CN205080086U - Detection apparatus for glycated haemoglobin - Google Patents
Detection apparatus for glycated haemoglobin Download PDFInfo
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- CN205080086U CN205080086U CN201520778385.4U CN201520778385U CN205080086U CN 205080086 U CN205080086 U CN 205080086U CN 201520778385 U CN201520778385 U CN 201520778385U CN 205080086 U CN205080086 U CN 205080086U
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Abstract
The utility model relates to a medical science detection area, in particular to detection apparatus for glycated haemoglobin. Including the conversion zone, the conversion zone includes casing, home sensor, reaction zone and application of sample district, home sensor includes electrode and flow path, the flow path is provided with the electron transfer material, the reaction zone is provided with boron acid derivatives. The utility model provides a detection device, as long as pour into 20 microliter blood into, alright detect out glycated haemoglobin's concentration with simple detection method, still can improve the shortcoming among the glycated haemoglobin detection mode who goes on with the standard practices. Detect the glycated haemoglobin in the blood, the utility model discloses not only non professional convenient to use does not need dear equipment yet, inspects moreover to have the accuracy.
Description
Technical field
The utility model relates to medical science, particularly the pick-up unit of glycosylated hemoglobin.
Background technology
Diagnose and prevent diabetes, periodically should detect the glucose amount in blood.This blood sugar test can use portable detector, and detection method is simple and convenient.But blood sugar changes along with the health of patient, altering a great deal of its value.Glycosylated hemoglobin is that the glucose process in blood reacts the texturising protein generated for a long time.As everyone knows, the mean value of this glycosylated hemoglobin and 2 ~ 3 months blood sugar amounts has close relation.Also taking measures in time if daily blood sugar test is the health status in order to understand every day, so detecting glycosylated hemoglobin then in order to grasp the medium-term and long-term Blood sugar management state of patient to take counter-measure.
It is utilize HPLC gathered blood sample is carried out separation and optically detects that the glycosylated hemoglobin of standard detects.The shortcoming of the standard detecting method of this glycosylated hemoglobin is the equipment that needs are expensive.Moreover, a difficult problem is also had to be to ask professional to use by correct method of operating.In order to address these problems, need further research.
In biology sensor, electrochemical means is one of analytical approach be widely used.During special component in analyzing blood, apply specific voltage, then by redox reaction, utilize the flowing of electronics to carry out a kind of method analyzed.
Glycosylated hemoglobin and boronic acid derivatives can carry out special combination, and this point is known by people.Apply the singularity of this reaction, boronic acid derivatives connects chromophore, then adopt optical means to detect.But need the washing process carrying out unreacted boronic acid derivatives, but also there is a difficult point, all will can draw accurate result by collecting sample capacity exactly exactly at every turn.
Therefore, the pick-up unit of good, the easy and simple to handle glycosylated hemoglobin of a kind of accuracy is provided to have important practical significance.
Utility model content
In view of this, the utility model provides a kind of pick-up unit of glycosylated hemoglobin.The pick-up unit accuracy of this glycosylated hemoglobin is good, easy and simple to handle.
In order to realize above-mentioned utility model object, the utility model provides following technical scheme:
The utility model provides a kind of pick-up unit of glycosylated hemoglobin, comprises responding layer 1;
Described responding layer comprises housing 2, internal sensor 3, reaction zone 4 and sample application zone 7;
Described internal sensor 3 comprises electrode 5 and stream 6;
Described stream 6 is provided with electron transport substance;
Described reaction zone 4 is provided with boronic acid derivatives.
In specific embodiments more of the present utility model, described electrode 5 is gold electrode.
In specific embodiments more of the present utility model, described electrode 5 is at least 2.
In specific embodiments more of the present utility model, also comprise application of sample layer 8, described application of sample layer 8 is provided with well 11, and described well 11 is equipped with described sample application zone 7.
In specific embodiments more of the present utility model, also comprise articulamentum 9, described application of sample layer 8 is connected by described articulamentum 9 with described responding layer 1.
In specific embodiments more of the present utility model, described articulamentum 9 is provided with air vent 10.
In specific embodiments more of the present utility model, the structure of described boronic acid derivatives is such as formula shown in I
Formula I
Wherein, R is that at least 10 linear carbon elements connect.
In specific embodiments more of the present utility model, the material of described housing 2 is plastics or silica gel.
In specific embodiments more of the present utility model, described internal sensor 3 is arranged at described housing 2 by being installed in addition with, engaging or etch.
In specific embodiments more of the present utility model, the material of described articulamentum 9 is plastics or glass; The material of the reaction zone 4 of described responding layer 1 is glass fibre or porous polymeric material.
The utility model gives self-assembled film function to boronic acid derivatives, thus detects simply with electrochemical means.If give the self-assembled film function of suitable length to boronic acid derivatives, will react with glycosylated hemoglobin, unreacted boronic acid derivatives then oneself forms film on gold electrode.The film that gold electrode is formed is formed more, more unfavorable to the redox reaction of electron transport substance under same voltage.Utilize this characteristic, the concentration of the glycosylated hemoglobin of the current value that back titration mode produces can be calculated.
The pick-up unit that the utility model provides, as long as inject 20 microliters of blood, just can detect the concentration of glycosylated hemoglobin by simple detection method, also can improve the shortcoming in the glycosylated hemoglobin detection mode of carrying out with standard method.
Detect the glycosylated hemoglobin in blood, the utility model not only layman is easy to use, does not also need expensive equipment, and inspection has accuracy.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 shows the principle of the pick-up unit generation electrochemical reaction that the utility model provides; Wherein Fig. 1 (A) shows principle of electrochemical reaction; Fig. 1 (B) shows pictorial diagram; Wherein, 1-A shows electron transport substance; 1-B shows glycosylated hemoglobin; 1-C shows haemoglobin; 1-D shows boronic acid derivatives;
Fig. 2 shows the structural representation of the utility model responding layer; Wherein, 1-responding layer, 2-housing, 3-internal sensor, 4-reaction zone, 5-gold electrode (sun is carved), 6-stream (cloudy quarter), 7-reaction zone, 2-A shows internal sensor tank (the moon is carved);
Fig. 3 shows the structural representation of the utility model application of sample layer; Wherein 11-well;
Fig. 4 shows the structural representation of the utility model articulamentum; Wherein 10-air vent;
Structural representation after show Fig. 5 the utility model articulamentum and responding layer combine; Wherein, 4-reaction zone, is provided with borate biological; 6-stream, because of the combination of articulamentum and responding layer, forms the stream producing capillarity; Stream is provided with electron transport substance; 10-air vent;
Fig. 6 shows the structural representation of the utility model pick-up unit; Wherein 8-application of sample layer; 9-articulamentum; 1-responding layer;
Fig. 7 shows the schematic diagram of the utility model pick-up unit; Wherein, 11-well;
Fig. 8 shows the reacting phenomenon of the utility model pick-up unit; Wherein, Fig. 8-A shows that electron transport substance (Mediator) smears position; Fig. 8-B shows that boronic acid derivatives smears position; Fig. 8-C shows injection blood; Fig. 8-D shows filtration position absorbing blood, boronic acid derivatives and glycosylated hemoglobin reaction; Fig. 8-E shows that the boronic acid derivatives do not reacted with glycosylated hemoglobin flow to stream because of capillary phenomenon, moves together with electron transport substance to electrode; Fig. 8-F shows that the borate biological do not reacted with glycosylated hemoglobin forms film on gold electrode; Fig. 8-G is shown on oxygen electrode and applies specific permission voltage, and detect produce the magnitude of current, thus detect the concentration of glycosylated hemoglobin; After well 11 injects blood, reaction zone 4 absorbing blood, boronic acid derivatives and glycosylated hemoglobin reaction, the boronic acid derivatives do not reacted with glycosylated hemoglobin flow to stream 6 because of capillarity, moves together with electron transport substance to electrode 5; The boronic acid derivatives do not reacted with glycosylated hemoglobin forms film on electrode 5; Oxygen electrode applies specific permission voltage, and detects the magnitude of current produced, thus detect the concentration of glycosylated hemoglobin;
Fig. 9 shows the test result of embodiment 2, the relation of electric current and glycosylated hemoglobin; Wherein, Fig. 9 (A) shows that the concentration of glycosylated hemoglobin is 4%; Fig. 9 (B) shows that the concentration of glycosylated hemoglobin is 8%; Fig. 9 (C) shows that the concentration of glycosylated hemoglobin is 12%;
Figure 10 shows the typical curve that embodiment 1 is obtained.
