CN204939479U - A kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism - Google Patents
A kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism Download PDFInfo
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- CN204939479U CN204939479U CN201520557060.3U CN201520557060U CN204939479U CN 204939479 U CN204939479 U CN 204939479U CN 201520557060 U CN201520557060 U CN 201520557060U CN 204939479 U CN204939479 U CN 204939479U
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Abstract
The utility model provides a kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism, described chip comprises chip body, described chip body there are 12 grooves, be respectively: Baird-Parker substratum groove, Yihong methylene blue substratum groove, BETA-SSA substratum groove, streptococcus agalactiae color developing culture medium groove, B race streptococcus cultures groove, klebsiella color developing culture medium groove, KF streptococcus cultures groove, PALCAM substratum groove, sramana Shigella nutrient agar groove, hexadecane trimethyl ammonium bromide nutrient agar groove, Sabouraud's agar substratum groove, nutrient agar groove.Chip of the present utility model can detect the various pathogenic micro-organism that causes mastadenitis of cow and the residual various pathogenic micro-organisms of milk simultaneously, greatly reduces the labour intensity of testing staff, decreases the consumption of substratum, reduce cost.
Description
Technical field
The utility model relates to a kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism.
Background technology
Mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism have multiple, PI pathogenic microorganism spawn culture authenticate technology is after utilizing selective medium to cultivate, observe colonial morphology to identify, but it is low that the method detects flux, one flat plate once identifies at most 2-3 kind bacterial classification, required culture medium flat plate quantity is many, and cost is large, length consuming time.
Utility model content
In order to solve problems of the prior art, the utility model provides a kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism, the various Residual Disease pathogenic microorganisms causing the various pathogenic micro-organism of mastadenitis of cow and milk can be detected simultaneously, convenient and swift, decrease the consumption of substratum, reduce the labour intensity of testing staff, cost effectively reduces.
The utility model provides a kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism, described chip comprises chip body (3), described chip body (3) there are 12 grooves (6), be respectively: for placing the Baird-Parker substratum groove (601) of Baird-Parker substratum, for placing Yihong methylene blue substratum groove (602) of Yihong methylene blue substratum, for placing the BETA-SSA substratum groove (603) of BETA-SSA substratum, for placing the streptococcus agalactiae color developing culture medium groove (604) of streptococcus agalactiae color developing culture medium, for placing B race streptococcus cultures groove (605) of B race streptococcus cultures, for placing the klebsiella color developing culture medium groove (606) of klebsiella color developing culture medium, for placing the KF streptococcus cultures groove (607) of KF streptococcus cultures, for placing the PALCAM substratum groove (608) of PALCAM substratum, for placing sramana Shigella nutrient agar groove (609) of sramana Shigella nutrient agar, for placing the hexadecane trimethyl ammonium bromide nutrient agar groove (610) of hexadecane trimethyl ammonium bromide nutrient agar, for placing the Sabouraud's agar substratum groove (611) of Sabouraud's agar substratum, for placing the nutrient agar groove (612) of nutrient agar.
Preferably, described chip body (3) there are two row's grooves (6).
Preferably, described chip also comprises reagent bottle (1) and reagent lid (2), there is the cylindrical protrusions (4) of a hollow described reagent lid (2) center of inside position, described chip body (3) is the platy structure with handle (5), handle (5) can insert in the cylindrical protrusions (4) of reagent lid (2) fixing, when being inserted after in cylindrical protrusions (4) by handle (5), reagent bottle (1) and reagent lid (2) can screw up or fasten.
The utility model is for current mastadenitis of cow pathogenic micro-organism bacterial classification and the inefficient shortcoming of milk Residual Disease pathogenic microorganism identification, provide a kind of mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism detection chip, this chip can detect the various pathogenic micro-organism that causes mastadenitis of cow and the residual various pathogenic micro-organisms of milk simultaneously, greatly reduce the labour intensity of testing staff, decrease the consumption of substratum, reduce cost.Meanwhile, cause the substratum of the Different Kinds of Pathogens microorganism of mastadenitis of cow Residual Disease pathogenic microorganism different from milk to be incorporated on a chip detection, using also can be convenient.
Accompanying drawing explanation
Accompanying drawing is used to provide further understanding of the present utility model, and forms a part for specification sheets, is used from explanation the utility model, does not form restriction of the present utility model with embodiment one of the present utility model.In the accompanying drawings:
Fig. 1 is the structure iron of reagent bottle;
Fig. 2 is the front view of reagent lid;
Fig. 3 is the left view of reagent lid;
Fig. 4 is the front view of chip body;
Wherein, 1 is reagent bottle; 2 is reagent lid; 3 is chip body; 4 is cylindrical protrusions; 5 is handle; 6 is groove.
Embodiment
Following embodiment is convenient to understand the utility model better, but does not limit the utility model.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is commercially available.
embodiment 1
The chip being used for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism of the present utility model comprises reagent bottle 1, reagent lid 2 and chip body 3; There is the cylindrical protrusions 4 of a hollow reagent lid 2 center of inside position, chip body 3 is the platy structure with handle 5, and there is certain thickness (such as 5mm-25mm, concrete thickness is as the criterion can place corresponding substratum), handle 5 can insert in the cylindrical protrusions 4 of reagent lid fixing, when being inserted after in cylindrical protrusions 4 by handle 5, reagent bottle 1 and reagent lid 2 still can screw up or fasten.
Chip body 3 there are two row's grooves 6, totally 12, be respectively in order: for placing the Baird-Parker substratum groove 601 of Baird-Parker substratum, for placing Yihong methylene blue substratum groove 602 of Yihong methylene blue substratum, for placing the BETA-SSA substratum groove 603 of BETA-SSA substratum, for placing the streptococcus agalactiae color developing culture medium groove 604 of streptococcus agalactiae color developing culture medium, for placing the B race streptococcus cultures groove 605 of B race streptococcus cultures, for placing the klebsiella color developing culture medium groove 606 of klebsiella color developing culture medium, for placing the KF streptococcus cultures groove 607 of KF streptococcus cultures, for placing the PALCAM substratum groove 608 of PALCAM substratum, for placing the sramana Shigella nutrient agar groove 609 of sramana Shigella nutrient agar, for placing the hexadecane trimethyl ammonium bromide nutrient agar groove 610 of hexadecane trimethyl ammonium bromide nutrient agar, for placing the Sabouraud's agar substratum groove 611 of Sabouraud's agar substratum, for placing the nutrient agar groove 612 of nutrient agar.
During use, reagent bottle is opened, the chip body of taking off reagent lid and being flexibly connected with reagent lid, get 5ml milk sample 10000 to be checked and leave the heart 10 minutes, abandon supernatant, precipitation 10mlPBS solution suspension evenly makes suspension, suspension smeared to respectively each media surface with aseptic cotton carrier or swab (as too high in concentration of specimens to be checked, use after suspension is carried out gradient dilution by suggestion), cultivate, observe colony colour change and identify contained pathogenic bacteria in thing to be checked.Concrete colour-change is see table 1.
Detection chip of the present utility model of applying table 1 detects colony colour change during different strain
。
Last it is noted that the foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, although be described in detail the utility model with reference to previous embodiment, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.All within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (1)
1. one kind for detecting the chip of mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism, it is characterized in that: described chip comprises chip body (3), described chip body (3) there are 12 grooves (6), be respectively: for placing the Baird-Parker substratum groove (601) of Baird-Parker substratum, for placing Yihong methylene blue substratum groove (602) of Yihong methylene blue substratum, for placing the BETA-SSA substratum groove (603) of BETA-SSA substratum, for placing the streptococcus agalactiae color developing culture medium groove (604) of streptococcus agalactiae color developing culture medium, for placing B race streptococcus cultures groove (605) of B race streptococcus cultures, for placing the klebsiella color developing culture medium groove (606) of klebsiella color developing culture medium, for placing the KF streptococcus cultures groove (607) of KF streptococcus cultures, for placing the PALCAM substratum groove (608) of PALCAM substratum, for placing sramana Shigella nutrient agar groove (609) of sramana Shigella nutrient agar, for placing the hexadecane trimethyl ammonium bromide nutrient agar groove (610) of hexadecane trimethyl ammonium bromide nutrient agar, for placing the Sabouraud's agar substratum groove (611) of Sabouraud's agar substratum, for placing the nutrient agar groove (612) of nutrient agar, described chip body (3) there are two row's grooves (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520557060.3U CN204939479U (en) | 2015-07-29 | 2015-07-29 | A kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520557060.3U CN204939479U (en) | 2015-07-29 | 2015-07-29 | A kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism |
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| CN204939479U true CN204939479U (en) | 2016-01-06 |
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| CN201520557060.3U Active CN204939479U (en) | 2015-07-29 | 2015-07-29 | A kind of chip for detecting mastadenitis of cow pathogenic micro-organism and milk Residual Disease pathogenic microorganism |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106480161A (en) * | 2016-11-16 | 2017-03-08 | 吉林大学 | A kind of mastitis for milk cows detection kit |
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2015
- 2015-07-29 CN CN201520557060.3U patent/CN204939479U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106480161A (en) * | 2016-11-16 | 2017-03-08 | 吉林大学 | A kind of mastitis for milk cows detection kit |
| CN106480161B (en) * | 2016-11-16 | 2018-04-03 | 吉林大学 | A kind of mastitis for milk cows detection kit |
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Effective date of registration: 20220601 Address after: 730000 a01-a08, 20 / F, 102 Yandong Road, Chengguan District, Lanzhou City, Gansu Province Patentee after: LANZHOU BAIYUAN GENE TECHNOLOGY Co.,Ltd. Address before: 730000 room 1703, zone a, innovation building, innovation park, No. 268, Yannan Road, Chengguan District, Lanzhou City, Gansu Province Patentee before: LANZHOU LIANGJIANG AGRICULTURAL TECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |