CN204766705U - 3D prints medical porous metal support with little support of bionical three -dimensional - Google Patents
3D prints medical porous metal support with little support of bionical three -dimensional Download PDFInfo
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- CN204766705U CN204766705U CN201520264579.2U CN201520264579U CN204766705U CN 204766705 U CN204766705 U CN 204766705U CN 201520264579 U CN201520264579 U CN 201520264579U CN 204766705 U CN204766705 U CN 204766705U
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Abstract
The utility model discloses a 3D prints medical porous metal support with little support of bionical three -dimensional. The utility model discloses a 3D prints medical porous metal support with little support of bionical three -dimensional includes the porous metal support, is located the inside three -dimensional little support of porous metal support, the blood platelet layer of three -dimensional little support surface adhesion. The porous metal support all is the pore structure that cascades who communicates each other with three -dimensional little support. The utility model discloses a 3D prints medical porous metal support with little support of bionical three -dimensional has not only satisfied the needs to bone repair materials's mechanical strength, and grow into for the bone tissue and new hematal formation provides good growing environment moreover.
Description
Technical field
This utility model belongs to technical field of biomedical materials, relates to a kind of 3D with bionic three-dimensional micro rack and prints medical porous metal rack, is specifically related to one and makes it have bioactive porous metals support at three-dimensional micro rack surface attachment platelet layer.
Background technology
Titanium alloy, as one of bio-medical Metallic Functional Materials, is used widely in human body surgical implant, as mouth cavity planting body, Cranial defect patching material, pseudarthrosis implant etc.It has nontoxic, light weight, good biocompatibility and corrosion resistance and has the elastic modelling quantity more mated with Human Cortex's bone.And due to the biologically inert of titanium alloy surface, be unfavorable for Oesteoblast growth, therefore porous and sensitivity titanium alloy support receive increasing concern.But existing Machining Technology is difficult to prepare even micron level UNICOM of the same size hole.
The appearance titanium alloy that allowed people re-recognize that 3D prints, by the process of the scan-datas such as CT, built by Computer Design, titanium alloy can be printed as the personalized porous meeting demand and be communicated with labyrinth, not only provide space to Bone Ingrowth, also because loose structure reduces the elastic modelling quantity of titanium alloy, achieve and the mating of normal bone tissues elastic modelling quantity, and not mating of degradation time and body demand need not be worried, become the important directions that 3D is printed on orthopaedics development.
But the porous titanium alloy material that existing 3D printing technique is produced, pore diameter is about 300-2000 μm, for the cell of average diameter 10-20 μm, seems too spacious.And cell can only prolong attached hole wall seeks connections with growth, three-dimension layer secondary growth in whole hole can not be realized, fail fully effectively to utilize the space of POROUS TITANIUM inside.Previously a lot of research emphasis is to utilize the method such as acid and alkali corrosion, plating finishing Porous titanium or area load bioactie agent, seldom has research emphasis to transform inner for porous support two-dimensional space as three dimensions.And if our guess in each hole of POROUS TITANIUM, can build a bionic three-dimensional micro rack, by giving the propagation of cell, differentiation provides a good environment.
The platelet in human body source contains abundant cytokine, as platelet derived growth factor (PDGF), transforming growth factor (TGF-β), VEGF (VEGF) etc.Multinomial in vitro study confirms, hematoblastic concentration becomes positive correlation with bone marrow stroma stem cell, fibroblast proliferation with the generation of I-type collagen.Platelet rich plasma (PRP) is platelet concentrate, and it can discharge cytokine profiles, plays a significant role in promotion Bone Defect Repari, angiogenesis, soft tissue generation etc.And now clinically, platelet rich plasma is ripe application in department of stomatology implantation body and transplantation of heart valve etc.On the other hand, platelet lyophlization technology is in market and clinical middle application, and it can accomplish the permanently effective preservation of room temperature, there is no too large impact to biologically active pdgf, and will slowly can activate the release growth inside factor after platelet again aquation.Platelet merged and titanium alloy then can be made to have multiple biological activity into 3D printing porous titanium alloy support, the biotic factor in its platelet source, closer to human body requirement, there are some researches show that platelet fibrosis can reach slow release effect to a certain extent.
Summary of the invention
This utility model provides a kind of 3D with bionic three-dimensional micro rack and prints medical porous metal rack.The described 3D with bionic three-dimensional micro rack prints the platelet layer 3 that medical porous metal rack comprises porous metals support 1, is positioned at the three-dimensional micro rack 2 of described porous metals internal stent and three-dimensional micro rack 2 surface attachment.The 3D with bionic three-dimensional micro rack of the present utility model prints the needs that medical porous metal rack not only meets the mechanical strength for bone renovating material, and grows into for osseous tissue and the formation of neovascularity provides good growing environment.
Further, described porous metals support 1 and described three-dimensional micro rack 2 are all the cascade pore space structures be interconnected.
Further, the aperture of described porous metals support 1 is 300 μm-3000 μm.
Further, the aperture of described three-dimensional micro rack 2 is 80 μm-200 μm.
Further, the thickness of described platelet layer 3 is 5 μm-15 μm.
Further, porous metals support 1 of the present utility model can be porous titanium alloy support, porous pure titanium support, porous stainless steel bracket, porous cobalt alloy support.In specific embodiments of the present utility model, described porous metals support 1 is porous titanium alloy support.
Further, described three-dimensional micro rack 2 of the present utility model can be prepared from by macromolecular material and/or bioceramic.
Macromolecular material can be one or more in collagen, gelatin, fibroin albumen, zein, alginate, chitosan, hyaluronate sodium, sodium alginate, dextran sulfate, heparin sodium, chondroitin sulfate or keratan sulfate.Bioceramic can be one or more in nanometer hydroxyapatite, tricalcium phosphate or calcium phosphate.
In specific embodiment of the present utility model, described three-dimensional micro rack 2 is prepared from by gelatin.
Further, the platelet layer 3 that this utility model uses is made up of the platelet layer of platelet rich plasma lyophilizing.
Advantage of the present utility model and beneficial effect:
1, the 3D with bionic three-dimensional micro rack of the present utility model prints medical porous metal rack and can provide optimal cell growth environment, the design having abandoned metal rack in traditional view must meet the restriction in the most applicable Growth of Cells aperture, be prepared into macroscopic void and high porosity structure as far as possible, thus achieved the maximization of the minimized of metal and Bone Ingrowth amount; Interior three-dimensional micro rack is then no longer by the restriction first meeting mechanical strength, adopt the material being beneficial to osteogenesis most as far as possible, build short texture, there is cell recognition site, be applicable to the three-dimensional micro rack that cell seeks connections with growth, for cell offers the best growth microenvironment, thus the mutual supplement with each other's advantages both realizing.
2, the platelet layer of three-dimensional micro rack surface attachment that the 3D with bionic three-dimensional micro rack of the present utility model prints in medical porous metal rack is made up of the platelet layer obtained after platelet rich plasma frozen dried, can preserve for a long time at normal temperatures, can slowly activate the release cells factor after meeting water hydratable, make it possess certain biological activity.
3, gelatin micro rack of the present utility model employs heat cross-linking method and is prepared from, avirulence.Glutaraldehyde or EDC reagent were cross-linked constructed micro rack and can remain a small amount of chemical reagent in the past, and cell growth and human body produce toxicity.
Accompanying drawing explanation
Fig. 1 shows the porous metals support using 3D printing technique to prepare;
Fig. 2 shows the schematic diagram with the porous metals support of the three-dimensional micro rack of gelatin of attachment platelet layer of the present utility model;
Wherein, 1: porous metals support; 2, the three-dimensional micro rack of gelatin; 3: platelet layer.
Detailed description of the invention
Below in conjunction with specific embodiment, setting forth this utility model further, only for explaining this utility model, and can not be interpreted as restriction of the present utility model.
The experimental technique of unreceipted actual conditions in the following example, the usually conveniently conditioned disjunction condition examinations of advising according to manufacturer.
The reagent in unreceipted source in the following example, all can obtain from commercial routes.
The 3D that embodiment 1 one kinds has bionic three-dimensional micro rack prints medical porous metal rack
A kind of 3D with bionic three-dimensional micro rack prints medical porous metal rack 1, comprises porous metals support 1, is positioned at the platelet layer 3 of the three-dimensional micro rack 2 of the gelatin of porous metals support 1 inside and three-dimensional micro rack 2 surface attachment of gelatin.Above-mentioned porous metals support 1 and the three-dimensional micro rack 2 of above-mentioned gelatin are all the cascade pore space structures be interconnected.The aperture of porous metals support 1 is 300 μm; The aperture of the three-dimensional micro rack 2 of gelatin is 80 μm; The thickness of platelet layer 3 is 5 μm.
The 3D that embodiment 2 one kinds has bionic three-dimensional micro rack prints medical porous metal rack
A kind of 3D with bionic three-dimensional micro rack prints medical porous metal rack 1, comprises porous metals support 1, is positioned at the platelet layer 3 of the three-dimensional micro rack 2 of the gelatin of porous metals support 1 inside and three-dimensional micro rack 2 surface attachment of gelatin.Above-mentioned porous metals support 1 and the three-dimensional micro rack 2 of above-mentioned gelatin are all the cascade pore space structures be interconnected.The aperture of porous metals support 1 is 1000 μm; The aperture of the three-dimensional micro rack 2 of gelatin is 150 μm; The thickness of platelet layer 3 is 10 μm.
The 3D that embodiment 3 one kinds has bionic three-dimensional micro rack prints medical porous metal rack
A kind of 3D with bionic three-dimensional micro rack prints medical porous metal rack 1, comprises porous metals support 1, is positioned at the platelet layer 3 of the three-dimensional micro rack 2 of the gelatin of porous metals support 1 inside and three-dimensional micro rack 2 surface attachment of gelatin.Above-mentioned porous metals support 1 and the three-dimensional micro rack 2 of above-mentioned gelatin are all the cascade pore space structures be interconnected.The aperture of porous metals support 1 is 3000 μm; The aperture of the three-dimensional micro rack 2 of gelatin is 200 μm; The thickness of platelet layer 3 is 15 μm.
The 3D that embodiment 4 has a bionic three-dimensional micro rack prints the preparation of medical porous metal rack
1, the preparation of porous titanium alloy support
(1) CT image is imported the three-dimensional imaging software such as Mimics or CAD, obtain the 3-D view of targeted bony tissue, average Kong Zhuwei 100-1000 μm, aperture is 300-3000 μm, with regular hexahedron, the filling of regular dodecahedron unit, expand this image, obtain personalized porous and be communicated with three-dimensional digital model.
(2) adopting EOSM280 metal material 3D printer, with titanium alloy (Ti-6Al-4V) for raw material, printing porous titanium alloy support according to designing a model.
2, the preparation of the compound porous titanium alloy support of the three-dimensional micro rack of gelatin
Gelatin particle is added deionized water, and at 37 DEG C, in the magnetic stirring apparatus of 300r/min, 2h is to thoroughly dissolving, and final concentration is 1.5-20%; Get gelatine solution 5ml, add 1g solid sodium chloride, to be filled into after mixing in porous titanium alloy support prepared by step (1) and lyophilization; At 180 DEG C after heat cross-linking 24h, with distillation washing sodium chloride, and after drying, obtain the porous titanium alloy support with three-dimensional micro rack.
3, gelatin three-dimensional micro rack surface attachment platelet layer
(1) platelet rich plasma is prepared: first, adopt venous blood 10ml with the vacuum blood taking needle that anticoagulant is housed, with the centrifugal 10min of 2000r/min, divide two-layer, upper strata is multiple platelet-containing solution, shifts out in whole supernatant and lower floor float 1mm to empty centrifuge tube; Then, recentrifuge 2000r/min, 10min, remove 3/4 supernatant, is precipitated thing and 1/4 supernatant, obtains the platelet rich plasma mixed after piping and druming.
(2) the porous titanium alloy support with the three-dimensional micro rack of gelatin above-mentioned steps 2 prepared is after EOG sterilizing, platelet rich plasma is dripped to it in super-clean bench, blood plasma complete wetting is made to have the porous titanium alloy support of the three-dimensional micro rack of gelatin, and enclose in sterilizing bag, again for subsequent use after lyophilizing.
The above embodiment only have expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to this utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of this utility model patent should be as the criterion with claims.
Claims (5)
1. a 3D with bionic three-dimensional micro rack prints medical porous metal rack, it is characterized in that, described in there is bionic three-dimensional micro rack 3D print the platelet layer (3) that medical porous metal rack comprises porous metals support (1), is positioned at the inner three-dimensional micro rack (2) of described porous metals support (1) and three-dimensional micro rack surface attachment.
2. the 3D with bionic three-dimensional micro rack according to claim 1 prints medical porous metal rack, it is characterized in that, described porous metals support (1) and described three-dimensional micro rack (2) are all the cascade pore space structures be interconnected.
3. the 3D with bionic three-dimensional micro rack according to claim 1 prints medical porous metal rack, it is characterized in that, the aperture of described porous metals support (1) is 300 μm-3000 μm.
4. the 3D with bionic three-dimensional micro rack according to claim 1 prints medical porous metal rack, it is characterized in that, the aperture of described three-dimensional micro rack (2) is 80 μm-200 μm.
5. the 3D with bionic three-dimensional micro rack according to claim 1 prints medical porous metal rack, it is characterized in that, the thickness of described platelet layer (3) is 5 μm-15 μm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105796214A (en) * | 2016-03-08 | 2016-07-27 | 吴志宏 | Porous metal cervical interbody fusion cage for directionally and slowly releasing rhBMP-2 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105796214A (en) * | 2016-03-08 | 2016-07-27 | 吴志宏 | Porous metal cervical interbody fusion cage for directionally and slowly releasing rhBMP-2 |
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Effective date of registration: 20180731 Address after: 100730 Wangfujing, Dongcheng District, Dongcheng District, Beijing Patentee after: Beijing Xiehe Hospital, Chinese Academy of Medical Sciences Address before: 100730 Peking Union Medical College Hospital, 1 Shuai Fu Garden, Dongcheng District, Beijing. Co-patentee before: Zhu Wei Patentee before: Wu Zhihong Co-patentee before: Weng Xisheng |
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| TR01 | Transfer of patent right |