CN204766036U - Animal cavum subarachnoidale device of dosing in succession - Google Patents
Animal cavum subarachnoidale device of dosing in succession Download PDFInfo
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- CN204766036U CN204766036U CN201520465114.3U CN201520465114U CN204766036U CN 204766036 U CN204766036 U CN 204766036U CN 201520465114 U CN201520465114 U CN 201520465114U CN 204766036 U CN204766036 U CN 204766036U
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Abstract
The utility model provides an animal cavum subarachnoidale device of dosing in succession, the device include pjncture needle, PE -10 pipe and pipe protective sheath, and the pjncture needle includes the outer needle of stainless steel lumbar anesthesia needle and dura mater, the length of the outer needle of dura mater be 70cm, external diameter for 1.6mm, incision face for personally submitting the inclined plane at 30 angle with the level, apart from incision face top 1cm department be provided with one long for 3 -6mm, width for 1 -1.5mm's side opening, the external diameter of PE -10 pipe is 0.6mm, the internal diameter is 0.3mm, length 6cm, and its periphery is placed and is provided with two rings form thrust apart from pipe terminal 1.5 -2.5cm department, the pipe protective sheath is that the major axis is 25mm, minor axis for 20mm's oval sheetmetal, and left one side of something of sheetmetal is equipped with the aperture of four diameters for 2.5 -3.5mm. The utility model provides a protection of opposition guan houwei pipe through improving, is strengthened to the device of dosing.
Description
Technical field
This utility model belongs to animal subarachnoid space tube indwelling technology field, particularly relates to animal subarachnoid space successive administration device.
Background technology
Spinal subarachnoid space in rats tube placing operation, is also referred to as sheath built-in pipe model, plays an important role in the experimentation of neuro physiology and pain therapy.
From Yaksh within 1976, having set up since spinal subarachnoid space in rats puts tube method, although mode and method are updated, also the method for many improvement is derived, such as through breast waist section sheath built-in pipe art and seal wire bootstrap technique, but still there are larger defect, such as spinal cord injury, cerebrospinal leak, postoperative infection, blood loss shock etc.Therefore how to improve put pipe and alleviate while being fixed into power spinal subarachnoid space in rats put the research of spinal cord injury in pipe be still endeavour both at home and abroad inquire into problem.Rise in the systematic research and application that sheath built-in pipe technology is carried out known since the eighties in last century, the excision of vertebral plate and spinous process often causes rat comparatively macrolesion, direct conduit removes to find epidural point of puncture usually and be not easy, and the degree of depth of puncture needle and conduit is not easy to grasp and causes spinal cord injury or put the excessively shallow problem of pipe.
Utility model content
the technical problem solved:in practical operation, easily damage is caused to rat for existing animal subarachnoid space successive administration device, point of puncture difficulty is sought, the shortcoming such as puncture needle and depth of catheter are not easily grasped, this utility model provides a kind of animal subarachnoid space successive administration device, and this device substantially increases animal subarachnoid space puncture and intubation and fixing success rate.
technical scheme:animal subarachnoid space successive administration device, this device comprises puncture needle, PE-10 conduit and conduit protective sleeve, described puncture needle comprises rustless steel spinal needle and epidural needle, the external diameter of rustless steel spinal needle is 0.7mm, length is 90mm, cut sides is inclined-plane, the length of epidural needle is 70cm, external diameter is 1.6mm, cut sides is inclined-plane, cut sides is horizontal by the angle of 30 °, distance 1cm place, cut sides top be provided with one long be 3-6mm, the wide side opening for 1-1.5mm, the hypotenuse of side opening shaft section is parallel with cut sides; The external diameter of described PE-10 conduit is 0.6mm, internal diameter is 0.3mm, length 6cm, and on its outside, distance catheter tip 1.5-2.5cm place is provided with two circular protrusion things, and the distance between two circular protrusion things is 2mm; The oval sheet metal that described conduit protective sleeve is major axis is 25mm, minor axis is 20mm, it is the aperture of 2.5-3.5mm that left one side of something of sheet metal is provided with four diameters, four apertures symmetry arrangement up and down.
Circular protrusion thing described above is sealing compound circular protrusion thing.
The length of side opening described above be 5mm, wide be 1.5mm.
On PE-10 conduit outside described above, distance catheter tip 2cm place is provided with two circular protrusion things.
The diameter of aperture described above is 3mm.
beneficial effect:the animal subarachnoid space successive administration device that this utility model provides, by improvement and the iron sheet fixing means of conduit, strengthens the protection of microtubular after opposed pipe.A large amount of results of animal display, this device is used to carry out putting pipe, can significantly improve and put pipe success rate, obviously reduce rat conduit extrusion rate simultaneously, reduce sensation and the dyskinesia that rat tube placing operation causes, improve subarachnoid space and put pipe and indwelling success rate, provide comparatively definite experiment support for the fields such as neurosurgery, anesthesiology and neuro pharmacology use subarachnoid space tube indwelling technology to use.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of epidural needle.
Fig. 2 is the top view of Fig. 1.
Fig. 3 is the schematic diagram of rustless steel spinal needle.
Fig. 4 is PE-10 conduit schematic diagram.
Fig. 5 is conduit protective sleeve schematic diagram.
Fig. 6 be embodiment 1 put pipe step schematic diagram.
In figure: 1, epidural needle, 2, cut sides, 3, side opening, 4, PE-10 conduit, 5, circular protrusion thing, 6, conduit protective sleeve, 7, aperture.
Detailed description of the invention
Rustless steel spinal needle described in the present embodiment, epidural needle 1 are purchased from Henan Tuo Ren medical apparatus and instruments Group Co., Ltd, and PE-10 conduit 4 is purchased from Ningbo An Lai Bioisystech Co., Ltd.
embodiment 1
Animal subarachnoid space successive administration device, this device comprises puncture needle, PE-10 conduit 4 and conduit protective sleeve 6, described puncture needle comprises rustless steel spinal needle and epidural needle 1, the external diameter of rustless steel spinal needle is 0.7mm, length is 90mm, cut sides 2 is inclined-plane, the external diameter of epidural needle 1 is 1.6mm, cut sides 2 is inclined-plane, the external diameter of described PE-10 conduit 4 is 0.6mm, internal diameter is 0.3mm, length 6cm, the length of described epidural needle 1 is 70cm, cut sides 2 is horizontal by the angle of 30 °, distance 1cm place, cut sides 2 top be provided with one long for 5mm, wide is the side opening 3 of 1.5mm, the hypotenuse of side opening 3 shaft section is parallel with cut sides 2, on described PE-10 conduit 4 outside, distance catheter tip 2cm place is provided with the distance between two sealing compound circular protrusion things, 5, two circular protrusion things 5 is 2mm, the oval sheet metal that described conduit protective sleeve 6 is major axis is 25mm, minor axis is 20mm, left one side of something of sheet metal is provided with aperture 7, four apertures 7 symmetry arrangement up and down that four diameters are 3mm.
Choose 50 rats to test, repetitive operation following steps:
Pipe step that what the animal subarachnoid space successive administration device utilizing this utility model to provide carried out put is as follows:
The chloral hydrate that rat abdominal cavity is 10% with the dosage injection concentration of 400mg/kg is anaesthetized.After anesthesia, shave off neck waist hair.Rat is fixed on operating-table with ventricumbent position, with a yoke, waist is arched upward.T12-L6 vertebra skin is shaved in a rectangular area (3 × 2cm) hair and uses povidone iodine sterilization.Centered by waist 3 ~ 4 gap, do along the other right side of spinous process longitudinal skin incision face that a length is 1cm, then by the paraspinal muscle that is attached on the right side of vertebra and spinous process blunt separation.First, insert epidural needle 1 and make most advanced and sophisticated arrival vertebral plate.Along vertebral plate upward sliding pin, find L3-4 intervertebral foramina, if arrived at, obvious resistance can be had.Make epidural needle 1 keep the angle with horizontal plane 20 °, with the angle of 30 °, center line, keep inclined-plane and groove upward, fix needle body with left hand.Insert rustless steel spinal needle with the right hand, puncture ligamenta flava and spinal dura mater gently, have one and significantly break through sense, can by rustless steel spinal needle left rotation and right rotation, to expand the diameter of puncturing hole.Remove rustless steel spinal needle, clamp sheath inner catheter with curved tweezers, the side opening 3 along epidural needle 1 inserts, and when PE-10 conduit 4 interface arrives at puncturing hole, has resistance.Slightly firmly make PE-10 conduit 4 through puncturing hole, and continue to push ahead 2cm, PE-10 conduit 4 joint portion will be stuck on hole, and the waist being placed on spinal cord also expands by the top of PE-10 conduit 4.A whipping can enter the signal of subarachnoid space as PE-10 conduit 4, and can see cerebrospinal fluid inflow catheter limpid on a small quantity.
The subcutaneous fixing step that the animal subarachnoid space successive administration device utilizing this utility model to provide carries out is as follows:
With chorda serica chirurgicalis sterilis, PE-10 conduit 4 and ridge paravertebral muscles are fixed; paravertebral muscles layer-by-layer suture; tie a knot between two circular protrusion things 5 with chorda serica chirurgicalis sterilis; PE-10 conduit 4 is fixed on subcutaneous tissue; in outlet marker pen mark depths; by PE-10 conduit 4 outer end through the aperture 7 on conduit protective sleeve 6, insert in the middle of conduit protective sleeve 6, close with aseptic steel wire after rinsing with 5 μ L physiological saline solution.Then skin incision face is sewed up.Wear silk thread with suture needle through skin and superficial fascia, conduit protective sleeve 6 stitching to be fixed on around rat back operative incision face.Fix rear craft by conduit protective sleeve 6 opisthotonos 90 °, PE-10 conduit 4 is covered completely, thus avoids baiting of rat.For protecting from infection, whole operation process and associated surgical instrument strictly perform by aseptic operation requirement.Rat is put Guan Houdan cage and raises completing subarachnoid space, and is used as following experiment, and every rat is all only used as once to test.
Comparative example 1
Animal subarachnoid space successive administration device, this device comprises PE-10 conduit 4, and its external diameter is 0.6mm, internal diameter is 0.3mm, length 6cm.
Do not use puncture needle and conduit protective sleeve.
Choose 50 rats to test, operating procedure is as follows:
(this step comes from WangBC to the classical step described according to Wang etc., HillmanDE, LiD, TurndorfH.Lumbarsubarachnoidcatheterizationinrats.Pharma colBiochemBehav1991; 38:685-8), carry out waist section vertebra subarachnoid space and put pipe, namely via the cerebral dura mater of the postoperative exposure of vertebrae plate resection, import PE-10 conduit 4.Other operating procedures are identical with embodiment 1 step.
After putting pipe 3d, by concentration be 2% lignocaine 20 μ L inject the limbs of embodiment 1 and comparative example 1 without in obvious dyskinetic rat sheath with the speed of 0.5mL/s, onset time of the observation two hindlimb paralysis time of two hindlimb paralysis (after the injection 20 μ L) and effective time (after injection, onset also can support the time of body weight to exercise recovery), and calculate the effective percentage rate of drug effect.In order to show that this conduit itself does not damage spinal cord or causes the change of behavior, we use sensation and the exercise testing of standard, respectively before surgery with Post operation the 1st, 3 and 7d, measure the thermal hyperalgesia reaction of two groups of rats, mechanical hyperalgesia reaction, tail-flick test and motor coordination function.At the end of experiment, dissect all death under the microscope and put to death rat and observe spinal cord injury, hematoma and catheter position, and the spinal cord of rat with histology process.
The operating time of embodiment 1 and comparative example 1 is respectively 21.68 ± 9.11min and 41.42 ± 7.19min.Compared with comparative example 1, the operating time of embodiment 1 obviously shortens (P < 0.05).Based on lidocaine test, we obtain a sheath interpolation pipe success rate is 97.9%.Fix for conduit, comparative example 1 has a certain proportion of pipe to damage or pulls out or retract subcutaneous, cannot further test.On the contrary, in embodiment 1, the de-pipe that only had to be found.This shows, embodiment 1 has obvious advantage compared with comparative example 1.
The 21 day after surgery, in embodiment 1, the mortality rate of rat was 5%, was 14% of rats death rate in comparative example 1.Lidocaine test and postoperative anatomical results show, the conduit of embodiment 1 is positioned at side or opposite side (left or right) subarachnoid space at the tip of the rat of 79%.By WMT, TWL and Rota-Rod experiment, the method for embodiment 1 does not affect rat Pain behaviour and motor coordination.
Embodiment 1 and the experimental data of comparative example 1 see the following form 1 and table 2.
Table 1: the sheath built-in pipe success rate of embodiment 1 and comparative example 1 and the comparison of related complication
Table 2: the impact on machinery and burning pain threshold and motor function of embodiment 1 and comparative example 1
Claims (5)
1. animal subarachnoid space successive administration device, it is characterized in that: this device comprises puncture needle, PE-10 conduit (4) and conduit protective sleeve (6), described puncture needle comprises rustless steel spinal needle and epidural needle (1), the external diameter of rustless steel spinal needle is 0.7mm, length is 90mm, cut sides (2) is inclined-plane, the length of epidural needle (1) is 70cm, external diameter is 1.6mm, cut sides (2) is inclined-plane, cut sides (2) is horizontal by the angle of 30 °, distance 1cm place, cut sides (2) top be provided with one long for 3-6mm, wide is the side opening (3) of 1-1.5mm, the hypotenuse of side opening (3) shaft section is parallel with cut sides (2), the external diameter of described PE-10 conduit (4) is 0.6mm, internal diameter is 0.3mm, length 6cm, and on its outside, distance catheter tip 1.5-2.5cm place is provided with two circular protrusion things (5), and the distance between two circular protrusion things (5) is 2mm, the oval sheet metal that described conduit protective sleeve (6) is major axis is 25mm, minor axis is 20mm, left one side of something of sheet metal is provided with the aperture (7) that four diameters are 2.5-3.5mm, four apertures (7) symmetry arrangement up and down.
2. animal subarachnoid space successive administration device according to claim 1, is characterized in that: described circular protrusion thing (5) is sealing compound circular protrusion thing (5).
3. animal subarachnoid space successive administration device according to claim 1, is characterized in that: the length of described side opening (3) be 5mm, wide be 1.5mm.
4. animal subarachnoid space successive administration device according to claim 1, is characterized in that: on described PE-10 conduit (4) outside, distance catheter tip 2cm place is provided with two circular protrusion things (5).
5. animal subarachnoid space successive administration device according to claim 1, is characterized in that: the diameter of described aperture (7) is 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520465114.3U CN204766036U (en) | 2015-07-02 | 2015-07-02 | Animal cavum subarachnoidale device of dosing in succession |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520465114.3U CN204766036U (en) | 2015-07-02 | 2015-07-02 | Animal cavum subarachnoidale device of dosing in succession |
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| CN204766036U true CN204766036U (en) | 2015-11-18 |
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| CN201520465114.3U Expired - Fee Related CN204766036U (en) | 2015-07-02 | 2015-07-02 | Animal cavum subarachnoidale device of dosing in succession |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106215276A (en) * | 2016-08-31 | 2016-12-14 | 北京兆仕医疗器械有限责任公司 | A kind of subarachnoid space epidural canalis spinalis doser |
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2015
- 2015-07-02 CN CN201520465114.3U patent/CN204766036U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106215276A (en) * | 2016-08-31 | 2016-12-14 | 北京兆仕医疗器械有限责任公司 | A kind of subarachnoid space epidural canalis spinalis doser |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151118 Termination date: 20190702 |