CN205720860U - A kind of AFM and super-resolution fluorescence microscope are combined Imaged samples dish - Google Patents
A kind of AFM and super-resolution fluorescence microscope are combined Imaged samples dish Download PDFInfo
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- CN205720860U CN205720860U CN201620251187.7U CN201620251187U CN205720860U CN 205720860 U CN205720860 U CN 205720860U CN 201620251187 U CN201620251187 U CN 201620251187U CN 205720860 U CN205720860 U CN 205720860U
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Abstract
The utility model provides a kind of AFM and super-resolution fluorescence microscope to be combined Imaged samples dish, belongs to chemical industry Instrument technology field.Solve the problem that existing sample disc can not meet two kinds of Image-forming instruments simultaneously.This sample disc includes sample disc body, and described sample disc body central arranges a circular hole, and the diameter of described circular hole is more than the diameter of super-resolution fluorescence microscope camera lens.It this sample disc simple in construction, AFM and super-resolution fluorescence microscope can be completed simultaneously is combined imaging；In use, two kinds of imaging modes do not interfere with each other, and solve the problem simultaneously gathering same fluorescent image and atomic force image, have reached the purpose to sample surface morphology and target molecule positioning analysis.
Description
Technical field
The utility model relates to chemical industry Instrument technology field, and concrete a kind of AFM is combined Imaged samples dish with super-resolution fluorescence microscope.
Background technology
AFM (atomic force microscopy, AFM), is the important a member in scanning probe microscopy family.AFM is connected to, by detection, the various information that interaction force faint between the small probe of elastic micro-cantilever one end and sample obtains sample surfaces.When probe is close to sample surface, probe is made the micro-cantilever of linking probe deflect (contact mode) by the attraction of sample surfaces or amplitude changes (tapping-mode).Utilize detecting system to be converted into the signal of telecommunication and pass to computer generated image system this skew of probe cantilevers or the change of amplitude, thus obtain the information of sample surfaces.Different from light microscope and electron microscope, AFM has surmounted the restriction to microscope highest resolution of light wave and electron wavelength, can obtain the three-dimensional stereo topography of material under vacuum, air and liquid environment.The lateral resolution of AFM can reach nanoscale, and vertical resolution can reach 0.1nm.The remarkable AFM of function has been applied to each science and technology field, whether still all presents it in terms of the morphology analysis of liver cell surface in surface of base science and is widely applied prospect.
The invention of super-resolution fluorescence microtechnic breaches diffraction limit so that resolution ratio can reach tens nanometers, thus can observe on single molecules level well and research biological process.Super-resolution imaging method based on unimolecule positioning, as random optical rebuilds microscope (STORM), photoactivation position finding microscope (PALM) etc., in biological study, play more and more important effect with superiority such as the resolution capability of its brilliance, relatively simple devices.The super-resolution imaging method of STORM and PALM has the steps: first using can be at the fluorescent dye of fluorescence state/dark-state conversion or fluorescent protein labeling sample, at any time, in imaging process, fluorescence probe in most of samples is maintained at dark-state, and only random sub-fraction is converted to fluorescence state；Fluorescence probe luminous afterwards is changed by light or photobleaching is dark-state；Another fraction fluorescence probe is fluorescence state by random transition subsequently；And so forth so that the labeled independent imaging of molecule；Finally all of imaging is accumulated, finally reconstruct super-resolution imaging.
Technology in conjunction with two kinds of microscopical imagings can strengthen the advantage of micro-imaging.But microscopical for both imaging advantage can not be brought into play by simply instrument combination.Microscopical manufacture commercial city uses oneself production sample imaging device, and these devices can not be compatible with other microscopes.More specifically, AFM is different from the image-forming principle of super-resolution imaging, and single imaging device can not meet the microscopical imaging demand of both simultaneously.
Utility model content
The purpose of this utility model is the problem that can not simultaneously meet two kinds of Image-forming instruments in order to solve existing sample disc, and provides a kind of AFM and super-resolution fluorescence microscope to be combined Imaged samples dish.
The utility model provides a kind of AFM and super-resolution fluorescence microscope to be combined Imaged samples dish, this sample disc includes sample disc body, described sample disc body central arranges a circular hole, and the diameter of described circular hole is more than the diameter of super-resolution fluorescence microscope camera lens.
The beneficial effects of the utility model
The utility model provides a kind of AFM and super-resolution fluorescence microscope to be combined Imaged samples dish, this sample disc includes sample disc body, described sample disc body central arranges a circular hole, and the diameter of described circular hole is more than the diameter of super-resolution fluorescence microscope camera lens.It this sample disc simple in construction, AFM and super-resolution fluorescence microscope can be completed simultaneously is combined imaging；In use, two kinds of imaging modes do not interfere with each other, and solve the problem simultaneously gathering same fluorescent image and atomic force image, have reached the purpose to sample surface morphology and target molecule positioning analysis.
Brief description
Fig. 1 is the structural representation of a kind of AFM of the utility model and super-resolution fluorescence microscope combination Imaged samples dish.
Fig. 2 is the structural representation of a kind of AFM of the utility model and super-resolution fluorescence microscope combination Imaged samples dish.
In figure, the 1st, sample disc body, the 2nd, bolt hole, the 3rd, position hole A, the 4th, circular hole, the 5th, position hole B, the 6th, spring clip.
Detailed description of the invention
Below in conjunction with the accompanying drawings further detailed description is done to the utility model.
As depicted in figs. 1 and 2, a kind of AFM and super-resolution fluorescence microscope are combined Imaged samples dish, this sample disc includes sample disc body 1, the standard sample dish body that described sample disc body 1 is AFM, described sample disc body central arranges a circular hole 4, and the diameter of described circular hole 4 is more than the diameter of super-resolution fluorescence microscope camera lens.
Depending on the diameter according to super-resolution fluorescence microscope camera lens for circular hole 4 diameter described in present embodiment, the preferably a diameter of 20mm of circular hole 4, the diameter of super-resolution fluorescence microscope camera lens is preferably 18mm, and the microscopical camera lens of super-resolution fluorescence can contact with sample through circular hole 4 and complete super-resolution imaging.
In order to fix sample disc, sample disc body 1 arranges two bolts hole 2 or spring clip 6, described bolt hole 2 or spring clip 6 are symmetricly set on the both sides of circular hole 4, when carrying out the imaging of fluid sample, by spring clip 6, liquid Imaged samples pond can be fixed, complete the IMAQ of sample under liquid phase.
1 positioning hole A3 and 3 positioning hole B5 are also set up in order to position sample disc on sample disc body 1 described in present embodiment.
When carrying out two kinds of Instrument crosslinking imagings, sample is arranged on the cover glass of a diameter of 22mm, it is positioned over sample disc centre position, can directly imaging above sample prepared by cover glass during AFM imaging, when carrying out fluorescence imaging, camera lens can complete to obtain same sample the purpose of two kinds of images by circular hole 4 to sample imaging.
Claims (4)
1. an AFM is combined Imaged samples dish with super-resolution fluorescence microscope, it is characterized in that, this sample disc includes sample disc body (1), the centrally disposed circular hole (4) of described sample disc body (1), the diameter of described circular hole (4) is more than the diameter of super-resolution fluorescence microscope camera lens.
2. a kind of AFM according to claim 1 is combined Imaged samples dish with super-resolution fluorescence microscope, it is characterised in that the described a diameter of 20mm of circular hole (4), a diameter of 18mm of super-resolution fluorescence microscope camera lens.
3. a kind of AFM according to claim 1 is combined Imaged samples dish with super-resolution fluorescence microscope, it is characterized in that, described sample disc also includes two bolts hole (2), and two described bolts hole (2) are symmetricly set on the both sides of circular hole (4).
4. a kind of AFM according to claim 1 is combined Imaged samples dish with super-resolution fluorescence microscope, it is characterized in that, described sample disc also includes two spring clips (6), and two described spring clips (6) are symmetricly set on the both sides of circular hole (4).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620251187.7U CN205720860U (en) | 2016-03-29 | 2016-03-29 | A kind of AFM and super-resolution fluorescence microscope are combined Imaged samples dish |
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| CN201620251187.7U CN205720860U (en) | 2016-03-29 | 2016-03-29 | A kind of AFM and super-resolution fluorescence microscope are combined Imaged samples dish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918580A (en) * | 2017-02-22 | 2017-07-04 | 大连光耀辉科技有限公司 | A kind of super-resolution fluorescence microscopic system with nanometer infrared imaging function |
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2016
- 2016-03-29 CN CN201620251187.7U patent/CN205720860U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918580A (en) * | 2017-02-22 | 2017-07-04 | 大连光耀辉科技有限公司 | A kind of super-resolution fluorescence microscopic system with nanometer infrared imaging function |
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