CN205786033U - The device of biotic component in a kind of collection and preservation urine - Google Patents
The device of biotic component in a kind of collection and preservation urine Download PDFInfo
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- CN205786033U CN205786033U CN201620533745.9U CN201620533745U CN205786033U CN 205786033 U CN205786033 U CN 205786033U CN 201620533745 U CN201620533745 U CN 201620533745U CN 205786033 U CN205786033 U CN 205786033U
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Abstract
The utility model discloses a kind of collection and the device of biotic component in preservation urine.Described device includes syringe and seals, with the syringe of described syringe, the filter coordinated；Radially being equipped with adsorbed film in described filter, described adsorbed film is the pvdf membrane of nitrocellulose filter, nylon membrane or activation；Described adsorbed film seals with the sidewall of described filter and coordinates.This utility model is injected by syringe, by biotic component absorption adsorbed film in filter in urine, film is dried, with biotic component in form short-term preservation urine airtight, that be dried.The method of biotic component in urine is collected and preserved to this utility model, and operation is simple, and patient can Self-operating；It is little that memory space shared by the device of biotic component in urine is collected and preserved to this utility model；The most important thing is that in urine, biotic component is the airtight preservation carrying out the short time when dry, thus avoid the degraded of biotic component；Therefore, this utility model is conducive to remote capture and the transport of urine specimen.
Description
Technical field
This utility model relates to a kind of collection and the device of biotic component in preservation urine.
Background technology
Biomarker refers to the change monitored relevant to physiology or pathophysiological process, and its essence is change.
Blood plasma, as the important component part of organismic internal environment, is regulated various physical and chemical compositions by homeostatic mechanism and keeps relative constancy,
I.e. excursion is little, it is few to accommodate biomarker；And urine is as the metabolic waste of body, comprise more reflection body
The information of change, the most biomarkers.I.e. urine is probably the source preferably excavating biomarker.
Along with the proposition of accurate medical concept, biological sample bank (Biobank) plays the most important role.Urine
As important biological specimen, if it together can be preserved in each stage of disease with clinical case history, then
Find in the retrospective of biomarker and perspective study in future, particularly in larger scale clinical confirmatory experiment,
Immeasurable effect will be played.Especially those are scattered and live in the patient of all parts of the country, for the urine of these crowds
The collection of liquid sample faces storage and the difficulty of transport.
Utility model content
The purpose of this utility model is to provide a kind of collection and the device of biotic component in preservation urine, this utility model energy
Biotic component in enough short-term preservation urines simply, consequently facilitating remote capture, transport urine specimen.
Collection provided by the utility model and preserve the device of biotic component in urine, including syringe and with described note
The syringe of emitter seals the filter coordinated；
Radially being equipped with adsorbed film in described filter, described adsorbed film is nitrocellulose filter, nylon membrane or activation
Pvdf membrane；
Described activation refers to soak in several seconds pvdf membrane in methanol；
Described adsorbed film seals with the sidewall of described filter and coordinates.
In above-mentioned device, described adsorbed film is also laid isolating membrane.
In above-mentioned device, described isolating membrane is pvdf membrane.
In above-mentioned device, the aperture of described isolating membrane is 0.22 μm～0.45 μm, concretely 0.22 μm or 0.45 μm.
In above-mentioned device, described isolating membrane is one layer.
In above-mentioned device, the aperture of described nitrocellulose filter is 0.22 μm～0.45 μm, concretely 0.22 μm
Or 0.45 μm；
The aperture of described nylon membrane is 0.22 μm～0.45 μm, concretely 0.22 μm or 0.45 μm.
In above-mentioned device, described nitrocellulose filter is one layer；
Described nylon membrane is one layer.
In above-mentioned device, the cross section of described filter is circular.
When utilizing this utility model device be collected and preserve biotic component in urine, can carry out in accordance with the following steps:
Under pressure, make urine accepted filtered absorption film, then the biotic component in urine is adsorbed on described adsorbed film,
I.e. realize collection and the preservation of biotic component in urine.
In above-mentioned method, the effect of isolating membrane is, when urine passes through, described isolating membrane will not be in conjunction with in urine
Biotic component, and intercept cell in urine and cell debris and contact combination with adsorbed film.
In above-mentioned method, described urine is sucked in syringe, makes described under the thrust of described syringe
Urine filters described adsorbed film.
In above-mentioned method, after the described urine in described syringe all filters described adsorbed film, described method is also
Comprise the steps: that described syringe is drawn air and injects and be run through described adsorbed film.
