CN205643151U - Portable hydrogen peroxide detector - Google Patents
Portable hydrogen peroxide detector Download PDFInfo
- Publication number
- CN205643151U CN205643151U CN201620465528.0U CN201620465528U CN205643151U CN 205643151 U CN205643151 U CN 205643151U CN 201620465528 U CN201620465528 U CN 201620465528U CN 205643151 U CN205643151 U CN 205643151U
- Authority
- CN
- China
- Prior art keywords
- hydrogen peroxide
- metal module
- colorimetric pool
- photoelectric detector
- signal input
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model provides a portable hydrogen peroxide detector, relates to a hydrogen peroxide detecting instrument's improvement, characterized by: be fixed with metal module at colorimetric pool thermotank central part, cylindricality groove and colorimetric pool clearance fit, metal module is arranged in to the colorimetric pool, is equipped with the electronic heating area in the metal module, and the LED monochromatic point source sets up in metal module peripherally with the photoelectric detector symmetry, the photoelectric detector output is connected with the amplifier signal input, and the amplifier signal output is connected with AD analog to digital conversion circuit signal input part, and AD analog to digital conversion circuit signal output part and microprocessor signal input part are connected, and microprocessor amplifier signal 0 end is connected with the electronic heating area. The beneficial effects are that: do you utilize dhHP 6 constitute hydrogen peroxide photometry's reagent, establish quick, a simple and convenient hydrogen peroxide measuring method. The instrument have constant temperature, regularly, functions such as liquid crystal display, printing, small, light in weight, portable's characteristics in addition simultaneously.
Description
Technical field
This utility model belongs to biology, medical science, food, environment measuring equipment technical field, relates to the improvement of a kind of hydrogen peroxide detecting instrument.
Background technology
Hydrogen peroxide (H2O2) be widely present in biosphere, it participates in pathology and the physiological process such as oxidation, immunologic function and the tumor in body.H2O2Mensuration in the detection and relevant clinical practice of biological specimen, have an important meaning, the detection of many important physical signs (glucose and uric acid etc.) is to utilize to generate respective amount hydrogen peroxide and calculate.Additionally, food and Environmental safety supervision the most often measure H2O2, therefore, timely, special, Sensitive Detection H2O2Medical diagnosis on disease, food safety and environmental monitoring etc. are had great importance.
At present, the analysis method of detection concentration of hydrogen peroxide mainly has: titrimetry, spectrophotography, fluorimetry, luminescence method, chromatography, electrochemical methods and enzyme chemical method etc..Wherein, the iodimetric titration in titrimetry is to be most widely used for detecting the method for content of hydrogen peroxide in bleaching process in manufacturing enterprise's work, although the method is simple to operate, but interference factor is many, sensitivity is low.Enzyme chemical method has advantage highly sensitive, that specificity good and detection limit is low, but peroxidase (POD) price needed in measuring is high, easy in inactivation degeneration.Although other use Modern Analytical Instrument to analyze the method for content of hydrogen peroxide, it is highly sensitive, can operate continuously, but due to its apparatus expensive, operation complexity, it is impossible to realize easy, quickly detect, lack popularity and the general applicability of application.
Summary of the invention
The purpose of this utility model is: provide a kind of portable hydrogen peroxide detector, it utilizes secondary heme hexapeptide (DhHP-6) to have the function of mimichydrogen peroxidase, the reagent of composition hydrogen peroxide photometry, designs a kind of quick, easy detecting instrument quantitative determining hydrogen peroxide in fluid sample.
The technical solution of the utility model is: be made up of light source, colorimetric pool, photoelectric detector, signal amplifier, A/D change-over circuit, temperature-control circuit, microprocessor and display, printer etc..
The metal module of band cylindrical groove it is fixed with at colorimetric pool temperature chamber central part, cylindrical groove and colorimetric pool matched in clearance, colorimetric pool is placed in metal module, electronics heating tape it is provided with in metal module, it is peripheral that LED monochromater and photoelectric detector are symmetricly set in metal module, and makes LED monochromatic light arrive photoelectric detector through colorimetric pool vertical center line.
Photoelectric detector outfan is connected with amplifier signal input, amplifier signal outfan is connected with A/D analog to digital conversion circuit signal input part, A/D analog to digital conversion circuit signal output part is connected with microprocessor signals input, and temperature of micro controls end and is connected with electronics heating tape.
The beneficial effects of the utility model are: it will overcome problem present at present conventional hydrogen peroxide detection method, DhHP-6 is utilized to have the function of mimichydrogen peroxidase, the reagent of composition hydrogen peroxide photometry, sets up a kind of quick, easy hydrogen peroxide determination method.Instrument have fixing wavelength (505nm), constant temperature, regularly, liquid crystal display, the function such as printing, the most also volume feature little, lightweight, portable.
Accompanying drawing explanation
Fig. 1 is this utility model theory diagram；
Fig. 2 is this utility model internal structure schematic diagram；
Fig. 3 is this utility model side view；
Fig. 4 is this utility model colorimetric pool cut-away view
Fig. 5 is that this utility model measures variable concentrations H2O2Linear graph.
Detailed description of the invention
Embodiment 1
As shown in Figure 2,3, 4,1 is the casing of portable hydrogen peroxide detector；2 is electronic display；3 is colorimetric basin lid；4 is to arrange select button；5 is printer.
As shown in Figure 4,3-1 is LED light source；3-2 is colorimetric pool temperature chamber；3-3 is colorimetric pool temperature chamber；3-4 is photoelectric detector.
Casing 1 is made up of inside and outside double-layer structure, and inside and outside layer is metal material, has insulation material between ectonexine, and it provides keeping warmth space for Portable type automatic constant-temperature device.Electronic display 2, can be configured the time (year, month, day, hour, min), (30～56 DEG C select thermostatic mode, constant temperature time, real-time digital temperature shows and detect program setting and hydrogen peroxide testing result thermostat temperature.Colorimetric basin lid 3, it is made up of metal material, above temperature chamber, has the effect of insulation and lucifuge.It is placed and taken out during colorimetric pool opening groove lid, when sample constant temperature and colorimetric, covers groove lid.There is metal module (3-2) in colorimetric pool, be responsible for providing temperature to colorimetric pool.Arrange select button 4 be responsible for temperature set, regularly, concentration of standard solution, computing formula set.The output of measurement result is responsible for by printer 5.On and off switch 6 it provide electric power for instrument.
Light source uses LED monochromatic light, has deducted the monochromator (optical filter or grating etc.) in conventional analytical tool.Colorimetric pool is placed in metal module, and electronics heating tape controls the temperature of metal module at 37 DEG C.LED monochromatic light arrives photoelectric detector receiving terminal by being placed in the colorimetric pool of constant temperature in metal module, signal is passed to amplifier receiving terminal by photoelectric detector outfan, shaped, amplification, signal is passed to A/D change-over circuit receiving terminal by amplifier out, signal is passed to microprocessor by A/D change-over circuit outfan, it is calculated testing result by microprocessor, output end of microprocessor 1 measurement result is delivered to display or measurement result is delivered to printer by outfan 2.Theory diagram is shown in Fig. 1.
Operating process
1, before sample mensuration to be carried out, portable hydrogen peroxide detector casing (1) power supply (6) is connected.
2, electronic display (2) main screen display current time and temperature, can be chosen as undesirable, be adjusted time and temperature on a display screen；Colorimetric pool temperature chamber (3-2) starts to heat, and reaches desired temperature after a period of time (depending on ambient temperature).Constant-temperature incubation time, Hydrogen peroxide standard liquid concentration are set.After selection completes, return electron display screen (2) main screen, now main screen is by real-time display digit temperature.
3, after the digital temperature shown when screen (2) reaches predetermined temperature, open colorimetric basin lid (3), be intended to colorimetric reagent and put into colorimetric pool (3-3) groove, close colorimetric basin lid (3).
4, after reaching constant temperature time, alarm sound can be had to point out, reach the temperature of constant temperature simultaneously in the upper display of main screen (2)；
5, open colorimetric basin lid (3), in colorimetric reagent, add Hydrogen peroxide standard liquid to be measured, be intended to colorimetric reagent and put into colorimetric pool groove (3-3), close colorimetric basin lid (3).LED light source (3-1) is luminous, reaches photoelectric detector (3-4) by colorimetric pool (3-3) and measures initial absorbance (OD1A), after reaching constant temperature time, measure and terminate absorbance, main screen (2) upper display Hydrogen peroxide standard liquid absorbance (OD2A), store.△(OD)Standard= OD2A- OD1A
6, in colorimetric reagent, add sample to be measured, measure initial absorbance (OD1B), after reaching constant temperature time, measure and terminate absorbance (OD2B), upper display sample the to be measured absorbance of main screen (2) and concentration of hydrogen peroxide.△(OD)Sample= OD2B- OD1B。
H2O2(mmol/L)=△(OD)Sample/△(OD)Standard×C H2O2 Standard concentration
7, selecting to print, hydrogen peroxide determination result is printed by printer (5) automatically.
(1) reagent
1, DhHP-6 reagent
2,3% hydrogen peroxide,
(2) experimental technique
Measure H2O2Experimental procedure:
(1), DhHP-6 reagent there is peroxidase function, H can be caused2O2
Decompose and produce O-.Therefore the H of variable concentrations can be used2O2Carry out laboratory observation.
(2), by 3.0% H2O2Be diluted to concentration be 22.0,11.0,5.50,2.75,1.375,0.687mmol/L standby.
(3) distilled water, DhHP-6 reagent variable concentrations H, are taken respectively2O2Solution.Operating by table 1,37 DEG C measured absorbance (OD) at 10 minutes respectively on portable hydrogen peroxide detector.Measure the linear of variable concentrations H2O2, Fig. 4.
H2O2The experimental result measured shows, portable hydrogen peroxide detector measures variable concentrations H2O2There are preferable repeatability, linear and stability.
Claims (1)
1. a portable hydrogen peroxide detector, it is characterized in that: be fixed with the metal module of band cylindrical groove at colorimetric pool temperature chamber central part, cylindrical groove and colorimetric pool matched in clearance, colorimetric pool is placed in metal module, electronics heating tape it is provided with in metal module, it is peripheral that LED monochromater and photoelectric detector are symmetricly set in metal module, and makes LED monochromatic light arrive photoelectric detector through colorimetric pool vertical center line；Photoelectric detector outfan is connected with amplifier signal input, amplifier signal outfan is connected with A/D analog to digital conversion circuit signal input part, A/D analog to digital conversion circuit signal output part is connected with microprocessor signals input, and temperature of micro controls end and is connected with electronics heating tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620465528.0U CN205643151U (en) | 2016-05-23 | 2016-05-23 | Portable hydrogen peroxide detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620465528.0U CN205643151U (en) | 2016-05-23 | 2016-05-23 | Portable hydrogen peroxide detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205643151U true CN205643151U (en) | 2016-10-12 |
Family
ID=57054192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620465528.0U Expired - Fee Related CN205643151U (en) | 2016-05-23 | 2016-05-23 | Portable hydrogen peroxide detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205643151U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109507176A (en) * | 2018-11-12 | 2019-03-22 | 长春理工大学 | Dry chemical hydrogen peroxide detection method based on secondary heme hexapeptide |
-
2016
- 2016-05-23 CN CN201620465528.0U patent/CN205643151U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109507176A (en) * | 2018-11-12 | 2019-03-22 | 长春理工大学 | Dry chemical hydrogen peroxide detection method based on secondary heme hexapeptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wu et al. | Clinical chemistry measurements with commercially available test slides on a smartphone platform: Colorimetric determination of glucose and urea | |
| US20100259394A1 (en) | Mobile communication terminal equipped with temperature compensation function for use in bio-information measurement | |
| KR101526217B1 (en) | On-Line Smart DO Analyzer | |
| Zhang et al. | A low-cost mobile platform for whole blood glucose monitoring using colorimetric method | |
| CN106442515B (en) | A kind of visual quantitative detecting method of simple silver ion | |
| US4314029A (en) | Apparatus for automatically measuring changes in amount of gas | |
| JP5076142B2 (en) | Fluorometer | |
| Phillips et al. | Advances in materials that enable quantitative point-of-care assays | |
| CN205643151U (en) | Portable hydrogen peroxide detector | |
| CN101127792B (en) | Mobile communication terminal with temperature compensation function in biological information measurement | |
| CN206906239U (en) | Water quality detection probe and water quality testing meter | |
| Yu et al. | An open-source handheld spectrometer for colorimetric and fluorescence analyses | |
| CN105911005A (en) | Hydrogen peroxide photometric method adopting DhHP-6 mimic enzyme | |
| CN201788153U (en) | Pesticide residue detector integrated with micro electronic balance | |
| CN203881687U (en) | Hand-held rapid pesticide residue determinator | |
| KR20110004676U (en) | The Residual Pesticides measurement system using bio electrode | |
| CN114878668B (en) | Full-automatic dry three-electrode electrochemiluminescence analyzer and application thereof in electrochemiluminescence detection | |
| CN201047840Y (en) | Twig hemo-ethanol detecting instrument | |
| Smith et al. | An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification | |
| CN107884402B (en) | Hydrogen peroxide integrated rapid detection tube and method for rapidly detecting hydrogen peroxide | |
| JP5811632B2 (en) | Carbon dioxide concentration meter | |
| Reinthaler et al. | Fully automated spectrophotometric approach to determine oxygen concentrations in seawater via continuous‐flow analysis | |
| Cheng et al. | A low-cost compact blood enzyme analyzer based on optical sensing for point-of-care liver function testing | |
| CN116519609B (en) | Multi-parameter sensor and intelligent detection device for water quality detection | |
| Yang et al. | A cost-efficient and portable sulfide device with in situ integrating gas-permeable porous tube isolation and long path absorbance detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161012 Termination date: 20200523 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |