CN205175959U - A many V -arrangements pipe is an appearance ware upward for albumen runs glues - Google Patents
A many V -arrangements pipe is an appearance ware upward for albumen runs glues Download PDFInfo
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- CN205175959U CN205175959U CN201520862470.9U CN201520862470U CN205175959U CN 205175959 U CN205175959 U CN 205175959U CN 201520862470 U CN201520862470 U CN 201520862470U CN 205175959 U CN205175959 U CN 205175959U
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- glue
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Abstract
The utility model discloses a many V -arrangements pipe is an appearance ware upward for albumen runs glues, a serial communication port, include a plurality of V -arrangement pipes of forming a complete set with SDS -PAGE film pectination tooth's socket quantity, by horizontal connecting leg intercommunication, all be equipped with one at the bottom of the V -arrangement of every V -arrangement pipe and annotate a kind hole between this a plurality of V -arrangements pipe, locate all to be equipped with an application of sample mouth with horizontal connecting leg intercommunication on one side of the V -arrangement opening of every V -arrangement pipe, during the application of sample, aim at the corresponding pectination tooth's socket of SDS -PAGE film respectively with each V -arrangement pipe, into fixed a position in a pectination tooth's socket all stretching at the bottom of every V -arrangement pipe V -arrangement.
Description
Technical field
The utility model relates to a kind of molecular biology experiment technology, and particularly a kind of SDS-PAGE (polyacrylamide gel electrophoresis) runs the application of sample apparatus of protein sample in glue operating process.
Background technology
In molecular biology experiment technical field, modal is Westernblot experiment (western blot test), and whole experimentation comprises---manufacture SDS-PAGE film, loading, electrophoresis, transferring film, close, add antibody, luminescence etc.In glue loading process, the concentration of glue depends on destination protein molecular weight analyte size, and the size of applied sample amount depends on that the speed that the result of protein quantification, sample protein are run is by the pH value of electrolytic solution, and voltage and gravity determine jointly.When the specific electrolytic solution of use, and when maintaining relative constancy voltage, the speed that sample protein declines depends primarily on size and the weight (namely the larger race of molecular weight is slower) of protein molecular, the order of accuarcy of applied sample amount directly affects electrophoretic effects, therefore, glue and loading are most important in electrophoresis process.Current glue technology is mature technology, and loading process adopts sample loading gun that sample one lattice one lattice of different albumen are expelled to (feeding chamber in Fig. 1) in the teeth groove of pectination SDS-PAGE film 4.But, often there is following problem in use: 1. rifle head or syringe needle easily puncture gel, directly affect quality of experiments; 2. application of sample can make sample go out feeding chamber 2 too soon, if there is bubble that sample also may be made to overflow; 3. easily mix mutually between different sample between different feeding chamber, cannot cross pollution be avoided.Unnecessary error is caused to Coupon Testing Results.
Summary of the invention
The purpose of this utility model is to provide that a kind of structure is simple, loading is accurate, convenient and time-saving, substantially can meet the race glue application of sample utensil that Westernblot tests various protein sample demand.
For reaching above object, the utility model takes following technical scheme to be achieved:
Run many V-arrangements pipe injector of glue for albumen, it is characterized in that, comprise the multiple V-arrangement pipes supporting with SDS-PAGE film pectination teeth groove quantity, be communicated with by horizontal connecting leg between the plurality of V-arrangement pipe, at the bottom of the V-arrangement of each V-arrangement pipe, be equipped with a note sample hole; V-arrangement opening side and the horizontal connecting leg connectivity part of each V-arrangement pipe are equipped with an adding mouth, during application of sample, each V-arrangement pipe are aimed at respectively the corresponding pectination teeth groove of SDS-PAGE film, all will to put in a pectination teeth groove and located at the bottom of each V-arrangement pipe V-arrangement.
In such scheme, in described multiple V-arrangement pipe, high order end is connected a ram's horn respectively with the V-arrangement pipe of low order end, these two symmetrical distributions of ram's horn, when two ram's horns are symmetrical be fixed on run on glue sampling device left and right sides column time, by all to put at the bottom of each V-arrangement pipe V-arrangement in a pectination teeth groove and to be located.
Described V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.
Described adding mouth is a funnel.This funnel can dock with 10 microlitre sample loading gun heads.
Compared with prior art, the utility model conveniently moving, accurate positioning, rational in infrastructure, and, facilitate, save time, economy, science, meet the demand of current various race glue loading.
Accompanying drawing explanation
Fig. 1 is SDS-PAGE film structure figure in existing race glue sampling device.
Fig. 2 is the utility model erietiform injector structural representation.
Fig. 3 is I enlarged drawing in Fig. 2.
Fig. 4 is the schematic diagram of SDS-PAGE film pectination teeth groove Fig. 2 injector being placed in Fig. 1 device.
In Fig. 1 ~ Fig. 4: 1, electrode terminal; 2, feeding chamber; 3, rear plate plane; 4, SDS-PAGE film; 5, front plate plane; 6, column; 7, horizontal connecting leg; 8, V-arrangement pipe; 9, adding mouth; 10, ram's horn; 11, funnel; 12, sample hole is noted.
Embodiment
With reference to figure 1, in existing race glue sampling device, adopt forward and backward clamping plate (transparent glass sheet, not shown in FIG.) in the gap that it is parallel, first prepare SDS-PAGE film 4, insert the mould of a pectination in film in forward and backward clamping plate upper end before solidifying, after film solidifies, extract pectination mould, thus make to form multiple teeth groove between film upper end and forward and backward clamping plate plane 5,3, be also feeding chamber 2.Existing loading methods adopts sample loading gun (entry needle) to be expelled in different feeding chambers respectively by the sample of different albumen, as described in background technology, there is many defects.
With reference to figure 2, Fig. 4, the utility model devises a kind of many V-arrangements pipe injector, comprise the multiple V-arrangement pipes 8 supporting with SDS-PAGE film 4 teeth groove, be communicated with by horizontal connecting leg 7 between multiple V-arrangement pipe, each V-arrangement pipe V-arrangement opening side and horizontal connecting leg connectivity part are equipped with an adding mouth 9, V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.In the structure of multiple V-arrangement pipe 8 be communicated with by horizontal connecting leg, the V-arrangement pipe of high order end connects a ram's horn 10, and the V-arrangement pipe of low order end connects another ram's horn 10, these two symmetrical distributions of ram's horn.During application of sample, each V-arrangement pipe of Fig. 2 erietiform injector is aimed at respectively the corresponding pectination teeth groove (Fig. 4) of SDS-PAGE film 4, two ram's horns are hung on the upper end that Fig. 1 runs the corresponding column 6 of glue sampling device respectively, thus make each V-arrangement Guan Jun of injector stretch into a feeding chamber 2 and be located.
With reference to figure 3, adding mouth 9 is a funnel 11, and this funnel can dock with 10 microlitre sample loading gun heads, and when can avoid application of sample, sample leaks outside.Be equipped with a note sample hole 12 at the bottom of each V-arrangement pipe V-arrangement, sample can accurately add in corresponding feeding chamber (track) by it, overcomes the maloperation that in the past clearly cannot distinguish swimming lane.
This note sample hole is also communicated with the adding mouth of adjacent V-arrangement pipe outside being communicated with the adding mouth 9 of this V-arrangement pipe, like this, can prevent back flow of sample on the one hand, the bubble added in feeding chamber sample can be discharged on the other hand, prevent the pollution to adjacent feeding chamber sample.
Claims (4)
1. run many V-arrangements pipe injector of glue for albumen, it is characterized in that, comprise the multiple V-arrangement pipes supporting with SDS-PAGE film pectination teeth groove quantity, be communicated with by horizontal connecting leg between the plurality of V-arrangement pipe, at the bottom of the V-arrangement of each V-arrangement pipe, be equipped with a note sample hole; V-arrangement opening side and the horizontal connecting leg connectivity part of each V-arrangement pipe are equipped with an adding mouth, during application of sample, each V-arrangement pipe are aimed at respectively the corresponding pectination teeth groove of SDS-PAGE film, all will to put in a pectination teeth groove and located at the bottom of each V-arrangement pipe V-arrangement.
2. many V-arrangements pipe injector of glue is run as claimed in claim 1 for albumen, it is characterized in that, in described multiple V-arrangement pipe, high order end is connected a ram's horn respectively with the V-arrangement pipe of low order end, these two symmetrical distributions of ram's horn, when two ram's horns are symmetrical be fixed on run on glue sampling device left and right sides column time, by all to put at the bottom of each V-arrangement pipe V-arrangement in a pectination teeth groove and to be located.
3. run many V-arrangements pipe injector of glue as claimed in claim 1 for albumen, it is characterized in that, described V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.
4. run many V-arrangements pipe injector of glue as claimed in claim 1 for albumen, it is characterized in that, described adding mouth is a funnel, and this funnel can dock with 10 microlitre sample loading gun heads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520862470.9U CN205175959U (en) | 2015-11-02 | 2015-11-02 | A many V -arrangements pipe is an appearance ware upward for albumen runs glues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520862470.9U CN205175959U (en) | 2015-11-02 | 2015-11-02 | A many V -arrangements pipe is an appearance ware upward for albumen runs glues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205175959U true CN205175959U (en) | 2016-04-20 |
Family
ID=55739935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520862470.9U Expired - Fee Related CN205175959U (en) | 2015-11-02 | 2015-11-02 | A many V -arrangements pipe is an appearance ware upward for albumen runs glues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205175959U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259241A (en) * | 2015-11-02 | 2016-01-20 | 中国人民解放军第四军医大学 | Multi-V-shaped pipe sample applicator for protein running gel |
-
2015
- 2015-11-02 CN CN201520862470.9U patent/CN205175959U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259241A (en) * | 2015-11-02 | 2016-01-20 | 中国人民解放军第四军医大学 | Multi-V-shaped pipe sample applicator for protein running gel |
| CN105259241B (en) * | 2015-11-02 | 2018-01-30 | 中国人民解放军第四军医大学 | A kind of more V-arrangement pipe injectors that glue is run for albumen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20161102 |