CN205484375U - Albumen spot test device - Google Patents
Albumen spot test device Download PDFInfo
- Publication number
- CN205484375U CN205484375U CN201620051531.8U CN201620051531U CN205484375U CN 205484375 U CN205484375 U CN 205484375U CN 201620051531 U CN201620051531 U CN 201620051531U CN 205484375 U CN205484375 U CN 205484375U
- Authority
- CN
- China
- Prior art keywords
- water pump
- protein
- tube
- settling tank
- trichloroacetic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses an albumen spot test device, including protein solutions case and trichloroacetic acid solution tank, the top of protein solutions case is equipped with first water pump, trichloroacetic acid solution tank's top is equipped with the third water pump, first water pump upper portion is connected with the transfer line, and the transfer line one end of keeping away from first water pump is connected with the setting tank, inside the one end of transfer line runs through the top of setting tank and extends to it, and the transfer line is connected with the connecting pipe of establishing on setting tank inner chamber upper portion. This albumen spot test device, simple structure and convenient operation have reached the effect to the protein spot test, should survey the device and through the protein solution to in protein solutions case and the trichloroacetic acid solution tank and the reaction of trichloroacetic acid solution, reach the effect that deposits protein, and the direct micropore inboard of putting the supernatant body after deposiing on the ELIASA is surveyd, reaches the effect of fast high -efficient survey protein.
Description
Technical field
This utility model relates to protein determination technical field, is specially a kind of Quick test device for protein.
Background technology
Protein is composition all cells of human body, the important component of tissue, all important composition portions of body
Divide the participation being required for protein.Generally, protein accounts for the 18% of human body all-mass, most important
Or it is relevant with biosis, protein is the material base of life, is organic macromolecule, is structure
Become the basic Organic substance of cell, be the main undertaker of vital movement, there is no life without protein.
In milk powder, protein content is an important indicator of milk powder inspection work, current domestic main employing
Conventional Kjeldahl's method, is placed directly on heating by electric cooker, digestion distillation by digestion bottle, and the most employings are often
Rule or the Kjeldahl nitrogen determination protein content in foodstuff of improvement, but this method is time-consuming and complex operation, examination
Agent consumption is big, so needing a kind of Quick test device for protein to solve problem.
Utility model content
The purpose of this utility model is to provide a kind of Quick test device for protein, to solve above-mentioned background skill
The problem proposed in art.
For achieving the above object, this utility model provides following technical scheme: a kind of albumen quickly measures dress
Putting, including protein solution case and solution of trichloroacetic acid case, the top of described protein solution case is provided with the first water
Pump, the top of described solution of trichloroacetic acid case is provided with the 3rd water pump, and described first water pump top connection has defeated
Liquid pipe, and tube for transfusion away from the first water pump one end connect have settling tank, one end of described tube for transfusion to run through
The top of settling tank also extends to inside it, and tube for transfusion and be located at the connecting tube on settling tank inner chamber top even
Connecing, connecting tube is provided with spray head, and the top of described settling tank is provided with Butterworth Hatch, described settling tank interior
Being provided with screen plate in the middle part of chamber, the top of described settling tank is interspersed is provided with the first feed tube, and the first feed tube
It is located at the one end within settling tank and penetrates filter plate and extend to below, the other end of the first feed tube
Connecting and have the second water pump, the side of described second water pump connects pipeline, and pipeline is away from the second water pump
One end connects has adapter, pipeline to be provided with branched pipe, and the lower section of described branched pipe is provided with support, and props up
Frame is provided with test tube clamp, and described test tube clamp is arranged with centrifuge tube, and the side of described support is provided with microplate reader,
The side of described adapter is connected by the second feed tube and the 3rd water pump.
Preferably, the quantity of described centrifuge tube is three, and three centrifuge tubes are equidistantly located on support, institute
State the mouth of pipe of branched pipe above the mouth of pipe correspondence of centrifuge tube, and branched pipe is provided with stop valve.
Preferably, the quantity of described spray head is five, and five spray heads are equidistantly located in connecting tube.
Preferably, described screen plate is constituted by no less than two-layer qualitative filter paper plate.
Compared with prior art, the beneficial effects of the utility model are: this utility model albumen quickly measures
Device, simple in construction and easy to operate, substituted for the technology of Kjeldahl nitrogen determination, reached albumen
The effect that matter quickly measures, this determinator is by the egg in protein solution case and solution of trichloroacetic acid case
White matter solution and the reaction of solution of trichloroacetic acid, reached the effect to protein precipitation, directly precipitation
After supernatant fluid be placed in the microwell plate in microplate reader and be measured, reach fast and efficiently measure protein
Effect, the consumption of less reagent, protect environment.
Accompanying drawing explanation
Fig. 1 is this utility model structural representation.
In figure: 1 protein solution case, 2 solution of trichloroacetic acid casees, 3 first water pumps, 4 second water pumps, 5
Three water pumps, 6 tube for transfusions, 7 settling tanks, 8 spray heads, 9 screen plates, 10 adapters, 11 branched pipes,
12 supports, 13 test tube clamps, 14 centrifuge tubes, 15 microplate reader.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, to the technology in this utility model embodiment
Scheme is clearly and completely described, it is clear that described embodiment is only this utility model one
Divide embodiment rather than whole embodiments.Based on the embodiment in this utility model, this area is common
The every other embodiment that technical staff is obtained under not making creative work premise, broadly falls into this
The scope of utility model protection.
Fig. 1, a kind of technical scheme of this utility model offer: a kind of Quick test device for protein, knot are provided
Structure is simple and easy to operate, substituted for the technology of Kjeldahl nitrogen determination, has reached survey quick to protein
Fixed effect, including protein solution case 1 and solution of trichloroacetic acid case 2, protein solution case 1 and trichloroacetic acid
Liquor box 2 is placed with protein solution and solution of trichloroacetic acid respectively, and the top of protein solution case 1 is provided with first
Water pump 3, the top of solution of trichloroacetic acid case 2 is provided with the 3rd water pump 5, and the first water pump 3 top connection has defeated
Liquid pipe 6, and tube for transfusion 6 away from the first water pump 3 one end connect have settling tank 7, one end of tube for transfusion 6
Run through the top of settling tank 7 and extend to inside it, and tube for transfusion 6 be located at settling tank 7 inner chamber top
Connecting tube connect, connecting tube is provided with spray head 8, and spray head 8 reaches to spray uniform effect, can be fast
The filtration of speed, the quantity of spray head 8 is five, and five spray heads 8 are equidistantly located in connecting tube, heavy
The top of shallow lake case 7 is provided with Butterworth Hatch, and Butterworth Hatch, after mensuration completes, is used for the impurity in settling tank 7
Being carried out, be provided with screen plate 9 in the middle part of the inner chamber of settling tank 7, screen plate 9 filters out in protein solution
Nitrogen substance, screen plate 9 is constituted by no less than two-layer qualitative filter paper plate, and the top of settling tank 7 is interspersed to be set
There is the first feed tube, and the first feed tube is located at the one end within settling tank 7 and penetrates filter plate 9 and prolong
Extending below, the other end of the first feed tube connects the second water pump 4, and the side of the second water pump 4 connects
Having a pipeline, and pipeline connects away from one end of the second water pump 4 and has adapter 10, adapter 10 is used for connecting
Pipeline and the second feed tube, pipeline is provided with branched pipe 11, and the lower section of branched pipe 11 is provided with support 12,
And support 12 is provided with test tube clamp 13, test tube clamp 13 is arranged with centrifuge tube 14, the number of centrifuge tube 14
Amount is three, and three centrifuge tubes 14 are equidistantly located on support 12, above the mouth of pipe correspondence of centrifuge tube 14
The mouth of pipe of branched pipe 11, and branched pipe 11 is provided with stop valve, the side of support 12 is provided with microplate reader 15,
The side of adapter 10 is connected by the second feed tube and the 3rd water pump 5, and this determinator is by albumen
Protein solution in liquor box 1 and solution of trichloroacetic acid case 2 and the reaction of solution of trichloroacetic acid, reach
Effect to protein precipitation, is directly placed on the microwell plate in microplate reader 15 the supernatant fluid after precipitation
Inside it is measured, reaches the fast effect efficiently measuring protein, the consumption of less reagent, protect environment.
During use, it is first turned on the switch of the first water pump 3, in protein solution case 1, first extracts protein liquid
Enter settling tank 7, spray head 8 spray uniform liquid fall on screen plate 9 filter, collect a hectolambda
Filtrate, then opens the switch of the second water pump 4, and the filtrate collected is transported in centrifuge tube 14, simultaneously
The switch of the 3rd water pump 5 is opened, extraction and the isopyknic percentage of filtrate in solution of trichloroacetic acid case 2
Ten solution of trichloroacetic acid, merge with filtrate reaction in solution of trichloroacetic acid to centrifuge tube, fully react
Rear centrifugal, Aspirate supernatant, liquid is put on the microwell plate of microplate reader 15 and observes solution in each micropore
Absorbance, finally measure the content of protein.
Embodiment the most of the present utility model, for the ordinary skill people of this area
For Yuan, it is possible to understand that can be real to these in the case of without departing from principle of the present utility model and spirit
Executing example to carry out multiple change, revise, replace and modification, scope of the present utility model is by claims
And equivalent limits.
Claims (4)
1. a Quick test device for protein, including protein solution case (1) and solution of trichloroacetic acid case (2),
The top of described protein solution case (1) is provided with the first water pump (3), described solution of trichloroacetic acid case (2)
Top be provided with the 3rd water pump (5), described first water pump (3) top connects tube for transfusion (6), and defeated
Liquid pipe (6) has settling tank (7) away from one end connection of the first water pump (3), it is characterised in that: described
One end of tube for transfusion (6) is run through the top of settling tank (7) and extends to inside it, and tube for transfusion (6)
Being connected with the connecting tube being located at settling tank (7) inner chamber top, connecting tube is provided with spray head (8), described
The top of settling tank (7) is provided with Butterworth Hatch, is provided with screen plate (9) in the middle part of the inner chamber of described settling tank (7),
The top of described settling tank (7) is interspersed is provided with the first feed tube, and the first feed tube is located at settling tank (7)
Internal one end and penetrate filter plate (9) and extend to below, the other end of the first feed tube connects to be had
Second water pump (4), the side of described second water pump (4) connects pipeline, and pipeline is away from the second water pump
(4) one end connects adapter (10), and pipeline is provided with branched pipe (11), described branched pipe (11)
Lower section be provided with support (12), and support (12) is provided with test tube clamp (13), described test tube clamp (13)
On be arranged with centrifuge tube (14), the side of described support (12) is provided with microplate reader (15), described connection
The side of device (10) is connected by the second feed tube and the 3rd water pump (5).
A kind of Quick test device for protein the most according to claim 1, it is characterised in that: described from
The quantity of heart pipe (14) is three, and three centrifuge tubes (14) are equidistantly located on support (12), institute
State and set on the mouth of pipe of branched pipe (11), and branched pipe (11) above the mouth of pipe correspondence of centrifuge tube (14)
There is stop valve.
A kind of Quick test device for protein the most according to claim 1, it is characterised in that: described spray
The quantity drenching head (8) is five, and five spray heads (8) are equidistantly located in connecting tube.
A kind of Quick test device for protein the most according to claim 1, it is characterised in that: described mistake
Filter plate (9) is constituted by no less than two-layer qualitative filter paper plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620051531.8U CN205484375U (en) | 2016-01-12 | 2016-01-12 | Albumen spot test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620051531.8U CN205484375U (en) | 2016-01-12 | 2016-01-12 | Albumen spot test device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205484375U true CN205484375U (en) | 2016-08-17 |
Family
ID=56670366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620051531.8U Expired - Fee Related CN205484375U (en) | 2016-01-12 | 2016-01-12 | Albumen spot test device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205484375U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108132326A (en) * | 2017-12-20 | 2018-06-08 | 江苏智荟生物科技有限公司 | A kind of method of the molten protein content of acid in detection hydrolyzed wheat protein |
-
2016
- 2016-01-12 CN CN201620051531.8U patent/CN205484375U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108132326A (en) * | 2017-12-20 | 2018-06-08 | 江苏智荟生物科技有限公司 | A kind of method of the molten protein content of acid in detection hydrolyzed wheat protein |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20180112 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |