CN205295305U - Chip is caught to microfluid cell - Google Patents
Chip is caught to microfluid cell Download PDFInfo
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- CN205295305U CN205295305U CN201521070750.2U CN201521070750U CN205295305U CN 205295305 U CN205295305 U CN 205295305U CN 201521070750 U CN201521070750 U CN 201521070750U CN 205295305 U CN205295305 U CN 205295305U
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Abstract
The utility model discloses a chip is caught to microfluid cell, at least, including clear glass basement and clear glass cover plate, a clear glass basement side is fluted along the length direction sculpture, grooved underside is the inclined plane, the last the biggest one side of the degree of depth of grooved underside is equipped with the entry, an export has been seted up at least to the minimum one side of the last degree of depth of grooved underside, the depth capacity of recess is 30 -200um, and grooved underside's the minimum degree of depth is not more than the size of at least a target cell, the clear glass basement offer notched side and a side of clear glass cover plate fixed hug closely as an organic whole. This chip simple structure, convenient operation can accurately catch not unidimensional target cell fast.
Description
Technical field
This utility model relates to cell capture technical field of tools, is specifically related to a kind of Microfluidic cell and catches chip, and this Microfluidic cell is caught chip and be can be used as the instrument of diagnosing tumor, auxiliary treatment and biochemical analysis research.
Background technology
For many cancer patients, its death causes mainly due to metastatic tumor. When after patient's excision primary tumor, current lesion detection means are difficult to reaction treatment situation in time, identify metastatic tumor, instruct follow-up chemicotherapy process, thus causing that patient misses best occasion for the treatment and cannot timely and effective adjustment futile treatment and therapeutic regimen. It is unable to the metastatic tumor that successful treatment has more, causes that patient is finally dead. It seems that from Point of View of Clinical, metastatic tumor can be regarded as the concluding event in cancer organic growth process.
The anxious atraumatic process to be developed of people extracts the instrument of tumor cells specimens from patient.
Circulating tumor cell (CTCs) refers to the solid-cell alive existed in blood with extremely low level. Along with deepening continuously that circulating tumor cell is studied, it is enriched with and identifies that these cells have become as a kind of method of auxiliary cancer diagnosis. Regulator (such as FDA) have been approved by some catch based on circulating tumor, the clinical practice of identification systems.
In order to separate and be enriched with in body fluid circulating tumor cell and diffusion tumor cell, people utilize the feature such as this kind of cell size, surface molecular expression develop some catch accordingly, enrichment method. Such as, patent CN102925337B provides a kind of Microfluidic cell and catches chip, the weak point of this CN102925337B is to be plugged with the steel disc of thickness in two panels hard material, enable to become the wedge shape raceway groove gradually changed, polydimethylsiloxane is coated again in the surrounding of chip, dry, make sample can not flow out from surrounding. But the structure that this Microfluidic cell catches chip is complicated, and complicated process of preparation, yield rate is relatively low.
Summary of the invention
In order to solve prior art Problems existing, this utility model provides a kind of Microfluidic cell and catches chip, and this chip structure is simple, easy to operate, can catch various sizes of target cell rapidly and accurately.
Realizing the technical scheme that this utility model above-mentioned purpose samples is:
A kind of Microfluidic cell catches chip, at least include clear glass substrate and clear glass cover plate, clear glass substrate one side is etched with groove along its length, groove floor is inclined-plane, the side that in groove floor, the degree of depth is maximum is provided with entrance, the side that in groove floor, the degree of depth is minimum at least offers an outlet, the depth capacity of groove is 30-200um, and the minimum-depth of groove floor is not more than the size of at least one target cell, clear glass substrate offers reeded side, and fixing being close to is integrated with the one side of clear glass cover plate.
Described groove is wedge shaped.
The width of the side that depth of groove is maximum is the narrowest, and the minimum side width of depth of groove is the widest.
The width of groove is 0.1��200 millimeter.
The middle part of the entrance side that to be positioned in groove floor the degree of depth maximum, the side that in groove floor, the degree of depth is minimum is provided with guiding gutter, and outlet has three, and two of which outlet lays respectively in the two ends of guiding gutter, and another outlet is positioned at the middle part of guiding gutter.
The thickness of described clear glass substrate and clear glass cover plate is 0.1��10 millimeter.
Compared with prior art, the beneficial effects of the utility model and advantage are in that:
This chip structure is simple, and processing technology is simple, and cost of manufacture is cheap, and operation is very easy, can carry out various sizes of cell rapidly and efficiently separating, being enriched with.
Accompanying drawing explanation
Fig. 1 catches the structural representation of chip for the Microfluidic cell that this utility model provides.
Fig. 2 is the structural representation of clear glass substrate.
Fig. 3 utilizes this Microfluidic cell to catch chip to catch the microscope figure of target cell.
Wherein, 1-clear glass substrate, 2-clear glass cover plate, 3-groove, 4-entrance, 5-guiding gutter, 6-export.
Detailed description of the invention
Below in conjunction with accompanying drawing, this utility model is described in detail.
The Microfluidic cell that this utility model provides catches the structure of chip as it is shown in figure 1, this cell capture chip includes clear glass substrate 1 and clear glass cover plate 2, and the thickness of clear glass substrate and clear glass cover plate is 0.1��10 millimeter.
The one side of clear glass substrate 1 is etched with the groove 3 of wedge shape along its length, and the width of groove 3 is 0.1��200 millimeter. The width of the side that groove 3 degree of depth is maximum is the narrowest, and the minimum side width of groove 3 degree of depth is the widest. A degree of depth maximum middle side part in groove 3 bottom surface is provided with entrance 4, it is semicircular guiding gutter 5 that the degree of depth minimum side in groove 3 bottom surface is provided with cross section, the diameter of guiding gutter cross section is 1.0 millimeters, the middle part of guiding gutter 5 and two ends are equipped with outlet 6, in the present embodiment, outlet and entrance are manhole. It should be noted that, the depth capacity of groove is 30-200um, the minimum-depth of groove 3 bottom surface is not more than the size of at least one target cell, if the minimum-depth of groove floor is more than any target cell size, so this target cell will directly flow out from outlet, it is impossible to catches. In the present embodiment, the width of the side that depth of groove is maximum is 9mm, and the minimum side width of the degree of depth is 19, and the depth capacity of groove is 60um.
Clear glass substrate 1 offers reeded side, and fixing being close to is integrated with the one side of clear glass cover plate 2, the purpose of do so is to make microfluid (fluid containing target cell) can not flow out from the gap between clear glass substrate and clear glass cover plate, can only flow out from outlet, it is simple to catch target cell.
This Microfluidic cell catches chip, and to catch the principle of cell as follows:
Owing to groove is wedge shaped, the distance of groove floor difference place place and clear glass cover plate constantly changes, namely the space that groove floor is formed with transparent glass cover sheet from the inlet to the outlet constantly diminishes, when human fluid's sample flows into wedge-shaped impression by entrance, due to limited in space, the target cell flowed into human fluid's sample will block in specific position, thus various sizes of target cell can be caught, finally make various sizes of target cell realize being automatically separated and being enriched with when by groove.
Utilizing this Microfluidic cell to catch chip, to catch the method for target cell as follows:
1) human fluid's sample to be detected is injected from the entrance of chip;
2) phosphate buffered solution (i.e. PBS solution) is injected from the entrance of chip, with the speed of 100ul/min, groove is carried out;
3) by probe material (as: contaminate nuclear fluorescent dye such as DAPI or Hoechst dyestuff) or immunoreagent inject from the entrance of chip, target cell is identified;
4) again phosphate buffered solution (i.e. PBS solution) is injected from the entrance of chip, groove is carried out;
5) chip is placed in the target cell that basis of microscopic observation is caught.
Capture effect is analyzed:
As shown in Figure 3, select region one, region two, region three and observation area, four, region respectively, it can be seen that target separation in said chip and enrichment, the distribution of the target cell observed from aforementioned four observation area is it can be seen that the separation of target cell and enrichment are the size of target cell and the size of wedge-shaped impression interacts result.
The manufacture method that the Microfluidic cell that this utility model provides catches chip is as follows:
1) clear glass selecting two panels thickness to be 0.1��10mm (being generally 1.1mm), wherein a piece of as clear glass substrate, another sheet is as clear glass cover plate;
2) one side of clear glass substrate is carried out mask, the side of clear glass substrate overlay film exposes the trapezoid area needing etching;
3) wet etching, is submerged etching liquid (Guangzhou Mei Di new glass Materials Co., Ltd in the region needing etching, can also replace with other commercial etching liquids) in perform etching (lithographic method adopts the method described in Chinese patent 201410574398.X), form wedge-shaped impression;
4) carving cross section at the side carving machine that the wedge-shaped impression degree of depth is minimum is semicircular guiding gutter;
5) either side that clear glass substrate is offered reeded side and clear glass cover plate carries out water bonding, treats water volatilization completely, carries out high temperature sintering, and clear glass substrate becomes to fuse into clear glass cover plate and is integrated;
6) punch on the lateral surface of clear glass substrate, offer entrance and exit.
Claims (6)
1. a Microfluidic cell catches chip, at least include clear glass substrate and clear glass cover plate, it is characterized in that: the one side of clear glass substrate is etched with groove along its length, groove floor is inclined-plane, the side that in groove floor, the degree of depth is maximum is provided with entrance, the side that in groove floor, the degree of depth is minimum at least offers an outlet, the depth capacity of groove is 30-200um, and the minimum-depth of groove floor is not more than the size of at least one target cell, clear glass substrate offers reeded side, and fixing being close to is integrated with the one side of clear glass cover plate.
2. Microfluidic cell according to claim 1 catches chip, it is characterised in that: described groove is wedge shaped.
3. Microfluidic cell according to claim 2 catches chip, it is characterised in that: the width of the side that depth of groove is maximum is the narrowest, and the minimum side width of depth of groove is the widest.
4. Microfluidic cell according to claim 2 catches chip, it is characterised in that: the width of groove is 0.1��200 millimeter.
5. Microfluidic cell according to claim 1 catches chip, it is characterized in that: the middle part of the entrance side that to be positioned in groove floor the degree of depth maximum, the side that in groove floor, the degree of depth is minimum is provided with guiding gutter, outlet has three, two of which outlet lays respectively in the two ends of guiding gutter, and another outlet is positioned at the middle part of guiding gutter.
6. Microfluidic cell according to claim 1 catches chip, it is characterised in that: the thickness of described clear glass substrate and clear glass cover plate is 0.1��10 millimeter.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015109194401 | 2015-12-11 | ||
| CN201510919440 | 2015-12-11 |
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| Publication Number | Publication Date |
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| CN205295305U true CN205295305U (en) | 2016-06-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201521070750.2U Active CN205295305U (en) | 2015-12-11 | 2015-12-21 | Chip is caught to microfluid cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108517291A (en) * | 2018-06-01 | 2018-09-11 | 东莞东阳光科研发有限公司 | A kind of cell capture device |
| CN109550531A (en) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | A kind of micro-fluidic chip that magnetism size relies on |
-
2015
- 2015-12-21 CN CN201521070750.2U patent/CN205295305U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108517291A (en) * | 2018-06-01 | 2018-09-11 | 东莞东阳光科研发有限公司 | A kind of cell capture device |
| CN109550531A (en) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | A kind of micro-fluidic chip that magnetism size relies on |
| CN109550531B (en) * | 2019-01-28 | 2021-09-07 | 武汉纺织大学 | Micro-fluidic chip with dependent magnetic size |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200507 Address after: 430000 B7, C and D units, one or two floors, medical equipment Park, 818 hi tech Avenue, East Lake New Technology Development Zone, Hubei, Wuhan, Patentee after: Wuhan Lanting intelligent Medicine Co., Ltd Address before: 430200 Hubei Province, Wuhan city Jiangxia District an even Highway No. 1 Patentee before: Wuhan Textile University |