CN205506113U - Intelligent monitoring system based on organizational project hydraulic pressure bioreactor - Google Patents
Intelligent monitoring system based on organizational project hydraulic pressure bioreactor Download PDFInfo
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- CN205506113U CN205506113U CN201620271791.6U CN201620271791U CN205506113U CN 205506113 U CN205506113 U CN 205506113U CN 201620271791 U CN201620271791 U CN 201620271791U CN 205506113 U CN205506113 U CN 205506113U
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Abstract
The utility model discloses an intelligent monitoring system based on organizational project hydraulic pressure bioreactor, this system includes current sensor module, constant -potential circuit, signal conditioning module, data acquisition and processing unit and host computer, current sensor module setting is in bioreactor in the culture solution, current sensor module, signal conditioning module, data acquisition and processing unit and host computer are consecutive, data acquisition passes through constant -potential circuit with processing unit and links to each other with the current sensor module. Adopt three electrode type needle -like sensors to realize reactor environmental parameter's such as oxygen content in the culture solution, glucose content, pH value size and humiture, pressure accuracy, spot test and on -line monitoring, constant -potential circuit makes working electrode keep invariable for reference electrode's current potential, guarantees that electrochemical reaction stably goes on to make measured signal through the working electrode firm output, in carrying the host computer with stable measured signal, bioreactor's the real -time on -line monitoring of parameter and the accurate prediction of microenvironment have been realized.
Description
Technical field
This utility model belongs to intelligent instrument and the biomedical crossing domain combined, and relates to a kind of intellectual faculties based on organizational project hydraulic pressure bioreactor.
Background technology
Organizational project (Tissue Engineering) is one of field of greatest concern in the most biomedical international research, belongs to the multi-crossed disciplines fields such as human medical, cytobiology, molecular biology, materialogy, design engineering.Concept based on organizational project, scientist the most tentatively realizes utilizing the most whole organs of element designs and manufacturer soma such as seed cell, biomaterial and somatomedin.Having millions of people to suffer from various tissue, the forfeiture of organ and functional disorder every year, up-to-date tissue engineering product damages for repairing and improves defect to need various biomaterial to include, demand will the most quickly increase.
Organizational project is on the basis of multi-crossed disciplines, by cytobiology and the mutual fusion of biomaterial, builds in vitro or in vivo and has normal configuration and the particular organization of function or organ, be used for repairing and improving human body damaged tissues or organ.The implantation body of human body defect is repaired in order to make the organizational structure of acquisition become, various countries develop Various Tissues engineered biological reactor, purpose is to provide for tissue to be similar to internal microenvironment, promote intercellular various effect of mass transmitting, and control oxygen content, pH value, glucose and humiture, pressure and other parameters.Tissue engineering reactor is mainly used for the In vitro culture of the tissues such as blood vessel, tendon, skin, cartilage, liver at present, can be divided into mechanical agitation type bioreactor, airlift bioreactor, film biological reactor, microcarrier reactor, hollow fiber reactor, filling type bioreactor, rotating wall vessel bioreactor etc..
The reaction process monitoring of culture fluid in cultured tissue cell processes is rested on always to the stage of dependence expertise with discrimination, this micro-judgment method cannot realize detection qualitative, accurate and real-time data analysis, culture fluid is caused to waste, and artificially contact culture fluid easily causes histiocyte pollution, it is impossible to the problem fundamentally solving bioreactor intelligent monitoring and asepticization cultivation.
Summary of the invention
In order to overcome at present reaction process monitoring and discrimination present in biomedical upper histiocyte incubation, improving the intelligent monitoring degree of existing bioreactor system, the purpose of this utility model is to provide a kind of intellectual faculties based on organizational project hydraulic pressure bioreactor.
A kind of intellectual faculties based on organizational project hydraulic pressure bioreactor, including current sensor module, potentiostatic circuit, Signal-regulated kinase, data acquisition and procession unit and host computer；
Described current sensor module is arranged in bioreactor in culture fluid；
Described current sensor module, Signal-regulated kinase, data acquisition and procession unit and host computer are sequentially connected；
Described data acquisition and procession unit is connected with current sensor module by potentiostatic circuit.
Described current sensor module is three electrode needle tubular sensors, a diameter of 500 μm, including triple electrode, is followed successively by working electrode from the inside to the outside, to electrode and reference electrode；
Described working electrode uses the Pt silk of 5 μm to make；
Described to electrode employing sintered glass making, a diameter of 125 μm；
Described reference electrode uses rustless steel to make；
It is solidified with enzyme on described working electrode.
Enzyme is solidificated on working electrode Pt silk, and during working sensor, enzyme contacts with culture fluid in bioreactor, electrochemical reaction occurs around working electrode under certain external signal stimulates, produces weak current.
Described reference electrode and to being provided with epoxy resin layer between electrode.
Epoxy resin layer is used for encapsulating fixing.
Voltage compensation module it is additionally provided with on the voltage input end of described potentiostatic circuit.
Also include the wireless transmission unit being arranged between data acquisition and procession unit and host computer.
Described Signal-regulated kinase includes DAC, operational amplifier, I/V modular converter and automatic gear selecting circuit.
Described data acquisition and procession unit is the C8051F064 chip of Silicon Lab company.
Described automatic gear selecting circuit is multiplexer, and model is the MAX4617 of Maxim company.
The described OP777/AD8608/AD8627 that operational amplifier model is ADI company.
Described wireless transmission unit is NI WSN module.
The described nanoDAC series A D5060 that DAC conversion chip model is ADI company.
Described host computer is that LabVIEW virtual instrument host computer manages platform.
The wireless sensing module using NI company is measured node and NI WSN-9791 gateway by NI WSN-3231 and is formed, and can realize the long-range monitoring of sensor acquisition data, rapidly and efficiently and also reliable communications.
Potentiostatic circuit module uses the low-power consumption precision operational-amplifier composition of three ADI companies, and three amplifiers are respectively as signal input, voltage follower and the effect of current/voltage conversion.External drive signal, as the interface circuit of three electrode sensors, can be applied on reference electrode by this circuit exactly, it is achieved working electrode keeps constant relative to the current potential on reference electrode, and can be automatically adjusted.
Three electrode needle-like sensors are placed in bioreactor in culture fluid, and driving voltage signal is exported by MCU by DA conversion chip, and signal acts on the reference electrode of three electrode needle-like sensors through potentiostatic circuit, in becoming to making bioreactor, electrochemical reaction occur.Potentiostatic circuit makes working electrode keep constant relative to the current potential of reference electrode, it is ensured that electrochemical reaction is stably carried out, and makes measured signal stably export through working electrode.Weak current is converted into voltage signal through I/V conversion and automatic gear selecting amplifying circuit by measured signal, it is input to the treatable scope of ADC that MCU carries again, then the signal after conditioning is sent into MCU be AD converted and carry out data process, data after process can store through external memory storage, it is also possible to through radio sensing network, data is uploaded to host computer and carries out subsequent treatment and analysis.
Beneficial effect
This utility model provides a kind of intellectual faculties based on organizational project hydraulic pressure bioreactor, and this system includes current sensor module, potentiostatic circuit, Signal-regulated kinase, data acquisition and procession unit and host computer；Described current sensor module is arranged in bioreactor in culture fluid；Described current sensor module, Signal-regulated kinase, data acquisition and procession unit and host computer are sequentially connected；Described data acquisition and procession unit is connected with current sensor module by potentiostatic circuit.Three electric pole type needle-like sensors are used to realize mensuration accurate, quick and the on-line monitoring of oxygen content in culture fluid, glucose content, pH value size and the reactor ambient parameter such as humiture, pressure, potentiostatic circuit makes working electrode keep constant relative to the current potential of reference electrode, ensure that electrochemical reaction is stably carried out, and make measured signal stably export through working electrode；Stable measured signal is transported in host computer, it is achieved that the parameter real time on-line monitoring of bioreactor and the Accurate Prediction of microenvironment.
Accompanying drawing explanation
Fig. 1 is detecting system theory diagram of the present utility model；
Fig. 2 is three electrode needle tubular sensor generalized sections in this utility model；
Fig. 3 is the potentiostatic circuit schematic diagram used in this utility model；
Label declaration: 1-sintered glass, 2-5 μm platinum filament, 3-enzyme, 4-epoxy resin, 5-rustless steel.
Detailed description of the invention
Below in conjunction with drawings and Examples, this utility model is described further.
As it is shown in figure 1, a kind of intellectual faculties based on organizational project hydraulic pressure bioreactor, including current sensor module, potentiostatic circuit, Signal-regulated kinase, data acquisition and procession unit and host computer；
Described current sensor module is arranged in bioreactor in culture fluid；
Described current sensor module, Signal-regulated kinase, data acquisition and procession unit and host computer are sequentially connected；
Described data acquisition and procession unit is connected with current sensor module by potentiostatic circuit.
As in figure 2 it is shown, described current sensor module is three electrode needle tubular sensors, a diameter of 500 μm, including triple electrode, it is followed successively by working electrode from the inside to the outside, to electrode and reference electrode；
Described working electrode uses the Pt silk 2 of 5 μm to make；
Described to electrode use sintered glass 1 make, a diameter of 125 μm；
Described reference electrode uses rustless steel 5 to make；
Enzyme 3 it is solidified with on described working electrode.
Enzyme is solidificated on working electrode Pt silk, and during working sensor, enzyme contacts with culture fluid in bioreactor, electrochemical reaction occurs around working electrode under certain external signal stimulates, produces weak current.
Described reference electrode and to being provided with epoxy resin 4 layers between electrode.
Epoxy resin layer is used for encapsulating fixing.
As it is shown on figure 3, be additionally provided with voltage compensation module on the voltage input end of described potentiostatic circuit.
It is easily subject to interference during pumping signal V_in1 input potentiostatic circuit, current potential between two electrodes is changed, therefore to exactly by the most constant for the current potential between two electrodes for setting value, introduce another road fine-tuning signal V_in2 while input signal and carry out voltage compensation, use accurate reference voltage and adjustable resistance that input signal is accurately regulated, it is possible to keep the current potential between two electrodes the most constant.
Also include the wireless transmission unit being arranged between data acquisition and procession unit and host computer.
Described Signal-regulated kinase includes DAC, operational amplifier, I/V modular converter and automatic gear selecting circuit.
Described data acquisition and procession unit is the C8051F064 chip of Silicon Lab company.
Pumping signal is sent into DA chip AD5060 by SPI interface and is changed by MCU chip C8051F064, analog voltage is exported through D/A, act on homemade three electrode sensors by potentiostatic circuit again so that electrochemical reaction occurs on working electrode, produces weak current to be detected.On the one hand the effect of potentiostatic circuit is to ensure that constant relative to the current potential on reference electrode or the regular change of working electrode, makes electrochemical reaction stable；On the one hand it is that by being automatically adjusted the working electrode current potential relative to reference electrode when change occurs in circuit parameter, keeps the function that working electrode potential is constant.
Described automatic gear selecting circuit is multiplexer, and model is the MAX4617 of Maxim company, it is ensured that the output voltage of this module facilitates follow-up conditioning and A/D sampling.
The described OP777/AD8608/AD8627 that operational amplifier model is ADI company.
Described wireless transmission unit is NI WSN module.
The described nanoDAC series A D5060 that DAC conversion chip model is ADI company.
Described host computer is that LabVIEW virtual instrument host computer manages platform.
Intellectual faculties based on organizational project hydraulic pressure bioreactor use Virtual instrument LabVIEW management platform data are carried out subsequent treatment and management, the host computer data to receiving carry out valid data intercepting, and carry out correlation computations and be derived by current value to be measured, current value is carried out Treatment Analysis, result and statistical analysis charts are shown.
The wireless sensing module using NI company is measured node and NI WSN-9791 gateway by NI WSN-3231 and is formed, and can realize the long-range monitoring of sensor acquisition data, rapidly and efficiently and also reliable communications.
Potentiostatic circuit module uses the low-power consumption precision operational-amplifier composition of three ADI companies, and three amplifiers are respectively as signal input, voltage follower and the effect of current/voltage conversion.External drive signal, as the interface circuit of three electrode sensors, can be applied on reference electrode by this circuit exactly, it is achieved working electrode keeps constant relative to the current potential on reference electrode, and can be automatically adjusted.
Three electrode needle-like sensors are placed in bioreactor in culture fluid, and driving voltage signal is exported by MCU by DA conversion chip, and signal acts on the reference electrode of three electrode needle-like sensors through potentiostatic circuit, in becoming to making bioreactor, electrochemical reaction occur.Potentiostatic circuit makes working electrode keep constant relative to the current potential of reference electrode, it is ensured that electrochemical reaction is stably carried out, and makes measured signal stably export through working electrode.Weak current is converted into voltage signal through I/V conversion and automatic gear selecting amplifying circuit by measured signal, it is input to the treatable scope of ADC that MCU carries again, then the signal after conditioning is sent into MCU be AD converted and carry out data process, data after process can store through external memory storage, it is also possible to through radio sensing network, data is uploaded to host computer and carries out subsequent treatment and analysis.
Claims (10)
1. an intellectual faculties based on organizational project hydraulic pressure bioreactor, it is characterised in that include current sensor module, potentiostatic circuit, Signal-regulated kinase, data acquisition and procession unit and host computer；
Described current sensor module is arranged in bioreactor in culture fluid；
Described current sensor module, Signal-regulated kinase, data acquisition and procession unit and host computer are sequentially connected；
Described data acquisition and procession unit is connected with current sensor module by potentiostatic circuit.
System the most according to claim 1, it is characterised in that described current sensor module is three electrode needle tubular sensors, a diameter of 500 μm, including triple electrode, is followed successively by working electrode, from the inside to the outside to electrode and reference electrode；
Described working electrode uses the Pt silk of 5 μm to make；
Described to electrode employing sintered glass making, a diameter of 125 μm；
Described reference electrode uses rustless steel to make；
It is solidified with enzyme on described working electrode.
System the most according to claim 2, it is characterised in that described reference electrode and to being provided with epoxy resin layer between electrode,
Epoxy resin layer is used for encapsulating fixing.
4. according to the system described in any one of claim 1-3, it is characterised in that be additionally provided with voltage compensation module on the voltage input end of described potentiostatic circuit.
System the most according to claim 4, it is characterised in that also include the wireless transmission unit being arranged between data acquisition and procession unit and host computer.
System the most according to claim 5, it is characterised in that described Signal-regulated kinase includes DAC, operational amplifier, I/V modular converter and automatic gear selecting circuit.
System the most according to claim 6, it is characterised in that described data acquisition and procession unit is the C8051F064 chip of Silicon Lab company.
System the most according to claim 6, it is characterised in that described automatic gear selecting circuit is multiplexer, and model is the MAX4617 of Maxim company.
System the most according to claim 6, it is characterised in that the described OP777/AD8608/AD8627 that operational amplifier model is ADI company.
10. according to the system described in any one of claim 5-9, it is characterised in that described wireless transmission unit is NI WSN module.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113158552A (en) * | 2021-03-25 | 2021-07-23 | 中国市政工程中南设计研究总院有限公司 | Bioreactor operation condition grading prediction method and system based on time sequence |
| CN113465658A (en) * | 2021-05-24 | 2021-10-01 | 湖南大学 | Non-contact temperature measurement and material component detection device and method based on magnetic conductivity |
| CN114778642A (en) * | 2022-03-23 | 2022-07-22 | 深圳硅基传感科技有限公司 | Glucose concentration information acquisition device with three electrodes |
| CN115950938A (en) * | 2023-03-14 | 2023-04-11 | 武汉理工大学 | Detection method and circuit of electrochemical detector based on Internet of things |
-
2016
- 2016-04-01 CN CN201620271791.6U patent/CN205506113U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113158552A (en) * | 2021-03-25 | 2021-07-23 | 中国市政工程中南设计研究总院有限公司 | Bioreactor operation condition grading prediction method and system based on time sequence |
| CN113465658A (en) * | 2021-05-24 | 2021-10-01 | 湖南大学 | Non-contact temperature measurement and material component detection device and method based on magnetic conductivity |
| CN114778642A (en) * | 2022-03-23 | 2022-07-22 | 深圳硅基传感科技有限公司 | Glucose concentration information acquisition device with three electrodes |
| CN114778642B (en) * | 2022-03-23 | 2023-12-05 | 深圳硅基传感科技有限公司 | Glucose concentration information acquisition device with three electrodes |
| CN115950938A (en) * | 2023-03-14 | 2023-04-11 | 武汉理工大学 | Detection method and circuit of electrochemical detector based on Internet of things |
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