CN204752728U - Soak sealed in situ hybridization box entirely - Google Patents
Soak sealed in situ hybridization box entirely Download PDFInfo
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- CN204752728U CN204752728U CN201520410802.XU CN201520410802U CN204752728U CN 204752728 U CN204752728 U CN 204752728U CN 201520410802 U CN201520410802 U CN 201520410802U CN 204752728 U CN204752728 U CN 204752728U
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- sidewall
- chassis body
- ring
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Abstract
The utility model discloses a soak sealed in situ hybridization box entirely, comprises a box bod, the box body include the chassis body, with the closing cap body of chassis body adaptation, its characterized in that: being equipped with on the body of chassis and holding the chamber, the inner wall that holds the chamber extends towards the opening direction of holding the chamber and forms the lateral wall, be equipped with on the closing cap body with the recess of lateral wall adaptation, be equipped with the sealing washer between the outer peripheral face of lateral wall and the inner wall of recess, the lateral wall on the body of chassis forms sealed cooperation of pegging graft with the recess on the closing cap body. Not only the leakproofness is good to adopt above -mentioned structure, can wholely soak in other experimental environment such as gas bath or water bath moreover for the heat preservation and the moisturizing effect of hybridization box are better, small, convenient to use, and the range of application is wide.
Description
Technical field
The utility model belongs to biological technical field, the sealed hybridization histochemistry box of especially a kind of complete immersion for RNA molecule in situ hybridization.
Background technology
At present, RNA molecule in situ hybridization is the experimental technique that biomedical sector is conventional, is mainly used in measuring the expression in vivo of certain gene, can be used for pathological diagnosis clinically.In this experimental implementation process, the biological tissue samples on a small amount of molecular probe solution and slide glass, by hatching for a long time, is hybridized with specific gene, to determine gene expression in vivo.Molecular hybridization requires long hot environment, and this brings very large challenge with regard to giving the heat and moisture preserving of sample.In prior art, CN02221709 hybridization histochemistry and the RNA molecule in situ hybridization box described in immunohistochemistry moisture preservation box comprise the glass film plates of equal thickness, glass floor is provided with the flat glass row culture sidewall being bonded at glass film plates surrounding and forms container cavity, sidewall is provided with the glass lid abutted with sidewall end face, said structure is adopted to have following shortcoming as the hybridizing box of RNA molecule in situ hybridization: one is that glass hybridizing box does not arrange any sealed structure, cannot accomplish to seal completely, sample is moisturizing not easily, the very fast evaporation drying of molecular probe solution, impact experiment, two is that hybridization temperature is subject to external environment influence, poor stability, three be glass lid and sidewall relative smooth without any locating structure so glass lid is easily shifted, the material in hybridizing box is revealed.
Utility model content
In order to overcome the deficiencies in the prior art, the utility model provide a kind of than prior art heat and moisture preserving the effective and sealed hybridization histochemistry box of the complete immersion of good stability.
To achieve these goals, the technical solution adopted in the utility model is: the sealed hybridization histochemistry box of a kind of complete immersion, comprise box body, described box body comprise chassis body, with the closure body of chassis body adaptation, it is characterized in that: described chassis body is provided with container cavity, the inwall of container cavity extends to form sidewall towards the opening direction of container cavity, closure body is provided with the groove with sidewall adaptation, be provided with sealing-ring between the periphery of sidewall and the inwall of groove, the sidewall in chassis body and the groove on closure body form grafting and are sealed and matched.
In said structure, adopt sealing-ring between chassis body and closure body to seal, effectively improve sealed structure, and chassis body and closure body are all made by lathe cut, ensure that the smooth of trim, solve hybridizing box in prior art cannot seal completely, the problem of sample not easily moisturizing, simplify production process and cost simultaneously, filter paper can be placed in container cavity, the experiment such as slide glass and hybridization solution article, sidewall mainly plays effect that is fixing and location, prevent the liquid in container cavity from leaching because of too high in addition, due to good airproof performance, can wholely be immersed in gas bath or water-bath, in so avoiding the evaporation of hybridization solution, improve the effect of moisturizing, also the better heat preservation of hybridizing box can be made.
As further setting of the present utility model, the outer wall of described sidewall and the end face of chassis body junction form L-type step surface, and sealing-ring is set on the bottom surface of this L-type step surface; The inwall at described slot opening place is provided with the ring groove that can hold sealing-ring, and the outer ring surface of sealing-ring abuts with the sidewall of this ring groove, and sidewall outer wall abuts with groove inner wall.
In said structure, after chassis body and closure body seal closure, the cavity inner wall that sealing-ring is formed with L-type step surface and ring groove abuts completely, and make sealing tightr like this, sealing effectiveness is better.
As further setting of the present utility model, the periphery of described chassis body stretches out formation first annular disc, the periphery of closure body stretches out formation second annular disc, first annular disc and the second annular disc are all dispersed with mutually adaptive threaded hole, chassis body and closure body are bolted to connection, and one end of bolt is passed the threaded hole of closure body and chassis body successively and extends formation feet.
In said structure, adopting bolt to be connected and fixed chassis body and closure body can not only flexible operating, and ensure that the stopping property of in situ hybridization box, the feet that the bolt exceeded is formed is positioned at the centre of water-bath liquid when hybridizing box can also be made to work, the liquid of hybridizing box upper and lower surface is fully exchanged, temperature is consistent especially up and down to ensure that hybridizing box each several part, thus prevents the evaporation of hybridization solution under high temperature.
As further setting of the present utility model, described first annular disc is towards the end face of closure body and the second annular disc towards the end contact of chassis body.
In said structure, the first annular disc and the second annular disc plane-plane contact, illustrate that hybridizing box of the present utility model is all-sealed, better tightness, and the damage decreasing sealing-ring.
As further setting of the present utility model, described chassis body and closure body are made by stainless material, and chassis body and closure body are collar plate shape.
In said structure, the in situ hybridization box body that chassis body and closure body are formed amasss little, easy to use, owing to being stainless material, be heated evenly, the temperature at each position of hybridizing box is all consistent, require low to warming-up device, directly can be immersed in the conventional water-bath with temperature controlling system and carry out hybridization use, applied range.
Adopt such scheme, not only good airproof performance, and can be wholely immersed in other experimental situations such as gas bath or water-bath, make the insulation of hybridizing box and humidity-holding effect better, volume is little, easy to use, applied range.
Below in conjunction with accompanying drawing, the utility model is further described.
Accompanying drawing explanation
Accompanying drawing 1 is the utility model specific embodiment schematic appearance;
Accompanying drawing 2 is the utility model specific embodiment STRUCTURE DECOMPOSITION figure;
Accompanying drawing 3 is the utility model specific embodiment structure sectional view;
Accompanying drawing 4 is the utility model specific embodiment structure sectional view.
Embodiment
Below by embodiment, the utility model is specifically described; only for being further described the utility model; can not be interpreted as the restriction to the utility model protection domain, the technician in this field can make some nonessential improvement and adjustment according to the content of above-mentioned utility model to the utility model.
Specific embodiment of the utility model as Figure 1-4, the sealed hybridization histochemistry box of a kind of complete immersion, comprise box body, described box body comprise chassis body 1, with the closure body 2 of chassis body 1 adaptation, described chassis body 1 is provided with container cavity 11, the inwall of container cavity 11 extends to form sidewall 12 towards the opening direction of container cavity 11, closure body 2 is provided with the groove 21 with sidewall 12 adaptation, be provided with sealing-ring 3 between the periphery of sidewall 12 and the inwall of groove 21, the sidewall 12 in chassis body 1 forms grafting with the groove 21 on closure body 2 and is sealed and matched.Sealing-ring 3 is adopted to seal between chassis body 1 and closure body 2, effectively improve sealed structure, and chassis body 1 and closure body 3 are all made by lathe cut, ensure that the smooth of trim, solve hybridizing box in prior art cannot seal completely, the problem of sample not easily moisturizing, simplify production process and cost simultaneously, filter paper can be placed in container cavity 11, the experiment such as slide glass and hybridization solution article, sidewall 12 mainly plays effect that is fixing and location, prevent the liquid in container cavity 11 from leaching because of too high in addition, due to good airproof performance, can wholely be immersed in gas bath or water-bath, in so avoiding the evaporation of hybridization solution, improve the effect of moisturizing, also the better heat preservation of hybridizing box can be made.
The outer wall of above-mentioned sidewall 12 and the end face of chassis body 1 junction form L-type step surface 13, and sealing-ring 3 is set on the bottom surface of this L-type step surface 13; The inwall of described groove 21 opening part is provided with the ring groove 22 that can hold sealing-ring 3, and the outer ring surface of sealing-ring 3 abuts with the sidewall of this ring groove 22, and sidewall 12 outer wall abuts with groove 21 inwall.
The periphery of above-mentioned chassis body 1 stretches out formation first annular disc 14, the periphery of closure body 2 stretches out formation second annular disc 23, first annular disc 14 and the second annular disc 23 are all dispersed with mutually adaptive threaded hole 141(231), chassis body 1 is fixedly connected with by bolt 4 with closure body 2, and one end of bolt 4 passes the threaded hole 231(141 of closure body 2 and chassis body 1 successively) and extend formation feet.Adopting bolt 4 to be connected and fixed chassis body 1 and closure body 2 can not only flexible operating, and ensure that the stopping property of in situ hybridization box, the feet that the bolt 4 exceeded is formed is positioned at the centre of water-bath liquid when hybridizing box can also be made to work, the liquid of hybridizing box upper and lower surface is fully exchanged, temperature is consistent especially up and down to ensure that hybridizing box each several part, thus prevents the evaporation of hybridization solution under high temperature.
Above-mentioned first annular disc 14 is towards the end face of closure body 2 and the second annular disc 23 towards the end contact of chassis body 1.First annular disc 14 and the second annular disc 23 plane-plane contact, illustrates that hybridizing box of the present utility model is all-sealed, better tightness, and the damage decreasing sealing-ring 3.
Above-mentioned chassis body 1 and closure body 2 are made by stainless material, and chassis body 1 and closure body 2 are collar plate shape.The in situ hybridization box body that chassis body 1 and closure body 2 are formed amasss little, easy to use, owing to being stainless material, be heated evenly, the temperature at each position of hybridizing box is all consistent, require low to warming-up device, directly can be immersed in the conventional water-bath with temperature controlling system and carry out hybridization use, applied range.
Claims (5)
1. the sealed hybridization histochemistry box of complete immersion, comprise box body, described box body comprise chassis body, with the closure body of chassis body adaptation, it is characterized in that: described chassis body is provided with container cavity, the inwall of container cavity extends to form sidewall towards the opening direction of container cavity, closure body is provided with the groove with sidewall adaptation, is provided with sealing-ring between the periphery of sidewall and the inwall of groove, and the sidewall in chassis body and the groove on closure body form grafting and be sealed and matched.
2. the sealed hybridization histochemistry box of a kind of complete immersion according to claim 1, is characterized in that: the outer wall of described sidewall and the end face of chassis body junction form L-type step surface, and sealing-ring is set on the bottom surface of this L-type step surface; The inwall at described slot opening place is provided with the ring groove that can hold sealing-ring, and the outer ring surface of sealing-ring abuts with the sidewall of this ring groove, and sidewall outer wall abuts with groove inner wall.
3. the sealed hybridization histochemistry box of a kind of complete immersion according to claim 1, it is characterized in that: the periphery of described chassis body stretches out formation first annular disc, the periphery of closure body stretches out formation second annular disc, first annular disc and the second annular disc are all dispersed with mutually adaptive threaded hole, chassis body and closure body are bolted to connection, and one end of bolt is passed the threaded hole of closure body and chassis body successively and extends formation feet.
4. according to the sealed hybridization histochemistry box of a kind of complete immersion described in claim 3, it is characterized in that: described first annular disc is towards the end face of capping and the second annular disc towards the end contact on chassis.
5. the sealed hybridization histochemistry box of a kind of complete immersion according to claim 1-4 any one, it is characterized in that: described chassis body and closure body are made by stainless material, chassis body and closure body are collar plate shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520410802.XU CN204752728U (en) | 2015-06-15 | 2015-06-15 | Soak sealed in situ hybridization box entirely |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520410802.XU CN204752728U (en) | 2015-06-15 | 2015-06-15 | Soak sealed in situ hybridization box entirely |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204752728U true CN204752728U (en) | 2015-11-11 |
Family
ID=54467457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520410802.XU Expired - Fee Related CN204752728U (en) | 2015-06-15 | 2015-06-15 | Soak sealed in situ hybridization box entirely |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204752728U (en) |
-
2015
- 2015-06-15 CN CN201520410802.XU patent/CN204752728U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151111 Termination date: 20160615 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |