CN205321010U - Freezing lever apparatus that carries of embryo - Google Patents

Abstract

The utility model belongs to the technical field of medical instruments, in particular to an embryo freezing rod loading device. The embryo freezing rod loading device of the present utility model includes a support part, a storage inner tube and an outer sleeve, the outer sleeve is vertically arranged at the bottom middle of the support part, the storage inner tube is sleeved in the outer sleeve, and Its upper end is connected to the bottom of the support part, a cavity is provided inside the support part, a liquid inlet channel communicating with the interior of the cavity is provided on the side wall of the support part, and the support part is vertically arranged inward from the top There is a placement channel corresponding to the outer sleeve, and the placement channel extends downward into the cavity. Advantages: Simple structure, easy to use, multiple embryo freezing rod devices can be combined and placed in a liquid nitrogen storage tank for freezing, which greatly increases the frozen storage capacity of the embryo freezing rods in the liquid nitrogen storage tank under the same volume, large The floor area required for frozen storage is greatly saved, and the storage cost is effectively saved.

Description

Embryo cryopreservation device

technical field

The utility model belongs to the technical field of medical instruments, in particular to an embryo freezing rod loading device.

Background technique

At present, there are many kinds of known cryocarrier systems, which can be divided into two types: cryopreservation tube type and cryopreservation rod type according to the main loading unit. The former is usually a cylindrical or partially cylindrical tubular container, one end of which is a blind end, and the other end is closed by a cap in the form of a screw thread or a buckle. This kind of container is usually relatively large, with a volume of more than 1 milliliter, and is mostly loaded and stored in a grid box on a layered carrier of a liquid nitrogen tank.

Most of the current cell cryopreservation adopts this form; the latter is a rod-shaped device, including: 1) in the form of an elongated straw-shaped container, which can freeze 1-5 hundred microliters of biological material samples; 2) round The end of the tube is further thinned and elongated, and the slender part is used for cryopreservation, which can be used for the cryopreservation of several microliters of samples; Biomaterials can be adhered to a few microliters on the sheet. The latter two forms belong to the cryopreservation of micro samples, and are widely used in the vitrification of eggs or embryos cultured in vitro. For the vitrification of eggs or embryos, there is also micro-freezing in the form of cryopreservation rings, that is, the eggs or embryos are loaded with the liquid adhered to the microwire rings, and then placed in a tubular container for protection and cryopreservation.

However, when the above-mentioned frozen carriers are frozen, they are stored in a cylindrical storage container at intervals, and the entire storage container is stored in the freezing container. The gap left between the carriers is relatively large, so that a certain volume The number of frozen carriers placed in the storage container is small, and the space utilization rate is extremely low.

Utility model content

The technical problem to be solved by the utility model is to provide an embryo freezing rod loading device, which effectively solves the problem that when the existing freezing carriers are frozen, they are all stored in a cylindrical storage container at intervals, and the entire storage container Stored in the freezer container, the gap between the carriers is relatively large, so that the number of freezer carriers placed in a storage container of a certain volume is small, and the space utilization rate is extremely low.

The technical scheme of the utility model to solve the above-mentioned technical problems is as follows: an embryo freezing rod-loading device, including a support part, a storage inner tube and an outer sleeve, the outer sleeve is vertically arranged at the bottom middle of the above-mentioned support part, and the storage inner tube sleeve It is arranged in the above-mentioned outer sleeve, and its upper end is connected to the bottom of the above-mentioned support part. There is a cavity inside the above-mentioned support part. The side wall of the above-mentioned support part is provided with a liquid inlet channel communicating with the inside of the above-mentioned cavity. The above-mentioned support part is vertical from the top to the inside. A placement channel corresponding to the outer sleeve is provided directly, and the placement channel extends downward into the cavity.

The beneficial effects of the utility model are: simple structure, easy to use, a plurality of embryo freezing rod loading devices can be combined and placed in a liquid nitrogen storage tank for freezing, greatly increasing the embryo freezing rod loading capacity of the liquid nitrogen storage tank under the same volume. The refrigerated storage capacity greatly saves the floor area required for refrigerated storage and effectively saves storage costs.

On the basis of the above technical solutions, the utility model can also be improved as follows.

Further, the inner diameter of the placement channel is equal to the inner diameter of the outer sleeve, and its height is greater than that of the outer sleeve.

The beneficial effect of adopting the above-mentioned further scheme is that when multiple embryo freezing rod devices are stacked up and down for storage, the outer sleeve of the upper embryo freezing rod device is completely inserted into the placement channel of the adjacent lower embryo freezing rod device, so that the adjacent The cooperation between the two embryo freezing rod devices is relatively tight, and the space occupied is small.

Further, the above-mentioned support part is in the shape of a cuboid, and there are multiple liquid inlet channels, which are evenly distributed on each side wall of the above-mentioned support part.

The beneficial effect of adopting the above-mentioned further solution is that the liquid enters quickly and the appearance is beautiful.

Further, the middle part of the bottom of the above-mentioned supporting part is provided with a connecting part matching the above-mentioned outer sleeve, and the above-mentioned connecting part is provided with an external thread, and the inner wall of the upper end of the above-mentioned outer sleeve is provided with an internal thread matching the external thread, and the above-mentioned outer sleeve The threaded connection is installed on the above-mentioned connection part.

The beneficial effect of adopting the above further solution is that the disassembly and assembly between the outer sleeve and the supporting part is relatively convenient, and it is convenient to take out the storage inner tube.

Further, at least three evenly distributed L-shaped connection structures are provided on the inner wall of the connection part, and an elastic ring seat is provided at the upper end of the storage inner tube, and the elastic ring seat is engaged in the connection structure.

The beneficial effect of adopting the above-mentioned further scheme is that when resuscitating the embryo, the phenomenon that the storage inner tube is ejected can be effectively avoided. Similarly, the carrier rod and the inner tube can be taken out from the cannula with only a little force.

Further, a magnetic block is embedded in the middle of the bottom of the support portion, and a magnetic sticker corresponding to the magnetic block is provided on the top of the ring seat, and the magnetic block and the magnetic sticker are magnetically adsorbed.

The beneficial effect of adopting the above further solution is to further optimize the connection and fit between the storage inner tube and the support part, so that the storage inner tube will not be bounced apart under the pressure difference.

Further, the storage inner tube mentioned above is a PE tube.

Further, the lower end of the above-mentioned elastic ring seat is provided with an auxiliary sleeve, and the above-mentioned auxiliary sleeve is sleeved outside the above-mentioned storage inner tube.

The beneficial effect of adopting the above further scheme is that when the storage inner tube is taken out, the storage inner tube can be removed by clamping the auxiliary sleeve, so as to prevent the storage inner tube from being damaged by external force.

Further, both sides of the above-mentioned supporting part are provided with hooking parts correspondingly.

Still further, the above-mentioned hooking part includes a horizontally arranged support bar and an inverted U-shaped buckle, and the above-mentioned buckle is arranged at an end of the above-mentioned support bar away from the above-mentioned hooking part.

The beneficial effect of adopting the above-mentioned further solution is that during frozen storage, the whole device can be well hung in the corresponding storage container and hooked on the corresponding hook, so that the overall structure is relatively stable, and storage will not occur. shaking etc.

Description of drawings

Fig. 1 is the sectional structure schematic diagram of the embryo freezing rod loading device of the present utility model;

Fig. 2 is the schematic cross-sectional structure diagram of the embryo freezing rod loading device of the present utility model;

Fig. 3 is an enlarged view of the structure of part A in Fig. 1;

Fig. 4 is a structural schematic diagram of the superimposed use state of the embryo freezing rod loading device of the present invention.

In the accompanying drawings, the list of parts represented by each label is as follows:

1. Supporting part, 2. Storage inner tube, 3. Outer tube, 4. Hooking part, 11. Cavity, 12. Liquid inlet channel, 13. Placement channel, 14. Magnetic block, 21. Elastic ring seat, 22 , magnetic paste, 31, connection part, 41, support bar, 42, buckle, 111, notch, 211, auxiliary sleeve, 311, connection structure.

detailed description

The principles and features of the present utility model are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the utility model, and are not used to limit the scope of the utility model.

Embodiment: As shown in Figure 1, the embryo freezing rod loading device of this embodiment includes a support part 1, a storage inner tube 2 and an outer tube 3, and the outer tube 3 is vertically arranged at the bottom middle of the above-mentioned support part 1, and the above-mentioned storage The inner tube 2 is sheathed in the above-mentioned outer tube 3, and its upper end is connected to the bottom of the above-mentioned support part 1. A cavity 11 is provided in the above-mentioned support part 1, and an inlet connected to the inside of the above-mentioned cavity 11 is provided on the side wall of the above-mentioned support part 1. The liquid channel 12 , the support part 1 is vertically provided with a placement channel 13 corresponding to the outer sleeve 3 from the top inward, and the placement channel 13 extends downward into the cavity 11 .

The inner diameter of the above-mentioned placement channel 13 is equal to the inner diameter of the above-mentioned outer sleeve 3, and its height is greater than the height of the above-mentioned outer sleeve 3, so as to ensure that the liquid nitrogen in the cavity 11 in the embryo freezing rod-carrying device of this layer can be frozen by the upper layer of the embryo freezing rod-carrying device. The bottom of the outer tube 3 enters into it, and the embryos in the upper storage inner tube 2 are frozen.

In the process of use, multiple embryo freezing rod devices are stacked up and down in order to assemble and combine, specifically, the outer sleeve 3 and the storage inner tube 2 of the upper embryo freezing rod device are completely inserted into the corresponding lower embryo freezing rod device. Placed in the channel 13 on the top of part 1, then put the combined multiple embryo freezing rod devices into the container to be frozen and stored, and the frozen liquid enters the corresponding embryo from the liquid inlet channel 12 on the side wall of each embryo freezing rod device. In the cavity 11, it enters the corresponding outer tube 3, and freezes the storage inner tube 2. When the tube needs to be taken out, the whole device is taken out from the liquid nitrogen container, and at the same time, each embryo is taken out from top to bottom for freezing. Afterwards, the remaining liquid nitrogen inside the cavity 11 of the embryo freezing and loading device is discharged from the liquid inlet channel 12, and the storage inner tube 2 is further taken out for trial use. The liquid inlet channel 12 can also effectively prevent the cavity 11 from After the liquid nitrogen inside is taken out, the supporting part 1 will be broken under the condition of large internal and external pressure difference.

In some embodiments, the above-mentioned support part 1 is in the shape of a cuboid, and can also be designed as a square or other regular shape structure. The above-mentioned liquid inlet channel 12 is provided with a plurality of them, and is evenly distributed on each side wall of the above-mentioned support part 1 On the top, it is convenient for the freezing liquid to quickly enter from multiple liquid inlet channels 12 for rapid frozen storage, and the bottom level of each liquid inlet channel 12 is higher than the embryo mark at the bottom of the storage inner tube 2 of the upper embryo freezing rod device at a height of 23 to ensure that the liquid nitrogen remaining in the chamber 11 can freeze the embryos at the bottom of the storage inner tube 2 of the upper embryo freezing rod device after taking out the stacked multiple embryo freezing rod devices from the liquid nitrogen .

As shown in Figures 1 and 3, in some embodiments, the middle part of the bottom of the above-mentioned supporting part 1 is provided with a connecting part 31 matching the above-mentioned outer sleeve 3, and the above-mentioned connecting part 31 is provided with external threads, and the upper end of the above-mentioned outer sleeve 3 The inner wall is provided with an internal thread matching the external thread, and the above-mentioned outer casing 3 is threadedly connected to the above-mentioned connecting part 31, and its disassembly and assembly are relatively simple, especially when taking out the storage inner pipe 2, only need to remove the outer casing Tube 3, just remove the storage inner tube 2.

As shown in Figures 1 and 3, in some embodiments, at least three evenly distributed L-shaped connecting structures 311 are provided on the inner wall of the connecting part 31, and an elastic ring seat 21 is provided at the upper end of the storage inner tube 2, and the elastic ring seat 21 is provided on the upper end of the storage inner tube 2. The ring seat 21 is engaged in the above-mentioned connection structure 311, because the solvent of the outer sleeve 3 is relatively small, and the pipe diameter is generally several millimeters, the space between the outer sleeve 3 and the storage inner tube 2 is small, and there is less liquid nitrogen inside, so The resulting pressure difference may cause the storage inner tube 2 to pop out of the outer sleeve 3. Therefore, when in use, the elastic ring seat 21 on the upper end of the storage inner tube 2 is pressed into the space formed between the connecting structures 311 by applying external force, so that the elastic The ring seat 21 is closely engaged with the L-shaped connection structure 311. When the tube is taken out, because the elastic ring seat 21 has elastic deformation, the storage inner tube 2 can be pulled out only with a little force.

In some embodiments, a magnetic block 14 is embedded in the middle of the bottom of the above-mentioned supporting part 1, and a magnetic sticker 22 corresponding to the magnetic block 14 is arranged on the top of the elastic ring seat 21, and the magnetic block 14 and the magnetic sticker 22 are magnetically Adsorption, so that the storage inner tube 2 can be adsorbed on the bottom of the support part 1, avoiding the situation that the elastic ring seat 21 is greatly deformed after long-term use, resulting in the situation that the storage inner tube 2 loses its clamping position and is ejected.

The above-mentioned storage inner tube 2 is a PE tube.

Preferably, the lower end of the above-mentioned elastic ring seat 21 is provided with an auxiliary sleeve 211, and the above-mentioned auxiliary sleeve 211 is sleeved outside the above-mentioned storage inner tube 2. Since the PE tube is relatively thin, when taking the tube, directly clamp the storage inner tube 2 It is possible to damage the storage inner tube 2, causing it to be unusable. In severe cases, it can injure its internal embryos. Therefore, the auxiliary sleeve 211 is provided. The inner tube 2 is brought out thereupon.

As shown in Figure 1, in some embodiments, the two sides of the above-mentioned supporting part 1 are provided with hooking parts 4 correspondingly, and the corresponding hooking parts are arranged on the inner wall of the freezer container to match the hooking parts 4, and the whole device is hung on On the hooks inside the freezer container.

Preferably, the above-mentioned hooking part 4 includes a horizontally arranged support bar 41 and an inverted U-shaped buckle 42, the above-mentioned buckle 42 is arranged on the end of the above-mentioned support bar 41 away from the above-mentioned hooking part 4, and the device after combining multiple groups Spread evenly among freezer containers for frozen storage.

As shown in FIG. 2 , in some embodiments, the cavity 11 is arranged horizontally and vertically in the support part 1 , and arc-shaped notches 111 are correspondingly provided on the inside of the cavity 11 on both sides, and between the notches 111 The placing channel 13 is formed, so that the outer sleeve 3 can be stably placed in the placing channel 13 without shaking, so that the embryo freezing effect is always kept in an optimal state.

The above descriptions are only preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model shall be included in this utility model. within the scope of protection of utility models.

Claims (10)

1. An embryo freezing rod device, characterized in that: it comprises a support portion (1), a storage inner tube (2) and an outer sleeve (3), and the outer sleeve (3) is vertically arranged on the support portion ( 1), the storage inner tube (2) is sheathed in the outer tube (3), and its upper end is connected to the bottom of the support part (1), and the support part (1) is provided with a cavity Body (11), the side wall of the support part (1) is provided with a liquid inlet channel (12) communicating with the inside of the cavity (11), and the support part (1) is vertically provided with The outer sleeve (3) corresponds to a placement channel (13), and the placement channel (13) extends downward into the cavity (11). 2. a kind of embryo freezing rod carrying device according to claim 1, is characterized in that: the inner diameter of described placement channel (13) is equal to the inner diameter of described outer casing (3), and its height is greater than described outer casing ( 3) The height. 3. A device for loading rods for embryo freezing according to claim 1, characterized in that: the support part (1) is in the shape of a cuboid, and there are multiple liquid inlet channels (12), which are evenly distributed among the on each side wall of the supporting part (1). 4. An embryo freezing rod loading device according to claim 1, characterized in that: the middle part of the bottom of the support part (1) is provided with a connecting part (31) matching the outer sleeve (3), The connecting part (31) is provided with an external thread, and the inner wall of the upper end of the outer casing (3) is provided with an internal thread matching the external thread, and the threaded connection of the outer casing (3) is installed on the connecting Part (31) on. 5. A kind of embryo freezing rod loading device according to claim 4, characterized in that: at least three evenly distributed L-shaped connecting structures (311) are arranged on the inner wall of the connecting part (31), and the storage The upper end of the inner tube (2) is provided with an elastic ring seat (21), and the elastic ring seat (21) is engaged in the connection structure (311). 6. An embryo freezing rod device according to claim 5, characterized in that: a magnetic block (14) is embedded in the middle of the bottom of the support part (1), and a magnetic block (14) is embedded in the top of the elastic ring seat (21). A magnetic sticker (22) corresponding to the magnetic block (14) is provided, and the magnetic block (14) is magnetically adsorbed to the magnetic sticker (22). 7. The embryo freezing rod loading device according to claim 6, characterized in that: the storage inner tube (2) is a PE tube. 8. An embryo freezing rod loading device according to claim 7, characterized in that: an auxiliary sleeve (211) is provided at the lower end of the elastic ring seat (21), and the auxiliary sleeve (211) is sleeved on The storage inner tube (2) is outside. 9 . The embryo freezing rod loading device according to any one of claims 1 to 8 , characterized in that: two sides of the supporting part ( 1 ) are correspondingly provided with hooking parts ( 4 ). 10. A stem-loading device for embryo freezing according to claim 9, characterized in that: the hooking part (4) includes a horizontally arranged support bar (41) and an inverted U-shaped buckle (42), the The buckle (42) is arranged at the end of the support bar (41) away from the hooking part (4).