A kind of accurately brain tissue mould of drawing materials
Technical field
The utility model belongs to life science and uses device field, relates to the mould that a kind of medical research zoopery is used, and particularly relates to a kind of accurate tissue sampling mould using for animal brain.
Background technology
In medical research zoopery, often relate to cutting of brain tissue, the method cutting is mainly according to the three-dimensional elements of a fix, and crown, level and sagittal cut, and conventional is crown cutting at present, is cutting the position that cuts again concrete needs after a certain section of tissue. But in process, there are some problems cutting: (1) is cutting in the judgement of position and scope has certain subjectivity, makes to cut position inaccuracy, brings error effect to further research; (2) a common system experimentation is jointly to be completed by several experimenters, and different personnel get difference between tissue can affect comparativity, correctness and the accuracy of experimental result.
Summary of the invention
In order to overcome the problem of tissue sampling accuracy and poor accuracy in prior art, the utility model discloses a kind of accurately brain tissue mould of drawing materials, aim to provide a kind of accuracy that improves drawing materials tissue, uniformity and in the situation that reducing histone destructiveness realization accurately draw materials.
The utility model is achieved by the following technical solution:
A kind of accurately brain tissue mould of drawing materials, comprise objective table, some cutting knife gathering sills that described objective table is provided with and for the scale engineer's scale of auxiliary precise cutting, below described objective table, be provided with objective table fixed border, described objective table and objective table fixed border surround the cavity for placing trash ice. The width of cutting knife gathering sill and height are being advisable with permission cutting knife sliding cutting by fixed cutting tool.
As a kind of preferred embodiment, be provided with some cutting knife gathering sills at described objective table upper surface, be provided with scale engineer's scale at described objective table lower surface.
Certainly, when cutting object and being different animals brain, need the size of objective table and fixed border to readjust design, this distortion for those skilled in the art be apparent, all belong to protect object of the present utility model.
Preferably, described objective table and objective table fixed border are combined to form cuboid, can certainly be other structures such as cylinder, triangular prism, pentagonal prism.
In order further accurately to organize and to cut work, be provided with 2n+1 bar vertical scale engineer's scale and at least 1 horizontal scale engineer's scale at described objective table lower surface, wherein, n >=1; The equidistant parallel distribution of described vertical scale engineer's scale, described horizontal scale engineer's scale is parallel with the first horizontal cutting knife gathering sill. While adjusting the position of brain tissue interval on objective table, the center line of unit to be cut is overlapped with middle vertical scale engineer's scale (that is: n+1 article of vertical scale engineer's scale).
In the time cutting rat brain anterior cingutate cortex kiss sidepiece, can be provided with at least 4 cutting knife gathering sills at described objective table upper surface, 2 is the inclined cutter gathering sill of symmetrical inclination, all the other are the horizontal cutting knife gathering sill being parallel to each other. Preferably, described objective table upper surface is provided with 5 cutting knife gathering sills, be respectively the first inclined cutter gathering sill, the second inclined cutter gathering sill, the first horizontal cutting knife gathering sill, the second horizontal cutting knife gathering sill and the 3rd horizontal cutting knife gathering sill, described first and second inclined cutter gathering sill respectively with the crossing formation of second and third horizontal cutting knife gathering sill isosceles trapezoid, the upper and lower end of described isosceles trapezoid, is formed by second and third horizontal cutting knife gathering sill respectively, and the described first horizontal cutting knife gathering sill is positioned at isosceles trapezoid outside. Brain tissue interval is placed on objective table, adjust its position, cortex can be fixed in the first horizontal cutting knife gathering sill by cutting knife just, cutting knife snaps in one by one in first and second inclined cutter gathering sill and second and third horizontal cutting knife gathering sill tissue is cut, isosceles trapezoid region is exactly the required rat brain anterior cingutate cortex kiss sidepiece unit cutting, avoid cutting Brain shift or distortion in process, ensured that tangent plane is neat and smooth and thin and thick is even.
Certainly, while cutting different IPs group or unit, need the distribution of cutting knife gathering sill and scale engineer's scale to readjust design, this distortion for those skilled in the art be apparent, all belong to protect object of the present utility model.
In the time accurately cutting rat brain amygdaloid nucleus and organize, be provided with 3 horizontal cutting knife gathering sills parallel with horizontal scale engineer's scale at described objective table upper surface, at least 2 with the n+1 article of longitudinal cutter gathering sill that vertical scale engineer's scale is parallel.
Preferably, described objective table upper surface is provided with 4 longitudinal cutter gathering sills, is symmetrically distributed in n+1 article of vertical scale engineer's scale both sides. Brain tissue interval is placed on objective table, adjust its position, cortex can be fixed in the first horizontal cutting knife gathering sill by cutting knife just, the line of demarcation of brain tissue the two cerebral hemispheres overlaps with n+1 article of vertical scale engineer's scale, cutting knife snaps in one by one in horizontal cutting knife gathering sill and longitudinal cutter gathering sill tissue is cut, and it is exactly the required rat brain amygdaloid nucleus tissue cutting that horizontal cutting knife gathering sill and longitudinal cutter gathering sill surround less rectangular region.
Preferably, described cutting knife gathering sill width is 0.1 ~ 0.2mm, is highly 0.1 ~ 0.2mm, and in cutting knife gathering sill, has opaque paint coat.
For operation more accurately, described objective table and objective table fixed border can be water white transparency material.
The beneficial effects of the utility model are:
(1) the utility model adopts the structure with objective table fixed border, and the process of drawing materials can be operated on trash ice, has both reduced the destructiveness of organizing internal protein, makes again objective table be difficult for mobile;
(2) cut under assisting of groove and scale engineer's scale at tissue, even the asynchronism(-nization) that can make cut tissue cut, personnel's difference, all can get more accurate and consistent tissue.
Brief description of the drawings
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of the utility model embodiment 1 accurate tissue sampling mould.
Fig. 2 is the top view of objective table in the accurate tissue sampling mould of the utility model embodiment 1.
Fig. 3 is the upward view of objective table in the accurate tissue sampling mould of the utility model embodiment 1.
Fig. 4 is the top view of objective table in the accurate tissue sampling mould of the utility model embodiment 2.
In Fig. 1-3: 1. objective table, 2. objective table fixed border, 3. cutting knife gathering sill, 31. first inclined cutter gathering sills, 32. second inclined cutter gathering sills, 33. first horizontal cutting knife gathering sills, 34. second horizontal cutting knife gathering sills, 35. the 3rd horizontal cutting knife gathering sills, 4. scale engineer's scale, 41. vertical scale engineer's scales, 42. horizontal scale engineer's scales.
In Fig. 4: 1. objective table, 2. objective table fixed border, 3. cutting knife gathering sill, 31. first longitudinal cutter gathering sills, 32. first longitudinal cutter gathering sills, 33. first longitudinal cutter gathering sills, 34. first longitudinal cutter gathering sills, 35. first horizontal cutting knife gathering sills, 36. first horizontal cutting knife gathering sills, 37. first horizontal cutting knife gathering sills, 4. scale engineer's scale, 41. vertical scale engineer's scales, 42. horizontal scale engineer's scales.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiment. Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtaining under creative work prerequisite, all belong to the scope of the utility model protection.
Embodiment 1.
As Figure 1-3, a kind of mould that accurately cuts rat brain anterior cingutate cortex kiss sidepiece tissue, comprise the objective table 1 of 55mm × 30mm and be arranged on the objective table fixed border 2 of objective table 1 below, will objective table 1 and objective table fixed border 2 is fixing the cuboid (55mm × 30mm × 18mm) of cavity in forming, objective table 1 upper surface is provided with the cutting knife gathering sill 3 that has blacking coat in five grooves, be respectively the first inclined cutter gathering sill 31, the second inclined cutter gathering sill 32, the first horizontal cutting knife gathering sill 33, the second horizontal cutting knife gathering sill 34 and the 3rd horizontal cutting knife gathering sill 35, wherein, first, two inclined cutter gathering sills are 37mm × 0.1mm, first, two, three horizontal cutting knife gathering sills are 40mm × 0.1mm, first, two inclined cutter gathering sills 31 and 32 are respectively with second, three horizontal cutting knife gathering sills 34 and 35 intersect formation isosceles trapezoid, the first horizontal cutting knife 33 gathering sills are positioned at isosceles trapezoid outside, and the distance between the second horizontal cutting knife gathering sill 34 is 0.6mm, objective table 1 lower surface is provided with three vertical scale ratio 41 chis and a horizontal scale engineer's scale 42, the equidistant parallel distribution of vertical scale engineer's scale 41, and horizontal scale engineer's scale 42 is parallel with the first horizontal cutting knife gathering sill 33.
When use, mould is inserted to trash ice, make to be full of in objective table lower cavity trash ice (reach like this and be difficult for mobile effect), rat cerebral tissue's interval is placed on objective table, adjust its position, cortex upper limb can be fixed in the first horizontal cutting knife gathering sill by cutting knife just, and the line of demarcation of brain tissue the two cerebral hemispheres overlaps with middle vertical scale engineer's scale, cutting knife snaps in second one by one, three horizontal cutting knife gathering sills and first, in two inclined cutter gathering sills, rat brain anterior cingutate cortex kiss sidepiece tissue is cut, isosceles trapezoid region is exactly the required rat brain anterior cingutate cortex kiss sidepiece tissue cutting, Brain shift or distortion in process are avoided cutting, ensure that tangent plane is neat and smooth and thin and thick is even.
Embodiment 2.
As shown in Figure 4, a kind of mould that accurately cuts rat brain amygdaloid nucleus tissue, comprise the objective table 1 of 55mm × 30mm and be arranged on the objective table fixed border 2 of objective table 1 below, objective table 1 and objective table fixed border 2 are fixed together and have the cuboid (55mm × 30mm × 18mm) of cavity in forming, objective table 1 upper surface is provided with the cutting knife gathering sill that has blacking coat in seven grooves, is respectively first, two, three, four longitudinal cutter gathering sills 31, 32, 33, 34, the first, two, three horizontal cutting knife gathering sills 35, 36, 37, wherein, first, two, three, four longitudinal cutter gathering sills are 37mm × 0.2mm, first, two, three horizontal cutting knife gathering sills are 40mm × 0.2mm, and the first longitudinal cutter gathering sill 31 is 4.4mm with middle vertical scale engineer's scale distance, and the second longitudinal cutter gathering sill 32 is 3.2mm with middle vertical scale engineer's scale distance, and the 3rd, four cutting knife gathering sills 33 and 34 are taking middle vertical scale engineer's scale as symmetry axis and first, two longitudinal cutter gathering sills 31 and 32 are symmetrical arranged, the first horizontal cutting knife gathering sill 35 is 0.4mm with the distance of horizontal scale engineer's scale 42, the second horizontal cutting knife gathering sill 36 is 7.4mm with the distance of horizontal scale engineer's scale 42, and the 3rd horizontal cutting knife gathering sill 37 is 9mm with the distance of horizontal scale engineer's scale 42.
When use, mould is inserted to trash ice, make to be full of trash ice in objective table lower cavity, rat cerebral tissue's interval is placed on objective table, adjust its position, cortex upper limb can be fixed in the first horizontal cutting knife gathering sill by cutting knife just, and the line of demarcation of brain tissue the two cerebral hemispheres overlaps with middle vertical scale engineer's scale, cutting knife snaps in first one by one, two, three horizontal cutting knife gathering sills and first, two, three, in four longitudinal cutter gathering sills, rat brain amygdaloid nucleus tissue is cut, second, three horizontal cutting knife gathering sills are respectively with first, two cutting knife gathering sills and the 3rd, the rectangular region that four longitudinal cutter gathering sills surround is exactly the required rat brain amygdaloid nucleus tissue cutting, Brain shift or distortion in process are avoided cutting, ensure that tangent plane is neat and smooth and thin and thick is even.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all within spirit of the present utility model and principle, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.