CN205003084U - Super -resolution imaging system - Google Patents
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- CN205003084U CN205003084U CN201520770102.1U CN201520770102U CN205003084U CN 205003084 U CN205003084 U CN 205003084U CN 201520770102 U CN201520770102 U CN 201520770102U CN 205003084 U CN205003084 U CN 205003084U
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Abstract
The utility model discloses a super -resolution imaging system, including first laser instrument, the second laser instrument, first half -wave plate, first pulse beam splitter, the spectrum appearance, first battery of lens, the glass stick, polarization maintaining fiber, spatial light modulator, first objective, speculum group, the second battery of lens, the speculum, single mode fiber, the second half -wave plate, the retroreflection mirror, the speculum, first double -colored mirror, the double -colored mirror of second, scanning system, the fourth slide, the space objective, second pulse beam splitter and photomultiplier, utilize relevant adaptive optics technique to carry out the spatial resolution that aberration correction improves the micro - imaging system of STED super -resolution, solved because biological the sample surface's the unevenness and the aberration of the interior inhomogeneous introduction of refraction index profile of sample, spatial resolution has been improved.
Description
Technical field
The utility model belongs to optical microphotograph imaging field, more specifically, relates to a kind of method and system improving stimulated emission consume (StimulatedEmissionDepletion, STED) super-resolution micro-imaging spatial resolution.
Background technology
In the optical microphotograph imaging system of routine, due to the diffraction effect of optical component, the hot spot that the illumination light of parallel incidence is formed after microcobjective focuses on sample is not a desirable point, but a diffraction pattern with certain size, according to the Abbe law that roentgen Ernest & Whitney-Abbe proposes, the diameter of the minimum light spot that visible luminous energy focuses on is 1/3rd of optical wavelength, about about 200nm.Within 1994, propose STED super-resolution micro-imaging technique first by Germany scientist S.W.Hell, it has surmounted diffraction limit, and achieves the spatial resolution of 30nm in 2006, and this outstanding work makes him obtain Nobel chemistry Prize in 2014.
The basic thought of STED super-resolution is: utilize stimulated radiation effect to reduce effective fluorescence radiation area, need two-beam in a typical STED microscopic system, a branch of is exciting light, and another bundle is for exhausting light.When exciting light irradiates fluorescent samples, the fluorescence molecule within the scope of its diffraction spot can be made to be excited, electronics wherein will transit to excited state, and then annular exhausted optical superposition on exciting light, exhausting light makes the electronics being in lap excited state get back to ground state in the mode of stimulated radiation, other excited state electronics being positioned at laser spot center, owing to not being subject to exhausting the impact of light, continuing, with the form of spontaneous radiation, fluorescence outwards occurs and gets back to ground state.Because the direction sending fluorescence in stimulated radiation with spontaneous radiation process is different with wavelength, therefore after filtering, being detected the photon that device receives is all produced by the mode of autofluorescence by the fluorescent samples being positioned at exciting light hot spot center.The light-emitting area of so effective fluorescence is reduced, thus improves the spatial resolution of system.
At present, STED super-resolution micro imaging system is in biomedical application, the aberration brought due to the out-of-flatness of sample surfaces and the unevenness of sample interior index distribution makes the resolution of system and imaging depth greatly reduce, and limits its widespread use.
Utility model content
For the defect of prior art, the purpose of this utility model is to utilize relevant adaptive optical technique (CoherentOpticalAdaptiveTechnique, COAT) carry out aberration correction to improve the spatial resolution of STED super-resolution micro imaging system, be intended to solve the aberration introduced due to refractive index skewness in the out-of-flatness on biological sample surface and sample.
The utility model provides a kind of super-resolution imaging system, comprise: the first laser instrument Laser1, second laser Laser2, first half-wave plate, first pulse beam splitter PS1, spectrometer SPEC, first lens combination (L1, L2), glass bar GR, polarization maintaining optical fibre Fiber1, spatial light modulator SLM, first object lens L3, catoptron group, second lens combination (L4, L5), mirror M 4, single-mode fiber Fiber2, second half-wave plate, retroreflector RR, mirror M 5, first dichroic mirror DM1, second dichroic mirror DM2, scanning system Scanner, / 4th slides, space object lens L6, second pulse beam splitter PS2 and photomultiplier PMT, first laser instrument Laser1 is for generation of femtosecond laser, second laser Laser2 is for generation of picosecond laser, first half-wave plate is arranged on the emitting light path of the first laser instrument Laser1, is provided for described femtosecond laser and is linearly polarized light and the direction adjusting linearly polarized light, first pulse beam splitter PS1 is used for the femtosecond laser after the first half-wave plate adjustment to be divided into two-way, and a part of Transmission light enters spectrometer, and the reflection of another part light is as exhausting light, spectrometer SPEC is arranged on the first via emitting light path of the first pulse beam splitter, for exhausting the wavelength of light described in Real-Time Monitoring, first lens combination (L1, L2) is arranged on the second road emitting light path of the first pulse beam splitter, for exhausting light adjustment, glass bar GR is used for carrying out broadening to the light that exhausts after adjustment, makes the pulse width exhausting light be 1 psec, polarization maintaining optical fibre Fiber1 be used for pulse-width be 1 psec exhaust the further broadening of light, the pulse width exhausting light described in making is 200 psecs, spatial light modulator SLM is for generation of annular hot spot and as aberration correction system, first object lens L3 pulse-width be 200 psecs exhaust light adjustment, make the diameter of its hot spot equal the width of liquid crystal panel in spatial light modulator (SLM), between the emergent light that catoptron group is arranged on the first object lens L3 and the incident light of described spatial light modulator, for making it enter described spatial light modulator SLM with the incident angle of 3 ° ~ 9 ° to the emergent light adjustment of the first object lens L3, second lens combination (L4, L5) is for carrying out transmission by the emergent light of spatial light modulator SLM, mirror M 4 is arranged on the transmitted light path of the second lens combination, and its incident light is the emergent light of described second lens combination, single-mode fiber Fiber2 is arranged on the emitting light path of second laser Laser2, for carrying out mode adjustment to picosecond laser, second half-wave plate is arranged on the emitting light path of the first laser instrument Laser1, is provided for described picosecond laser and is linearly polarized light and the direction adjusting linearly polarized light, retroreflector RR is used for reflecting the picosecond laser after the second half-wave plate adjustment, the time delay controlling exciting light and exhaust between light pulse, the incident light of mirror M 5 is the reflected light of retroreflector RR, first incident light of the first dichroic mirror DM1 is exhaust light through M4 reflection, its second incident light is the exciting light through M5 reflection, for reflecting exhausting light, transmission is carried out to exciting light, and the direction that adjustment exhausts light and exciting light makes it overlapping, second dichroic mirror DM2 is arranged on the emitting light path of the first dichroic mirror, for carrying out transmission to exciting light and the described light that exhausts, and reflects fluorescence, scanning system Scanner is arranged on the emitting light path of described second dichroic mirror, for the exciting light of overlap with exhaust light and carry out synchronous scanning, realizes planar array scanning, / 4th slides are used for adjusting the laser after overscanning, are circularly polarized light, space object lens L6 is used for focusing on circularly polarized light and collects the fluorescence signal of sample feedback, second pulse beam splitter PS2 is used for the fluorescence after the second dichroic mirror DM2 reflects to be divided into two parts, and a part of light reflection enters adaptive optics AO aberration correction system, for carrying out real time correction to system aberration, another part light is transmitted, photomultiplier PMT is used for amplifying by the fluorescence after the second pulse beam splitter transmission and carrying out super-resolution imaging.Relevant adaptive optics aberration correction COAT system is made up of jointly spatial light modulator SLM and ADAPTIVE OPTICS SYSTEMS AO, for carrying out real time correction to the aberration of system, improves spatial resolution and the imaging depth of STED system.
Still more preferably, the incident angle of spatial light modulator SLM is 6 °.
Still more preferably, also comprise and to be arranged between described first laser instrument Laser1 and described first half-wave plate and for the protection of the optoisolator FI of laser instrument.
Still more preferably, synchronous trigger the first laser instrument and second laser, and keep being spaced apart 160ps-200ps between two bundle laser pulse peaks.
Still more preferably, 180ps is spaced apart.
Still more preferably, described spatial light modulator (SLM) loads simultaneously for generation of the spiral GTG phase diagram of ring light and the GTG phase diagram for relevant adaptive optics aberration correction.Wherein, spiral GTG phase diagram exhausts light hot spot for generation of annular, the hot spot surmounting diffraction limit is formed after superposing with exciting light, then super resolution image is formed by scanning, and the aberration that the unevenness that relevant adaptive optics aberration correction system is used for correcting sample surface irregularity and sample interior index distribution produces, make the resolution of our super-resolution imaging system higher, imaging depth is darker.
Still more preferably, laser is divided into two-way according to 9:1 by the first pulse beam splitter PS1, and fraction Transmission light enters spectrometer, and most of light reflection is as exhausting light.Fluorescence is divided into two parts according to 9:1 by the second pulse beam splitter PS2, and a part of light reflection enters adaptive optics AO aberration correction system, for carrying out real time correction to system aberration; Another part light is transmitted.
The utility model restraints the phase place of certain light beam in coherent light by regulation and control two, realize the coherent enhancement to two-beam and the relevant manipulation weakened, achieve the aberration correction of microscopic system, thus put forward the spatial resolution of high STED super-resolution micro imaging system, existing super-resolution imaging system can be solved when deep layer imaging biological cells due to the aberration problem that causes picture quality poor that refractive index skewness in the out-of-flatness on biological sample surface and sample is introduced.
Accompanying drawing explanation
Fig. 1 is the light channel structure figure that the utility model implements the super-resolution imaging system provided;
Fig. 2 be exhaust light overlapping with exciting light after surmount the schematic diagram of diffraction limit;
The time interval that Fig. 3 is excitation light pulse and exhausts between light two peak value of pulse;
Fig. 4 is the Confocal image and the STED super resolution image that utilize system to obtain 170nm fluorescent bead;
Fig. 5 is the aberration correction utilizing independent COAT aberration correction system to realize hot spot in scattering sample.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
The method and system of a kind of STED of raising super-resolution imaging spatial resolution that the utility model provides belong to stimulated emission depletion (STED) super-resolution micro imaging system and improve the new method of System spatial resolution, can solve existing super-resolution imaging system when deep layer imaging biological cells due to problem that aberration causes picture quality poor.
The utility model embodiment is achieved in that a kind of super-resolution imaging system, comprising:
Femto-second laser (Laser1), for generation of exhausting light;
Picosecond laser (Laser2), for generation of exciting light;
Optoisolator (FI), for the protection of laser instrument;
Glass bar (GR), exhausts light for broadening femtosecond pulse, makes its pulse width reach about 1 psec;
100 meters of polarization maintaining optical fibres (Fiber1), exhaust light for further broadening, make its pulse width reach 200 picoseconds;
Spectrometer (SPEC), exhausts the correlation parameters such as the wavelength of light for Real-Time Monitoring;
Spatial light modulator (SLM), in the present system, spatial light modulator has two kinds of purposes, and one is the function of serving as helical phase sheet, and for generation of annular hot spot, another effect is for aberration correction system;
Single-mode fiber (Fiber2), for carrying out mode adjustment to the laser of outgoing in picosecond laser;
Retroreflector (RR), for controlling exciting light and exhausting the time delay between light pulse;
Scanning system (Scanner), for the exciting light of overlap with exhaust light and carry out synchronous scanning, realizes planar array scanning;
High numerical space object lens (L6), for focusing on overlapping exciting light and exhausting light, collect fluorescence signal simultaneously; Specifically can adopt numerical aperture be 1.4 Lycra object lens.
Photomultiplier (PMT), for amplifying fluorescence signal;
All herein half-wave plate (
), being all that the laser for ensureing place light path is linear polarization, adjusting the direction of linear polarization simultaneously;
/ 4th slides, make the laser before entering object lens be circularly polarized light;
First pulse beam splitter (PS1), for femtosecond laser being divided into two parts (9:1), fraction Transmission light enters spectrometer, and most of light reflection is as exhausting light;
Second pulse beam splitter (PS2), for the fluorescence signal after collection is divided into two parts (9:1), the reflection of fraction light enters adaptive optics (AO) aberration correction system, for carrying out real time correction to system aberration, major part transmission enters PMT, for super-resolution imaging;
First dichroic mirror (DM1), exhausts light (780nm), transmission exciting light (635nm) for reflection, simultaneously can also finely tune the direction exhausting light, makes to exhaust light and exciting light can be good at overlap;
Second dichroic mirror (DM2), for transmission exciting light and exhaust light, reflected fluorescent light;
Relevant adaptive optics aberration correction (COAT) system, jointly be made up of spatial light modulator (SLM) and ADAPTIVE OPTICS SYSTEMS (AO), for carrying out real time correction to the aberration of system, improve spatial resolution and the imaging depth of the utility model STED system;
During work, first light exciting light sources, after light and exhaust radiant, our way utilizes the control system of femto-second laser synchronously to trigger femto-second laser and picosecond laser, and preferably keep two bundle laser pulse peaks between be spaced apart 160ps-200ps, be preferably 180ps, such guarantee exhaust light cleaner by exciting light produce excited state electronics turn back to ground state with the form of stimulated radiation.In spatial light modulator, we will load for generation of the spiral GTG phase diagram of ring light and the GTG phase diagram for relevant adaptive optics aberration correction simultaneously simultaneously, these two kinds of GTG phase diagrams can be allowed to work simultaneously, not interfereing with each other when testing.Spiral GTG phase diagram exhausts light hot spot for generation of annular, the hot spot surmounting diffraction limit is formed after superposing with exciting light, then super resolution image is formed by scanning, and the aberration that the unevenness that relevant adaptive optics aberration correction system is used for correcting sample surface irregularity and sample interior index distribution produces, make the resolution of our super-resolution imaging system higher, imaging depth is darker.
In order to make, the purpose of this utility model, technical scheme and advantage are more clear to be understood, below in conjunction with drawings and Examples, further describes the utility model.Should be appreciated that specific embodiment described herein only in order to explain the utility model, be not limited to the utility model.
Below in conjunction with embodiment, realization of the present utility model is described in detail.
As shown in Figure 1, the light path system figure of the STED super-resolution micro-imaging based on COAT aberration correction technology provided for the utility model embodiment.From figure, we can see, native system has two bundle laser, be respectively and exhaust light (Laser1 wavelength is 780nm) and exciting light (Laser2 wavelength is 635nm), exhaust light first by optoisolator (FI), optoisolator FI Main Function is here that the reflected light preventing from producing in light path produces harmful effect to laser instrument, and optoisolator FI below
half-wave plate is used to adjustment and exhausts polarisation of light characteristic, guarantee that the light that exhausts entering light path system is linearly polarized light, PS1 is pulse beam splitter, laser is divided into (9:1) two-beam, the light reflection of the overwhelming majority enters subsequent optical path, the light of small part then transmission enters spectrometer (SPEC), spectrometer SPEC mainly carries out Real-Time Monitoring to the laser parameter that laser instrument exports, the light of reflection is then through lens combination (L1, L2) after adjustment, glass bar (GR) is entered with the hot spot of suitable size, glass bar Main Function here carries out broadening to the pulsed light that exhausts of femtosecond, pulse width after broadening is about 1 psec, then the pulsed light that exhausts of broadening is coupled in the polarization maintaining optical fibre of 100 meters long (Fibre1), Main Function utilizes optical fiber paired pulses to exhaust the further broadening of light, final acquisition pulse width 200 psec exhaust light, through polarization maintaining optical fibre Fibre1 adjust after exhaust pulsed light, hot spot adjustment is carried out again through suitable object lens (L3), the size making the size diameter of hot spot and spatial light adjust the width of (SLM) is consistent, then through a series of catoptron (M1, M2, M3) after adjustment, spatial light modulator SLM is entered with the incident angle of 3 ° ~ 9 ° (being preferably 6 °), spatial light modulator SLM effect here mainly contains two, one is produce helical phase gray-scale figure, the light that exhausts through reflection is made to be hollow circular ring shape hot spot, another effect forms COAT aberration correction system with ADAPTIVE OPTICS SYSTEMS (AO) below, aberration correction is carried out to exhausting light, to improve spatial resolution and the imaging depth of STED imaging, through SLM reflection after exhaust light again after lens combination (L4 and L5) carries out hot spot adjustment, scanning system Scanner is entered with suitable spot size, then enter 1/4th slides (
), linearly polarized light is transformed into circularly polarized light, eventually passes through object lens adjustment and enter sample.
Wherein, the size of hot spot will decide according to selected galvanometer, so-called suitable spot size, refer to that hot spot just aims at the center of galvanometer two mirror surfaces, and there are 1/4th minute surface width at the edge of hot spot (because the X of galvanometer to the edge of minute surface, the vibration plane of Y is the catoptron of rectangle, and therefore main discussion here is the wide of rectangle), namely spot size will occupy the position at the center 1/2nd of minute surface.
Exciting light accesses single-mode fiber Fiber2 by picosecond laser Laser2 through overcoupling, after adjusting, passes through the pattern of exciting light
half-wave plate, the linear polarization state of exciting light is adjusted, then through the adjustment of catoptron (RR and M5) and dichroic mirror (DM1 and DM2), at scanning system Scanner place with to exhaust light overlapping, mirror M 5 and dichroic mirror DM1, and light can be exhausted carry out small angle vernier adjustable exciting light, to guarantee that two-beam height overlaps, as shown in Figure 2.Want to realize super-resolution imaging in STED super-resolution system, must be kept having an appointment between exciting light and the peak value of pulse exhausting light time interval of 180 psecs, as shown in Figure 3, in the process of experiment, in order to keep stable synchronism, with the pulse interval that generation is such, we carry out and carry out trigger laser Laser2 simultaneously by the control system of laser instrument Laser1 by external connection, then realized by the position changing retroreflector RR in the length of outer contacting hair line cable and adjustment excitation light path that (length and the time interval of outer contacting hair line cable are linear in time interval of 180 psecs, length is longer, the time interval is also longer, such as, the light velocity is 3*10
8m/s, after converting, known often mobile one meter of time delay that can change was 3.333 nanoseconds).Exciting light after overlap and exhaust light through object lens focus on after, fluorescence excitation sample, the fluorescence signal that sample produces, collected by object lens again, return along original optical path, reflected at dichroic mirror DM2 place, two-beam is divided into after beam splitting chip (9:1), by being coupled into multimode optical fiber (because the optical fiber port of single-mode fiber is very little after major part Transmission light, therefore the effect of aperture can be played), and then introduce photomultiplier PMT by multimode optical fiber, carry out signal amplification, last imaging on computers, fraction light then enters AO system after reflection, relevant adaptive optics aberration correction system is jointly formed with SLM above, this system can correct the aberration that biological sample is introduced in real time, thus improve spatial resolution and the imaging depth of STED super-resolution system.
Diameter is that the fluorescent bead of 170nm is as laboratory sample by we in an experiment, we in the light path exhausting light (between M4 and DM1) arranges one piece of automatically controlled baffle plate, when the shutters are closed, baffle plate can block and exhaust light, exciting light is only had to enter object lens, now can be considered Confocal imaging, when the flaps are opened, exhausting light can be overlapping on sample with exciting light, form STED super-resolution imaging, as shown in Figure 4, for the fluorescent bead image of the 170nm that we gather by this STED super-resolution imaging system (not opening COAT aberration correction), the Confocal image of fluorescent bead and STED image in comparison diagram, we can significantly see, after exhausting light action, the point spread function of exciting light, really obvious reduction is had.Although STED super-resolution imaging system, the spatial resolution of 30nm has just been achieved as far back as 2006, but in biological sample, due to the unevenness that out-of-flatness and the sample interior refractive index of sample surfaces are differentiated, larger aberration can be introduced to STED super-resolution system, the spatial resolution of reduction system and imaging depth, in order to overcome this problem, we have proposed and introduce COAT aberration correction system in STED super-resolution imaging system, as shown in Figure 5, for we utilize independent COAT aberration correction system to realize the aberration correction of hot spot in scattering sample, therefore after COAT introduces by we in the present system, the aberration introduced in system should be overcome, realize the raising of STED super-resolution spatial resolution and imaging depth.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (8)
1. a super-resolution imaging system, is characterized in that, comprising:
First laser instrument Laser1, for generation of femtosecond laser;
Second laser Laser2, for generation of picosecond laser;
First half-wave plate, is arranged on the emitting light path of described first laser instrument Laser1, is provided for described femtosecond laser and is linearly polarized light and the direction adjusting linearly polarized light;
First pulse beam splitter, for the femtosecond laser after described first half-wave plate adjustment is divided into two-way, a part of Transmission light enters spectrometer, and the reflection of another part light is as exhausting light;
Spectrometer, is arranged on the first via emitting light path of described first pulse beam splitter, for exhausting the wavelength of light described in Real-Time Monitoring;
First lens combination, is arranged on the second road emitting light path of described first pulse beam splitter, for exhausting light adjustment;
Glass bar, for carrying out broadening to the light that exhausts after adjustment, the pulse width exhausting light described in making is 1 psec;
Polarization maintaining optical fibre, for pulse-width be 1 psec exhaust the further broadening of light, the pulse width exhausting light described in making is 200 psecs;
Spatial light modulator, for generation of annular hot spot and as aberration correction system;
First object lens L3, pulse-width be 200 psecs exhaust light adjustment, make the diameter of its hot spot equal the width of liquid crystal panel in spatial light modulator;
Catoptron group, between the emergent light being arranged on described first object lens L3 and the incident light of described spatial light modulator, for making it enter described spatial light modulator with the incident angle of 3 ° ~ 9 ° to the emergent light adjustment of described first object lens L3,
Second lens combination, for carrying out transmission by the emergent light of described spatial light modulator SLM;
Mirror M 4, is arranged on the transmitted light path of described second lens combination, and its incident light is the emergent light of described second lens combination;
Single-mode fiber, is arranged on the emitting light path of described second laser Laser2, for carrying out mode adjustment to described picosecond laser;
Second half-wave plate, is arranged on the emitting light path of described first laser instrument Laser1, is provided for described picosecond laser and is linearly polarized light and the direction adjusting linearly polarized light;
Retroreflector, for reflecting the picosecond laser after described second half-wave plate adjustment, the time delay controlling exciting light and exhaust between light pulse;
Mirror M 5, its incident light is the reflected light of described retroreflector;
First dichroic mirror, its first incident light be reflect through described mirror M 4 exhaust light, its second incident light is the exciting light reflected through described mirror M 5, for reflecting the described light that exhausts, transmission is carried out to described exciting light, and the direction exhausting light and described exciting light described in adjustment makes it overlapping;
Second dichroic mirror, is arranged on the emitting light path of described first dichroic mirror, for carrying out transmission to described exciting light and the described light that exhausts, and reflects fluorescence;
Scanning system, is arranged on the emitting light path of described second dichroic mirror, for the exciting light of overlap with exhaust light and carry out synchronous scanning, realizes planar array scanning;
/ 4th slides, for adjusting the laser after overscanning, are circularly polarized light;
Space object lens L6, for described circularly polarized light is focused on and collect sample feedback fluorescence signal;
Second pulse beam splitter, for the fluorescence after described second dichroic mirror reflection is divided into two parts, a part of light reflection enters adaptive optics aberration correction system, for carrying out real time correction to system aberration; Another part light is transmitted;
Photomultiplier, for amplifying by the fluorescence after described second pulse beam splitter transmission and carry out super-resolution imaging.
2. super-resolution imaging system as claimed in claim 1, it is characterized in that, the incident angle of described spatial light modulator SLM is 6 °.
3. super-resolution imaging system as claimed in claim 1, is characterized in that, also comprises being arranged between described first laser instrument Laser1 and described first half-wave plate and for the protection of the optoisolator of laser instrument.
4. super-resolution imaging system as claimed in claim 1, is characterized in that, synchronously triggers the first laser instrument and second laser, and keeps being spaced apart 160ps-200ps between two bundle laser pulse peaks.
5. super-resolution imaging system as claimed in claim 4, is characterized in that, described in be spaced apart 180ps.
6. the super-resolution imaging system as described in any one of claim 1-5, is characterized in that, described spatial light modulator loads simultaneously for generation of the spiral GTG phase diagram of ring light and the GTG phase diagram for relevant adaptive optics aberration correction.
7. the super-resolution imaging system as described in any one of claim 1-5, is characterized in that, laser is divided into two-way according to 9:1 by described first pulse beam splitter, and fraction Transmission light enters spectrometer, and most of light reflection is as exhausting light.
8. the super-resolution imaging system as described in any one of claim 1-5, it is characterized in that, fluorescence is divided into two parts according to 9:1 by described second pulse beam splitter, and a part of light reflection enters adaptive optics aberration correction system, for carrying out real time correction to system aberration; Another part light is transmitted.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105241857A (en) * | 2015-09-30 | 2016-01-13 | 深圳大学 | Super-resolution imaging system |
| CN106483103A (en) * | 2016-12-01 | 2017-03-08 | 上海电机学院 | A kind of ultrafast process imaging device of high-space resolution |
| CN107102317A (en) * | 2017-06-20 | 2017-08-29 | 北醒（北京）光子科技有限公司 | A kind of device for measuring angular resolution |
| CN108681095A (en) * | 2018-05-21 | 2018-10-19 | 武汉华日精密激光股份有限公司 | Pulse shortener and femtosecond pulse laser |
| CN108680548A (en) * | 2018-05-16 | 2018-10-19 | 华中科技大学 | A kind of full fiber type super-resolution imaging method and device |
| CN110118726A (en) * | 2019-04-12 | 2019-08-13 | 浙江大学 | A kind of method and apparatus of parallel detecting fluorescent emission difference micro-imaging |
| CN113484297A (en) * | 2021-09-07 | 2021-10-08 | 清华大学 | Fluorescent light sheet microscope system |
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2015
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105241857A (en) * | 2015-09-30 | 2016-01-13 | 深圳大学 | Super-resolution imaging system |
| CN106483103A (en) * | 2016-12-01 | 2017-03-08 | 上海电机学院 | A kind of ultrafast process imaging device of high-space resolution |
| CN107102317A (en) * | 2017-06-20 | 2017-08-29 | 北醒（北京）光子科技有限公司 | A kind of device for measuring angular resolution |
| CN107102317B (en) * | 2017-06-20 | 2023-10-13 | 北醒（北京）光子科技有限公司 | Device for measuring angular resolution |
| CN108680548A (en) * | 2018-05-16 | 2018-10-19 | 华中科技大学 | A kind of full fiber type super-resolution imaging method and device |
| CN108680548B (en) * | 2018-05-16 | 2019-12-06 | 华中科技大学 | all-fiber super-resolution imaging method and device |
| CN108681095A (en) * | 2018-05-21 | 2018-10-19 | 武汉华日精密激光股份有限公司 | Pulse shortener and femtosecond pulse laser |
| CN110118726A (en) * | 2019-04-12 | 2019-08-13 | 浙江大学 | A kind of method and apparatus of parallel detecting fluorescent emission difference micro-imaging |
| CN113484297A (en) * | 2021-09-07 | 2021-10-08 | 清华大学 | Fluorescent light sheet microscope system |
| CN113484297B (en) * | 2021-09-07 | 2021-12-24 | 清华大学 | Fluorescent light sheet microscope system |
| CN114895450A (en) * | 2022-05-10 | 2022-08-12 | 深圳大学 | Super-resolution microscopic imaging system and imaging method based on second harmonic |
| CN114895450B (en) * | 2022-05-10 | 2023-05-19 | 深圳大学 | Super-resolution microscopic imaging system and method based on second harmonic |
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