CN205062070U - Culture dish calibration device and culture dish - Google Patents

Abstract

The utility model relates to a medical experiment utensil field particularly, relates to a culture dish calibration device and culture dish. Include: mark the main part, it is used for the connecting elements that is connected with the culture dish to mark to be provided with in the main part, it marks the component still to be provided with the position in the demarcation main part. It can connect through connecting elements and culture dish detachable to mark the main part. Generally, because the structure of culture dish generally includes end box and lid, and consequently regular the opening of lid meeting is markd main part end box detachable general and culture dish and is connected. The component is markd to this position can be the transparent just in the outside optional position of maring the culture dish of end box. And when doing the experiment, the experimenter accessible position is markd the component and is accurately fixed a position the target area in the culture dish, be convenient for to this regional target go on accuracy amazing or in succession developments observe, and then reduce the errors because of stimulation or observation ward territory difference at every turn arouses to can obtain more accurate experimental result.

Description

Culture dish calibration device and culture dish

Technical Field

The utility model relates to a medical science experiment utensil field particularly, relates to a culture dish calibration device and culture dish.

Background

At present, in various medical experiments, a culture dish is often used to culture microorganisms or cells. Generally, after cells are inoculated into a culture dish, the cell density at different positions of the culture dish is difficult to ensure to be completely consistent due to various reasons such as non-directional flowing of culture solution or uneven plane on which the culture dish is placed, and the phenomenon is particularly obvious when the cells grow adherently before the cells adhere to the culture dish. When experimenters need to dynamically observe cell or microorganism forms, the experimenters randomly observe a plurality of visual fields each time to generally judge the cell forms or cell densities, and cannot ensure that the observation of each time is the target of the same area, so that the accurate dynamic observation of the target of a certain determined area is difficult to realize.

In the scratch test, for example, it is necessary to scribe a plurality of scratches on a culture dish to verify the proliferation and migration of cells. Adopt traditional cell culture dish, when carrying out dynamic observation to mar department cell, the local of a mar can only be observed in a field of vision, is difficult to fix a position the specific position of cell in this mar in the field of vision now, and when microscope magnification increased, this kind of phenomenon was more obvious. Thus, it is difficult to ensure that cells at the same position of the same scratch are observed at each time, and different observation results may lack comparability and continuity depending on the target position of observation.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model aims at providing a culture dish calibration device and culture dish can more accurately mark arbitrary position in the culture dish, and the experimenter of being convenient for carries out the developments to certain definite target area in follow-up experiment and observes, is difficult to appear great deviation to can acquire more accurate experimental result.

In a first aspect, an embodiment of the present invention provides a culture dish calibration apparatus, wherein, include: calibrating a main body;

the calibration main body is provided with a connecting component used for being connected with a culture dish;

the calibration main body is also provided with a position calibration component.

In combination with the first aspect, the present invention provides a first possible implementation manner of the first aspect, wherein,

the position calibration means comprises: the rotating disc is rotatably connected with the calibration main body;

and a position calibration hole is formed in the rotating disc.

In combination with the first aspect, the present invention provides a second possible implementation manner of the first aspect, wherein,

the calibration main body is further provided with a clamping groove for mounting the position calibration component. In combination with the first aspect, the present invention provides a third possible implementation manner of the first aspect, wherein,

the position calibration hole is at least one, and the position calibration hole is at least one of a circular hole, a polygonal hole and a long-strip-shaped hole.

In combination with the first aspect, the present invention provides a fourth possible implementation manner of the first aspect, wherein,

the rotating disc is circular or sector;

when the rotating discs are in a fan shape, at least one fan-shaped rotating disc is arranged, and the rotating axes of all the rotating discs are overlapped;

the calibration main body is further provided with a rotating shaft which is rotatably connected with the fan-shaped rotating disc.

In combination with the first aspect, the present invention provides a fifth possible implementation manner of the first aspect, wherein,

the rotating disc is also provided with a rotating bulge.

In combination with the first aspect, the present invention provides a sixth possible implementation manner of the first aspect, wherein,

the sliding rail or the sliding groove is arranged on the calibration main body;

the sliding rail or the sliding chute is also connected with a calibration ruler in a sliding way;

the calibration ruler is also provided with a calibration clamping piece which can slide along the calibration ruler.

