on the other hand, chute 38 is slidably matched with push rod 152.As an embodiment, chute 38 is arranged on backboard
On 12, chute 38 can be designed as only backboard 12 and two trough rims composition, and now, 152 liang of lateral sections of push rod are housed in chute
In 38.
As one kind preferably, as shown in Figure 12 and Figure 12 A, described chute 38 is provided with division board 36, and division board 36 is isolated
Chute 38 and the inner space of reaction box 100.As a kind of embodiment, chute 38 is the column of at least one end open, post
By part backboard 12,38 liang of sides of chute and division board 36 forms that is to say, that chute 38 is tightly connected for four sides of shape body
Division board 36, division board 36 separates the inner space of chute 38 and reaction box 100, and division board 36 height will not be flowed to reactant liquor
It is design requirement outside reaction box.Chute 38 is designed to an independent region, and push rod 152 or external thrust part are isolated with reagent
Open, push rod 152 or external thrust part can be avoided to take away portion of reagent with the contact of reaction reagent.
As one kind preferably, as shown in figure 14, at least one side of described push rod 152 is provided with least one limit convex
Play 154.Spacing preiection 154 can make the more stable inside being arranged on reaction box 100 of push rod 152.Preferably, spacing preiection
154 quantity is two, is separately positioned on the left and right side of push rod 152.Preferably, described spacing preiection 154 is arranged on and pushes away
The top of bar 152 side.When push rod 152 is arranged in reaction box 100, the spacing preiection 154 on push rod 152 is in reaction box
Under 100 extruding, make push rod 152 produce elastic deformation, increased the frictional force of push rod 152 and reaction box 100, make push rod 152
Can be stable be arranged in reaction box 100, also increases resistance during push rod 152 motion.
As one kind preferably, as shown in figure 14, described push rod 152 is provided with the cell body of at least one hollow out near edge
156, described spacing preiection 154 is arranged on the lateral wall of cell body 156, and spacing preiection 154 is arranged in pairs with cell body 156.As
One specific embodiment, spacing preiection 154 is two, and two spacing preiections 154 are separately positioned on the left and right side of push rod 152
On, corresponding, the cell body 156 of hollow out is also two, is separately positioned on the left of push rod and right side is near edge, that is, left
The lateral wall of side cell body 156 is the left surface of push rod 152, and the lateral wall of right edge cell body 156 is the right flank of push rod 152,
That is, spacing preiection 154 is arranged on the lateral wall of cell body 156.The setting of hollow out cell body 156, when making push rod 152 action
More it is also easy to produce deformation, it is to avoid because rubbing excessive and to cause push rod 152 to slide dumb.
If push rod 152 is chronically at deformed state and is easy for losing certain elasticity, and makes fastening effect not good, for solving
The problems referred to above, as one kind preferably, as shown in figure 15, reaction box 100 is provided with the limited impression with spacing preiection 154 cooperation
158.Preferably, chute 38 is provided with the limited impression 158 engaging with described spacing preiection 154, the quantity of limited impression 158 with
Spacing preiection 154 is identical.When push rod 152 is under original state is normality, limited impression 158 is engaged with spacing preiection 154, this
When, push rod 152 does not deform upon;When the promotion lower slider in external force for the push rod 152, push rod 152 produces at spacing preiection 154
Deformation simultaneously leaves limited impression 158.
As one kind preferably, as shown in figure 14, described push rod is provided with a groove 162.Groove 162 is arranged on push rod 152
Upper end, groove 162 be paste groove, paste groove be used for adhesive seal part 104.Before non-adhesive seal part, the bottom land of groove 162
Place plane is slightly below groove 162 notch place plane, and after adhesive seal part 104, sealing member 104 fills and leads up groove 162, makes to push away
Bar plane is concordant.When avoiding push rod 152 to promote, cause the possibility peeled off because sealing member 104 exceeds push rod 152 surface.
As one kind preferably, the forced section of push rod 152 is bottom surface or the back of push rod, when forced section is the bottom of push rod
When, external force acts on the bottom surface of push rod, makes push rod 152 tear sealing member 104 from lower to upper, when forced section is the back of push rod
When, external force acts on the back of push rod, equally promotes push rod 152 to tear sealing member 104 from lower to upper.The forced section of push rod 152
Can also be upper bottom surface, left surface or the right flank of push rod, when being upper bottom surface, push rod can be pulled from top;For left surface
When, push rod can be promoted from left to right;During for right flank, can turn left promotion push rod from the right side.
As another embodiment, the forced section of push rod 152 is connected with extraneous thrust piece magnetic, i.e. being subject to of push rod 152
Power portion, extraneous thrust piece are the magnet assembly attracting each other, such as iron block, Magnet etc..Push rod 152 and extraneous thrust piece are by reaction box
100 isolation, under magnetic action, drive push rod 152 action by extraneous thrust piece.
As one kind preferably, reaction box 100 is provided with an opening, and forced section is exposed in said opening, and forced section passes through
Opening receives external thrust.I.e. external thrust part can stretch in opening, contact and promote with the push rod 152 in reaction box 100
Its action, aperture position arranges related to the forced section setting of push rod 152.
As a kind of embodiment, as shown in figure 16, the forced section of push rod 152 is the bottom surface of push rod, aforesaid opening 34
It is arranged on the base plate 4 of reaction box 100.Preferably, as shown in Figure 16 A, opening 34 is provided with chamfering 40, and push rod 152 is all received
Holding has certain interval, to avoid collision during maloperation in chute 38 and apart from opening 34.External thrust part is by opening 34
Chamfering 40 at introduce and stretch into chute 38, contact with push rod 152, and promote push rod 152 and external thrust part sliding with chute 38
Dynamic cooperation.
As another embodiment, as shown in figure 17, the forced section of push rod 152 is the back of push rod 152, aforementioned opening
34 are arranged on the backboard 12 of reaction box 100.Preferably, the back of push rod 152 is provided with fin or recess 160, fin or
Recess 160 be forced section, fin or recess 160 exposed in opening 34.External thrust part is contacted with fin or recess 160, and
Push rod 152 is promoted to be slidably matched with chute 38.
Described reacting part includes at least one reaction zone, and reaction zone receives the reagent of reagent storage part 102 release.Reacting part
Including multiple reaction zones, the quantity of reaction zone and position set according to the quantity of reagent accommodating chamber in reagent storage part 102 and anti-
Answer step related, the two kinds of reagent such as simultaneously discharging can be temporarily stored in a reaction zone it is also possible to be temporarily stored in respectively two each
In independent reaction zone;If also having in reaction zone and drying reagent, also other reaction zones can be separately provided, phase between each reaction zone
Intercommunicated, the mixing of reagent in each reaction zone can be realized by revolving reaction box.
As shown in Figure 5 and Figure 6, it is provided with detection zone 28 in reacting part, detection zone 28 can be arranged on appointing of reaction box 100
On the circulation passage of meaning reagent, detection zone 28 typically adopts transparent material, so that transmitted light that optics sends or scattered light is entered
Enter reaction box 100.It is provided with conducting element, more specifically, conducting element is arranged on reagent between reagent storage part 102 and reacting part
Between accommodating chamber and corresponding reaction zone, conducting element makes the reagent that reagent accommodates intracavity fast and accurately be discharged in reaction zone.
As one kind preferably, as shown in Fig. 5 and Figure 18, reacting part includes at least one reaction zone, and wherein at least one is anti-
Area is answered to be the first reaction zone 26, described first reaction zone 26 is used for keeping in solid particle reagent, the first reaction zone 26 includes supporting
Portion and stop part, form a gap 47 between supporting part and stop part, the Breadth Maximum in gap 47 is less than solid particle reagent
Minimum widith.Gap between supporting part and stop part is used for stopping that the solid particle reagent in the first reaction zone enters other instead
Answer area, make what solid particle reagent can be stable to be temporarily stored on supporting part.As a kind of embodiment, supporting part is a step
46, stop part is a baffle plate, is designated as first baffle 48.First baffle 48 is vertical baffle plate, the bottom of first baffle 48 with
Support part formation gap 47.Preferably, described solid particle reagent is latex lyophilizing ball agent.The minimum width of latex lyophilizing ball agent
Degree is the Breadth Maximum with diameter greater than gap 47 of latex lyophilizing ball, so that latex lyophilizing ball agent is stopped by gap 47.
As one kind preferably, as shown in Fig. 5 and Figure 18, the first reaction zone 26 also includes second baffle 50, second baffle 50
It is obliquely installed, second baffle 50 and stop part form the second gap 49, the minimum widith in the second gap 49 is more than solid particle to be tried
The Breadth Maximum of agent.Second baffle 50 is used for solid particle reagent drain with stop part, enables solid particle reagent smoothly
Enter in the first reaction zone 26.As a specific embodiment, the Breadth Maximum of latex lyophilizing ball agent is latex lyophilizing
The diameter of ball is less than the minimum widith in the second gap 49, makes in the convenient entrance of latex lyophilizing ball agent the first reaction zone 26.
As one kind preferably, first baffle 48 and second baffle 50 are the baffle plate with radian.On the one hand make solid particle
Reagent can rapidly enter the difference it is contemplated that on Solids Particle Diameter, it is to avoid solid particle is stuck in first baffle 48 and second
Cannot fall on step 46 between baffle plate 50.
As one kind preferably, described reacting part also includes second reaction zone, and second reaction zone is used for keeping in liquid reagent.Excellent
Selection of land, second reaction zone is provided with conducting element, and conducting element includes the first drainage plate and the second drainage plate.
As a kind of specific embodiment, as shown in Figure 5 and Figure 6, reacting part includes the first reaction zone 26 and the second reaction
Area 25, the first reaction zone 26 is used for receiving the solid particle of reagent accommodating chamber 106 release, and second reaction zone 25 is used for receiving reagent
The liquid reagent of accommodating chamber 108 release.Second reaction zone 25 is provided with conducting element, and conducting element includes the first drainage plate 42 and second
Drainage plate 44, makes liquid reagent flow rapidly in second reaction zone 25.First reaction zone 26 includes the step for keeping in reagent
46th, first baffle 48 and second baffle 50, first baffle 48 and second baffle 50 are used for guiding solid particle to enter step 46, with
When, form gap 47 between first baffle 48 and step 46, this gap 47 can stop solid particle from entering second reaction zone 25,
And allow liquid reagent to flow in the first reaction zone 26.
As shown in Figure 19 and Figure 19 A, reaction box 100 includes top board 2, base plate 4, left plate 6, right plate 8, panel 10 and the back of the body
Plate 12.The substantially square box-like body of reaction box 100, is made up of plastic material,
As one kind preferably, as shown in figure 19, the left plate 6 of described reaction box 100 or one end of right plate 8 are an inclined-plane
22.Preferably, the backboard 12 of reaction box 100 is provided with an extension 24, and extension 24 extends out to the outside on inclined-plane 22.Preferably
Ground, inclined-plane 22 is arranged on the lower left corner of reaction box 100.In one embodiment, backboard 12 is square, and panel 10 is five sides
Shape, left plate 6 has one side and an inclined-plane, the rectangular triangle of extension 24, and the hypotenuse of right angled triangle is backboard 12
Junction with extension 24.Inclined-plane 22 and the setting of extension 24, are easy to artificially distinguish the positive and negative of reaction box 100, it is to avoid
Reaction box 100 is reversely inserted in detection box 200, on the other hand, when reaction box is with detection box cooperation, can be anti-with automatic identification
Answer the positive and negative of box, it is to avoid maloperation.
As one kind preferably, as shown in figure 20, reaction box 100 is provided with positioning convex 16.Positioning convex 16 is arranged on face
On plate 10, positioning convex 16 is in upside-down triangle shape, such as Figure 20 A, and positioning convex 16 is used for reaction box 100 fixedly positioning in detection
In the corresponding groove of box 200, positioning convex 16 is spacing with groove to be engaged, and so that reaction box 100 is fastened in detection box 200, keeps away
When exempting from rotation, reaction box 100 detects the displacement of box 200 relatively.
As one kind preferably, as shown in Figure 19 A, the top board 2 of reaction box 100 is provided with handle 14, in detection, convenient use
Family is held quick insert reaction box 100 of handle 14 and is detected in box 200, upon completion of the assays, and quickly pulls out reaction box 100
Go out.As one kind preferably, the backboard 12 of reaction box 100 is provided with notch 52, and the setting of notch 52 makes being molded into of reaction box 100
Type is more convenient.
As a kind of specific embodiment, as shown in Fig. 5, Fig. 6 and Figure 22, in reaction box 100, it is provided with reagent storage part
102nd, reagent release portion 150, reacting part, detection zone 28, sampling rod 30 and liquid absorption pad 32, reaction box 100, reagent storage part
102nd, reagent release portion 104 and sampling rod 30 are made by plastic material.Sampling rod 30 is used for gathering fluid sample such as blood, urine
Liquid etc., and fluid sample is added in reaction box 100.Upon completion of the assays, liquid absorption pad 32 is used for Recycling of waste liquid, it is to avoid
Liquid leakage pollutes.
As shown in Fig. 5, Fig. 6 and Figure 22, reaction box 100 is a cavity, can be divided into box body 18 and upper lid 20, box body 18 includes
Backboard 12, base plate 4, left plate 6 and right plate 8, upper lid 20 includes top board 2 and panel 10, and upper lid 20 and box body 18 pass through welding
It is tightly connected etc. mode, facilitate the assembling of reaction box 100.Described liquid absorption pad 32 is arranged on the top on inclined-plane 22, and liquid is inhaled
Receiving the turn right successively direction of side plate 8 of pad 32 is sampling rod 30 and reagent storage part 102, and reacting part and reagent release portion 104 are all provided with
Put the lower section in reagent storage part 102, detection zone 28 is arranged in reacting part.Sampling rod 30 is arranged on reaction box by an opening
In 100, sampling rod 30 and the cooperation of this opened gap, it is to avoid during sampling, being mixed into of foreign body.It is left that inclined-plane 22 is arranged on sampling rod 30
Side, is conducive to the sample on sampling rod 30 to be fully contacted with reactant liquor, it is to avoid reactant liquor is very few and does not reach sample.Liquid is inhaled
Receipts pad 32, reagent storage part 102, the general layout arrangement of sampling rod 30, reacting part and reagent release portion 104 are not limited to upper type.Excellent
Selection of land, the second drainage plate 44 is connected with first baffle 48 upper end, and the upper end of the first drainage plate 42 is also associated with sampling needle guide plate
57, sampling needle guide plate 57 vertical direction is arranged.As shown in Figure 12 and Figure 20 B, box body 18 and upper lid 20 are equipped with sampling needle admittedly
Determine part 59, for sampling needle fixture 59, it is to avoid it rocks.
As a kind of specific embodiment, reagent storage part and reaction box connected mode, as shown in Figure 8 A, reagent storage part
102 are provided with keeper, and each keeper constitutes cavity, and this cavity is used for housing reagent storage part 102 so that reagent storage part
102 are fixedly mounted in reaction box 100.Described cavity can also adopt routine techniquess means of the prior art.As one
Specific embodiment, as shown in Figure 12 and 12A, Figure 20 and Figure 20 B, panel 10 is provided with locating dowel 54 and gripper shoe 61, and left side is fixed
Position plate 56 and right positioner plate 58 are multiple scattered keepers, scattering device box body 18 and on lid 20, above-mentioned keeper
For being fixedly connected reagent storage part 102.As shown in Figure 8 A and 8 B, the keeper of reagent storage part 102 includes installing hole
122nd, left keeper 124, right keeper 126 and lower keeper 128.As shown in figure 21, installing hole 122 is buckled in locating dowel 54,
Upper end for fixating reagent storage part 102;Left keeper 124 and right keeper 126 respectively with left positioner plate 56 and right side
The spacing connection of location-plate 58, lower keeper 128 is placed in gripper shoe 61.Therefore, reagent storage part 102 is stably mounted at instead
Answer in box 100, it is to avoid reagent storage part 102 is mobile in reaction box 100 or displacement during rocking.
Above-mentioned each can arbitrarily be matched as needed preferably and embodiment between and be combined, final to realize
The purpose of quick sample Concentration Testing.
As shown in figure 23 and figure 24, it is provided with a push rod 204 in detection box 200, push rod 204 passes through to open described on reaction box
Mouth 34 is contacted with forced section and provides the active force of external equipment.
As one kind preferably, described push rod 204 can detection box 200 relatively move.Push rod 204 can be fixed and be positioned on box body
Upper it is also possible to box body relative motion.During push rod 204 activity, by the zone of action of peripheral equipment control push rod 204 and movement
Position, for example, can use the movement of motor control push rod 204, of the prior art routine techniquess may also be employed.If push rod 204 is solid
It is scheduled on box body, when reaction box 100 inserts the detection box 200 of external equipment, by insertion force, push rod 204 is joined with push rod 152
Close, make push rod 152 action.
As one kind preferably, described push rod 204 is arranged on the base plate of detection box 200 or described push rod 204 is arranged on inspection
Survey in the inner panel of box 200.As shown in figure 25, during the bottom surface that detection box 200 is push rod 152 with forced section, opening 34
It is arranged on the base plate 4 of reaction box 100, push rod 204 is arranged on the base plate of detection box 200.Preferably, the upper end of push rod 204
Portion is provided with chamfering, facilitates push rod 204 to stretch into opening 34.When the back that detection box 200 is push rod 152 with forced section coordinates, opening
34 are arranged on the backboard 12 of reaction box 100, and push rod 204 is arranged in the inner panel of detection box 200.
As a kind of preferred, as shown in Figure 24 and Figure 26, it is provided with a portable plate in detection box 200, portable plate includes substrate
206 and elastic component 210, substrate 206 is fixedly connected on the inwall of detection box 200 by elastic component 210.Preferably, elastic component
210 quantity is two, and elastic component 210 includes installed surface 212 and two elastic arms 214, and installed surface 212 is interior with detection box 200
Side is fixedly connected, and two elastic arms 214 are fixedly connected with substrate 206 respectively.
As one kind preferably, described substrate 206 is heating plate, is designated as the first heating plate.I.e. portable plate is i.e. as heating member
Simultaneously again as elastic fastener.
As one kind preferably, as shown in Figure 23 27, one of medial surface of described detection box 200 is provided with shell fragment 216.
Preferably, shell fragment 216 is arranged on one of medial surface adjacent with portable plate.Shell fragment 216 is provided with an elastic arm 218, elastic arm
218 one end are fixing with shell fragment 216, and the other end of elastic arm 218 is in smooth curved surface shape, and elastic arm 218 is towards detection box 200 internal cavities
Install.Shell fragment 216, in order to the inclined-plane 22 on complex reaction box 100, makes the side being not provided with inclined-plane on recoil case 100 be close to inspection
Survey the medial wall of box 200.Because shell fragment 216 has certain elasticity, if reaction box 100 is reversely inserted in detection box 200
When, due to the effect of shell fragment 216, reaction box 100 cannot plug together completely in detection box 200, therefore, shell fragment 216 and inclined-plane 22
Cooperation again can play the recognition reaction that reaction box 100 is correctly inserted into.
As one kind preferably, as shown in figure 23, one of medial surface of described detection box 200 is provided with groove 202.Excellent
Selection of land, groove 202 is arranged on the medial surface of relative motion plate.Groove 202 is used for the positioning convex on clamping reaction box 100
16, so that the reaction box 100 can be connected on groove 202, it is to avoid detection box 200 in rotation or vibration processes, reaction box 100
Displacement.
As a kind of specific embodiment, as shown in Fig. 4, Figure 23 and Figure 24, detection box 200 is fixed on external equipment,
To realize mixing or to rotate, detection box 200 includes the box body of open at one end, and box body is provided with light hole 201, is provided with top in box body
Bar 204, the first heating plate 206 and the second heating plate 208, the first heating plate 206 is portable plate, and push rod 204 passes through above-mentioned opening
34 are contacted with forced section and provide external thrust, and the first heating plate 206 and the second heating plate 208 are used for heating reagent, to meet
The requirement of reaction temperature.
As shown in figure 25, when reaction box 100 is inserted in detection box 200, reaction box 100 presses the first heating plate 206,
First heating plate 206 under pressure, makes elastic component 210 deform upon, and reaction box 100 can quick insertion by convex
Play 16 to fasten in groove 202.Meanwhile, the inclined-plane 22 compression shell fragment 216 of reaction box 100, makes the detection zone 28 on reaction box 100
Light hole 201 on aim detecting box 200.During reaction box 100 is inserted into detection box 200, push rod 204 stretches into opening
34 are contacted with push rod 152, with reaction box 100 from top to bottom during, push rod 152 action from lower to upper, carry dynamic seal
104 actions from lower to upper, make sealing member 104 tear off with reagent storage part 102, and release reagent is as shown in Figure 7 A.
On existing market face, glycolated hemoglobin detection method is a lot, and wherein conventional detection method has ion exchange layer
Analysis, affinity chromatograph, high-pressure liquid phase, immunization, Ion capture, electrophoresis method etc..After immunization refers to erythrocytolysis, it is based on
The interaction of antigen molecule and distinct antibodies is measuring HbA1c.
Include following two detecting steps with immune agglutination method detection HbA1c:Total hemoglobin in detection sample respectively
Hb concentration and Glycohemoglobin HbA1c concentration.The detection of total hemoglobin (Hb) aoxidizes blood red egg by using the potassium ferricyanide
Ferrous ion in white, generates metahemoglobin, it is blood red that metahemoglobin and rhodanide reaction generate Hydrogen thiocyanate high ferro
Albumen, light absorption value under 531nm for the detection, obtain the concentration of Hb.Glycolated hemoglobin (HbA1c) is exempted from containing multiple HbA1c
HbA1c competition binding in the agglutinin in epidemic disease reaction bonded site and blood is marked at the anti-HbA1c antibody on latex beadses, front
The combination of person can cause the change of reactant liquor turbidity, can obtain HbA1c in blood by the extinction value changes detecting 531nm
Concentration.In blood, the concentration of HbA1c is also high, and turbidity is lower, and light absorption value is less, and light absorption value passes through school with the change of HbA1c concentration
Directrix curve obtains.
Therefore, immunization detection HbA1c need using rhodanate liquid reagent (Buffer), marked anti-HbA1c resist
The latex balloon of body, potassium ferricyanide dried reagent (drying object) and the agglutinin containing multiple HbA1c immunoreation binding sites are done
Dry reagent (drying object).As shown in figure 28, rhodanate liquid reagent 60 is stored in reagent accommodating chamber 108, marked anti-HbA1c
The latex balloon 62 of antibody is stored in reagent accommodating chamber 106, and potassium ferricyanide dried reagent 64 is solidificated on second reaction zone 25, coagulates
The plain dried reagent 66 of collection solidifies in the first reaction zone 26.
Wherein, latex balloon is latex lyophilizing bead, will connect upper spy by covalently bound method in advance in latex bead
Different in nature HbA1c antibody, then be volume identical bead it is achieved that to latex antibody response using freeze drying technology by latex balloon quick-freezing
The maximization of active protection, meanwhile, greatly improves room temperature storage life of lower effect duration.
Detect the concentration of HbA1C in blood using reaction box of the present utility model.Immunization detects the step of HbA1c such as
Under:
Step 0:As shown in figure 28, the sampling rod 30 with sample is inserted in reaction box.
Step 1:As shown in Figure 28 A, rotation detection box, reaction box is inserted in detection box, push rod 152 is in external thrust
In the presence of move upwards, tear off sealing member 104, latex balloon 62 and rhodanate 60 discharge simultaneously, and latex balloon 62 falls through into
Temporary in first reaction zone 26, rhodanate liquid reagent 60 falls through in detection zone 28 in second reaction zone 25.
Step 2:As shown in Figure 28 B, revolving reaction box, by Buffer60 (rhodanate), drying object 64 (potassium ferricyanide)
With blood sample mixing, form mixture X.
Step 3:As shown in fig. 28 c, revolving reaction box, the mixture X in step 2 is rotated to detection zone 28, detects sample
The content of hemoglobin (Hb) in product.
Step 4:As shown in fig. 28d, continue revolving reaction box, make the mixture X in step 2 enter the first reaction zone 26,
Mixture (marked anti-with drying object 66 (agglutinin containing multiple HbA1c immunoreation binding sites) and latex balloon 62
The latex beadses of HbA1c antibody) mixing, form mixture Y.
Step 5:As shown in Figure 28 E, revolving reaction box, make the mixture Y in step 4 enter detection zone 28, detection sample
The content of middle glycolated hemoglobin (HbA1C).
Step 6:As shown in Figure 28 F, revolving reaction box, make the mixture Y in step 4 enter liquid absorption pad 32, make anti-
Waste liquid after answering is absorbed.
HbA1c detection terminates, and is calculated detection structure and exported by external equipment.
Reaction box of the present utility model is not limited to the detection of HbA1c in above-mentioned blood sample it is also possible to be used for other biological
The detection of sample such as urine, saliva, spinal fluid etc. is it can also be used to C reactive protein, cholesterol, blood fat and blood glucose etc. in biological specimen
The Concentration Testing of analyte.
In described above, all plus special instruction, all using routine techniquess means of the prior art.
Claims (10)
1. a kind of detection reaction box, including the reagent storing device being arranged in reaction box and sampling rod, reagent storing device is provided with
Reagent release position, described reaction box includes the wallboard towards reagent release position it is characterised in that described wallboard is provided with water conservancy diversion
Drop contact on muscle, fuisw rib and reagent release position end, fuisw rib is used for the drop on water conservancy diversion reagent release position end.
2. detection reaction box according to claim 1 is it is characterised in that described fuisw rib is connect with reagent release position end
Touch.
3. detection reaction box according to claim 1 is it is characterised in that described reaction box also includes reacting part, fuisw rib
Reach in reacting part.
4. according to claim 1 detection reaction box it is characterised in that described reaction box includes side plate, described water conservancy diversion
Muscle is with side plate at an angle.
5. detection reaction box according to claim 1 is it is characterised in that described fuisw rib is domed.
6. detection reaction box according to claim 1 is it is characterised in that described fuisw rib is included towards reagent release position
Guide face, described guide face is smooth curved surface.
7. according to claim 6 detection reaction box it is characterised in that described fuisw rib also include adjacent with guide face
Two sidepiece guide faces, the junction of two sidepiece guide faces and wallboard is smooth curved surface.
8. detection reaction box according to claim 1 is it is characterised in that described reagent release position includes reagent liberation port.
9. detection reaction box according to claim 8 is it is characterised in that described reagent release position also includes being connected to reagent
Deflector below liberation port.
10. detection reaction box according to claim 1 is it is characterised in that described sampling rod is arranged on inside reaction box.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201620733260.4U CN205941571U (en) | 2016-07-07 | 2016-07-07 | Detect reaction box |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201620733260.4U CN205941571U (en) | 2016-07-07 | 2016-07-07 | Detect reaction box |
Publications (1)
Publication Number | Publication Date |
---|---|
CN205941571U true CN205941571U (en) | 2017-02-08 |
Family
ID=57928600
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201620733260.4U Active CN205941571U (en) | 2016-07-07 | 2016-07-07 | Detect reaction box |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN205941571U (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109701408A (en) * | 2017-10-25 | 2019-05-03 | 台达电子工业股份有限公司 | Particles aggregate mixed method and mixing apparatus |
CN110233952A (en) * | 2018-03-06 | 2019-09-13 | 株式会社理光 | Assemble the configuration structure of substrate, the configuration structure and photographic device of photographing element substrate |
CN115372615A (en) * | 2022-10-25 | 2022-11-22 | 艾康生物技术(杭州)有限公司 | Detection device |
-
2016
- 2016-07-07 CN CN201620733260.4U patent/CN205941571U/en active Active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109701408A (en) * | 2017-10-25 | 2019-05-03 | 台达电子工业股份有限公司 | Particles aggregate mixed method and mixing apparatus |
CN110233952A (en) * | 2018-03-06 | 2019-09-13 | 株式会社理光 | Assemble the configuration structure of substrate, the configuration structure and photographic device of photographing element substrate |
CN115372615A (en) * | 2022-10-25 | 2022-11-22 | 艾康生物技术(杭州)有限公司 | Detection device |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105013546B (en) | Biological sample reaction box | |
CN105021544B (en) | Detecting system | |
CN107942050B (en) | A kind of detection method of microfluidic chip based on magnetic bead technology | |
CN205941571U (en) | Detect reaction box | |
CN205049571U (en) | Reaction box | |
JPH04504758A (en) | cuvette | |
CN204816573U (en) | Biological sample reaction box | |
CN204855520U (en) | Biological sample analysis box | |
CN204855358U (en) | Assay system | |
CN105435867B (en) | Detect the magnetic microparticle chemiluminescence micro-fluidic chip of creatine kinase isozyme in whole blood | |
CN204832194U (en) | Biological sample testing cassette | |
CN204832049U (en) | Detect box | |
CN105021807B (en) | Reagent reacting box and the application in biology sample detection thereof | |
CN204832204U (en) | Reagent reaction box | |
EP3752290A1 (en) | Diagnostic assay cartridge for conducting multiple diagnostic assays on a patient's single liquid test sample and methods of use related thereto | |
CN106124780B (en) | Detect reaction box | |
US11474120B2 (en) | Separable cassette for measuring glycated hemoglobin | |
CN105137091B (en) | Reagent storing device | |
CN204832203U (en) | Reagent memory | |
CN205599183U (en) | Creatine kinase isoenzyme's magnetic particle chemiluminescence micro -fluidic chip in detection whole blood | |
CN108548932A (en) | Thyrotropic hormone TSH kits and preparation based on micro-fluidic chip and detection method | |
JPH0882622A (en) | Occult blood-judging apparatus | |
JP3487685B2 (en) | Fecal occult blood determination device | |
JP3519831B2 (en) | Fecal occult blood determination device | |
US20180321271A1 (en) | Liquid test sample injection device and kits and methods of use related thereto |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant |