CN205506748U - Three -dimensional graphite alkene lactic acid sensor - Google Patents
Three -dimensional graphite alkene lactic acid sensor Download PDFInfo
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- CN205506748U CN205506748U CN201620050298.1U CN201620050298U CN205506748U CN 205506748 U CN205506748 U CN 205506748U CN 201620050298 U CN201620050298 U CN 201620050298U CN 205506748 U CN205506748 U CN 205506748U
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Abstract
The utility model provides a three -dimensional graphite alkene lactic acid sensor, this sensor are by flexible substrate, working electrode, constitute electrode, reference electrode and the insulating thick liquids that cover the electrode surface, working electrode's functional layer comprises three -dimensional graphite alkene, lactic acid oxidizing ferment, chitosan and glutaraldehyde supreme down in proper order, and wherein, the two -dimentional graphite alkene assembly of three -dimensional graphite alkene for having the 3D structure specifically is one kind by the graphite alkene nanometer wall structure of graphite alkene nanometer piece vertical distribution on substrate material. The utility model provides a three -dimensional graphite alkene lactic acid sensor has overcome the not enough of current conventional lactic acid sensor, can make graphite alkene lactic acid sensor with economic environmental protection, simple high efficiency and the controllable mode of quality, and abundant three -dimensional electrically conductive network structure and the huge advantages such as specific surface of graphite alkene nanometer wall has promoted sensitivity and the real -time that detects lactic acid effectively, owing to adopted flexible substrate, this sensor can be used to the healthy field of medical care and public health simultaneously.
Description
Technical field
This utility model relates to a kind of three-dimensional grapheme lactic acid sensor, belongs to biochemical sensor technology neck
Territory, is specifically related to a kind of wearable three-dimensional grapheme lactic acid sensor.
Background technology
2013, from the Jia Wenzhao et al. of California, USA Santiago university at Analytical
Chemical magazine ran one is lactic acid sensor (W.Jia, the A.J. of lactic acid monitoring in human sweat
Bandodkar,G.Valdes-Ramirez,J.R.Windmil-ler,Z.Yang,J.Ramirez,G.Chan,J.
Wang, Anal.Chem.2013,85,6,553 6560.), this lactic acid sensor is the noinvasive of a kind of miniaturization
Wearable sensor, can be used for the real-time monitoring of lactic acid, but this sensor is with multilayer carbon nanotube as base
Material, its preparation technology is complex, and electrical conductivity and biocompatibility poor.
Graphene is by monolayer two dimension (2D) carbonaceous material of the tightly packed one-tenth of carbon atom.Fabulous owing to having
The performances such as electricity, optics, machinery, Graphene is the most concerned.Three-dimensional (3D) Graphene typically refers to have 3D
Two dimension (2D) the Graphene assembly of structure, is the new type functional material of Graphene chemical field in recent years.
2D graphene film is integrated into have 3D structure assembly can with the electricity of Effective Regulation Graphene, optics,
Machinery, chemistry and catalysis characteristics, therefore 3D grapheme material not only has the physicochemical property that Graphene is intrinsic,
Its three-dimensional porous micrometer structure also makes it have concurrently, and specific surface area is big, mechanical strength is high, electronic conduction ability is superior
And the mass transfer good characteristic such as quickly.The character of these uniquenesses makes 3D ink alkene and composite thereof lead in material science
Territory receives much concern.
Application publication number is the lactic biological that CN105116028A patent document discloses a kind of graphene modified
Sensor, this lactic biological sensor is the lactic acid sensor of a kind of miniaturization, can measure the breast in human serum
Acid content, but this material still belongs to the Wearable lactic acid sensor that carbon granule/carbon fiber is base material of two dimension,
Its electrical conductivity, biocompatibility still have limitation, and the tensile property of material own is the strongest.This patent simultaneously
Using oxidation or carboxylated Graphene, material itself, due to its fault of construction, causes its electric transmission speed
Spend the highest, affect the efficiency of sensor.
Utility model content
For the deficiency in background technology and Improvement requirement, the purpose of this utility model is to provide a kind of three-dimensional stone
Ink alkene lactic acid sensor, based on a kind of special graphene nano wall construction material as the load of Lactate Oxidase
Skeleton.A kind of three-dimensional grapheme lactic acid sensor involved by this utility model, by flexible substrate, working electrode,
To electrode, reference electrode and the insulation paste composition covering electrode surface；
Described flexible substrate is PET or polyimides is the flexible polymer of base material；
Described working electrode is made up of carbon paste wire, carbon paste electrode and working electrode functional layer；Described working electrode
Functional layer is collectively constituted by three-dimensional grapheme and enzyme functional layer；Enzyme functional layer is followed successively by Lactate Oxidase from the bottom to top
Solution, chitosan solution, glutaraldehyde solution, wherein, Lactate Oxidase solution concentration is 100-800IU/ml,
Chitosan solution concentration is 0.3-3.5%, and glutaraldehyde solution concentration is 0.25-2.0%；
Described is to be collectively constituted by carbon paste wire and carbon paste electrode electrode；
Described reference electrode is to be collectively constituted by silver wire and silver/silver chloride electrode.
Further, the three-dimensional grapheme in described working electrode functional layer is the two-dimensional graphene with 3D structure
Assembly, a kind of graphene nano wall knot by graphene nanometer sheet vertical distribution on base material
Structure.
The three-dimensional grapheme lactic acid sensor that this utility model provides overcomes existing conventional lactic acid sensor not
The advantages such as foot, three-dimensional conductive network structure that graphene nano wall is abundant and huge specific surface area promote effectively
Susceptiveness and real-time to lactate detection, simultaneously because have employed flexible substrate, tensility is strong, suitably
Dressing, this sensor can be used on the electronic skin that Noninvasive is wearable, human body lactic acid can be carried out in real time,
Accurate detection, is particularly suited for the application in the field such as medical monitoring, health control.
Accompanying drawing explanation
Fig. 1 is the structural representation of the three-dimensional grapheme lactic acid sensor involved by this utility model；
Fig. 2 is the profile of the A-A` of Fig. 1；
Fig. 3 is the profile of the B-B` of Fig. 1；
In Fig. 1 to Fig. 3, the title corresponding to each labelling is respectively: 1 is flexible substrate；2 and 3 is carbon paste
Wire；4 is silver wire；5 is insulating barrier；6 and 10 is that carbon paste is to electrode；7 and 12 is carbon paste working electrode；
9 is working electrode functional layer；8 and 11 is silver/silver chloride reference electrode；13 is three-dimensional grapheme；14 is penta
Dialdehyde；15 is chitosan；16 is Lactate Oxidase.
Fig. 4 is the structural representation of three-dimensional grapheme 13 in Fig. 1 to Fig. 3.
Fig. 5 is the scanning electron microscope (SEM) photograph of three-dimensional grapheme 13.
Fig. 6 is the Raman spectrum of three-dimensional grapheme 13.
Detailed description of the invention
With embodiment, this utility model is illustrated below in conjunction with the accompanying drawings.
Embodiment 1
Present embodiments provide a kind of three-dimensional grapheme lactic acid sensor, this lactic acid sensor structure such as Fig. 1-Fig. 3
Shown in, by flexible PET substrate 1, working electrode, to electrode, reference electrode with cover the exhausted of electrode surface
Edge slurry 5 is constituted.Working electrode is by carbon paste wire 3, carbon paste electrode 7 and carbon paste electrode 12 and work electricity
Pole functional layer 9 forms；Working electrode functional layer 9 is collectively constituted by three-dimensional grapheme 13 and enzyme functional layer；Enzyme
Functional layer is followed successively by Lactate Oxidase solution 16 from the bottom to top, chitosan solution 15, glutaraldehyde solution 14 coat
Forming, wherein, Lactate Oxidase concentration is 100IU/ml, and chitosan solution concentration used is 0.3%, institute
Glutaraldehyde solution concentration be 0.25%.It is by carbon paste wire 2, carbon paste electrode 6 and carbon paste electrode to electrode
10 collectively constitute.Reference electrode is to be collectively constituted by silver wire 4, silver/silver chloride electrode 8 and 11.
Wherein, the structural representation of three-dimensional grapheme 13 as shown in Figure 4, its scanning electron microscope (SEM) photograph and Raman spectrum
As shown in Figure 5 and Figure 6.
