CN205263036U - System for fluorouracil in short -term test blood specimen - Google Patents
System for fluorouracil in short -term test blood specimen Download PDFInfo
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- CN205263036U CN205263036U CN201521047066.2U CN201521047066U CN205263036U CN 205263036 U CN205263036 U CN 205263036U CN 201521047066 U CN201521047066 U CN 201521047066U CN 205263036 U CN205263036 U CN 205263036U
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- sample
- detector
- fluorouracil
- peristaltic pump
- thermocolumn
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Abstract
The utility model relates to a system for fluorouracil in short -term test blood specimen, including serum sample preprocessor module and detection module, be equipped with dress sample cup, spiral appearance and centrifugal separator in the serum sample preprocessor module, the dress sample cup place on the spiral appearance, and the spiral appearance is placed by the centrifugal separator, is equipped with automatic quantitative injector, reservoir, high -pressure peristaltic pump, system pressure table, sample waste liquid collector, constant temperature column temperature case, detector, system's waste liquid collector and computer in the detection module, reservoir and high -pressure peristaltic pump connection, the quantitative injector of automation be connected with high -pressure peristaltic pump, sample waste liquid collector and constant temperature column temperature case, constant temperature column temperature case connect detector and system's waste liquid collector, tester electricity linked computers. The utility model discloses check -out time has only 15 -20 minute, greatly reduced detect the required time.
Description
Technical field
The utility model relates to the detection technique of fluorouracil in a kind of blood sample, relates in particular to the system of fluorouracil in a kind of fast detecting blood sample.
Background technology
Fluorouracil (5-fluorouracil, be called for short 5-FU) is the fluoride of the uracil that synthesized by Heidelberger nineteen fifty-seven, is one of common anti-metabolism chemotherapeutic. Because 5-FU is cheap, significantly interference, blocking dna, RNA and protein are synthetic again in vivo, and tumor in digestive tract and other solid tumors are had to good efficacy, put into practice so 5-FU is widely used in clinical chemotherapy.
Correlative study both at home and abroad shows: because the activity of patient body intracellular metabolite enzyme there are differences, individuality differs greatly to the metabolic rate of 5-FU. In the time that body surface area calculates the consumption of 5-FU routinely, more difficult grasp dosage. Same dosage affects the treatment because blood concentration is on the low side, or often has generation because of the higher phenomenon that can not tolerate of blood concentration. As can be seen here, carry out the testing of 5-FU blood concentration and adjust 5-FU dosage for individuation, thereby further improve the curative effect of combined chemotherapy, and reduce as far as possible having great importance of bad reaction.
But existing detection system institute spended time is long, can increase like this generation of combined chemotherapy bad reaction.
Summary of the invention
The utility model is for above deficiency, provide a kind of accurate detection, and the fast detecting blood sample that can reduce detection time in the system of fluorouracil.
The technical scheme that the utility model adopts is:
The system of fluorouracil in a kind of fast detecting blood sample, comprise blood serum sample pretreatment module and detection module, in blood serum sample pretreatment module, be provided with dress sample cup, spiral instrument and centrifuge, dress sample cup is placed on spiral instrument, spiral instrument is placed on by centrifuge, in detection module, be provided with automatic quantitative sample device, reservoir, high pressure peristaltic pump, system pressure table, sample liquid waste collector, thermocolumn incubator, detector, system liquid waste collector and computer, described reservoir is connected with high pressure peristaltic pump, described automatic quantitative sample device is connected with high pressure peristaltic pump, sample liquid waste collector and thermocolumn incubator, described thermocolumn incubator connection detector and system liquid waste collector, described detector electrical connection computer.
As preferably, described thermocolumn incubator is in-built chromatographic column.
As preferably, described detector is ultraviolet detector.
The beneficial effects of the utility model are:
Only have 15-20 minute detection time, greatly reduce the required time of detecting.
Brief description of the drawings
Fig. 1 is structured flowchart of the present utility model.
Fig. 2 is that the utility model does not have fluorouracil and interior target blood examination design sketch.
Fig. 3 is that the utility model has fluorouracil and interior target blood examination design sketch.
In figure 1, dress sample cup, 2, spiral instrument, 3, centrifuge, 4, automatic quantitative sample device, 5, high pressure peristaltic pump, 6, reservoir, 7, system pressure table, 8, sample liquid waste collector, 9, thermocolumn incubator, 10, detector, 11, system liquid waste collector, 12, computer.
Detailed description of the invention:
As shown in Figure 1, the system of fluorouracil in a kind of fast detecting blood sample, comprise blood serum sample pretreatment module and detection module, in blood serum sample pretreatment module, be provided with dress sample cup 1, spiral instrument 2 and centrifuge 3, in blood serum sample pretreatment module, be provided with dress sample cup 1, spiral instrument 2 and centrifuge 3, dress sample cup 1 is placed on spiral instrument 2, it is other that spiral instrument 2 is placed on centrifuge 3, in detection module, be provided with automatic quantitative sample device 4, reservoir 6, high pressure peristaltic pump 5, system pressure table 7, sample liquid waste collector 8, thermocolumn incubator 9, detector 10, system liquid waste collector 11 and computer 12, described reservoir 6 is connected with high pressure peristaltic pump 5, automatic quantitative sample device 4 is connected with high pressure peristaltic pump 5, sample liquid waste collector 8 and thermocolumn incubator 9, thermocolumn incubator 9 connection detectors 10 and system liquid waste collector 11, detector 10 is electrically connected computer 12, thermocolumn incubator 9 is in-built chromatographic column, detector 10 is ultraviolet detector.
When detection, first quantitatively extract serum sample 300ul in 1 li, dress sample cup, after adding interior mark liquid 50ul, mix by 2 li of spirals of spiral instrument, then add pretreating reagent 200ul, spiral reaction 5min, then can be stand-by with 4000r/min low-speed centrifugal 5min by centrifuge 3.
After processing, the supernatant of centrifugal acquisition adds automatic quantitative sample device 4 interpolation pipes, through the analysis of special color spectra system sample introduction. The special analytical column of chromatographic system is the alkyl linked fixing phase of trifunctional C18, adopt end group sealing technical finesse free silica hydroxyl, the working solution of system operation is the mixed solution (A: B=95: 5 of water-soluble working solution A and alcohol dissolubility working solution B, v/v), system operation flow velocity is set as 0.2ml/min, running temperature is set as 20 DEG C, and detector 10 is UV-detector, and detection wavelength is 265nm. The whole analytic process of detection module only needs 5min to complete.
As shown in accompanying drawing 2,3, system performance parameter: the detection of native system is limited to 0.1ug/ml, quantitative linearity scope is 0.5-50ug/m1, quantitative approach is internal standard method, calibration curve is y=0.0848x-0.0013 (r=0.9998, wherein y is fluorouracil and the ratio of the peak area of interior mark signal, the blood concentration that x is fluorouracil). The rate of recovery experiment that native system detects is investigated and is respectively 106.2%, 98.9% and 96.7% by high, medium and low three concentration in the range of linearity, and quantitative precision is good.
The above; it is only detailed description of the invention of the present utility model; but protection domain of the present utility model is not limited to this; any those of ordinary skill in the art are in the disclosed technical scope of the utility model; the variation that can expect easily or replacement, within all should being encompassed in protection domain of the present utility model. Therefore, protection domain of the present utility model should be as the criterion with the protection domain that claims were limited.
Claims (3)
1. the system of fluorouracil in a fast detecting blood sample, it is characterized in that: comprise blood serum sample pretreatment module and detection module, in blood serum sample pretreatment module, be provided with dress sample cup, spiral instrument and centrifuge, dress sample cup is placed on spiral instrument, spiral instrument is placed on by centrifuge, in detection module, be provided with automatic quantitative sample device, reservoir, high pressure peristaltic pump, system pressure table, sample liquid waste collector, thermocolumn incubator, detector, system liquid waste collector and computer, described reservoir is connected with high pressure peristaltic pump, described automatic quantitative sample device is connected with high pressure peristaltic pump, sample liquid waste collector and thermocolumn incubator, described thermocolumn incubator connection detector and system liquid waste collector, described detector electrical connection computer.
2. the system of fluorouracil in a kind of fast detecting blood sample according to claim 1, is characterized in that: thermocolumn incubator is in-built chromatographic column.
3. the system of fluorouracil in a kind of fast detecting blood sample according to claim 1, is characterized in that: detector is ultraviolet detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521047066.2U CN205263036U (en) | 2015-12-14 | 2015-12-14 | System for fluorouracil in short -term test blood specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521047066.2U CN205263036U (en) | 2015-12-14 | 2015-12-14 | System for fluorouracil in short -term test blood specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205263036U true CN205263036U (en) | 2016-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521047066.2U Expired - Fee Related CN205263036U (en) | 2015-12-14 | 2015-12-14 | System for fluorouracil in short -term test blood specimen |
Country Status (1)
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| CN (1) | CN205263036U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113945554A (en) * | 2021-10-15 | 2022-01-18 | 中国科学院合肥物质科学研究院 | SERS detection method of antitumor drug 5-FU in serum |
-
2015
- 2015-12-14 CN CN201521047066.2U patent/CN205263036U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113945554A (en) * | 2021-10-15 | 2022-01-18 | 中国科学院合肥物质科学研究院 | SERS detection method of antitumor drug 5-FU in serum |
| CN113945554B (en) * | 2021-10-15 | 2023-08-18 | 中国科学院合肥物质科学研究院 | SERS detection method for antitumor drug 5-FU in serum |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160525 Termination date: 20181214 |