In combination with the first aspect, the present invention provides a seventh possible implementation manner of the first aspect, wherein,

the calibration clamping piece is also provided with an observation through hole.

In combination with the first aspect, the present invention provides an eighth possible implementation manner of the first aspect, wherein,

the connection member includes: mounting grooves;

the side wall of the mounting groove is also provided with an anti-skid component;

or,

the connection member includes: a clamping mechanism.

In a second aspect, embodiments of the present invention further provide a culture dish, wherein,

the method comprises the following steps: the box comprises a bottom box and a cover covering the opening of the bottom box;

the culture dish calibration device is characterized by further comprising the culture dish calibration device according to the first aspect;

the culture dish calibration device is detachably connected with the bottom box.

The embodiment of the utility model provides a culture dish calibration device, it is connected that the main part is can be through connecting elements and culture dish detachable to mark. Generally, the calibration body is removably connected to the bottom box of the culture dish, since the structure of the culture dish generally comprises a bottom box and a lid, which are frequently opened. The position calibration component is any position where the culture dish can be calibrated outside the bottom box and is transparent. When carrying out cell culture related experiments, experimenters can accurately position a target area in a culture dish through a position calibration component, so that the experimenters can accurately stimulate cells or microorganisms in a certain fixed area (such as cell scratch experiments), and can also dynamically observe the forms of the cells or the microorganisms in the fixed area. The error caused by different stimulation or observation areas can be reduced, so that more accurate experimental results can be obtained.

In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a front view of a culture dish according to an embodiment of the present invention;

FIG. 2 is a schematic view of a rotating disc of a culture dish according to an embodiment of the present invention;

FIG. 3 is a schematic front view of another culture dish according to an embodiment of the present invention;

FIG. 4 is a schematic view of a rotating disc of another culture dish according to an embodiment of the present invention;

FIG. 5 is a schematic structural diagram of a third culture dish according to an embodiment of the present invention;

FIG. 6 is a schematic bottom view of a third culture dish according to an embodiment of the present invention;

fig. 7 is a schematic front view of a culture dish according to an embodiment of the present invention.

Illustration of the drawings:

10-calibrating the main body; 20-a connecting member; 30-position calibration means; 40-a rotating disc; 50-position calibration holes; 60-a clamping groove; 70-a rotating shaft; 80-rotating the projection; 90-scaling; 100-calibrating a clamping piece; 110-slide or slide way; 120-viewing through holes; 130-a mounting groove; 140-a slip-resistant member; 150-bottom box; 160-a lid; 170-a limit table; 180-positioning member.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiment of the present invention, all other embodiments obtained by the person skilled in the art without creative work belong to the protection scope of the present invention.

The culture dish used at present generally comprises a bottom box and a cover which is covered on the bottom box due to the structure, and the bottom box and the cover are two separated individuals, because neither the bottom box nor the cover has a reference mark, if a certain area in the culture dish needs to be dynamically observed for a plurality of times during the experiment, it is easy to make the position of each observation inconsistent because of no reliable reference, which may cause relatively large errors in the final observation result, and therefore, the present application provides a culture dish, can accurately calibrate the target observation position according to the needs of experimenters, is not easy to generate large position deviation, therefore, errors of observation results caused by different observation positions are reduced, and accuracy, continuity and comparability of different observation results can be guaranteed.

For the convenience of understanding this embodiment, the following is to the culture dish calibration component disclosed in the embodiments of the present invention and is described in detail, refer to fig. 1, the present invention provides a culture dish including: calibrating the main body 10;

the calibration main body 10 is provided with a connecting component 20 for connecting with a culture dish;

the calibration main body 10 is further provided with a position calibration component 30;

the connecting component is also provided with a positioning component used for determining the unique connecting position of the culture dish.

When implemented, the calibration body 10 is detachably connected to the culture dish via the connecting member 20. Generally, the calibration body 10 is removably connected to the bottom box of the culture dish, since the structure of the culture dish generally comprises a bottom box and a lid, which is frequently opened. The position calibration component is any position where the culture dish can be calibrated outside the bottom box and is transparent. When the experiment is carried out, experimenters can accurately mark any position needing to be observed in the culture dish through the calibration component according to research needs, and errors caused by stimulation or different observation areas at each time can be reduced during subsequent observation, so that more accurate experiment results can be obtained.

When the positioning member 180 is a concave hole, a protrusion or the like, the protrusion is arranged on the calibration main body 10, when the positioning member 180 is a concave hole, the culture dish is provided with a protrusion which is matched with the concave hole, the concave hole can be a groove-shaped hole or a common concave hole, and the protrusion arranged on the culture dish can slide into the groove-shaped hole or can be directly inserted into the common concave hole; when the positioning member 180 is a protrusion, a concave hole matched with the protrusion is also arranged on the culture dish. In addition, the positioning member 180 may also be a sticker, a scratch made with a colored pen, or the like.

Referring to fig. 1 and fig. 2, a structure of a position calibration member 30 provided in an embodiment of the present invention specifically includes:

a rotating disc 40 rotatably connected with the calibration body 10;

the rotating disc 40 is provided with a position calibration hole 50.

In a practical implementation, the diameter of the rotating disc 40 is equal to or slightly larger than the diameter of the bottom box of the culture dish. Therefore, the connection between the calibration body 10 and the culture dish is larger than the surface of the bottom case connected with the calibration body 10. And the rotary disk 40 is rotatably connected with the calibration body 10 such that the position calibration hole 50 provided on the rotary disk 40 can move on a circle having a center of rotation of the rotary disk and a radius of a distance between the center of the position calibration hole 50 and the center of the rotary disk 40 as a center of rotation of the rotary disk 40. In general, there are a plurality of position calibration holes 50, and the plurality of position calibration holes 50 are regularly or irregularly arranged on the rotary disk 40.

It should be noted, however, that no matter how the position calibration holes 50 are arranged, when the position calibration holes 50 change their relative positions with respect to the bottom case as the rotating disc 40 rotates, at least one position calibration hole 50 can cover any position in the bottom case when rotating.

Preferably, the rotating disk 40 and the bottom surface of the bottom case should be parallel when the bottom case is mounted on the calibration body 10. The axis of rotation of the rotatable disk 40 should also be perpendicular to the bottom surface of the back box.

Generally, the size or shape of the observation area for a specific position in the culture dish is different according to the experiment, so the position calibration holes 50 can be circular holes, polygonal holes or strip-shaped holes.

In a specific implementation, the rotating disc 40 disposed on the same culture dish may have one shape of the position calibration hole, or may have a plurality of shapes of the position calibration holes 50. Meanwhile, even if the shape of the position calibration holes 50 is the same on the same rotating disk 40, a plurality of groups of position calibration holes 50 having different hole diameters may be provided. For example, fig. 2 shows an embodiment of the present invention, a plurality of sets of circular holes are provided, and the apertures of the plurality of sets of circular holes are the same, so that the position calibration hole 50 meeting the experimental requirements can be selected to calibrate the position to be observed according to the actual requirements. In addition, a plurality of groups of circular holes can be set to have different apertures, so that the position calibration holes 50 with different apertures can be selected according to the specific observation requirements of the experiment for calibration.

In addition, referring to fig. 3, in order to enable the rotary disk 40 to be rotatably connected with the calibration body 10, a clamping groove 60 for mounting the position calibration member is further provided on the calibration body 10.

In the specific implementation, since the shape of the upper bottom box of the culture dish is generally oblate, in order to ensure that the position calibration holes 50 on the rotary disk 40 can calibrate different positions on different culture dishes, the shape of the rotary disk 40 is generally circular. When the rotary disk 40 is circular in shape, the slot 60 is generally in the shape of a circular ring disposed on the calibration body 10. To ensure that the diameter of the rotating disk 40 is equal to or slightly larger than the diameter of the bottom plate, the diameter of the slot 60 is generally larger than the diameter of the bottom plate. The rotary disk 40 can be just caught in the catch 60 therein, so that the rotary disk 40 can rotate in the catch 60.

In addition, referring to FIGS. 3 and 4, the rotating disk 40 may be configured as a sector, taking into account that there may be more than one location in the same culture dish that needs to be calibrated at some time. And a plurality of fan-shaped rotating disks 40 are mounted on the same culture dish. Generally, the axes of rotation of all the fan-shaped rotary disks 40 are coincident. Thus, different positions of the same culture dish can be calibrated by adjusting the positions of different sectors. It should be noted that all of the fan-shaped rotating disks 40 may be on the same plane, so that the rotating disks 40 cannot be overlapped while rotating; thus, only one locking groove 60 is required, and all the rotating discs 60 are arranged in the locking groove 60. The segments of the rotatable disk 40 may be staggered so that different rotatable disks 40 may overlap when rotated, and the number of slots 60 should be equal to the number of rotatable disks 40. In addition, the two arrangements can be combined, for example, the rotating discs 40 are divided into a plurality of groups, each group has at least two rotating discs 40, each group of rotating discs 40 is in the same plane, and are also installed in the same slot 60. While different sets of discs 40 are staggered and mounted in different pockets 60.

It should be noted that when the rotating disc 40 is a sector, a rotating shaft 70 rotatably connected to the rotating disc 40 is further provided on the calibration body 10. All the sector-shaped rotary disks 40 are rotatably connected to the rotary shaft 70.

In addition, referring to fig. 2, in order to conveniently control the rotation of the rotary disk 40, a rotary protrusion 80 is further provided on the rotary disk 40. When performing position calibration, the operator can push the rotating protrusion 80 with his finger to rotate the rotating disk 40 on the calibration body 10.

The rotating protrusion 80 may be one, and may be disposed at a position close to the edge of the rotating disk 40, or two or more, and may be disposed at two sides of the rotating disk 40 close to the edge. In fig. 2, only the arrangement when there are two rotating protrusions 80 is shown, and the two rotating protrusions 80 are respectively provided at both ends of the same diameter of the rotating disk 40.

It should be noted that the arrangement mode in fig. 2 is the most labor-saving arrangement mode, the rotating protrusion 80 may be disposed at other positions, the embodiment shown in fig. 2 is only one of them, and other arrangement modes of the rotating protrusion 80 should also be within the protection scope of the present invention.

Referring to fig. 5 and 6, the embodiment of the present invention further provides another structure of the position calibration member 30. The position calibration means 30 comprises: a slide rail or chute 110 disposed on the calibration body 10;

the slide rail or the chute 110 is also connected with a calibration ruler 90 in a sliding way;

the calibration ruler 90 is further provided with a calibration clamping piece 100 capable of sliding along the calibration ruler 90.

In a specific implementation, since the culture dish is generally circular, in order to ensure that the calibration scale 90 can cover any position of the culture dish in the moving process, the calibration body 10 generally forms a connection platform with a larger area than the inner area of the culture dish, and generally, the connection platform and the culture dish can be a square with a side length larger than the diameter of the culture dish, or the connection platform can be in other shapes beneficial to being held by hand. And a slide rail or chute 110 is provided at an edge position of the connection station. In order to keep the sliding of the calibration ruler 90 stable, two sliding rails or sliding grooves 110 may be provided, and two ends of the calibration ruler 90 are respectively clamped in the sliding grooves or clamped outside the sliding rails and slidably connected with the sliding rails or sliding grooves 110. When the culture dish calibration component is used specifically, the bottom box of the culture dish is firstly installed on the connecting platform through the connecting component 20, and the sliding rail or the sliding groove 110 and the calibration ruler 90 are parallel to the bottom surface of the bottom box of the culture dish. When a certain position in the culture dish needs to be calibrated, the calibration scale 90 can be moved firstly, so that the calibration scale 90 reaches the position needing to be calibrated in advance, and then the calibration clamping piece 100 capable of sliding on the calibration scale 90 is moved, so that the calibration clamping piece 110 also reaches the calibrated position, and the calibration of the position is completed.

In addition, considering that even the transparent scale 90 and the calibration card 100 may affect the subsequent observation of the cells or microorganisms in the culture dish under the microscope, the calibration card 100 is further provided with an observation through hole 120; this allows light to better pass through the viewing aperture 120 to the desired location in the viewing dish for viewing. It should be noted that the corresponding position in the culture dish in the viewing through-hole 120 should be a true calibration position.

Referring to fig. 1, in another embodiment of the present invention, another specific structure of the connection member 20 is further provided, and the connection member 20 includes: a mounting groove 130;

the side wall of the mounting groove 130 is also provided with an anti-skid member 140;

or,

the connection member includes: a clamping mechanism.

Wherein in fig. 1, a mounting slot 130 is shown. The diameter of the mounting groove 130 is slightly larger than that of the bottom box of the culture dish, so that the bottom box of the culture dish can be conveniently placed in the mounting groove 130. In order to ensure that the position of the culture dish is not easily changed in the mounting groove 130, an anti-slip member 140 is further provided on the sidewall of the mounting groove 130.

The anti-slip member 140 may be a ring having an axis of the mounting groove as an axis, or may be a plurality of small anti-slip protrusions uniformly disposed on the inner wall of the mounting groove 130. The antiskid component 140 protrudes from the inner wall of the mounting groove 130, and is made of materials with larger elasticity such as rubber, thus, the friction force between the mounting groove 130 and the bottom box is increased (the friction force between the mounting groove 130 and the bottom box is changed into the friction force between the antiskid component 140 and the bottom box in real time), and meanwhile, the arrangement of the side wall protruding from the mounting groove 130 changes the phase to reduce the mounting space in the mounting groove 130, so that the bottom box is clamped in the mounting groove, the position cannot easily change, but simultaneously, the elasticity of the antiskid component 140 is larger, and inconvenience in mounting the bottom box of the culture dish cannot be caused.

It should be noted that the mounting groove 130 is a through hole disposed on the calibration main body 10; the side wall of the through hole extends inwards to form a limit table 170, and the position calibration hole can penetrate through the through hole and can directly reach the outer side wall of the culture dish bottom box installed in the installation groove 130. In this way, the calibration body 10 does not affect subsequent experimental observations.

In addition, the connecting member may also be a clamping mechanism. The clamping mechanism can be opened and closed like a pair of scissors; the front end of the clamping mechanism forms a clamping ring which is large enough to clamp the culture dish; an anti-slip member is also provided on the inner wall of the clamp ring. At the tail end of fixture, form the centre gripping handle, through opening and shutting of this centre gripping handle control fixture, improve the easy operability of experiment simultaneously.

In addition, referring to fig. 7, the present invention also provides a culture dish, comprising: the method comprises the following steps: a bottom case 150 and a cover 160 covering an opening of the bottom case 150;

the culture dish calibration device provided by the embodiment is further included;

the culture dish calibration device 190 is detachably connected with the bottom box 150.

The utility model has the advantages that:

1. the target position to be observed can be accurately calibrated according to the research needs of experimenters, and dynamic observation of a certain determined target area is facilitated.

2. The utility model provides a culture dish calibration device simple structure, convenient operation.

The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A culture dish calibration device is characterized by comprising: calibrating a main body;

the calibration main body is provided with a connecting component used for being connected with a culture dish;

the calibration main body is also provided with a position calibration component;

the connecting component is also provided with a positioning component used for determining the unique connecting position of the culture dish.

2. The culture dish calibration apparatus as claimed in claim 1, wherein the position calibration member comprises: the rotating disc is rotatably connected with the calibration main body;

and a position calibration hole is formed in the rotating disc.

3. The culture dish calibration device according to claim 2, wherein a slot for installing the position calibration component is further provided on the calibration body.

4. The culture dish calibration device according to claim 3, wherein at least one position calibration hole is provided, and the position calibration hole is at least one of a circular hole, a polygonal hole and an elongated hole.

5. The culture dish calibration device according to claim 4, wherein the rotating disc is circular or fan-shaped;

when the rotating discs are in a fan shape, at least one fan-shaped rotating disc is arranged, and the rotating axes of all the rotating discs are overlapped;

the calibration main body is further provided with a rotating shaft which is rotatably connected with the fan-shaped rotating disc.

6. The culture dish calibration device according to any one of claims 2 to 5, wherein the rotating disc is further provided with a rotating protrusion.

7. The culture dish calibration apparatus as claimed in claim 1, wherein the position calibration member comprises: the sliding rail or the sliding groove is arranged on the calibration main body;

the sliding rail or the sliding chute is also connected with a calibration ruler in a sliding way;

the calibration ruler is also provided with a calibration clamping piece which can slide along the calibration ruler.

8. The culture dish calibration device according to claim 7, wherein the calibration clamp is further provided with an observation through hole.

9. Dish calibration device according to any of claims 1-5, 7-8, wherein the connection member comprises: mounting grooves;

the side wall of the mounting groove is also provided with an anti-skid component;

or,

the connection member includes: a clamping mechanism.

10. A culture dish, comprising: the box comprises a bottom box and a cover covering the opening of the bottom box; the edge of the cover protrudes downwards and can completely cover the opening of the bottom box;

further comprising a culture dish calibration device according to any of the claims 1-9;

the culture dish calibration device is detachably connected with the bottom box.