CN205898641U - Flow cytometry detecting tube temperature regulating device - Google Patents
Flow cytometry detecting tube temperature regulating device Download PDFInfo
- Publication number
- CN205898641U CN205898641U CN201620879345.3U CN201620879345U CN205898641U CN 205898641 U CN205898641 U CN 205898641U CN 201620879345 U CN201620879345 U CN 201620879345U CN 205898641 U CN205898641 U CN 205898641U
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- Prior art keywords
- chilling plate
- semiconductor chilling
- semiconductor refrigeration
- temperature
- refrigeration piece
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- 230000001105 regulatory effect Effects 0.000 title claims abstract description 15
- 238000000684 flow cytometry Methods 0.000 title abstract description 9
- 239000004065 semiconductor Substances 0.000 claims abstract description 91
- 238000004458 analytical method Methods 0.000 claims description 16
- 238000005057 refrigeration Methods 0.000 abstract description 15
- 238000010166 immunofluorescence Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 description 16
- 238000005259 measurement Methods 0.000 description 7
- 238000001816 cooling Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
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- 238000007363 ring formation reaction Methods 0.000 description 2
- 102000000412 Annexin Human genes 0.000 description 1
- 108050008874 Annexin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 230000005679 Peltier effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
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- 238000006243 chemical reaction Methods 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model belongs to the technical field of it is biological, concretely relates to flow cytometry detecting tube temperature regulating device, it includes semiconductor refrigeration pipe and controller, the semiconductor refrigeration pipe is including left semiconductor refrigeration piece and right semiconductor refrigeration piece, left side semiconductor refrigeration piece passes through hinge hinge with right semiconductor refrigeration piece, the inner wall of left side semiconductor refrigeration piece or right semiconductor refrigeration piece is provided with temperature sensor, left side semiconductor refrigeration piece and right semiconductor refrigeration piece are the opaque semiconductor refrigeration piece and make, the controller includes power module and program -controlled temperature ware, a left side semiconductor refrigeration piece and right semiconductor refrigeration piece all with controller electric connection, temperature sensor and controller electric connection. The utility model discloses can control the temperature into required temperature effectively, can effectively reduce immunofluorescence cancellation in the flow cytometry testing process to light in weight, small, shockproof, noiselessness, the appearance of going up that may not influence flow cytometer is operated.
Description
Technical field
This utility model belongs to biological technical field and in particular to a kind of FCM analysis pipe temperature regulating device.
Background technology
Flow cytometry (flowcytometry, fcm) is a kind of extremely important modern biology technology, for outstanding
Float on the molecule in fluid (inclusion cell) to be analyzed and sort, be widely used to scientific research and medical science inspection at present
Survey.Flow cytometry needs to use flow cytometer and sheath fluid；Its principle is each particle by high speed sheath liquor stream band
Dynamic, by can be in some way light scattering during laser beam, the fluorescent chemicalses simultaneously being carried be excited and launch frequency
Rate is less than the fluorescence of exciting light.The data splitting of these scattered lights and fluorescence is by detector record it becomes possible to extrapolate each
The physics of grain and chemical property.
When carrying out flow cytometry, if the temperature of streaming sample pipe is uncontrollable, cell function detection number can be caused
According to fluctuation and unstable (data obtaining often has than larger cv value)；Require stricter experiment at some
In, the fluctuation of cell detection data and unstable or even misleading result can be drawn.Or, if the temperature of streaming sample pipe
Degree is uncontrollable to cause cell function unstable, can lead to not tiny function difference that may be present, Yi Jitong between each packet in area
One sample dynamic change in time.Such as, respond the mensure of the intracellular calcium current that environmental stimuli produces for immunocyte, be
The dynamic measurement of one change in fluorescence being directed to time shafts；Strictly control temperature, most important to the accuracy of experiment.For another example
Measure intracellular oxygen-derived free radicals (ros) using dihydro rhodamine (dhr) fluorescent dye, need sample to add under low temperature state
Fluorescent dye, is then brought rapidly up to 37 degree of control isothermal reaction system adding stimulant, receives under conditions of keeping constant temperature
Collect the signal of different time periods, to obtain the data of more accurate, more rich intracellular respiratory burst；So, similar reality
Test and do not require nothing more than constant temperature, and require sample can promptly adjust temperature in the case of machine in real time.Another one example
It is to detect the apoptosis determination experiment that turns up of cell membrane breakage it is desirable to sample keeps 4 degree as best one can using annexin v method
The steady statue of low temperature.
At this stage, the flow cytometer detection pipe carrying out use during flow cytometry is normally at normal temperature environment, and temperature is uncontrollable.
Utility model content
For solving the above problems, the purpose of this utility model is to provide a kind of FCM analysis pipe temperature regulating device, can
So that temperature to be efficiently controlled as required steady temperature.
For achieving the above object, this utility model utilizes the peltier effect of semi-conducting material (i.e. when unidirectional current passes through two
When planting the galvanic couple that different semi-conducting materials are connected into, heat can be absorbed respectively at the two ends of galvanic couple and release heat) convection type
Detection pipe refrigeration or heating, under the cooperation of controller, temperature are efficiently controlled as required temperature.
The detailed technology scheme that this utility model is taken is: a kind of FCM analysis pipe temperature regulating device, it includes partly leading
Body refrigerator pipes and controller；Semiconductor refrigerating pipe includes left semiconductor chilling plate and right semiconductor chilling plate；Left quasiconductor
Cooling piece passes through hinge with right semiconductor chilling plate；The inwall setting of left semiconductor chilling plate or right semiconductor chilling plate
There is temperature sensor；Left semiconductor chilling plate and right semiconductor chilling plate are opaque semiconductor chilling plate and make；Control
Device processed includes power module and type program temperature control；Left semiconductor chilling plate and right semiconductor chilling plate are all electrical with controller
Connect；Temperature sensor is electrically connected with controller.
Further, the end of the end of left semiconductor chilling plate and right semiconductor chilling plate is provided with magnetic piece.Can
So that left semiconductor chilling plate and right semiconductor chilling plate tight closure cyclization, reach inwall and the right side of left semiconductor chilling plate
The purpose that the inwall of semiconductor chilling plate is fitted tightly with flow cytometer detection pipe, such that it is able to effectively further control temperature to be institute
The value needing.
Further, left semiconductor chilling plate or right semiconductor chilling plate be additionally provided with longitudinally through observation window.From
Observation window can observe the surpluses of sample in flow cytometer detection pipe effectively
Further, controller also includes display screen.
Further, type program temperature control is pid thermostat.The related detailed of type program temperature control can be seen by display screen
Carefully；The temperature of such as Current Temperatures sensor measurement, temperature retention time being set by type program temperature control etc., more convenient.
Compared with prior art, this utility model has the advantages that 1, can efficiently control temperature for institute
The steady temperature needing；2nd, in the case of machine on sample is real-time, temperature can be changed at any time according to requirement of experiment；3rd, can be effective
During reducing FCM analysis, immunofluorescence is quenched, and lightweight, small volume, shockproof, noiselessness；4th, can not shadow
Ring the loading operation of flow cytometer.
Brief description
Fig. 1 is the principle sketch of one of this utility model FCM analysis pipe temperature regulating device.
Fig. 2 is the sketch that in this utility model, semiconductor refrigerating pipe is overlooked when using.
Semiconductor refrigerating pipe 1；Left semiconductor chilling plate 11；Right semiconductor chilling plate 12；Hinge 13；Magnetic piece 14；Observation window
15；Controller 2；Power module 21；Type program temperature control 22；Temperature sensor 23；Display screen 24；Flow cytometer detection pipe 3；Loading pin 4；
Sample 5.
Specific embodiment
Refer to Fig. 1, Fig. 2, this utility model is a kind of FCM analysis pipe temperature regulating device, and it includes semiconductor refrigerating
Pipe 1 and controller 2.
Semiconductor refrigerating pipe 1 includes left semiconductor chilling plate 11 and right semiconductor chilling plate 12；Left semiconductor chilling plate
11 is hinged by hinge 13 with right semiconductor chilling plate 12；Left semiconductor chilling plate 11 or the inwall of right semiconductor chilling plate 12
It is provided with temperature sensor 23.
Left semiconductor chilling plate 11 and right semiconductor chilling plate 12 are opaque semiconductor chilling plate and make.
Controller 2 includes power module 21 and type program temperature control 22.
Left semiconductor chilling plate 11 and right semiconductor chilling plate 12 are all electrically connected with controller 2；Temperature sensor 23
It is electrically connected with controller 2.
The operation principle of one of this utility model FCM analysis pipe temperature regulating device is: when preparing to use, streaming
After loading sample 5 in detection pipe 3, left semiconductor chilling plate 11 and right semiconductor chilling plate 12 are surrounded in flow cytometer detection pipe 3
Outer wall, then the loading pin 4 of flow cytometer is inserted sample 5, prepares to complete.
According to experiment demand, by type program temperature control 22 design temperature (when the temperature of temperature sensor 23 measurement is higher than journey
During the temperature that sequence thermostat 22 sets, partly lead to the left by semiconductor chilling plate 11 and the right side for type program temperature control 22 driving power module 21
Body cooling piece 12 is powered, and makes left semiconductor chilling plate 11 and right semiconductor chilling plate 12 refrigeration cooling flow cytometer detection pipe 3, until
The temperature of temperature sensor 23 measurement is equal to the temperature that type program temperature control 22 sets；When the temperature of temperature sensor 23 measurement is less than
During the temperature that type program temperature control 22 sets, type program temperature control 22 driving power module 21 semiconductor chilling plate 11 and right half to the left
Conductor cooling piece 12 is powered, and makes left semiconductor chilling plate 11 and right semiconductor chilling plate 12 heat to heat flow cytometer detection pipe 3,
Until the temperature of temperature sensor 23 measurement is equal to the temperature that type program temperature control 22 sets), after waiting for a period of time, flow cytometer detection
After the temperature stabilization of pipe 3 is design temperature, you can open flow cytometer and carry out flow cytometry.By type program temperature control 22
Sample 5 not only can be made to keep constant temperature, alternating temperature can also be realized；Such as realize protecting by the program of setting program thermostat 22
Hold 36 degrees Celsius after 10 minutes automatic temperature changing be 4 degrees Celsius.
From the above, it is seen that left semiconductor chilling plate 11 and right semiconductor chilling plate 12 not only can freeze but also can make
Heat, can be efficiently controlled temperature for required temperature with cooperation controller 2.
Left semiconductor chilling plate 11 and right semiconductor chilling plate 12 are opaque semiconductor chilling plate and make, permissible
During effectively reducing FCM analysis, immunofluorescence is quenched, and lightweight, small volume, shockproof, noiselessness.
Left semiconductor chilling plate 11 and right semiconductor chilling plate 12 can surround the outer wall in flow cytometer detection pipe 3, loading
When do not need to extract loading pin 4 from flow cytometer detection pipe 3, such that it is able to do not affect flow cytometer loading operate.
The end of the end of left semiconductor chilling plate 11 and right semiconductor chilling plate 12 is provided with magnetic piece 14.A left side is partly led
The magnetic piece 14 of body cooling piece 11 is attracted each other with the magnetic piece 14 of right semiconductor chilling plate 12, can make left semiconductor chilling plate 11 with
Right semiconductor chilling plate 12 tight closure cyclization, reaches the inwall of left semiconductor chilling plate 11 and right semiconductor chilling plate 12
The purpose that inwall is fitted tightly with flow cytometer detection pipe 3, such that it is able to effectively further control temperature to be required value.
Left semiconductor chilling plate 11 or right semiconductor chilling plate 12 be also provided with longitudinally through observation window 15.From
Observation window 15 can observe the surpluses of sample 5 in flow cytometer detection pipe 3 effectively.
Controller 2 can also include display screen 24.The relevant information of type program temperature control 22 can be seen by display screen 24；
The temperature of such as Current Temperatures sensor 23 measurement, temperature retention time being set by type program temperature control 22 etc., more convenient.
Type program temperature control 22 can be pid thermostat.
In sum, temperature can be controlled by one of this utility model FCM analysis pipe temperature regulating device effectively
It is made as required temperature；During FCM analysis can effectively being reduced, immunofluorescence is quenched, and lightweight, small volume,
Shockproof, noiselessness；The loading operation of flow cytometer can not be affected.
It is to be appreciated that term " " center ", " longitudinal ", " horizontal ", "front", "rear", "left", "right", " vertical ", " water
Flat ", " top ", " bottom ", " interior ", the orientation of instruction such as " outward " or position relationship be based on orientation shown in the drawings or position relationship,
It is only for ease of the description present invention and to simplify description, rather than instruction or infer the device of indication or element must have specifically
Orientation, be specific azimuth configuration and operation, thus it is not intended that to the present invention protect content restriction.
Above specific embodiment is only the preferred embodiment of this creation, not in order to limit this creation, all in this wound
Any modification, equivalent substitution and improvement of being done etc. within the spirit made and principle, should be included in this creation protection domain it
Interior.
Claims (5)
1. a kind of FCM analysis pipe temperature regulating device it is characterised in that: include semiconductor refrigerating pipe (1) and controller (2)；
Semiconductor refrigerating pipe (1) includes left semiconductor chilling plate (11) and right semiconductor chilling plate (12)；Left semiconductor chilling plate
(11) pass through hinge (13) with right semiconductor chilling plate (12) hinged；Left semiconductor chilling plate (11) or right semiconductor chilling plate
(12) inwall is provided with temperature sensor (23)；Left semiconductor chilling plate (11) and right semiconductor chilling plate (12) are not
Transparent semiconductor chilling plate is made；Controller (2) includes power module (21) and type program temperature control (22)；Left quasiconductor system
Cold (11) and right semiconductor chilling plate (12) are all electrically connected with controller (2)；Temperature sensor (23) and controller (2)
It is electrically connected with.
2. as claimed in claim 1 a kind of FCM analysis pipe temperature regulating device it is characterised in that: described left semiconductor refrigerating
One end of one end of piece (11) and described right semiconductor chilling plate (12) is provided with magnetic piece (14).
3. as claimed in claim 1 a kind of FCM analysis pipe temperature regulating device it is characterised in that: described left semiconductor refrigerating
Piece (11) or described right semiconductor chilling plate (12) are additionally provided with the observation window (15) running through.
4. described a kind of FCM analysis pipe temperature regulating device as arbitrary in claim 1-3 it is characterised in that: described controller
(2) also include display screen (24).
5. described a kind of FCM analysis pipe temperature regulating device as arbitrary in claim 1-3 it is characterised in that: described program control
Warm device (22) is pid thermostat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879345.3U CN205898641U (en) | 2016-08-14 | 2016-08-14 | Flow cytometry detecting tube temperature regulating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879345.3U CN205898641U (en) | 2016-08-14 | 2016-08-14 | Flow cytometry detecting tube temperature regulating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205898641U true CN205898641U (en) | 2017-01-18 |
Family
ID=57782083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620879345.3U Expired - Fee Related CN205898641U (en) | 2016-08-14 | 2016-08-14 | Flow cytometry detecting tube temperature regulating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205898641U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107044950A (en) * | 2017-03-19 | 2017-08-15 | 北京化工大学 | CD4+T lymphocyte counts detect micro fluidic device |
-
2016
- 2016-08-14 CN CN201620879345.3U patent/CN205898641U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107044950A (en) * | 2017-03-19 | 2017-08-15 | 北京化工大学 | CD4+T lymphocyte counts detect micro fluidic device |
| CN107044950B (en) * | 2017-03-19 | 2019-10-18 | 北京化工大学 | CD4+T lymphocyte count detects micro fluidic device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200730 Address after: Unit 601, block 1, No. 116, Gulou Road, Gulou District, Fuzhou City, Fujian Province Patentee after: Gui Jinpeng Address before: 100041 Beijing, Shijingshan District, West Wells Road, No. 19, building 6, floor 3, 601 Patentee before: BEIJING IVY BIOMED MEDICAL SCIENCE TECHNOLOGY Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170118 Termination date: 20200814 |