Embodiment
The utility model discloses a kind of pick-up unit of glycosylated hemoglobin, those skilled in the art can use for reference present disclosure, and suitable improving technique parameter realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included in the utility model.Method of the present utility model and application are described by preferred embodiment, related personnel obviously can not depart from the utility model content, spirit and scope methods and applications as herein described are changed or suitably change with combination, realize and apply the utility model technology.
The pick-up unit of glycosylated hemoglobin, comprises responding layer 1;
Described responding layer comprises housing 2, internal sensor 3, reaction zone 4 and sample application zone 7;
Described internal sensor 3 comprises electrode 5 and stream 6;
Described stream 6 is provided with electron transport substance;
Described reaction zone 4 is provided with boronic acid derivatives.
In specific embodiments more of the present utility model, described electrode 5 is gold electrode.
In specific embodiments more of the present utility model, described electrode 5 is at least 2.
In specific embodiments more of the present utility model, also comprise application of sample layer 8, described application of sample layer 8 is provided with well 11, and described well 11 is equipped with described sample application zone 7.
In specific embodiments more of the present utility model, also comprise articulamentum 9, described application of sample layer 8 is connected by described articulamentum 9 with described responding layer 1.Wherein, 4-reaction zone, is provided with borate biological; 6-stream, because of the combination of articulamentum and responding layer, forms the stream producing capillarity; Stream is provided with electron transport substance.
In specific embodiments more of the present utility model, described articulamentum 9 is provided with air vent 10.
In specific embodiments more of the present utility model, the structure of described boronic acid derivatives is as follows
Wherein, R is that at least 10 linear carbon elements connect.
In specific embodiments more of the present utility model, the material of described housing 2 is plastics or silica gel.
In specific embodiments more of the present utility model, described internal sensor 3 is arranged at described housing 2 by being installed in addition with, engaging or etch.
In other specific embodiments of the present utility model, the material of described articulamentum 9 is plastics or glass; The material of the reaction zone 4 of described responding layer 1 is glass fibre or porous polymeric material.
After well 11 injects blood, reaction zone 4 absorbing blood, boronic acid derivatives and glycosylated hemoglobin reaction, the boronic acid derivatives do not reacted with glycosylated hemoglobin flow to stream 6 because of capillarity, moves together with electron transport substance to electrode 5; The boronic acid derivatives do not reacted with glycosylated hemoglobin forms film on electrode 5; Oxygen electrode applies specific permission voltage, and detects the magnitude of current produced, thus detect the concentration of glycosylated hemoglobin.
In the pick-up unit of the glycosylated hemoglobin that the utility model provides, element used and starting material all can be buied by market.
Below in conjunction with embodiment, set forth the utility model further:
The acquisition of embodiment 1 typical curve
The making of typical curve:
Configure the blood sample that glycosylated hemoglobin concentration is 4%, 8%, 12% respectively, be labeled as sample 1, sample 2, sample 3; Through the pick-up unit that the utility model provides, measurement result is in table 1, Fig. 9:
Table 1 testing result
| Group | Electric current (uA) | Saccharification hemoglobin content (%) |
| Sample 1 | 100 | 4.0 |
| Sample 2 | 330 | 8.0 |
| Sample 3 | 610 | 12.0 |
Obtain the relation of electric current and saccharification hemoglobin content:
If electric current is y, saccharification hemoglobin content is x;
Then y=63.75x-163.33(R
2=0.9968)
Typical curve is shown in Figure 10.
The feasibility detection experiment of embodiment 2 pick-up unit
Configuration glycosylated hemoglobin concentration is the blood sample of 3.0%, 6.0%, 9.0%, is labeled as sample 1, sample 2, sample 3; Through the pick-up unit that the utility model provides, obtain corresponding current value, and obtain the content of glycosylated hemoglobin by the typical curve of embodiment 1, in table 2:
Table 2 testing result
| Group | Electric current (uA) | Saccharification hemoglobin content (%) |
| Sample 1 | 30 | 3.0 |
| Sample 2 | 225 | 6.0 |
| Sample 3 | 405 | 8.9 |
As seen from the results in Table 2, the glycated protein content of the sample 1 ~ 3 that the pick-up unit provided through the utility model obtains is respectively 3.0%, 6.0%, 8.9%, with the concentration configured without significant difference (P > 0.05), therefore, the pick-up unit that the utility model provides has feasibility.
Embodiment 3 detection experiment
Configuration glycosylated hemoglobin concentration to be measured is the blood sample of 4.0%, 7.5%, 11.0%, is labeled as sample 1, sample 2, sample 3;
Experimental group: the pick-up unit provided through the utility model, obtains corresponding current value, and obtains the content of glycosylated hemoglobin by the typical curve of embodiment 1;
Control group 1: detect above-mentioned sample to be tested with the existing pick-up unit in this area;
The results are shown in Table 3:
Table 3 testing result
As seen from the results in Table 3, the glycated protein content of the sample 1 ~ 3 that the pick-up unit provided through the utility model obtains is respectively 3.9%, 7.4%, 11.1%, with the concentration configured without significant difference (P > 0.05), therefore, the pick-up unit that the utility model provides has feasibility.
The glycated protein content of the sample 1 ~ 3 obtained through the pick-up unit of control group 1 is respectively 4.1%, 7.4%, 10.9%, and there were significant differences (P < 0.05) with the concentration of configuration, and therefore, the pick-up unit of control group 1 is inaccurate.
In addition, compared with control group 1, control group 2, the pick-up unit pole that the utility model provides significantly shorten detection time (P < 0.01), and pole significantly reduces testing cost (P < 0.01).
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (10)
1. a pick-up unit for glycosylated hemoglobin, is characterized in that, comprises responding layer (1);
Described responding layer comprises housing (2), internal sensor (3), reaction zone (4) and sample application zone (7);
Described internal sensor (3) comprises electrode (5) and stream (6);
Described stream (6) is provided with electron transport substance;
Described reaction zone (4) is provided with boronic acid derivatives.
2. pick-up unit according to claim 1, is characterized in that, described electrode (5) is gold electrode.
3. pick-up unit according to claim 1, is characterized in that, described electrode (5) is at least 2.
4. pick-up unit according to claim 1, it is characterized in that, also comprise application of sample layer (8), described application of sample layer (8) is provided with well (11), and described well (11) and described sample application zone (7) are equipped with.
5. pick-up unit according to claim 4, is characterized in that, also comprises articulamentum (9), and described application of sample layer (8) is connected by described articulamentum (9) with described responding layer (1).
6. pick-up unit according to claim 5, is characterized in that, described articulamentum (9) is provided with air vent (10).
7. pick-up unit according to claim 1, is characterized in that, the structure of described boronic acid derivatives is as follows
Wherein, R is that at least 10 linear carbon elements connect.
8. pick-up unit according to claim 1, is characterized in that, the material of described housing (2) is plastics or silica gel.
9. pick-up unit according to claim 1, is characterized in that, described internal sensor (3) is arranged at described housing (2) by being installed in addition with, engaging or etch.
10. pick-up unit according to claim 5, is characterized in that, the material of described articulamentum (9) is plastics or glass; The material of the reaction zone (4) of described responding layer (1) is glass fibre or porous polymeric material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520778385.4U CN205080086U (en) | 2015-10-09 | 2015-10-09 | Detection apparatus for glycated haemoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520778385.4U CN205080086U (en) | 2015-10-09 | 2015-10-09 | Detection apparatus for glycated haemoglobin |
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| Publication Number | Publication Date |
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| CN205080086U true CN205080086U (en) | 2016-03-09 |
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| CN201520778385.4U Active CN205080086U (en) | 2015-10-09 | 2015-10-09 | Detection apparatus for glycated haemoglobin |
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