In above-mentioned method, described method also includes being dried having adsorbed the described adsorbed film of biotic component in urine
Step, natural drying can be carried out or use the mode that dries up of hair-dryer.
Adsorbed film through being dried, desiccant, label paper are placed in airtight valve bag and carry out short-term preservation.Then
Being taken out by adsorbed film and be saved in vacuum bag, labelled paper, room temperature continues to deposit or low-temp storage.
This utility model is injected by syringe, by biotic component absorption adsorbed film in filter in urine, by film
After drying, with biotic component in form short-term preservation urine airtight, that be dried.
The method of biotic component in urine is collected and preserved to this utility model, and operation is simple, and patient can Self-operating；
It is little that memory space shared by the device of biotic component in urine is collected and preserved to this utility model；The most important thing is in urine
Biotic component is the airtight preservation carrying out the short time when dry, thus avoids the degraded of biotic component；Cause
This, the present invention is conducive to remote capture and the transport of urine specimen.
Accompanying drawing explanation
Fig. 1 is that the structural representation of the device of biotic component in urine is collected and preserved to this utility model；
In figure, each labelling is as follows: 1 syringe, 2 filters.
Fig. 2 is to collect and preserve in urine urine collecting process in the method for biotic component in this utility model embodiment 2
Schematic diagram.
Fig. 3 is the SDS-PAGE analysis chart of protein in urine in this utility model embodiment 4.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described further, but this utility model is not limited to following example.
Embodiment 1, collect and preserve the device of biotic component in urine
In the collection of this utility model offer and preservation urine, the structural representation of the device of biotic component is as shown in Figure 1.
As it is shown in figure 1, this utility model device includes syringe 1 and circular filter 2, the syringe of syringe 1 with
The arrival end of filter seals and coordinates.In filter 2, radially direction is equipped with one layer of adsorbed film and the most successively
Layer isolating membrane (not shown), adsorbed film is for adsorbing the biotic component in urine, and isolating membrane will not be in conjunction with in urine
Biotic component, and intercept cell in urine and cell debris and contact combination with adsorbed film.Adsorbed film is according to being received
The biotic component of collection selects, such as the nitrocellulose filter of adsorbed proteins, the nylon membrane of absorption nucleic acid.Nitric acid is fine
The aperture of dimension element film is 0.22 μm, and the aperture of nylon membrane is 0.22 μm.Isolating membrane is chosen as pvdf membrane, its aperture
It it is 0.22 μm.
Embodiment 2, utilize the protein that device in embodiment 1 collects and preserve in urine
In the present embodiment, the process schematic of urine collecting is as shown in Figure 2.
The device utilizing embodiment 1 to provide carries out the collection of protein in urine, specifically comprises the following steps that
1, Healthy People mixing urine 400ml (1 male 1 female) is collected.
2, place nitrocellulose filter one layer in filter 2, it places one layer of pvdf membrane again.
3,20ml urine is drawn with syringe 1, syringe 1 is closely coupled with filter 2 top inlet, slowly push away
Dynamic syringe stem, in making syringe 1, urine is completely by the nitrocellulose filter in filter 2.Again in syringe 1
Draw a pipe air, by closely coupled with filter 2 top inlet for syringe 1, slowly pushing syringe bar, make injection
In device, air passes through filter completely.
4, to oozing from filter lower exit port without urine, take filter apart, take out nitrocellulose filter, naturally dry in room temperature
Dry.
5, by nitrocellulose filter, desiccant, same to label paper (recorded content: medical record number, take urine the date, take urine time
Between, medicining condition etc.) put into airtight room temperature preservation in valve bag.
In the urine preserved on the present embodiment nitrocellulose filter, the extraction process of protein is as follows:
1, the nitrocellulose membrane of adsorbing urine protein matter is shredded it is placed in 2mL centrifuge tube, be sequentially added into 1.7mL acetone,
0.2mL 0.5% ammonium bicarbonate aqueous solution.
2, by mixture room temperature intense oscillations 20s, it is placed in 55 DEG C of dry bath device 60min, and stops adding every 20min
Hot intense oscillations 30s.
3,4 DEG C of jog 2h, 12 000r/min, 18 DEG C of centrifugal 15min, abandon supernatant, and room temperature places 5-10min,
Dry.
4,150 μ L lysis buffers (7M carbamide, 2M thiourea, 40Mm Tris, 25mM bis-sulfur threose are added
Alcohol), ultrasonic 3min after piping and druming.
5,12 000r/min, 18 DEG C of centrifugal 15min, take supernatant.
6, after Bradford method surveys protein concentration ,-80 DEG C frozen standby.
Embodiment 3, nitrocellulose filter preserve the reperformance test of urine albumen reserve capacity
According to the step in embodiment 2, by the 20ml urine filter of mixing, protein is stored in celluloid
On film, it is repeated 18 times.Wherein 6 gained nitrocellulose filters directly-80 DEG C frozen, 6 gained celluloid
Film room temperature preservation 3 days, other 6 gained nitrocellulose filter room temperature preservation 7 days.By dry nitrocellulose filter
Shredding, extract the protein preserved on nitrocellulose filter according to the extracting method in embodiment 2, Branford method is surveyed
Protein concentration, and calculate total amount.
Result is as shown in table 1-table 3, and result shows, by the most frozen for the nitrocellulose filter being adsorbed with urine protein,
The mean+SD of the total protein obtained is 163.40 ± 33.47, and the coefficient of variation is 0.2048；Room temperature preservation
3 days, the mean+SD of the total protein obtained was 93.88 ± 29.49, and the coefficient of variation is 0.3141；Room
Temperature preserves 7 days, and the mean+SD of the total protein obtained is 89.281 ± 10.895, and the coefficient of variation is
0.1220。
Total protein in urine in 1-80 DEG C of frozen celluloid of table
Total protein in urine in the table 2 room temperature preservation celluloid of 3 days
Total protein in urine in the table 3 room temperature preservation celluloid of 7 days
Embodiment 4, SDS-polyacrylamide gels analyze protein (nitric acid in the urine extracted in embodiment 2
Cellulose membrane preserves urine albumen and preserves the repeatability of molecular weight)
The protein preserved in embodiment 3 is analyzed as follows:
Urine protein sample adds 5 × loading buffer, and 97 DEG C are boiled 10min.Being cooled to room temperature, loading 25 μ g urinates
Liquid eggs is white.Separation gel 4%～12%, 200v, 45min.Coomassie brilliant blue staining, decolouring overnight, shoots photo.
As it is shown on figure 3, Fig. 3 shows, the band of SDS-PAGE is the sharpest keen, without obvious degradation.Through room temperature 3
My god, the urine protein sample that preserves for 7 days, compared with the most frozen urine protein sample, cover absolutely big portion
Divide same strap, there is good repeatability.
Claims (7)
1. collect and preserve the device of biotic component in urine for one kind, it is characterised in that: described device includes syringe
And seal, with the syringe of described syringe, the filter coordinated；
Radially being equipped with adsorbed film in described filter, described adsorbed film is nitrocellulose filter, nylon membrane or activation
Pvdf membrane；
Described adsorbed film seals with the sidewall of described filter and coordinates.
Device the most according to claim 1, it is characterised in that: also lay isolating membrane on described adsorbed film.
Device the most according to claim 2, it is characterised in that: described isolating membrane is pvdf membrane.
Device the most according to claim 3, it is characterised in that: the aperture of described isolating membrane is
0.22 μm～0.45 μm；
Described isolating membrane is one layer.
5. according to the device according to any one of claim 1-4, it is characterised in that: the hole of described nitrocellulose filter
Footpath is 0.22 μm～0.45 μm；
Described nitrocellulose filter is one layer.
6. according to the device according to any one of claim 1-4, it is characterised in that: the aperture of described nylon membrane is
0.22 μm～0.45 μm；
Described nylon membrane is one layer.
7. according to the device according to any one of claim 1-4, it is characterised in that: the cross section of described filter is circle
Shape.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620533745.9U CN205786033U (en) | 2016-06-02 | 2016-06-02 | The device of biotic component in a kind of collection and preservation urine |
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| CN201620533745.9U CN205786033U (en) | 2016-06-02 | 2016-06-02 | The device of biotic component in a kind of collection and preservation urine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053193A (en) * | 2016-06-02 | 2016-10-26 | 北京师范大学 | Method and device for collecting and storing biological components in urine by filter |
| CN108344620A (en) * | 2017-01-23 | 2018-07-31 | 北京师范大学 | A kind of method and its device concentrated and preserve genomic DNA in urine specimen |
-
2016
- 2016-06-02 CN CN201620533745.9U patent/CN205786033U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053193A (en) * | 2016-06-02 | 2016-10-26 | 北京师范大学 | Method and device for collecting and storing biological components in urine by filter |
| CN108344620A (en) * | 2017-01-23 | 2018-07-31 | 北京师范大学 | A kind of method and its device concentrated and preserve genomic DNA in urine specimen |
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