CN205933848U - Catalase determination of activity appearance - Google Patents
Catalase determination of activity appearance Download PDFInfo
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- CN205933848U CN205933848U CN201620610666.3U CN201620610666U CN205933848U CN 205933848 U CN205933848 U CN 205933848U CN 201620610666 U CN201620610666 U CN 201620610666U CN 205933848 U CN205933848 U CN 205933848U
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Abstract
The utility model provides a catalase determination of activity appearance, is reaction cylinder (4) that have lid (11), and the lid center has according to pivot (14), epaxial and the axle is other has protruding bolt (15) and bayonet socket seat (12), and epaxial cover has spring (13), covers gas -liquid import (17) in addition, has survey oxygen hole (6) of taking open/close valve on the reaction cylinder lateral wall, leads to liquid gas pocket (5) and tapping hole (1), just all has sealing washer (18). Depositing liquid ware (10), film (9), aperture reposition of redundant personnel supporting spring (8) and stirring sieve (2) in the reaction cylinder, all having opened branch discharge orifice or sieve mesh (16, 19, 3) on the disc, platform (7) is put on reaction cylinder inside wall upper portion. The utility model discloses can record the data of reaction overall process, the precision is high, with producing oxygen volume reflection enzymatic activity, is one set of brand -new technical scheme.
Description
Technical field
This utility model is related to a kind of chemical experimental instrument, is particularly used for the gas generated increase and decrease of enzymatic reaction and becomes
Change, indirectly show analyzer and its using method of enzymatic activity.
Background technology
The mensure of catalase activity, is by hydrogen peroxide and catalase peroxide in enzymatic reaction all the time
Change the minimizing of hydrogen amount, with the ultraviolet light of ultraviolet spectrophotometer 240nm wavelength, absorbance is gradually lowered, and measures absorbance
Pace of change, measure catalatic activity.Specific practice is that catalase and hydrogen peroxide are placed in cuvette,
Immediately spectrophotometer is also placed in cuvette, opens photometer, cover the lid of cuvette, carry out colorimetric determination.This ratio
When color instrument is measured, throws in catalase and almost carry out with throwing in hydrogen peroxide simultaneously, because of the enzyme tool in enzymatic reaction
There is high efficiency, once both mix, and reaction begins to, the reaction loss of hydrogen peroxide has also produced, even if throw in immediately dividing
Light photometer, start survey, still cannot reflect the waste of hydrogen peroxide in this time difference, surveyed data is less accurate,
Error is larger.This utility model is that the increase and decrease of catalase and oxygen yield in hydroperoxidation is reflected peroxidating
The activity of hydrogen enzyme, reaction is synchronous with actual measurement to be carried out, and has not both had time difference, can show the overall picture entirely reacting again, for this reason, designing
The new using method going out a kind of special analyzer and providing set of complementary, through retrieval and market survey, there are no related money
Material report and material object appear on the market.
Utility model content
The technical problems to be solved in the utility model is that offer is a kind of to be changed to the decrement of hydrogen peroxide in enzymatic reaction
The increment of oxygen reflecting catalatic activity, can make a kind of hydrogen peroxide that reaction and actual measurement are synchronously carried out and error is little
Enzyme assay instrument and its using method.
Solve above-mentioned technical problem to adopt the following technical scheme that：This catalase activity analyzer, including reaction and survey
Determine part, the lid of liner sealing ring is arranged at the reaction tube top in cylinder, cover the gas-liquid import with opening and closing valve, this lid center
There is axis hole and the convex bolt of upper bands is installed and is cased with back-moving spring by rotating shaft, the axis hole both sides covering are provided with bayonet mount, seat
On have bayonet socket, have dowel hole by the middle epimere and lower end section of rotating shaft；Oxygen determination pore, logical liquid gas are provided with the side wall of reaction tube
Hole and taphole, this three hole all with opening and closing valve, the upper inside wall of reaction tube has annular shelves, in reaction tube from top to bottom
There are liquid storage ware, film, aperture shunting supporting spring and stirring sieve, have on liquid storage ware and on film that axis hole is identical with aperture, position correspondence
Large shunt hole, aperture shunting supporting spring has axis hole and a little tap hole, stirring sieve has axis hole and sieve aperture, liquid storage ware and film are put
It is placed in shelves, aperture shunting supporting spring and stirring sieve all have and the corresponding alignment pin in dowel hole position by rotating shaft, reaction tube
It is mounted on sealing ring with around extraneous through hole on lid and side wall.
The aperture ratio of described liquid storage ware axis hole presses the big 1-2mm of shaft diameter, and the aperture ratio of film axis hole presses shaft diameter
Big 0.1-0.2mm, the aperture ratio that aperture shunts supporting spring axis hole presses the big 0.1-0.2mm of shaft diameter.
Described liquid storage ware, film, aperture shunt large shunt hole on supporting spring and stirring sieve or little tap hole by outer and interior
All in concentric arrays, along lonizing radiation directional spreding.
The internal diameter of described reaction tube is than the external diameter of liquid storage ware and the big 1-2mm of the diameter of film；Described aperture shunting support
The tangent line making camber line outside each large shunt hole of concentric arrays with diameter greater than liquid storage ware or film most peripheral of piece connects circle
Diameter, and the internal diameter less than annular shelves.
The beneficial effects of the utility model are：This analyzer can make reaction synchronously carry out with actual measurement, and surveyed data can reflect
Go out the overall picture of whole reaction, high precision, error are little, and provide a kind of new instrument of enzyme assay, new approaches and new making
Use method.
Brief description
Fig. 1 is this analyzer structural representation.
Fig. 2 is that the large shunt hole arrangement state on this analyzer liquid storage ware and supporting spring illustrates (vertical view) figure.
Fig. 3 is that the little tap hole layout state that this analyzer aperture shunts on supporting spring illustrates (vertical view) figure.
Specific embodiment
This utility model is described in detail with reference to embodiment and referring to the drawings：Referring to Fig. 1-3, this catalase activity
Analyzer, including reaction and components of assays, there is the lid 11 of liner sealing ring on reaction tube 4 top in cylinder, has covered band and opened
The gas-liquid import 17 of valve closing, there is axis hole a at this lid center is provided with the convex bolt of upper bands 15 and is cased with back-moving spring 13 by rotating shaft
14, the axis hole both sides covering are provided with bayonet mount 12, and seat has bayonet socket, have dowel hole by the middle epimere and lower end section of rotating shaft；
Oxygen determination pore 6, logical liquid pore 5 and taphole 1 are provided with the side wall of reaction tube, this three hole all carries keying valve, reaction tube
Annular shelves 7 are had on upper inside wall, in reaction tube, from top to bottom has liquid storage ware 10, film 9, aperture shunting supporting spring 8 and stirring
Sieve 2, has on liquid storage ware and on film that axis hole is identical with aperture, position corresponding large shunt hole 16, and aperture shunts has axle on supporting spring
Hole and little tap hole 19, stirring sieve have axis hole and sieve aperture, liquid storage ware and film to place in shelves, aperture shunts supporting spring and stirs
Mixing sieve all has and the corresponding alignment pin in dowel hole position 20 by rotating shaft, react on cover and side wall with extraneous through hole around
It is mounted on sealing ring 18.
The aperture ratio of described liquid storage ware axis hole a presses the big 1-2mm of shaft diameter, and the aperture ratio of film axis hole presses shaft diameter
Big 0.1-0.2mm, the aperture ratio that aperture shunts supporting spring axis hole presses the big 0.1-0.2mm of shaft diameter.
Described liquid storage ware, film, aperture shunt large shunt hole 16 on supporting spring and stirring sieve or little tap hole 19 by outer
And interior all in concentric arrays, along lonizing radiation directional spreding.
The internal diameter of described reaction tube 4 is than the external diameter of liquid storage ware 10 and the big 1-2mm of the diameter of film 9；Described aperture divides
The tangent line of each large shunt hole 16 outside camber line making concentric arrays with diameter greater than liquid storage ware or film most peripheral of stream supporting spring 8 connects
Connect the diameter of round b, and the internal diameter less than annular shelves 7.
Using before this analyzer, first check analyzer, the bottom surface of liquid storage ware 10 is overlapped with film 9, frame rests on circle together
In annular shelves 7, rotating shaft 14 is pressed in lifting upwards, counterclockwise slightly turns this axle, takes advantage of a situation and will rest on by the convex bolt 15 in rotating shaft
Position on bayonet mount 12, now, aperture shunting supporting spring is close to the film of top, and stirring sieve 2 is up, on aperture shunting supporting spring 8
The large shunt hole 16 not insertion that each aperture is overlapped with the two-layer above it, though then liquid storage ware have liquid also will not be from little shunting
Flow downward out in hole；Turn again by rotating shaft, make large shunt hole and little tap hole insertion, then the liquid of liquid storage ware will be by large shunt
Kong Zhi little tap hole flows downward out, and the flow of unit interval is less.When needing flow big, turn clockwise by rotating shaft, make convex bolt from
Depart from the bayonet mount put, press still further below by rotating shaft, make aperture shunt supporting spring and sieve descending, then aperture shunting supporting spring with stirring
With film separation, the liquid in liquid storage ware will not stopped by little tap hole, flow is just big by large shunt hole to dirty.
To continuously pressing repeatedly by rotating shaft, back-moving spring 13 is rebounded with corresponding number of times, then the repeatedly activity up and down of stirring sieve, makes reaction tube
The reactant liquor of 4 bottoms is more rapid, uniform in abundant agitation, thoroughly complete reaction.Hydrogen peroxide can add from logical liquid pore 5
To reaction tube bottom, catalase adds to liquid storage ware from gas-liquid import 17, and oxygen determination pore 6 is connected with oxygen analyser, tapping
Hole 1 is used for the waste liquid after bleeding off experiment, is that next group experiment is prepared.Lid 11 is used for installing the inside and outside zero of this analyzer
Part, replaces inner body, cleaning reaction tube interior room.
During use, the keying valve of the taphole 1 of reaction tube bottom cuts out, oxygen determination pore 6 is connected with oxygen analyser, open logical
Valve on liquid pore 5 and gas-liquid import 17, turns carrying upwards by rotating shaft 14 and making low-angle counterclockwise, so that aperture is shunted
Supporting spring is close to film thereon, large shunt hole 16 and the not insertion of little tap hole 19, and convex bolt 15 rests on bayonet mount 12, will cover 11 passes
Well, it is screwed to sealing.Add the hydrogen peroxide liquid through metering from logical liquid pore 5, from gas-liquid import 17 to anti-into reaction tube 4
Answer the liquid storage ware 10 in cylinder to add hydrogen peroxide enzyme liquid through metering, close the valve of logical liquid pore 5 and gas-liquid import 17 respectively,
Now, the hydrogen peroxide enzyme liquid in liquid storage ware 10 is not mixed with hydrogen peroxide downwards, and enzymatic reaction not yet starts, from oxygen determination
Read and record original oxygen content in reaction tube in instrument.To slow reaction, then above carry by rotating shaft and low-angle is clockwise
Turn this axle, make large shunt hole and little tap hole insertion, then the hydrogen peroxide enzyme liquid in liquid storage ware is along large shunt hole, again to little point
Discharge orifice flows downward out, the hydroperoxidation with reaction tube bottom, constantly, step by step consume hydrogen peroxide while, produce
Oxygen, time segment reads respectively and records the increment of oxygen, until reaction terminates, draw the oxygen content of different time sections
Change curve, calculates catalatic activity according to formula.If needing fast reaction, be further continued for turning clockwise by turn
Axle, after making the convex bolt on this axle depart from bayonet mount, then will be pressed downwards by rotating shaft, make aperture shunt supporting spring departing from offset plate, liquid storage
Catalase in ware is quickly dirty through large shunt hole, is not stopped by little tap hole, be flowed directly to reaction in downflow processes
Cylinder bottom, is mixed with the hydrogen peroxide in cylinder bottom, enzymatic reaction starts, is recorded the oxygen content of each time period as aforementioned process
Change and formulate curve.If making reaction faster, turning by rotating shaft, making aperture shunt supporting plate and departing from film, hydrogen peroxide enzyme liquid
It flow to reaction tube bottom directly down from large shunt hole to mix with hydrogen peroxide and react, continuously repeatedly press by turning simultaneously more up and down
Axle, make stirring sieve rapidly stir up and down, mixed liquor is stirred, then reaction can faster, more thorough.As catalatic
Determination of activity is intended to negate and then should preferably adopt above-mentioned the third by the quick tapping in large shunt hole and to stir same once should be quick
The method of Shi Jinhang.At the beginning of reaction starts, oxygen yield is larger, and the slope of curve is also big, and the activity of reflection enzyme is best.
The computing formula of catalase activity is：
Catalase activity (U μm of ol min-1·g-1)=(2 × Δ O2×VT)/(W×VS×t)；
The enzyme amount of 2 times of the amount of the material being increased with interior oxygen per minute is for 1 enzyme-activity unit (U)；
In formula：ΔO2Oxygen incrementss (μm ol) for the straight line portion putting oxygen curve of Catalase solution to be measured, VT
For extracting enzyme liquid cumulative volume (mL), VSFor taking enzyme liquid volume (mL) when measuring, W is biomaterial fresh weight (g), and t is mistake to be measured
The time (min) that the straight line portion putting oxygen curve of hydrogen oxide enzymatic solution is consumed.
As for using a pair of bayonet mount, having little tap hole is to leave room for for setting up gear and regulation and control tapping rate, to expand
The versatility of this analyzer.The size axis hole a of different parts, insertion and not insertion, whether need to position, if take into account airtight temporarily
Property etc. all should be considered as a whole.The pore size of large shunt hole and little tap hole, density, distribution mode, can be diversified, does not limit to
Several ways shown in the drawings, in a word, are size based on the transversal disc of reaction tube, the size of load responsive fluid, macropore and aperture
Depending on whether the conversion of insertion and not insertion the factor such as facilitates.Liquid storage ware, film, aperture splitter, all can make on demand
The different series of products of arrangement mode, pore size, distribution density, are exchanged by the requirement of differential responses.Supporting application
Oxygen analyzer such as precision prescribed is high, then can choose the oxygen electrode of the Chlorolab2 type of Britain Hansatech production.
Claims (4)
1. a kind of catalase activity analyzer, including reaction and components of assays, is characterized in that the reaction tube in cylinder（4）
The lid of liner sealing ring is arranged at top（11）, cover the gas-liquid import with opening and closing valve（17）, there is axis hole at this lid center（a）And install
There is the convex bolt of upper bands（15）Be cased with back-moving spring（13）By rotating shaft（14）, the axis hole both sides covering are provided with bayonet mount
（12）, seat has bayonet socket, has dowel hole by the middle epimere and lower end section of rotating shaft；On the side wall of reaction tube, oxygen determination pore is installed
（6）, logical liquid pore（5）And taphole（1）, this three hole all with opening and closing valve, the upper inside wall of reaction tube has annular shelves
（7）, in reaction tube, from top to bottom have liquid storage ware（10）, film（9）, aperture shunting supporting spring（8）With stirring sieve（2）, on liquid storage ware
Axis hole is identical with aperture, position corresponding large shunt hole with having on film（16）, aperture shunting supporting spring has axis hole and little shunting
Hole（19）, stirring sieve has axis hole and sieve aperture, liquid storage ware and film to place in shelves, aperture shunting supporting spring and stirring sieve all have
With the corresponding alignment pin in dowel hole position by rotating shaft（20）, reaction cover and side wall are respectively mounted with around extraneous through hole
There is sealing ring（18）.
2. catalase activity analyzer as claimed in claim 1, is characterized in that described liquid storage ware axis hole（a）Aperture
By the big 1-2mm of shaft diameter, the aperture ratio of film axis hole presses the big 0.1-0.2mm of shaft diameter to ratio, and aperture shunts supporting spring axis hole
Aperture ratio presses the big 0.1-0.2mm of shaft diameter.
3. catalase activity analyzer as claimed in claim 1, is characterized in that described liquid storage ware, film, aperture divide
Large shunt hole on stream supporting spring and stirring sieve（16）Or little tap hole（19）By outer and interior all in concentric arrays, along lonizing radiation
Directional spreding.
4. catalase activity analyzer as claimed in claim 1, is characterized in that described reaction tube（4）Internal diameter ratio deposit
Liquid ware（10）External diameter and film（9）The big 1-2mm of diameter；Described aperture shunting supporting spring（8）With diameter greater than liquid storage ware or
Film most peripheral makees each large shunt hole of concentric arrays（16）The tangent line of outside camber line connects circle（b）Diameter, and less than circle
Annular shelves（7）Internal diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620610666.3U CN205933848U (en) | 2016-06-21 | 2016-06-21 | Catalase determination of activity appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620610666.3U CN205933848U (en) | 2016-06-21 | 2016-06-21 | Catalase determination of activity appearance |
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| Publication Number | Publication Date |
|---|---|
| CN205933848U true CN205933848U (en) | 2017-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620610666.3U Active CN205933848U (en) | 2016-06-21 | 2016-06-21 | Catalase determination of activity appearance |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086157A (en) * | 2016-06-21 | 2016-11-09 | 俞率成 | Catalase activity analyzer and using method thereof |
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2016
- 2016-06-21 CN CN201620610666.3U patent/CN205933848U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086157A (en) * | 2016-06-21 | 2016-11-09 | 俞率成 | Catalase activity analyzer and using method thereof |
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