CN205301125U - Biological aerosol particle optical detection device - Google Patents
Biological aerosol particle optical detection device Download PDFInfo
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- CN205301125U CN205301125U CN201520934106.9U CN201520934106U CN205301125U CN 205301125 U CN205301125 U CN 205301125U CN 201520934106 U CN201520934106 U CN 201520934106U CN 205301125 U CN205301125 U CN 205301125U
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Abstract
The utility model discloses a biological aerosol particle optical detection device, it includes uv light source, ultraviolet ray transport record of a lorry unit, scattering light detecting unit, glimmering light detecting unit and digital process unit, and wherein, ultraviolet ray transport record of a lorry unit is including plastic battery of lens, speculum and fellipsoidal mirror, and scattering light detecting unit, glimmering light detecting unit are respectively on the orientation of two perpendicular tos incident ultraviolet raies of fellipsoidal mirror and the unit connection of ultraviolet ray way, the digital process unit is connected with scattering light detecting unit, glimmering light detecting unit respectively, self -scattering light detecting unit and glimmering light detecting unit's detected signal is received and handles to the digital process unit. The utility model discloses can combine the two -dimentional glimmering spectral information of biological aerosol particle in order to generate three -dimensional biological identification information with the particle diameter register for easy reference to can provide abundanter information and effectual technological means for the biological nature of the biological aerosol of in -depth study.
Description
Technical field
This utility model relates to a kind of bioaerosol particle optics detecting device, particularly relate to a kind of scattered light signal and fluorescence signal that can simultaneously detect bioaerosol particle, and by the two-dimensional fluoroscopic spectrum information of two photodetectors collection bioaerosols, then be combined with Size to generate the optical detection apparatus of three dimensional biological identification information by two-dimensional fluoroscopic spectrum information.
Background technology
Bio-aerosol particles in air, such as antibacterial, fungus, pollen, virus etc., is all made up of multiple biological substance inside it, and therefore, its fluorescence spectrum is the summation after the fluorescence Spectra superposition of the internal various fluorescent materials of granule. Several main components in biologic grain, including tyrosine (tyrosine), tryptophan (tryptophan), reduced coenzyme (NADH) and riboflavin (riboflavin), single fluorescence spectrum all can be sent. Various amino acid whose maximum excitation wavelengths are between 240nm��280nm, and fluorescence emission spectrum is positioned at 280��350nm wave band; The maximum excitation wavelength of NADH is 340nm, and photoluminescence peak wavelength is positioned near 450nm; The maximum excitation wavelength of riboflavin is 450nm, and photoluminescence peak wavelength is between 515nm��565nm. By bio-aerosol particles just being carried out more comprehensively by the collection of bio-aerosol particles two dimension or multidimensional fluorescence Spectra information and effective identification.
Bio-aerosol particle detecting apparatus is the instrument of bio-aerosol particles characteristic in detection atmospheric environment, its light scattering phenomenon produced in laser beam according to bio-aerosol particles and fluorescence phenomenon and design. when the bioaerosol particle in air passes through light sensitive area with sampling air flow, produce the scattering light pulse relevant to its particle diameter and the fluorescent pulse relevant with particle fluorescence characteristic, scattering light pulse and fluorescent pulse are collected in electrooptical device by the optical system in bio-aerosol particle detecting apparatus, light pulse signal is become corresponding electric impulse signal by electrooptical device, signal processing system in bio-aerosol particle detecting apparatus is by electric impulse signal filtering and amplifies, and screen after counting process through digital processing circuit, obtain the aerosol particle subnumber of each shelves particle diameter and fluorescence.
Disclosed in December in 2011 28 days " single light source bio-aerosol particle detecting apparatus " (see patent publication No.: 102297824A), the advantage of this detecting device is: at aerated solids particle laser analyzer (see patent file, patent publication No.: CN101398367A) ellipsoidal mirror delustring part installs the method for light reflection mirror and forms double; two hot spot, detection particle calculates the aerodynamic diameter of particle by the flight time of double; two hot spots, overcomes the problem that scattered light intensity detection mode particle size can not accurately reflect particle dynamics particle size parameters.The problem that simultaneously it also avoid the fluorescence analysis error that the concentration change of detection particle causes, improves the degree of stability of detection. Adopt integration keeping method to calculate double; two fluorescence peak intensity integration simultaneously, the basis almost without increase instrument design cost is greatly improved the detection sensitivity of instrument. But the deficiency that this detecting device exists is: its fluorescence detecting system adopts the fluorescence signal that ultraviolet continuous light source is excited by single photodetector to be detected, the two dimension of bio-aerosol particles or multidimensional fluorescence Spectra bio information can not be carried out simultaneously detection analysis simultaneously, it is impossible to very comprehensively and effectively bio-aerosol particles is carried out bio-identification.
Utility model content
This utility model provides a kind of bioaerosol particle optics detecting device, in order to be combined with Size by the two-dimensional fluoroscopic spectrum information of bioaerosol particle, to generate three dimensional biological identification information.
For reaching above-mentioned purpose, the bioaerosol particle optics detecting device that this utility model provides includes: ultraviolet source, UV light path unit, scattering optical detecting unit, fluorescence detection unit and digital processing element, wherein,
Described UV light path unit includes shaping lens group, reflecting mirror and ellipsoidal mirror, wherein,
The ultraviolet light that described ultraviolet source sends enters the Spot detection district of described ellipsoidal mirror after described shaping lens group shaping collimates, and described ellipsoidal mirror is used for collecting scattering light and exciting fluorescence;
After described reflecting mirror is installed on the Spot detection district of described ellipsoidal mirror, and between the central shaft of described reflecting mirror and ultraviolet light emission direction, shape has angle to form double; two hot spot in this Spot detection district;
Described Spot detection district is L to the distance of mirror surface, and the centre-to-centre spacing of this pair of hot spot is D, described angle be sized to 1/2arctg (D/L);
Described scattering optical detecting unit, described fluorescence detection unit connect with described UV light path unit respectively from the two of described ellipsoidal mirror directions being perpendicular to incident uv;
Described digital processing element is connected with described scattering optical detecting unit, described fluorescence detection unit respectively;
Described scattering optical detecting unit is used for detecting scattered light signal, and by this scattered light signal output extremely described digital processing element to obtain Size information;
Described fluorescence detection unit is used for detecting fluorescence signal, and two-dimensional fluoroscopic spectrum information the output extremely described digital processing element of bioaerosol is obtained according to this fluorescence signal, described fluorescence detection unit includes filter plate, slit plate, shaping collimating lens, fluorescence decomposer, shaping condenser lens and two photodetectors, wherein
Described fluorescence is excited to expose to described shaping collimating lens through described slit plate after described filter plate filter out background noise,
Described shaping collimating lens excites fluorescence to be shaped as collimated light beam described in after described slit plate,
Described fluorescence decomposer excites fluorescence to decompose according to the difference of wavelength described in after being shaped as collimated light beam,
Excite fluorescence to expose to described shaping condenser lens with different angle of incidence described in different wave length, described shaping condenser lens to described excite fluorescent foci after on its focal plane, form a plurality of spectral line,
Two described photodetectors detect a plurality of described spectral line to obtain the two-dimensional fluoroscopic spectrum information of bioaerosol;
Described digital processing element receives and processes the detection signal from described scattering optical detecting unit and described fluorescence detection unit.
In an embodiment of the present utility model, described ultraviolet source can be ultraviolet laser or ultraviolet LED.
In an embodiment of the present utility model, the light beam that described reflecting mirror can be reflecting prism and the output of described shaping lens group is collimated beam, or described reflecting mirror be the light beam of spherical reflector or non-spherical reflector and the output of described shaping lens group is non-critical collimated beam.
In an embodiment of the present utility model, described fluorescence decomposer can be plane grating or dispersing prism.
In an embodiment of the present utility model, described two-dimensional fluoroscopic spectrum information is combined to generate three dimensional biological identification information by described digital processing element further with Size.
The two-dimensional fluoroscopic spectrum information of bioaerosol particle can be combined to generate three dimensional biological identification information by the bioaerosol particle optics detecting device that this utility model provides with Size such that it is able to the biological nature for furtheing investigate bioaerosol provides more rich information and effective technological means.
Accompanying drawing explanation
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the bioaerosol particle optics detecting device of this utility model one embodiment;
Fig. 2 is the index path of the UV light path unit in the bioaerosol particle optics detecting device of this utility model one embodiment.
Description of reference numerals: 11-ultraviolet source; 12-UV light path unit; 13-scattering optical detecting unit; 14-fluorescence detection unit; 15-digital processing element; 21-shaping lens group; 22-ultraviolet light; 23a-scattering light; 23b-excites fluorescence; 24-reflecting mirror; 25-ellipsoidal mirror; 41/41 '-filter plate; 42-slit plate; 43-shaping collimating lens; 44-fluorescence decomposer; 45-shaping condenser lens; 46/46 '-photodetector;
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is clearly and completely described, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole embodiments. Based on the embodiment in this utility model, the every other embodiment that those of ordinary skill in the art obtain under not paying creative work premise, broadly fall into the scope of this utility model protection.
Fig. 1 is the structural representation of the bioaerosol particle optics detecting device of this utility model one embodiment, Fig. 2 is the index path of the UV light path unit in the bioaerosol particle optics detecting device of this utility model one embodiment, as shown in the figure, the bioaerosol particle optics detecting device that this utility model provides includes: ultraviolet source 11, UV light path unit 12, scattering optical detecting unit 13, fluorescence detection unit 14 and digital processing element 15, wherein
UV light path unit 12 includes shaping lens group 21, reflecting mirror 24 and ellipsoidal mirror 25, wherein,
Ultraviolet source 11 can be ultraviolet laser or ultraviolet LED, enters the Spot detection district of ellipsoidal mirror 25 after the ultraviolet light 22 shaped battery of lens 21 shaping collimation that ultraviolet source 11 sends, and ellipsoidal mirror 25 is used for collecting scattering light 23a and exciting fluorescence 23b;
After reflecting mirror 24 is installed on the Spot detection district of ellipsoidal mirror 25, and shape has angle to form double; two hot spot in this Spot detection district between the central shaft of reflecting mirror 24 and ultraviolet light emission direction, the light beam that reflecting mirror 24 can be reflecting prism and shaping lens group 21 output is collimated beam, or reflecting mirror 24 can be the light beam of spherical reflector or non-spherical reflector and shaping lens group 21 output be non-critical collimated beam;
Spot detection district is L to the distance of mirror surface, and the centre-to-centre spacing of this pair of hot spot is D, above-mentioned angle be sized to 1/2arctg (D/L);
Scattering optical detecting unit 13, fluorescence detection unit 14 connect with UV light path unit 12 respectively from the two of ellipsoidal mirror 25 directions being perpendicular to incident uv 22;
Digital processing element 15 is connected with scattering optical detecting unit 13, fluorescence detection unit 14 respectively;
Scattering optical detecting unit 13 is used for detecting scattered light signal, and exports to digital processing element 15 this scattered light signal to obtain Size information;
Fluorescence detection unit 14 is used for detecting fluorescence signal, and obtain the two-dimensional fluoroscopic spectrum information of bioaerosol according to this fluorescence signal and export to digital processing element 15, fluorescence detection unit 14 includes filter plate 41/41 ', slit plate 42, shaping collimating lens 43, fluorescence decomposer 44, shaping condenser lens 45 and two photodetectors 46/46 ', wherein
Slit plate 42 is provided with a slit for exciting fluorescence to pass through, and exposes to shaping collimating lens 43 through slit plate 42 after exciting fluorescence sheet 41/41 ' filter out background noise after filtering,
The fluorescence that excites after slit plate 42 is shaped as collimated light beam by shaping collimating lens 43,
Fluorescence decomposer 44 can be plane grating or dispersing prism, and the fluorescence that excites after being shaped as collimated light beam is decomposed by fluorescence decomposer 44 according to the difference of wavelength,
The fluorescence that excites of different wave length exposes to shaping condenser lens 45 with different angle of incidence, shaping condenser lens 45 to forming a plurality of spectral line after exciting fluorescent foci on its focal plane,
Two photodetectors 46/46 ' detect a plurality of spectral line to obtain the two-dimensional fluoroscopic spectrum information of bioaerosol;
Digital processing element 15 receives and processes the detection signal of self-scattering optical detecting unit 13 and fluorescence detection unit 14, and two-dimensional fluoroscopic spectrum information is combined to generate three dimensional biological identification information by digital processing element 15 further with Size.
The two-dimensional fluoroscopic spectrum information of bioaerosol particle can be combined to generate three dimensional biological identification information by the bioaerosol particle optics detecting device that this utility model provides with Size such that it is able to the biological nature for furtheing investigate bioaerosol provides more rich information and effective technological means.
One of ordinary skill in the art will appreciate that: accompanying drawing is the schematic diagram of an embodiment, module or flow process in accompanying drawing are not necessarily implemented necessary to this utility model.
One of ordinary skill in the art will appreciate that: the module in device in embodiment can describe in the device being distributed in embodiment according to embodiment, it is also possible to carries out respective change and is disposed other than in one or more devices of the present embodiment. The module of above-described embodiment can merge into a module, it is also possible to is further split into multiple submodule.
Last it is noted that above example is only in order to illustrate the technical solution of the utility model, it is not intended to limit; Although this utility model being described in detail with reference to previous embodiment, it will be understood by those within the art that: the technical scheme described in previous embodiment still can be modified by it, or wherein portion of techniques feature is carried out equivalent replacement;And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of this utility model embodiment technical scheme.
Claims (5)
1. a bioaerosol particle optics detecting device, it is characterised in that including: ultraviolet source, UV light path unit, scattering optical detecting unit, fluorescence detection unit and digital processing element, wherein,
Described UV light path unit includes shaping lens group, reflecting mirror and ellipsoidal mirror, wherein,
The ultraviolet light that described ultraviolet source sends enters the Spot detection district of described ellipsoidal mirror after described shaping lens group shaping collimates, and described ellipsoidal mirror is used for collecting scattering light and exciting fluorescence;
After described reflecting mirror is installed on the Spot detection district of described ellipsoidal mirror, and between the central shaft of described reflecting mirror and ultraviolet light emission direction, shape has angle to form double; two hot spot in this Spot detection district;
Described Spot detection district is L to the distance of mirror surface, and the centre-to-centre spacing of this pair of hot spot is D, described angle be sized to 1/2arctg (D/L);
Described scattering optical detecting unit, described fluorescence detection unit connect with described UV light path unit respectively from the two of described ellipsoidal mirror directions being perpendicular to incident uv;
Described digital processing element is connected with described scattering optical detecting unit, described fluorescence detection unit respectively;
Described scattering optical detecting unit is used for detecting scattered light signal, and by this scattered light signal output extremely described digital processing element to obtain Size information;
Described fluorescence detection unit is used for detecting fluorescence signal, and two-dimensional fluoroscopic spectrum information the output extremely described digital processing element of bioaerosol is obtained according to this fluorescence signal, described fluorescence detection unit includes filter plate, slit plate, shaping collimating lens, fluorescence decomposer, shaping condenser lens and two photodetectors, wherein
Described fluorescence is excited to expose to described shaping collimating lens through described slit plate after described filter plate filter out background noise,
Described shaping collimating lens excites fluorescence to be shaped as collimated light beam described in after described slit plate,
Described fluorescence decomposer excites fluorescence to decompose according to the difference of wavelength described in after being shaped as collimated light beam,
Excite fluorescence to expose to described shaping condenser lens with different angle of incidence described in different wave length, described shaping condenser lens to described excite fluorescent foci after on its focal plane, form a plurality of spectral line,
Two described photodetectors detect a plurality of described spectral line to obtain the two-dimensional fluoroscopic spectrum information of bioaerosol;
Described digital processing element receives and processes the detection signal from described scattering optical detecting unit and described fluorescence detection unit.
2. bioaerosol particle optics detecting device according to claim 1, it is characterised in that described ultraviolet source is ultraviolet laser or ultraviolet LED.
3. bioaerosol particle optics detecting device according to claim 1, it is characterized in that, the light beam that described reflecting mirror is reflecting prism and the output of described shaping lens group is collimated beam, or described reflecting mirror be the light beam of spherical reflector or non-spherical reflector and the output of described shaping lens group is non-critical collimated beam.
4. bioaerosol particle optics detecting device according to claim 1, it is characterised in that described fluorescence decomposer is plane grating or dispersing prism.
5. bioaerosol particle optics detecting device according to claim 1, it is characterised in that described two-dimensional fluoroscopic spectrum information is combined to generate three dimensional biological identification information by described digital processing element further with Size.
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| CN201520934106.9U CN205301125U (en) | 2015-11-20 | 2015-11-20 | Biological aerosol particle optical detection device |
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| CN201520934106.9U CN205301125U (en) | 2015-11-20 | 2015-11-20 | Biological aerosol particle optical detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN113916783A (en) * | 2021-10-13 | 2022-01-11 | 丹东百特仪器有限公司 | Optical method for detecting toxic substances based on time flight method |
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2015
- 2015-11-20 CN CN201520934106.9U patent/CN205301125U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN113916783A (en) * | 2021-10-13 | 2022-01-11 | 丹东百特仪器有限公司 | Optical method for detecting toxic substances based on time flight method |
| CN113916783B (en) * | 2021-10-13 | 2023-11-24 | 丹东百特仪器有限公司 | Optical method for detecting toxic substances based on time-of-flight method |
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