Embodiment 2
Present embodiments provide a kind of three-dimensional grapheme lactic acid sensor, this lactic acid sensor structure such as Fig. 1-Fig. 3
Shown in, by flexible substrate 1 be polyimide material, working electrode, to electrode, reference electrode with cover electric
The insulation paste 5 on surface, pole is constituted.Working electrode is by carbon paste wire 3, carbon paste electrode 7 and carbon paste electrode 12
And working electrode functional layer 9 forms；Working electrode functional layer 9 is total to by three-dimensional grapheme 13 and enzyme functional layer
With composition；Enzyme functional layer is followed successively by Lactate Oxidase solution 16, chitosan solution 15, glutaraldehyde from the bottom to top
Solution 14 coating forms, and wherein, Lactate Oxidase concentration is 800IU/ml, chitosan solution concentration used
Being 3.5%, glutaraldehyde solution concentration used is 2.0%.It is by carbon paste wire 2, carbon paste electrode 6 to electrode
Collectively constitute with carbon paste electrode 10.Reference electrode is by silver wire 4, the common group of silver/silver chloride electrode 8 and 11
Become.
Claims (2)
1. a three-dimensional grapheme lactic acid sensor, by flexible substrate, working electrode, to electrode, reference electrode and cover electrode surface insulation paste constitute, it is characterised in that:
Described flexible substrate is PET or polyimides is the flexible polymer of base material；
Described working electrode is made up of carbon paste wire, carbon paste electrode and working electrode functional layer；
Described working electrode functional layer is collectively constituted by three-dimensional grapheme and enzyme functional layer, and enzyme functional layer is followed successively by Lactate Oxidase layer, chitosan layer, glutaraldehyde layer from the bottom to top；
Described is to be collectively constituted by carbon paste wire and carbon paste electrode electrode；
Described reference electrode is to be collectively constituted by silver wire and silver/silver chloride electrode.
A kind of three-dimensional grapheme lactic acid sensor the most according to claim 1, it is characterised in that the three-dimensional grapheme in described working electrode functional layer is the two-dimensional graphene assembly with 3D structure.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106525915A (en) * | 2016-09-30 | 2017-03-22 | 山东师范大学 | Support vector machine based foamed graphene sensor sample detection method and system |
| CN107271517A (en) * | 2017-07-03 | 2017-10-20 | 中国人民解放军第三军医大学 | A kind of sweat lactic acid device for fast detecting |
| CN107576698A (en) * | 2017-10-30 | 2018-01-12 | 中国农业科学院农业信息研究所 | The detection means of tanning matter and its application in a kind of fruits and vegetables |
| CN111007127A (en) * | 2019-12-24 | 2020-04-14 | 湖南海源医疗科技股份有限公司 | Application of ordered vertical porous graphene in preparation of blood glucose test paper |
| CN113311033A (en) * | 2021-04-29 | 2021-08-27 | 苏州中星医疗技术有限公司 | Lactate biosensor |
| CN113390937A (en) * | 2021-06-11 | 2021-09-14 | 上海大学 | Wearable flexible printed electrode |
-
2016
- 2016-01-19 CN CN201620050298.1U patent/CN205506748U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106525915A (en) * | 2016-09-30 | 2017-03-22 | 山东师范大学 | Support vector machine based foamed graphene sensor sample detection method and system |
| CN107271517A (en) * | 2017-07-03 | 2017-10-20 | 中国人民解放军第三军医大学 | A kind of sweat lactic acid device for fast detecting |
| CN107271517B (en) * | 2017-07-03 | 2019-06-25 | 中国人民解放军第三军医大学 | A kind of sweat lactic acid device for fast detecting |
| CN107576698A (en) * | 2017-10-30 | 2018-01-12 | 中国农业科学院农业信息研究所 | The detection means of tanning matter and its application in a kind of fruits and vegetables |
| CN107576698B (en) * | 2017-10-30 | 2023-06-16 | 中国农业科学院农业信息研究所 | Detection device for tannin substances in fruits and vegetables and application thereof |
| CN111007127A (en) * | 2019-12-24 | 2020-04-14 | 湖南海源医疗科技股份有限公司 | Application of ordered vertical porous graphene in preparation of blood glucose test paper |
| CN113311033A (en) * | 2021-04-29 | 2021-08-27 | 苏州中星医疗技术有限公司 | Lactate biosensor |
| CN113390937A (en) * | 2021-06-11 | 2021-09-14 | 上海大学 | Wearable flexible printed electrode |
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