CN205844335U - Quickly detect the immune colloid gold test paper card of chylemia - Google Patents
Quickly detect the immune colloid gold test paper card of chylemia Download PDFInfo
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- CN205844335U CN205844335U CN201620603133.2U CN201620603133U CN205844335U CN 205844335 U CN205844335 U CN 205844335U CN 201620603133 U CN201620603133 U CN 201620603133U CN 205844335 U CN205844335 U CN 205844335U
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- gold
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- chylemia
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Abstract
A kind of immune colloid gold test paper card of quick detection chylemia, its colloidal gold strip includes support plate, NC film, gold labeling antibody pad, sample pad and adsorptive pads, NC film is arranged on the top of support plate, paste gold labeling antibody pad and sample pad on the support plate of NC film one end successively, the support plate of the other end pastes adsorptive pads；There are a detection line containing apolipoprotein B polyclonal antibody and a nature controlling line resisted containing IgG bis-in the middle part of NC film；This colloidal gold strip is arranged in the build-in cavities of buckle type draw-in groove, and makes detection line and the nature controlling line of the observation panel correspondence colloidal gold strip of buckle type draw-in groove；The well counter sample pad of buckle type draw-in groove.The utility model has the advantages that: with the method detection chylemia of gold colloidal, it is not necessary to using extra equipment, speed is fast, highly sensitive, when being particularly well-suited to street corner blood sampling, screens blood donor.The blood resource of blood donor can be protected, it is possible to save relevant detection, store, return the expenses such as blood expense.
Description
Technical field
This utility model belongs to clinical hematology detection, is specifically related to the immune colloid gold examination of a kind of quick detection chylemia
Paper card.
Background technology
Chylemia or title lipidemia, post prandial fat is through after little intestinal digestion, absorbing, and the Chylomicron becoming tiny enters blood
Liquid, Chylomicron many to a certain extent time, serum is just become milky thick liquid by as clear as crystal weak yellow liquid.Blood
Because of containing a large amount of triglyceride in slurry, and patient can not be defeated by.Triglyceride is the independent hazard factor of cardiovascular disease.
The most domestic when not taking a blood sample for street corner, quickly detect the way of content of triglyceride with peripheral blood, therefore cause adopting confession
Blood mechanism scraps substantial amounts of lipidemia every year, causes the huge waste of blood resource.
Apo B-48 (apoB-48) is unique by the lipoprotein of (people) intestinal secretion, and it is combined with triglyceride
Rear generation Chylomicron (chylomicrons CMs) and residue thereof.ApoB-48 in CM is structural protein, by apoB100
The special editor of mRNA and obtain.Apo B48 has 2153 aminoacid.After it is esterified by neutral fat, in triglyceride transport protein
Under the assistance of (triglyceride transfer protein (MTP)), generate the CM granule at initial stage.Unconjugated apoB48
Degrade immediately.Different from liver secretion very low density lipoprotein (VLDL) (VLDL), the apoB48 that degrades in enterocyte is not its main tune
Control mechanism, when lipid lacks, the apoB48 of enterocyte secretion is a kind of high density lipoprotein.ApoB48 in serum
Concentration can represent the granule number of CM and CM residue.Available apoB48 follows the trail of the metabolic process of intestinal secretion CM.
Immune colloidal gold technique is the optimum selection of rapid clinical detection.Enzyme-linked immunosorbent assay (ELISA), molecule are raw
Thing experimental technique is applicable to laboratory and uses.When not taking a blood sample for street corner, quickly detect with peripheral blood
The way of chylemia.Therefore cause Blood Transfusion Services to scrap substantial amounts of chylemia every year, cause the huge waste of blood resource.
Summary of the invention
When the purpose of this utility model is for solving street corner blood sampling, the problem of rapid screening chylemia.
This utility model be the technical scheme is that exempting from of a kind of quick detection chylemia by the above-mentioned technical problem of solution
Epidemic disease colloidal gold test paper card, it is characterised in that include colloidal gold strip and draw-in groove, this draw-in groove has an accommodating colloid gold test paper
The build-in cavities of bar, and on this draw-in groove, it is provided with observation panel and well；This colloidal gold strip is mainly by support plate, NC
Film, gold labeling antibody pad, sample pad and adsorptive pads composition, pass through bond samples successively in the side of this support plate
Pad, gold labeling antibody pad, NC film and adsorptive pads；A detection containing apolipoprotein polyclonal antibody it is provided with at the middle part of this NC film
Line and one are arranged with nature controlling line interval containing two anti-nature controlling lines, this detection line, and it is adjacent with golden labeling antibody pad to detect line；
This colloidal gold strip is arranged in the build-in cavities of draw-in groove, and makes detection line and the matter of this observation panel correspondence colloidal gold strip
Control line, well counter sample pad.
Described gold labeling antibody pad scribbles mouse-anti human apolipoprotein b 48 monoclonal antibody of colloid gold label.
Described detection line is rabbit anti-Mus apolipoprotein B polyclonal antibody；Described nature controlling line is that rabbit anti-mouse igg two resists.
Described detection line and the line width of nature controlling line are 1mm, and two lines are parallel is positioned at the centre of NC film at a distance of 3 ~ 5mm, away from
Distance 6 ~ the 7mm at NC film two ends.
The two ends of described NC film are overlapped with the abutting end of gold labeling antibody pad and adsorptive pads, gold labeling antibody pad and water suction
The lap of pad is pressed in above NC film two ends；This sample pad has overlapping with the abutting end of gold labeling antibody pad, this sample pad
Lap is pressed in above gold labeling antibody pad abutting end.
Described draw-in groove is buckle type draw-in groove.
The utility model has the advantages that: with the method detection chylemia of gold colloidal, it is not necessary to use extra equipment, speed
Hurry up, highly sensitive, when being particularly well-suited to street corner blood sampling, blood donor is screened.The blood resource of blood donor can be protected, also
Relevant detection can be saved, store, return the expenses such as blood expense.
Accompanying drawing explanation
Fig. 1 is immunity colloidal gold test paper strip structural representation of the present utility model；
Fig. 2 is the perspective view of this utility model buckle type draw-in groove；
Fig. 3 (a-d) is to use testing result schematic diagram of the present utility model.
Description of reference numerals: 1, sample pad；2, gold labeling antibody pad；3, NC film (nitrocellulose filter)；4, detection line, 5, matter
Control line；6, adsorptive pads；7, support plate；8, draw-in groove；9, observation panel；10. well.
Detailed description of the invention
Seeing Fig. 1 and Fig. 2, the immune colloid gold test paper card of a kind of quick detection chylemia of this utility model, its feature exists
In, including colloidal gold strip A and buckle type draw-in groove 8, this buckle type draw-in groove 8 has the interior of an accommodating colloidal gold strip A
It is empty chamber 11, and on this buckle type draw-in groove 8, is provided with observation panel 9 and well 10；This colloidal gold strip A is mainly by supporting
Plate 7, NC film 3, gold labeling antibody pad 2, sample pad 1 and adsorptive pads 6 form, and passing through in the side of this support plate 7 depends on
Secondary bond samples pad 1, gold labeling antibody pad 2, NC film 3 and adsorptive pads 6；One it is provided with containing apolipoprotein B at the middle part of this NC film 3
Detection line 4 and a nature controlling line 5 resisted containing IgG bis-, this detection line 4 and nature controlling line 5 interval of polyclonal antibody are arranged, and
Detection line 4 is adjacent with gold labeling antibody pad 2；This colloidal gold strip A is arranged in the build-in cavities 11 of buckle type draw-in groove 8, and makes
The region of the detection line 4 NC film 3 corresponding with nature controlling line 5(of the corresponding colloidal gold strip A of this observation panel 9), the corresponding glue of well 10
The sample pad 1 of body gold test paper strip.
In described gold labeling antibody pad 2, gold labeling antibody is the apolipoprotein B48 monoclonal antibody of colloid gold label.
Detection line 4 on described NC film 3 is apolipoprotein B polyclonal antibody, and nature controlling line 5 is that IgG bis-resists.
The preparation method of this utility model chylemia quick detection test paper card, comprises the following steps:
1) NC film 3 is prepared:
The preparation of detection line 4: use gold spraying instrument, with being coated buffer dilution carrier protein B polyclonal antibody, in NC film
Detection line position line, is detection line, dries standby；The described buffer that is coated is through 0.45 μm membrane filtration
The carbonate buffer solution of 0.05M pH9.6.
The preparation of nature controlling line 5: use gold spraying instrument, resists with being coated buffer dilution two, parallel with detection line on NC film
Ruling in position, is spaced 5mm, is nature controlling line, and the described buffer that is coated is the 0.05M pH9.6 through 0.45 μm membrane filtration
Carbonate buffer solution.
NC film assembles preparation: dry detection line and nature controlling line, obtains containing detection line and the NC film of nature controlling line；With closing work
Make liquid to be dried in room temperature 72h by the NC film of preparation, seal up for safekeeping standby；Described closing working solution be 2.5 ~ 3% BSA, 2 ~ 2.5% take off
Fat milk powder and 0.01M, obtain closing working solution with 0.45 μm membrane filtration after the carbonate buffer solution mixing of pH7.0.
2) preparation coating gold labeling antibody pad 2:
Gold target apolipoprotein B48 monoclonal antibody is diluted with the Trs-HCl buffer containing 1.5% BSA, 0.1% sodium azide；By gold
Labeling antibody solution gold spraying instrument, is uniformly sprayed on glass fibre cotton, 56 DEG C of dry 1h, after adding the sealing of desiccant vacuum, and room temperature
Save backup.
3) chylemia quick detection test paper card is assembled:
Detection line will be scribbled and the NC film of nature controlling line pastes the middle part at support plate, paste successively in the side of NC film containing
The glass fibre membrane of gold labeling antibody and sample pad, the opposite side at NC film is pasted adsorptive pads, is obtained test paper plate, cut by test paper plate
Become the test strips of different in width, test strips is placed in draw-in groove, is chylemia quick detection test paper card.
4) the using and judge of chylemia quick detection test paper card:
When the blood donor donating blood street corner carries out primary dcreening operation, gather terminal blood 40 microlitre of blood donor, drip in this reagent paper
In the sample pad 1 of card (in Fig. 1 at arrow B), after dropping blood, before serum will be by Fig. 1, arrow C direction carries out siphon
Enter, the apoB48 in serum first can with the apoB48 monoclonal antibody specific bond being marked with gold colloidal on gold mark pad 2, and
Travelling forward with serum, when arriving detection line 4 position, apoB48 can be fixed in the apoB48 polyclonal antibody of detection line 4
Being captured, when there being abundant apoB48 to be captured, the gold colloidal that it connects shows red because of gathering.Uncombined apoB48
The apoB48 monoclonal antibody being marked with gold colloidal may proceed to travel forward until nature controlling line 5, nature controlling line 5 is fixed with
IgG bis-resists, the two anti-apoB48 monoclonal antibodies being marked with gold colloidal of all of uncombined apoB48 that will capture, and aobvious red
Color.
When the detection line 4 of test card, nature controlling line 5 position all red stripes occur, chylemia test positive is (such as figure
3b)；When only a red stripes occurs in nature controlling line 5, for negative findings (such as Fig. 3 a)；Nature controlling line 5 at test card does not occurs red
It is null result (such as Fig. 3 c and Fig. 3 d) during vitta band.
Claims (6)
1. the immune colloid gold test paper card of a quick detection chylemia, it is characterised in that include colloidal gold strip and draw-in groove,
This draw-in groove has the build-in cavities of an accommodating colloidal gold strip, and is provided with observation panel and well on this draw-in groove；Should
Colloidal gold strip is mainly made up of, in the side of this support plate support plate, NC film, gold labeling antibody pad, sample pad and adsorptive pads
Pass through bond samples pad, gold labeling antibody pad, NC film and adsorptive pads successively；Be provided with at the middle part of this NC film one with
Detection line that apolipoprotein polyclonal antibody draws and one are spaced with nature controlling line set with the two anti-nature controlling lines drawn, this detection lines
Put, and it is adjacent with gold labeling antibody pad to detect line；This colloidal gold strip is arranged in the build-in cavities of draw-in groove, and makes this observation
The detection line of the corresponding colloidal gold strip of mouth and nature controlling line, well counter sample pad.
The immune colloid gold test paper card of quick detection chylemia the most according to claim 1, it is characterised in that
Described gold labeling antibody pad scribbles mouse-anti human apolipoprotein b 48 monoclonal antibody of colloid gold label.
The immune colloid gold test paper card of quick detection chylemia the most according to claim 1, it is characterised in that
Described detection line is rabbit anti-Mus apolipoprotein B polyclonal antibody；Described nature controlling line is that rabbit anti-mouse igg two resists.
The immune colloid gold test paper card of quick detection chylemia the most according to claim 1, it is characterised in that described inspection
The line width of survey line and nature controlling line is 1mm, and two lines are parallel at a distance of 3 ~ 5mm, are positioned at the centre of NC film, the distance 6 away from NC film two ends ~
7mm。
The immune colloid gold test paper card of quick detection chylemia the most according to claim 1, it is characterised in that described NC
The two ends of film are overlapped with the abutting end of gold labeling antibody pad and adsorptive pads, and the lap of gold labeling antibody pad and adsorptive pads is pressed in
Above NC film two ends；This sample pad has overlapping with the abutting end of gold labeling antibody pad, and the lap of this sample pad is pressed in gold mark
Above antibody pad abutting end.
The immune colloid gold test paper card of quick detection chylemia the most according to claim 1, it is characterised in that described card
Groove is buckle type draw-in groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620603133.2U CN205844335U (en) | 2016-06-20 | 2016-06-20 | Quickly detect the immune colloid gold test paper card of chylemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620603133.2U CN205844335U (en) | 2016-06-20 | 2016-06-20 | Quickly detect the immune colloid gold test paper card of chylemia |
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| Publication Number | Publication Date |
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| CN205844335U true CN205844335U (en) | 2016-12-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620603133.2U Expired - Fee Related CN205844335U (en) | 2016-06-20 | 2016-06-20 | Quickly detect the immune colloid gold test paper card of chylemia |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107132356A (en) * | 2017-04-27 | 2017-09-05 | 北京航空航天大学 | A kind of colloidal gold test paper card for detecting carcinoma of urinary bladder and preparation method thereof |
-
2016
- 2016-06-20 CN CN201620603133.2U patent/CN205844335U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107132356A (en) * | 2017-04-27 | 2017-09-05 | 北京航空航天大学 | A kind of colloidal gold test paper card for detecting carcinoma of urinary bladder and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161228 Termination date: 20170620 |