CN205620305U - Biochip detection device that laser induction and CCD gathered - Google Patents
Biochip detection device that laser induction and CCD gathered Download PDFInfo
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- CN205620305U CN205620305U CN201620354111.7U CN201620354111U CN205620305U CN 205620305 U CN205620305 U CN 205620305U CN 201620354111 U CN201620354111 U CN 201620354111U CN 205620305 U CN205620305 U CN 205620305U
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Abstract
The utility model relates to a biochip detection device that laser induction and CCD gathered provides a quilt detection biochip, include: set up in directly over the biochip and the CCD camera, the symmetry that are used for image acquisition set up in CCD camera both sides first shake mirror unit and second shake the mirror unit, with first shake the mirror unit and shake mirror unit cooperation and be used for providing a laser source and a computer that arouses biochip to produce the fluorescence signal of second, the computer shakes the mirror unit with CCD camera, first shake mirror unit and second respectively and links to each other. The utility model provides a biochip detection device that laser induction and CCD gathered has avoided the complicated light path of laser copolymerization jiao method and the illumination problem of high -pressure xenon lamp, provides higher detection speed and sensitivity.
Description
Technical field
This utility model relates to biochip test, the bio-chip test device that a kind of induced with laser gathers with CCD.
Background technology
Patent (application number: CN200410009044.7) proposes a kind of biochip test method with the detection of light hard real time and detecting system so that biological chips detection system can be detected process in time and make detection data accurate when there is light source intensity change conditions during detection.Patent (application number: CN03112771.1) proposes a kind of low-density biochip detecting system, in conjunction with exciting light system, fluorescence signal collection system and signal detection system, Chief technology is, with fibre-optic bundle, to photomultiplier transit tube-surface and the fluorescence signal collection produced on chip is changed into the signal of telecommunication, draws a kind of low cost, the method being applicable to detect low-density biochip fluorescence signal.Patent (application number: CN201110398112.3) proposes a kind of bio-chip test device and biochip test method, by judge identification information to determine whether biochip can detect, and through controller automatically adjust detection module setting reduce mistake.
Above-mentioned common biochip test method substantially has two kinds, one of which is that the mode using laser confocal scanning and photomultiplier tube collection carries out biochip test, but this kind of method is due to the laser focusing after expanding to the speckle of the most several micron levels, and rely on two-dimensional scanner that biochip is scanned so can cause a worrying problem of comparison is exactly that the speed detected is slow.Another kind of method is then to use based on cooling type CCD and the detection mode of high pressure xenon gas lamp, but this kind of detection method has one to a problem is also that the lighting source life-span of high pressure xenon gas lamp that it is used is comparatively short than the scan sensitivity that more serious shortcoming is exactly it than relatively low.
Summary of the invention
The purpose of this utility model is to provide the bio-chip test device that a kind of induced with laser gathers with CCD, to overcome defect present in prior art.
For achieving the above object, the technical solution of the utility model is: the bio-chip test device that a kind of induced with laser gathers with CCD, there is provided and one be detected biochip, including: it is arranged at directly over described biochip and for the CCD camera of fluorescence signal image acquisition, the first galvanometer unit being symmetricly set in described CCD camera both sides and the second galvanometer unit and described first galvanometer unit and described second galvanometer unit matching and for providing lasing light emitter and the computer exciting described biochip to produce fluorescence signal；Described computer is connected with described CCD camera, described first galvanometer unit and described second galvanometer unit respectively.
In this utility model one embodiment, described CCD camera includes light receiving microscopy head and the cooling type CCD camera set gradually from the bottom to top；Described light receiving microscopy head includes micro-lens and arrowband coated filter.
In this utility model one embodiment, described cooling type CCD camera is ICX694AL CCD.
In this utility model one embodiment, described first galvanometer unit and described second galvanometer unit all include a high-speed vibrating mirror；Described high-speed vibrating mirror is connected with high-speed vibrating mirror drive circuit, amplification subtraction circuit, control circuit and described computer successively.
In this utility model one embodiment, the high-speed vibrating mirror in described first galvanometer unit and described second galvanometer unit is in 90 degree of orthogonal settings.
In this utility model one embodiment, described lasing light emitter includes the directional light laser instrument of a fixed frequency, and provides rectangular light spot through a square diaphragm for described first galvanometer unit and described second galvanometer unit.
Compared to prior art, this utility model has the advantages that the bio-chip test device that the induced with laser that utility model is proposed gathers with CCD, both complex optical path and the lighting problem of high pressure xenon gas lamp of laser confocal methods had been avoided, it also is able to ensure higher detection speed and sensitivity, so that the sensitivity of detection is better than 1flour/um2, the detection time of single channel scanning 22mm*22mm, compared to simple in construction for the laser confocal scanning detection mode of mainstream applications, scanning speed was fast less than 55 seconds.This product can be widely applied to the aspects such as medical diagnosis on disease, drug screening, preventive medicine, it is possible to solves the effect that some traditional detection modes are not accomplished.
Accompanying drawing explanation
Fig. 1 is the structural representation of the bio-chip test device that this utility model induced with laser gathers with CCD.
Fig. 2 is the circuit theory diagrams of the bio-chip test device that this utility model induced with laser gathers with CCD.
Fig. 3 is the driving control circuit schematic diagram of this utility model two-dimensional scanning mirrors.
Fig. 4 is this utility model laser index path through vibration mirror reflected to chip surface.
Detailed description of the invention
Below in conjunction with the accompanying drawings, the technical solution of the utility model is specifically described.
This utility model provides the bio-chip test device that a kind of induced with laser gathers with CCD, as shown in Figure 1 and Figure 2, there is provided and one be detected biochip, including: be arranged at directly over biochip and for fluorescence signal image acquisition CCD camera, be symmetricly set in CCD camera both sides and the first galvanometer unit of lower section and the second galvanometer unit and the first galvanometer unit and the second galvanometer unit matching and for providing lasing light emitter and the computer exciting biochip to produce fluorescence signal；Computer is connected with CCD camera, the first galvanometer unit and the second galvanometer unit respectively.
Further, in the present embodiment, CCD camera includes light receiving microscopy head and the cooling type CCD camera set gradually from the bottom to top；Light receiving microscopy head includes micro-lens and arrowband coated filter.
Further, in the present embodiment, cooling type CCD camera is ICX694AL CCD.
Further, in the present embodiment, as it is shown on figure 3, the first galvanometer unit and the second galvanometer unit all include a high-speed vibrating mirror；High-speed vibrating mirror successively with, high-speed vibrating mirror drive circuit, amplify subtraction circuit, control circuit and computer and be connected.
Further, in the present embodiment, the high-speed vibrating mirror correspondence in the first galvanometer unit and the second galvanometer unit becomes 90 degree of orthogonal settings.
Further, in the present embodiment, lasing light emitter includes the directional light laser instrument of a fixed frequency, and provides rectangular light spot through a square diaphragm for the first galvanometer unit and the second galvanometer unit.
In order to allow those skilled in the art further appreciate that the bio-chip test device that the induced with laser that this utility model is proposed gathers with CCD; below in conjunction with existing software or control method, this device is illustrated; existing software involved in this declarative procedure is not the object that this utility model is protected, and this utility model only protects structure and the annexation thereof of this device.
After placing biochip to be detected, by configuring the two-dimensional scan model pre-set in a computer, make the LASER Light Source with constant power launch laser and carry out two-dimensional scan by X-direction and the Y-direction at biochip that control of two-dimensional scanning mirrors, as shown in Fig. 1 and Fig. 4, 1 is the high-speed vibrating mirror in the first galvanometer unit, 2 is the high-speed vibrating mirror in the second galvanometer unit, the central shaft distance of two high-speed vibrating mirror is e, laser Oblique 45 Degree under the work of two-dimensional scanning mirrors is irradiated to biochip plane, and excite CY3 or CY5 fluorescent dye to produce fluorescence signal by the imaging of cooling type CCD camera, signal to be acquired.After the part scanning whole probe plane, stop scanning a period of time and carry out image acquisition, transfer data to immediately after collecting image computer stores, it is further continued for carrying out two-dimensional scan after storage so to circulate until by complete for whole biochip probe flat scanning, carrying out the denoising of image in computer system and the image integration of piecemeal becomes the biological chip fluorescent picture that a width is complete.The most both complex optical path and the lighting problem of high pressure xenon gas lamp of laser confocal methods had been avoided, it is also possible to ensure higher detection speed and sensitivity.
It is above preferred embodiment of the present utility model, all changes made according to technical solutions of the utility model, when produced function is without departing from the scope of technical solutions of the utility model, belong to protection domain of the present utility model.
Claims (6)
1. the bio-chip test device that an induced with laser gathers with CCD, one is provided to be detected biochip, it is characterized in that, including: it is arranged at directly over described biochip and for the CCD camera of fluorescence signal image acquisition, the first galvanometer unit being symmetricly set in described CCD camera both sides and the second galvanometer unit and described first galvanometer unit and described second galvanometer unit matching and for providing lasing light emitter and the computer exciting described biochip to produce fluorescence signal；Described computer is connected with described CCD camera, described first galvanometer unit and described second galvanometer unit respectively.
The bio-chip test device that a kind of induced with laser the most according to claim 1 gathers with CCD, it is characterised in that described CCD camera includes light receiving microscopy head and the cooling type CCD camera set gradually from the bottom to top；Described light receiving microscopy head includes micro-lens and arrowband coated filter.
3. the bio-chip test device gathered with CCD according to a kind of induced with laser described in claim 2, it is characterised in that described cooling type CCD camera is ICX694AL
CCD。
The bio-chip test device that a kind of induced with laser the most according to claim 1 gathers with CCD, it is characterised in that described first galvanometer unit and described second galvanometer unit all include a high-speed vibrating mirror；Described high-speed vibrating mirror is connected with high-speed vibrating mirror drive circuit, amplification subtraction circuit, control circuit and described computer successively.
The bio-chip test device that a kind of induced with laser the most according to claim 4 gathers with CCD, it is characterised in that the high-speed vibrating mirror in described first galvanometer unit and described second galvanometer unit is in 90 degree of orthogonal settings.
The bio-chip test device that a kind of induced with laser the most according to claim 1 gathers with CCD, it is characterized in that, described lasing light emitter includes the directional light laser instrument of a fixed frequency, and provides rectangular light spot through a square diaphragm for described first galvanometer unit and described second galvanometer unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620354111.7U CN205620305U (en) | 2016-04-26 | 2016-04-26 | Biochip detection device that laser induction and CCD gathered |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620354111.7U CN205620305U (en) | 2016-04-26 | 2016-04-26 | Biochip detection device that laser induction and CCD gathered |
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| Publication Number | Publication Date |
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| CN205620305U true CN205620305U (en) | 2016-10-05 |
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| CN201620354111.7U Active CN205620305U (en) | 2016-04-26 | 2016-04-26 | Biochip detection device that laser induction and CCD gathered |
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2016
- 2016-04-26 CN CN201620354111.7U patent/CN205620305U/en active Active
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