CN204924948U - DTNBP1 chemiluminescence method detect reagent box - Google Patents

Abstract

The utility model discloses a DTNBP1 chemiluminescence method detect reagent box, this kit includes: the standard substance solution of micropore board, enzyme mark antibody working solution, seven concentration, the luminous liquid A of substrate, the luminous liquid B of substrate, peridium buffer solution. The utility model discloses a DTNBP1 antibody is wrapped in advance to the chemiluminescence method on micropore board micropore strip, with the DTNBP1 antigen binding of sample median, two resistive connections of the latter and horseradish peroxidase mark close, and is luminous with the luminous liquid of substrate, utilizes luminous value rather than the volume of the containing DTNBP1 relation of being directly proportional, can reach DTNBP1's in the sample content. The utility model has the characteristics of high sensitivity etc. Fast, exert the important function in DTNBP1's the content in detecting the biological sample.

Description

DTNBP1 chemoluminescence method detection kit

Technical field

The utility model relates to a kind of new chemical luminescence method detection technique field, specifically a kind of serum DTNBP1 chemiluminescence detection kit.

Background technology

DTNBP1 gene is positioned at 6p22.3, mankind DTNBP1 full length gene 140kb, comprises 1 promoter and 10 extrons.The albumen of DTNBP1 gene code is about 40kDa ~ 50kDa, is made up of 352 amino acid, has 1 Coiled-coil secondary structure, is a kind of upper conservative albumen of evolving.DTNBP1 albumen all has expression in the Various Tissues such as neural soleplate, hippocampus, cerebellum of human body, mainly be distributed in the presynaptic Nerve Terminals In The Human Skin of Glutamatergic passage, participate in setting up and maintain adjustment that between contacting between neuron and myocyte, cynapse, chemical signal transmits and the informational linkage between support and adjustment cerebral neuron.Research shows that DTNBP1 gene and schizophrenia exist and maintains close ties with, and the DTNBP1 expression in schizophreniac's gyrus significantly declines, and prompting DTNBP1 may be a schizoid tumor susceptibility gene and candidate's function factor.This seminar premenstruum (premenstrua), research found, the expression of DTNBP1 in alimentary canal patients serum and tumor tissues is significantly raised, and pointed out it may be relevant with the generation development of cancer of pancreas, may become a potential tumor markers.But at present for DTNBP1 detect and depend on multiplex PCR amplification (PCR) and immunohistochemistry (IHC), process is loaded down with trivial details and efficiency is not high.Therefore, invent a kind of quick, efficient detection means so that the Big Clinical Samples of DTNBP1 screens and analyzes, have important effect for the function and clinical meaning setting forth DTNBP1.

Mainly contain radiommunoassay, enzyme-linked immunoassay, latex agglutination test etc. for clinical biochemistry indications method after testing at present, but all there is obvious limitation and defect in these methods.Although radiommunoassay has higher sensitivity and specificity, its term of validity is shorter, and has radioactive contamination; Enzyme-linked immunoassay is current main detection means, and technology is more ripe, application is comparatively wide and cost is lower, but its reaction substrate great majority have toxicity or potential carcinogenicity; Latex agglutination test then has higher subjectivity, and resultant error is large.

Chemiluminometry (ChemiLuminescence, referred to as CL) be the principle of chemiluminescence intensity linearly quantitative relationship under certain condition according to testing concentration in chemical detection system and system, utilize instrument to detect system chemiluminescence intensity, and determine that the one of determinand content weighs analytical approach.Along with development gradually and the maturation of chemiluminescence, its application is more and more extensive.Especially at biomedical sector, chemiluminescence obtains the universal of certain degree, has been widely used in the clinical detection of various bioactivators, as applied chemistry luminescence method detects hepatitis B, thyroid function and tumor markers etc.Chemiluminometry is highly sensitive with it, the range of linearity is wide, analysis realizes the advantages such as robotization fast and easily, to become in analytical chemistry one already and extremely talk about the study hotspot jumped, and will radiommunoassay and enzyme-linked immuno assay etc. be replaced, become the main development direction of modern immunodiagnosis.

As can be seen here, the serology how chemical luminescence detection method being applied to DTNBP1 quantitatively detects, and according to testing result, the severity extent of alimentary tumor patient, result for the treatment of and prognosis are judged and predicted, significant to the large sample examination of the clinical practice and tumor-related illness that expand DTNBP1.

Summary of the invention

The purpose of this utility model is to provide a kind of DTNBP1 chemoluminescence method detection kit, can accurately, DTNBP1 content in the detection serum of quick safety again.

The technical solution of the utility model is: a kind of DTNBP1 chemoluminescence method detection kit, it is characterized in that: comprise one piece of 96 hole microwell plate, ten bottles of reagent, one bag of solid phosphoric acid-tween bag in box body and box and be buffered liquid, in 96 wherein said hole microwell plates, be provided with pre-coated DTNBP1 antibody micropore; Ten bottles of reagent are respectively the standard solution of seven concentration, one bottle of enzyme labelled antibody working fluid, one bottle of substrate luminescent solution A, one bottle of substrate luminescent solution B, wherein one bottle of enzyme labelled antibody working fluid and one bottle of black lid PE translucent plastic bottle of substrate luminescent solution A, one bottle of black lid PE black plastic bottle of substrate luminescent solution B, described box body is carton box, 96 hole microwell plates are placed in vacuum Fresco Bag, and bag is buffered liquid and is placed in sealing bag.

96 described hole microwell plates are made up of outer gimbal support and dismountable eight the enzyme mark bars be placed on it, and each dismountable enzyme mark bar has 12 micropores.

The purpose of this utility model is achieved in that based on double antibody sandwich method, on microwell plate, pre-coated DTNBP1 monoclonal antibody catches serum DTNBP1 antigen, add HRP again and mark DTNBP1 antibody, form antibody-DTNBP1 Ag-Ab HRP marker complex, wash plate and add luminol-H ₂o ₂reaction reagent, measures luminous value with chemiluminescent analyzer.In serum, DTNBP1 content is higher, in conjunction with enzyme labelled antibody more, then chemiluminescence intensity is stronger; Vice versa.Therefore, the level of DTNBP1 in indirect quantification human serum can be carried out according to the chemiluminescence intensity detected.By result compared with normal health check-up blood serum values, thus judge yin and yang attribute.

Reagent forms

1. luminous substrate: A liquid is H ₂o ₂, B liquid is luminol.

2. bag is buffered liquid: 0.01M, pH7.4PBS-Tween20 damping fluid.

The beneficial effects of the utility model be can more fast, the content of Sensitive Detection serum DTNBP1.

Accompanying drawing explanation

Fig. 1 is the utility model box body schematic diagram.

Fig. 2 is the schematic diagram of the utility model 96 hole microwell plate.

Fig. 3 is the schematic diagram that the utility model bag is buffered liquid.

Fig. 4 is the utility model reagent bottle schematic diagram.

Fig. 5 is the utility model one-piece construction schematic diagram.

Wherein 1 is box body; 2 is 96 hole microwell plates of pre-coated DTNBP1 antibody; 3 are buffered liquid for bag; 4 is standard solution, and 5 is enzyme labelled antibody working fluid, and 6 is substrate luminescent solution A; 7 is substrate luminescent solution B.

Embodiment

As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4 and Fig. 5, a kind of DTNBP1 chemoluminescence method detection kit, comprising one piece of 96 hole microwell plate 2,96 hole microwell plate in box body 1 and box is solid carrier, pre-coated DTNBP1 antibody micropore; Ten bottles of reagent, are respectively seven bottles of standard solutions, 4, one bottle of enzyme labelled antibody working fluid, 5, one bottle of substrate luminescent solution A6, one bottle of substrate luminescent solution B7.Box body 1 is carton box, and 96 hole microwell plates 2 are placed in vacuum Fresco Bag, one bottle of enzyme labelled antibody working fluid 5 and one bottle of black lid PE translucent plastic bottle of substrate luminescent solution A6, one bottle of black lid PE black plastic bottle of substrate luminescent solution B7.96 hole microwell plates 2 are made up of outer gimbal support and dismountable eight the enzyme mark bars be placed on it, and each dismountable enzyme mark bar has 12 micropores.

Add in the microwell plate of pre-coated DTNBP1 antibody by human plasma by 50 μ l/ holes, water-bath, wash plate, add enzyme labelled antibody working fluid 50 μ l/ hole, wash plate, add luminous A, B liquid of substrate, Chemiluminescence Apparatus detects, and obtains DTNBP1 concentration.

Claims (2)

1. a DTNBP1 chemoluminescence method detection kit, it is characterized in that: comprise one piece of 96 hole microwell plate (2), ten bottles of reagent, one bag of solid phosphoric acid-tween bag in box body (1) and box and be buffered liquid (3), in 96 wherein said hole microwell plates, be provided with pre-coated DTNBP1 antibody micropore; Ten bottles of reagent are respectively the standard solution (4) of seven concentration, one bottle of enzyme labelled antibody working fluid (5), one bottle of substrate luminescent solution A (6), one bottle of substrate luminescent solution B (7), wherein one bottle of enzyme labelled antibody working fluid (5) and one bottle of substrate luminescent solution A (6) are with black lid PE translucent plastic bottle, one bottle of substrate luminescent solution B (7) is with black lid PE black plastic bottle, described box body (1) is carton box, 96 hole microwell plates (2) are placed in vacuum Fresco Bag, and bag is buffered liquid (3) and is placed in sealing bag.

2. a kind of DTNBP1 chemoluminescence method detection kit according to claim 1, it is characterized in that: 96 described hole microwell plates (2) are made up of outer gimbal support and dismountable eight the enzyme mark bars be placed on it, and each dismountable enzyme mark bar has 12 micropores.