CN204903546U - A pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit - Google Patents
A pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit Download PDFInfo
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- CN204903546U CN204903546U CN201520548925.XU CN201520548925U CN204903546U CN 204903546 U CN204903546 U CN 204903546U CN 201520548925 U CN201520548925 U CN 201520548925U CN 204903546 U CN204903546 U CN 204903546U
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Abstract
The utility model provides a pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit, comprising a base plate, the middle part of bottom plate sets up nitrocellulose membranes, nitrocellulose membranes is last to be provided with the alternate segregation and to control the goat -anti rabbit igG matter accuse line of arranging in proper order, CCP antigen detection line and goat -anti mouse igG matter accuse line, goat -anti rabbit igG matter accuse line, CCP antigen detection line and goat -anti mouse igG matter accuse solenoid are by on nitrocellulose membranes, one side of sample pad covers at nitrocellulose membranes on this one side that goat -anti rabbit igG matter accuse line was located, it covers on nitrocellulose membranes's opposite side to absorb water the one side of filling up. The utility model discloses can be applied to but not be limited to semi -automatic and full -automatic POCT immunoassay instrument, the survey time is short, and the result is accurate, sensitivity is high. Cost to single sample and a large amount of sample test is almost the same, consequently is applicable to detection a small amount of and the batch sample.
Description
Technical field
The utility model relates to a kind of for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit and check-out console thereof.
Background technology
Rheumatoid arthritis is also known as quasi-wind gateway (RA), and RA is a kind of chronic multi-joint damage caused with the misgrowth of joint tissue or pannus is the system inflammation disease of feature, causes random joints lose function.The joint injury of nearest research display RA patient appears at after disease starts 2 years, and early stage effectively treatment can prevent the progress subsequently of this disease.Therefore, early diagnosis and early treatment become to attach most importance to.But RA patient does not always demonstrate typical symptom and phenomenon in early days in disease because their may and not in full conformity with RA diagnostic criteria, this just makes diagnosis become difficulty.RA is because of the autoantibody of IgGFc section corresponding to RF, and the appearance of other antibody and be divided in systemic autoimmune disease, and so far, RF is applied to the unique serologic marker thing of clinical RA.But the susceptibility of RF in RA is between 60-80%, its specificity is also quite low, because RF also can extensively and continually be identified in other diseases, comprises various connective tissue disease, chronic hepatic diseases and infectious diseases, even in the middle of minority healthy population.Therefore RF is applied to the diagnostic criteria of RA, however it early diagnosis be worth and unsatisfactory.
Recent years, several new antibody is found in RA patient, and its clinical characters and possible pathological role also present.Particularly new anti-citrulline albumen autoantibody, as filaggrin and its cyclic versions (i.e. cyclic citrullinated peptide: CCP) acceptable susceptibility and specificity highly and receive much concern in RA patient, therefore becomes the mark of early diagnosis and the omen factor of joint injury.Nearest research has confirmed that RA patient not only produces the RF factor and also has other various different antibody.Although most of antibody is non-specific for RA, but citrulline albumen autoantibody (APF, AKA, anti-filaggrin antibody, antiCCP antibody and anti Sa antibody) is only identified in RA, antiCCP antibody because of its height Sensitivity and Specificity especially cause concern.AntiCCP antibody is diagnosis RA, particularly the first-selected index of early stage RA, is applicable to the RA being difficult to make a definite diagnosis and the diagnosis needing antidiastole RA and other inflammatory arthritis and other forms of panarthritis, can confirms the RA of serology RF feminine gender simultaneously.Be in particular in following four aspects: 1. antiCCP antibody is to the accuracy of clinical diagnosis RA: specificity 95%, susceptibility 80%; 2. the RA patient that antiCCP antibody can be gone to a doctor first at majority detects-appears at early stage RA patient; 3. antiCCP antibody titre relevant to Disease Activity-indication lapse to; 4. continue to monitor antiCCP antibody as the rodent progress index of early stage RA.
The detection method of current anti-cyclic citrullinated peptide (CCP) antibody is enzyme linked immunosorbent assay (ELISA), and commercialization antiCCP antibody detection kit is mainly ELISA kit in the market.ELISA method running program is loaded down with trivial details, complete whole experimentation and need three hours, also professional immunological technique personnel are needed to carry out experimental implementation in the lab, and experimental result need be read after microplate reader detects, in this laboratory at basic medical unit and small-sized outpatient service, the more difficult experiment realizing this project detects, be subject to the impact of the environmental baseline factors such as various temperature and incubation time simultaneously based on ELISA method, inconvenience is brought to test.
Summary of the invention
The purpose of this utility model is to provide a kind of check-out console for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit, simple to operate, accurate, quick, sensitive special.
To achieve these goals, the utility model takes following technical scheme:
For a check-out console for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit, it is characterized in that:
Comprise base plate, the middle part of base plate arranges nitrocellulose filter, nitrocellulose filter is provided with and is separated from each other and left and right goat anti-rabbit igg nature controlling line, CCP antigen detection line and the sheep anti-mouse igg nature controlling line arranged successively, described goat anti-rabbit igg nature controlling line, CCP antigen detection line and sheep anti-mouse igg nature controlling line are coated on nitrocellulose filter, the side of sample pad covers nitrocellulose filter on this side residing for goat anti-rabbit igg nature controlling line, and the side of adsorptive pads covers on the opposite side of nitrocellulose filter.
Further, described check-out console has shell, is plastic material, described shell two pieces of dividing upper cover and lower cover to link together, and upper cover is provided with well and detection window; The position at the corresponding goat anti-rabbit igg nature controlling line in the position of detection window, CCP antigen detection line and sheep anti-mouse igg nature controlling line place, described goat anti-rabbit igg nature controlling line, CCP antigen detection line and sheep anti-mouse igg nature controlling line and region, left and right thereof is made to be in detection window, the position at the corresponding sample pad place, position of well, makes sample can be added drop-wise in sample pad.
In described check-out console sample pad can adopt whole blood pad, glass, nitrocellulose filter, adsorptive pads and other have in the sample pad of similar action one or more.
CCP antigen concentration in described check-out console is 1.0mg/ml-3.0mg/ml, as capture antigen.
The utility model can be applicable to but is not limited to semi-automatic and full-automatic POCT immunoassay instrument, and minute is short, and result is accurate, highly sensitive.Almost identical with the cost that great amount of samples detects to single sample, be therefore applicable to detection that is a small amount of and batch sample.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of embodiment provided by the utility model.
Fig. 2 is the schematic cross-section of check-out console.
Embodiment
Below by embodiment, the utility model is described in further detail, but the utility model is not only confined to following examples.
With reference to Fig. 1 and 2, check-out console for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit provided by the utility model, comprise base plate 7, the middle part of base plate 7 arranges nitrocellulose filter 2, nitrocellulose filter 2 is provided with and is separated from each other and the goat anti-rabbit igg nature controlling line 3 arranged successively of left and right, CCP antigen detection line 4 and sheep anti-mouse igg nature controlling line 5, described goat anti-rabbit igg nature controlling line 3, CCP antigen detection line 4 and sheep anti-mouse igg nature controlling line 5 are coated on nitrocellulose filter, the side of sample pad 1 covers nitrocellulose filter 2 on this side residing for goat anti-rabbit igg nature controlling line 3, the side of adsorptive pads 6 covers on the opposite side of nitrocellulose filter 2.
Described check-out console has shell, is plastic material, described shell two pieces of dividing upper cover 8 and lower cover 9 to link together, and upper cover 8 is provided with well 10 and detection window 11; The corresponding detection line 4 in position of detection window and the position at nature controlling line 5 place, described goat anti-rabbit igg nature controlling line 3, CCP antigen detection line 4 and sheep anti-mouse igg nature controlling line 5 and region, left and right thereof is made to be in detection window, the position at corresponding sample pad 1 place, position of well, makes sample can be added drop-wise in sample pad.
Cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit by check-out console, detect damping fluid, quality-control product and contain the chip of calibration curve or Quick Response Code is formed.
Described detection damping fluid comprises fluorescently-labeled anti-human IgG antibodies, fluorescently-labeled rabbit igg, PBS, surfactant, antiseptic.
Containing class serum component and recombinant C CP antibody in described quality-control product, antiseptic.
The chip of described calibration curve or Quick Response Code comprise information, the title of kit, lot number, the term of validity of calibration curve.
Fluorescently-labeled anti-human IgG antibodies in described detection damping fluid is the tracer antibody that CCP antibody can be had to combine.
PBS in described detection damping fluid is the one of four salt phosphate buffers, three salt phosphate buffers and disalt phosphate buffer, preferably four salt phosphate buffers, and PO4-3 concentration is 0.01mol/L, and pH value is 7.2-7.4.
Surfactant in described detection damping fluid is Tween-20, and its concentration is 0.1%-2%, can reduce surface tension, reduces non-specific binding, improves fluorescence antibody stability in the solution, and it is even to promote to run plate.
Antiseptic in described detection damping fluid is the one of Sodium azide, Proclin150 and Proclin300, preferred Sodium azide, and its concentration is 0.01%-0.1%.
Class serum component in described quality-control product is used for imitating blood of human body environment.
CCP antibody in described quality-control product is can simplify albumen with the pole of CCP antigen and anti-human igg specific binding respectively.
Described quality-control product is the quality-control product of the amount of an including but not limited to concentration.
The utility model achieves the detection perform of high specific, high sensitivity, pin-point accuracy.Sentence read result is easy, only needs several minutes; Condition is provided for rapid screening RA realizes early diagnosis early treatment; Meet the demand of laboratories, instant detection, bedside detection.
Claims (2)
1., for a check-out console for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit, it is characterized in that:
Comprise base plate (7), the middle part of base plate (7) arranges nitrocellulose filter (2), nitrocellulose filter (2) is provided with and is separated from each other and the goat anti-rabbit igg nature controlling line (3) arranged successively of left and right, CCP antigen detection line (4) and sheep anti-mouse igg nature controlling line (5), described goat anti-rabbit igg nature controlling line (3), CCP antigen detection line (4) and sheep anti-mouse igg nature controlling line (5) are coated on nitrocellulose filter, the side of sample pad (1) covers nitrocellulose filter (2) on this side residing for goat anti-rabbit igg nature controlling line (3), the side of adsorptive pads (6) covers on the opposite side of nitrocellulose filter (2).
2. a kind of check-out console for cyclic citrullinated peptid fluorescence immune chromatography standard measure detection kit as claimed in claim 1, it is characterized in that described check-out console has shell, for plastic material, described shell two pieces of dividing upper cover (8) and lower cover (9) to link together, upper cover (8) is provided with well (10) and detection window (11); The position at corresponding goat anti-rabbit igg nature controlling line (3) in the position of detection window, CCP antigen detection line (4) and sheep anti-mouse igg nature controlling line (5) place, described goat anti-rabbit igg nature controlling line (3), CCP antigen detection line (4) and sheep anti-mouse igg nature controlling line (5) and region, left and right thereof is made to be in detection window, the position at corresponding sample pad (1) place, position of well, makes sample can be added drop-wise in sample pad.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520548925.XU CN204903546U (en) | 2015-07-27 | 2015-07-27 | A pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520548925.XU CN204903546U (en) | 2015-07-27 | 2015-07-27 | A pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit |
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| CN204903546U true CN204903546U (en) | 2015-12-23 |
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| CN201520548925.XU Active CN204903546U (en) | 2015-07-27 | 2015-07-27 | A pick -up plate for resisting ring citrulline peptide antibody fluorescence immunity chromatography method quantitative determination kit |
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| CN (1) | CN204903546U (en) |
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- 2015-07-27 CN CN201520548925.XU patent/CN204903546U/en active Active
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Address after: 10, building 311100, building 519, Xingguo Road, Yuhang Economic Development Zone, Zhejiang, Hangzhou Patentee after: The Sheng Tai biotechnology Limited by Share Ltd Address before: 311100 No. 10, building 519, Xingguo Road, Qianjiang Economic Development Zone, Zhejiang, Hangzhou Patentee before: HANGZHOU JOINSTAR BIOTECHNOLOGY CO., LTD. |